UniProtKB - Q08108 (PLB3_YEAST)
Protein
Lysophospholipase 3
Gene
PLB3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Sequentially removes both fatty acyl groups from diacylglycerophospholipids and therefore has both phospholipase A and lysophospholipase activities. Substrate preference is phosphatidylserine > phosphatidylinositol. Does not cleave phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid and phosphatidylinositol-bisphosphate (PubMed:10497163). Mainly responsible for the degradation of phosphatidylinositol in vivo (PubMed:15588231).2 Publications
Catalytic activityi
- EC:3.1.1.5
GO - Molecular functioni
- lysophospholipase activity Source: SGD
- phosphatidyl phospholipase B activity Source: UniProtKB-EC
- phospholipase A2 activity Source: GO_Central
GO - Biological processi
- glycerophospholipid catabolic process Source: GO_Central
- phosphatidylinositol metabolic process Source: SGD
- phosphatidylserine catabolic process Source: SGD
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid degradation, Lipid metabolism |
Enzyme and pathway databases
BioCyci | MetaCyc:YOL011W-MONOMER |
Reactomei | R-SCE-111995, phospho-PLA2 pathway R-SCE-1482788, Acyl chain remodelling of PC R-SCE-1482798, Acyl chain remodeling of CL R-SCE-1482801, Acyl chain remodelling of PS R-SCE-1482839, Acyl chain remodelling of PE R-SCE-1482922, Acyl chain remodelling of PI R-SCE-1482925, Acyl chain remodelling of PG R-SCE-1483115, Hydrolysis of LPC R-SCE-1483152, Hydrolysis of LPE R-SCE-1483166, Synthesis of PA R-SCE-2142753, Arachidonic acid metabolism R-SCE-418592, ADP signalling through P2Y purinoceptor 1 R-SCE-432142, Platelet sensitization by LDL R-SCE-6811436, COPI-independent Golgi-to-ER retrograde traffic |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PLB3 Ordered Locus Names:YOL011W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005371, PLB3 |
VEuPathDBi | FungiDB:YOL011W |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; GPI-anchor Curated
Cytosol
- cytosol Source: GO_Central
Extracellular region or secreted
- extracellular region Source: SGD
Plasma Membrane
- plasma membrane Source: GO_Central
Vacuole
- fungal-type vacuole Source: SGD
Other locations
- anchored component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: GO_Central
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Sequence analysisAdd BLAST | 26 | |
ChainiPRO_0000024648 | 27 – 659 | Lysophospholipase 3Add BLAST | 633 | |
PropeptideiPRO_0000372444 | 660 – 686 | Removed in mature formSequence analysisAdd BLAST | 27 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 56 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 82 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 129 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 166 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 221 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 283 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 313 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 351 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 495 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 519 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 547 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 571 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 588 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 614 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 659 | GPI-anchor amidated asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, GPI-anchor, LipoproteinProteomic databases
MaxQBi | Q08108 |
PaxDbi | Q08108 |
PRIDEi | Q08108 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 34393, 89 interactors |
DIPi | DIP-4220N |
IntActi | Q08108, 1 interactor |
MINTi | Q08108 |
STRINGi | 4932.YOL011W |
Miscellaneous databases
RNActi | Q08108, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 592 | PLA2cPROSITE-ProRule annotationAdd BLAST | 554 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 627 – 643 | Poly-SerAdd BLAST | 17 |
Sequence similaritiesi
