UniProtKB - Q08091 (CNN1_MOUSE)
Protein
Calponin-1
Gene
Cnn1
Organism
Mus musculus (Mouse)
Status
Functioni
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
GO - Molecular functioni
- actin binding Source: MGI
- calmodulin binding Source: UniProtKB-KW
GO - Biological processi
- actomyosin structure organization Source: InterPro
- negative regulation of vascular associated smooth muscle cell proliferation Source: MGI
Keywordsi
Molecular function | Actin-binding, Calmodulin-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Calponin-1Alternative name(s): Basic calponin Calponin H1, smooth muscle |
Gene namesi | Name:Cnn1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104979, Cnn1 |
Subcellular locationi
Cytoskeleton
- cytoskeleton Source: MGI
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204768 | 1 – 297 | Calponin-1Add BLAST | 297 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 170 | Phosphothreonine; by ROCK2By similarity | 1 | |
Modified residuei | 175 | Phosphoserine; by ROCK2By similarity | 1 | |
Modified residuei | 180 | Phosphothreonine; by ROCK2By similarity | 1 | |
Modified residuei | 184 | Phosphothreonine; by ROCK2By similarity | 1 | |
Modified residuei | 259 | Phosphothreonine; by ROCK2By similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3970 |
MaxQBi | Q08091 |
PaxDbi | Q08091 |
PeptideAtlasi | Q08091 |
PRIDEi | Q08091 |
PTM databases
iPTMneti | Q08091 |
PhosphoSitePlusi | Q08091 |
Expressioni
Tissue specificityi
Smooth muscle, and tissues containing significant amounts of smooth muscle.
Gene expression databases
Bgeei | ENSMUSG00000001349, Expressed in urinary bladder and 161 other tissues |
ExpressionAtlasi | Q08091, baseline and differential |
Genevisiblei | Q08091, MM |
Interactioni
Subunit structurei
Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1.
By similarityGO - Molecular functioni
- actin binding Source: MGI
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000001384 |
Miscellaneous databases
RNActi | Q08091, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 131 | Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST | 104 | |
Repeati | 164 – 189 | Calponin-like 1Add BLAST | 26 | |
Repeati | 204 – 229 | Calponin-like 2Add BLAST | 26 | |
Repeati | 243 – 268 | Calponin-like 3Add BLAST | 26 |
Sequence similaritiesi
Belongs to the calponin family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2046, Eukaryota |
GeneTreei | ENSGT00940000159680 |
HOGENOMi | CLU_055232_0_0_1 |
InParanoidi | Q08091 |
PhylomeDBi | Q08091 |
TreeFami | TF313921 |
Family and domain databases
CDDi | cd00014, CH, 1 hit |
Gene3Di | 1.10.418.10, 1 hit |
InterProi | View protein in InterPro IPR001997, Calponin/LIMCH1 IPR000557, Calponin_repeat IPR001715, CH-domain IPR036872, CH_dom_sf IPR003096, SM22_calponin |
Pfami | View protein in Pfam PF00402, Calponin, 3 hits PF00307, CH, 1 hit |
PRINTSi | PR00889, CALPONIN PR00888, SM22CALPONIN |
SMARTi | View protein in SMART SM00033, CH, 1 hit |
SUPFAMi | SSF47576, SSF47576, 1 hit |
PROSITEi | View protein in PROSITE PS01052, CALPONIN_1, 3 hits PS51122, CALPONIN_2, 3 hits PS50021, CH, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform Alpha (identifier: Q08091-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSAHFNRGP AYGLSAEVKN KLAQKYDHQR EQELREWIEG VTGRRIGNNF
60 70 80 90 100
MDGLKDGIIL CEFINKLQPG SVKKVNESTQ NWHQLENIGN FIKAITKYGV
110 120 130 140 150
KPHDIFEAND LFENTNHTQV QSTLLALASM AKTKGNKVNV GVKYAEKQER
160 170 180 190 200
RFEPEKLREG RNIIGLQMGT NKFASQQGMT AYGTRRHLYD PKLGTDQPLD
210 220 230 240 250
QATISLQMGT NKGASQAGMT APGTKRQIFE PGLGMEHCDT LNVSLQMGSN