Belongs to the lysophospholipase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1325, Eukaryota |
GeneTreei | ENSGT01000000214502 |
HOGENOMi | CLU_014602_0_0_1 |
InParanoidi | Q08108 |
OMAi | DGQNVPF |
Family and domain databases
InterProi | View protein in InterPro IPR016035, Acyl_Trfase/lysoPLipase IPR002642, LysoPLipase_cat_dom |
Pfami | View protein in Pfam PF01735, PLA2_B, 1 hit |
SMARTi | View protein in SMART SM00022, PLAc, 1 hit |
SUPFAMi | SSF52151, SSF52151, 1 hit |
PROSITEi | View protein in PROSITE PS51210, PLA2C, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q08108-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIRPLCSKII ISYIFAISQF LLAANAWSPT DSYVPGTVSC PDDINLVREA
60 70 80 90 100
TSISQNESAW LEKRNKVTSV ALKDFLTRAT ANFSDSSEVL SKLFNDGNSE
110 120 130 140 150
NLPKIAVAVS GGGYRSMLTG AGVLAAMDNR TEGAYEHGLG GLLQSTTYLS
160 170 180 190 200
GASGGNWLVG TLALNNWTSV QDILNNMQND DSIWDLSDSI VTPGGINIFK
210 220 230 240 250
TAKRWDHISN AVESKQNADY NTSLADIWGR ALAYNFFPSL NRGGIGLTWS
260 270 280 290 300
SIRDFPVFQN AEMPFPISVA DGRYPGTKVI NLNATVFEFN PFEMGSWDPS
310 320 330 340 350
LNSFANVKYL GTNVSNGVPL ERGKCTAGFD NAGFIMGTSS TLFNQFLLRI
360 370 380 390 400
NSTHLPSFIT RLARHFLKDL SQDFNDIAVY SPNPFKDTKF LDSDYTTSIV
410 420 430 440 450
DSDSLFLVDG GEDDENVPVL PLIQKERDVD IIFAVDNSAD MRLAWPDGSS
460 470 480 490 500
LVHTYERQFV KQGQGMSFPY VPDTNTFVNL GLNKKPTFFG CDANNLTDLQ
510 520 530 540 550
YIPPLVVYLP NAEYSFNSNQ SAFKLSYSES QRRSMIQNGF EIATRNNFTD
560 570 580 590 600
DPEFMGCVGC AIIRRKQQAL NITLPPECET CFKNYCWNGT LDTTPLPDVE
610 620 630 640 650
KDVHHSFINV NSFNSSIGQE ESLYAGSSAS QSSSSSSSSS SSSEIPSATA
660 670 680
TLEKKAATNS GSHLSGISVK FSAMIMLTLL MFTGAV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z74753 Genomic DNA Translation: CAA99010.1 BK006948 Genomic DNA Translation: DAA10772.1 |
PIRi | S66693 |
RefSeqi | NP_014632.1, NM_001183265.1 |
Genome annotation databases
EnsemblFungii | YOL011W_mRNA; YOL011W; YOL011W |
GeneIDi | 854151 |
KEGGi | sce:YOL011W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z74753 Genomic DNA Translation: CAA99010.1 BK006948 Genomic DNA Translation: DAA10772.1 |
PIRi | S66693 |
RefSeqi | NP_014632.1, NM_001183265.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 34393, 89 interactors |
DIPi | DIP-4220N |
IntActi | Q08108, 1 interactor |
MINTi | Q08108 |
STRINGi | 4932.YOL011W |
Proteomic databases
MaxQBi | Q08108 |
PaxDbi | Q08108 |
PRIDEi | Q08108 |
Genome annotation databases
EnsemblFungii | YOL011W_mRNA; YOL011W; YOL011W |
GeneIDi | 854151 |
KEGGi | sce:YOL011W |
Organism-specific databases
SGDi | S000005371, PLB3 |
VEuPathDBi | FungiDB:YOL011W |
Phylogenomic databases
eggNOGi | KOG1325, Eukaryota |
GeneTreei | ENSGT01000000214502 |
HOGENOMi | CLU_014602_0_0_1 |
InParanoidi | Q08108 |
OMAi | DGQNVPF |
Enzyme and pathway databases
BioCyci | MetaCyc:YOL011W-MONOMER |
Reactomei | R-SCE-111995, phospho-PLA2 pathway R-SCE-1482788, Acyl chain remodelling of PC R-SCE-1482798, Acyl chain remodeling of CL R-SCE-1482801, Acyl chain remodelling of PS R-SCE-1482839, Acyl chain remodelling of PE R-SCE-1482922, Acyl chain remodelling of PI R-SCE-1482925, Acyl chain remodelling of PG R-SCE-1483115, Hydrolysis of LPC R-SCE-1483152, Hydrolysis of LPE R-SCE-1483166, Synthesis of PA R-SCE-2142753, Arachidonic acid metabolism R-SCE-418592, ADP signalling through P2Y purinoceptor 1 R-SCE-432142, Platelet sensitization by LDL R-SCE-6811436, COPI-independent Golgi-to-ER retrograde traffic |
Miscellaneous databases
PROi | PR:Q08108 |
RNActi | Q08108, protein |
Family and domain databases
InterProi | View protein in InterPro IPR016035, Acyl_Trfase/lysoPLipase IPR002642, LysoPLipase_cat_dom |
Pfami | View protein in Pfam PF01735, PLA2_B, 1 hit |
SMARTi | View protein in SMART SM00022, PLAc, 1 hit |
SUPFAMi | SSF52151, SSF52151, 1 hit |
PROSITEi | View protein in PROSITE PS51210, PLA2C, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PLB3_YEAST | |
Accessioni | Q08108Primary (citable) accession number: Q08108 Secondary accession number(s): D6W256 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - SIMILARITY comments
Index of protein domains and families