260 270 280 290
KGASQRGMTV YGLPRQVYDP KYCLNPEYPE LSEPTHNHHP HNYYNSA
Isoform Beta (identifier: Q08091-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
217-256: Missing.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1L1SRV0 | A0A1L1SRV0_MOUSE | Calponin | Cnn1 | 238 | Annotation score: | ||
A0A1L1SUB2 | A0A1L1SUB2_MOUSE | Calponin | Cnn1 | 233 | Annotation score: | ||
A0A1L1SRL1 | A0A1L1SRL1_MOUSE | Calponin-1 | Cnn1 | 82 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 35 | R → E in AAB01453 (Ref. 2) Curated | 1 | |
Sequence conflicti | 35 | R → E in AAB01452 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000755 | 217 – 256 | Missing in isoform Beta. CuratedAdd BLAST | 40 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z19542 mRNA Translation: CAA79602.1 L49022 Genomic DNA Translation: AAB01453.1 L49022 Genomic DNA Translation: AAB01452.1 U28932 Genomic DNA Translation: AAC52448.1 U40351 , U38930, U40348, U40349, U40350 Genomic DNA Translation: AAC52353.1 |
CCDSi | CCDS22921.1 [Q08091-1] |
PIRi | S36145, S31486 |
RefSeqi | NP_034052.3, NM_009922.4 [Q08091-1] |
Genome annotation databases
Ensembli | ENSMUST00000001384; ENSMUSP00000001384; ENSMUSG00000001349 [Q08091-1] |
GeneIDi | 12797 |
KEGGi | mmu:12797 |
UCSCi | uc009onv.2, mouse [Q08091-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z19542 mRNA Translation: CAA79602.1 L49022 Genomic DNA Translation: AAB01453.1 L49022 Genomic DNA Translation: AAB01452.1 U28932 Genomic DNA Translation: AAC52448.1 U40351 , U38930, U40348, U40349, U40350 Genomic DNA Translation: AAC52353.1 |
CCDSi | CCDS22921.1 [Q08091-1] |
PIRi | S36145, S31486 |
RefSeqi | NP_034052.3, NM_009922.4 [Q08091-1] |
3D structure databases
SMRi | Q08091 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000001384 |
PTM databases
iPTMneti | Q08091 |
PhosphoSitePlusi | Q08091 |
Proteomic databases
CPTACi | non-CPTAC-3970 |
MaxQBi | Q08091 |
PaxDbi | Q08091 |
PeptideAtlasi | Q08091 |
PRIDEi | Q08091 |
Protocols and materials databases
Antibodypediai | 1965, 1051 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000001384; ENSMUSP00000001384; ENSMUSG00000001349 [Q08091-1] |
GeneIDi | 12797 |
KEGGi | mmu:12797 |
UCSCi | uc009onv.2, mouse [Q08091-1] |
Organism-specific databases
CTDi | 1264 |
MGIi | MGI:104979, Cnn1 |
Phylogenomic databases
eggNOGi | KOG2046, Eukaryota |
GeneTreei | ENSGT00940000159680 |
HOGENOMi | CLU_055232_0_0_1 |
InParanoidi | Q08091 |
PhylomeDBi | Q08091 |
TreeFami | TF313921 |
Miscellaneous databases
BioGRID-ORCSi | 12797, 1 hit in 18 CRISPR screens |
PROi | PR:Q08091 |
RNActi | Q08091, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000001349, Expressed in urinary bladder and 161 other tissues |
ExpressionAtlasi | Q08091, baseline and differential |
Genevisiblei | Q08091, MM |
Family and domain databases
CDDi | cd00014, CH, 1 hit |
Gene3Di | 1.10.418.10, 1 hit |
InterProi | View protein in InterPro IPR001997, Calponin/LIMCH1 IPR000557, Calponin_repeat IPR001715, CH-domain IPR036872, CH_dom_sf IPR003096, SM22_calponin |
Pfami | View protein in Pfam PF00402, Calponin, 3 hits PF00307, CH, 1 hit |
PRINTSi | PR00889, CALPONIN PR00888, SM22CALPONIN |
SMARTi | View protein in SMART SM00033, CH, 1 hit |
SUPFAMi | SSF47576, SSF47576, 1 hit |
PROSITEi | View protein in PROSITE PS01052, CALPONIN_1, 3 hits PS51122, CALPONIN_2, 3 hits PS50021, CH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CNN1_MOUSE | |
Accessioni | Q08091Primary (citable) accession number: Q08091 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families