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Protein

Hepatocyte growth factor

Gene

Hgf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.1 Publication

Caution

Has lost two of the three essential catalytic residues and so probably has no enzymatic activity.Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGrowth factor, Serine protease homolog

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-1257604 PIP3 activates AKT signaling
R-MMU-5673001 RAF/MAP kinase cascade
R-MMU-6806942 MET Receptor Activation
R-MMU-6807004 Negative regulation of MET activity
R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-MMU-8851805 MET activates RAS signaling
R-MMU-8851907 MET activates PI3K/AKT signaling
R-MMU-8865999 MET activates PTPN11
R-MMU-8874081 MET activates PTK2 signaling
R-MMU-8875513 MET interacts with TNS proteins
R-MMU-8875555 MET activates RAP1 and RAC1
R-MMU-8875656 MET receptor recycling
R-MMU-8875791 MET activates STAT3
R-MMU-9032759 NTRK2 activates RAC1

Protein family/group databases

MEROPSiS01.982

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte growth factor
Alternative name(s):
Hepatopoietin-A
Scatter factor
Short name:
SF
Cleaved into the following 2 chains:
Gene namesi
Name:Hgf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:96079 Hgf

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32By similarityAdd BLAST32
ChainiPRO_000002809333 – 495Hepatocyte growth factor alpha chainAdd BLAST463
ChainiPRO_0000028094496 – 728Hepatocyte growth factor beta chainAdd BLAST233

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi71 ↔ 97
Disulfide bondi75 ↔ 85
Disulfide bondi129 ↔ 207
Disulfide bondi150 ↔ 190
Disulfide bondi178 ↔ 202
Disulfide bondi212 ↔ 289By similarity
Disulfide bondi233 ↔ 272By similarity
Disulfide bondi261 ↔ 284By similarity
Glycosylationi295N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi306 ↔ 384By similarity
Disulfide bondi327 ↔ 366By similarity
Disulfide bondi355 ↔ 378By similarity
Disulfide bondi392 ↔ 470By similarity
Glycosylationi403N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi413 ↔ 453By similarity
Disulfide bondi441 ↔ 465By similarity
Disulfide bondi488 ↔ 607Interchain (between alpha and beta chains)PROSITE-ProRule annotation
Disulfide bondi520 ↔ 536By similarity
Glycosylationi569N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi615 ↔ 682By similarity
Disulfide bondi645 ↔ 661By similarity
Glycosylationi656N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi672 ↔ 700By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiQ08048
PRIDEiQ08048

PTM databases

iPTMnetiQ08048
PhosphoSitePlusiQ08048

Expressioni

Gene expression databases

BgeeiENSMUSG00000028864
CleanExiMM_HGF
GenevisibleiQ08048 MM

Interactioni

Subunit structurei

Dimer of an alpha chain and a beta chain linked by a disulfide bond. Interacts with SRPX2; the interaction increases HGF mitogenic activity.

Binary interactionsi

WithEntry#Exp.IntActNotes
METP085813EBI-15655650,EBI-1039152From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-46456N
IntActiQ08048, 1 interactor
STRINGi10090.ENSMUSP00000030683

Structurei

Secondary structure

1728
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi40 – 42Combined sources3
Beta strandi43 – 52Combined sources10
Beta strandi55 – 57Combined sources3
Beta strandi61 – 64Combined sources4
Helixi68 – 77Combined sources10
Turni78 – 80Combined sources3
Beta strandi87 – 91Combined sources5
Turni92 – 95Combined sources4
Beta strandi96 – 101Combined sources6
Beta strandi106 – 108Combined sources3
Beta strandi110 – 122Combined sources13
Helixi123 – 125Combined sources3
Beta strandi129 – 133Combined sources5
Beta strandi149 – 151Combined sources3
Beta strandi157 – 159Combined sources3
Turni165 – 167Combined sources3
Beta strandi188 – 194Combined sources7
Beta strandi198 – 201Combined sources4
Turni207 – 209Combined sources3
Beta strandi240 – 242Combined sources3
Helixi248 – 250Combined sources3
Turni252 – 255Combined sources4
Beta strandi271 – 275Combined sources5
Beta strandi280 – 283Combined sources4

3D structure databases

ProteinModelPortaliQ08048
SMRiQ08048
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ08048

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 124PANPROSITE-ProRule annotationAdd BLAST87
Domaini129 – 207Kringle 1PROSITE-ProRule annotationAdd BLAST79
Domaini212 – 289Kringle 2PROSITE-ProRule annotationAdd BLAST78
Domaini306 – 384Kringle 3PROSITE-ProRule annotationAdd BLAST79
Domaini392 – 470Kringle 4PROSITE-ProRule annotationAdd BLAST79
Domaini496 – 724Peptidase S1PROSITE-ProRule annotationAdd BLAST229

Sequence similaritiesi

Belongs to the peptidase S1 family. Plasminogen subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Kringle, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IDXR Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000119133
HOGENOMiHOG000112892
HOVERGENiHBG004381
InParanoidiQ08048
KOiK05460
OMAiPWDYCPI
OrthoDBiEOG091G0AH5
PhylomeDBiQ08048
TreeFamiTF329901

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
Gene3Di2.40.20.10, 4 hits
InterProiView protein in InterPro
IPR027284 Hepatocyte_GF
IPR024174 HGF-like
IPR000001 Kringle
IPR013806 Kringle-like
IPR018056 Kringle_CS
IPR038178 Kringle_sf
IPR003609 Pan_app
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
PfamiView protein in Pfam
PF00051 Kringle, 4 hits
PF00024 PAN_1, 1 hit
PF00089 Trypsin, 1 hit
PIRSFiPIRSF500183 Hepatocyte_GF, 1 hit
PIRSF001152 HGF_MST1, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00130 KR, 4 hits
SM00473 PAN_AP, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF57440 SSF57440, 4 hits
PROSITEiView protein in PROSITE
PS00021 KRINGLE_1, 4 hits
PS50070 KRINGLE_2, 4 hits
PS50948 PAN, 1 hit
PS50240 TRYPSIN_DOM, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q08048-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMWGTKLLPV LLLQHVLLHL LLLHVAIPYA EGQKKRRNTL HEFKKSAKTT
60 70 80 90 100
LTKEDPLLKI KTKKVNSADE CANRCIRNRG FTFTCKAFVF DKSRKRCYWY
110 120 130 140 150
PFNSMSSGVK KGFGHEFDLY ENKDYIRNCI IGKGGSYKGT VSITKSGIKC
160 170 180 190 200
QPWNSMIPHE HSFLPSSYRG KDLQENYCRN PRGEEGGPWC FTSNPEVRYE
210 220 230 240 250
VCDIPQCSEV ECMTCNGESY RGPMDHTESG KTCQRWDQQT PHRHKFLPER
260 270 280 290 300
YPDKGFDDNY CRNPDGKPRP WCYTLDPDTP WEYCAIKTCA HSAVNETDVP
310 320 330 340 350
METTECIQGQ GEGYRGTSNT IWNGIPCQRW DSQYPHKHDI TPENFKCKDL
360 370 380 390 400
RENYCRNPDG AESPWCFTTD PNIRVGYCSQ IPKCDVSSGQ DCYRGNGKNY
410 420 430 440 450
MGNLSKTRSG LTCSMWDKNM EDLHRHIFWE PDASKLNKNY CRNPDDDAHG
460 470 480 490 500
PWCYTGNPLI PWDYCPISRC EGDTTPTIVN LDHPVISCAK TKQLRVVNGI
510 520 530 540 550
PTQTTVGWMV SLKYRNKHIC GGSLIKESWV LTARQCFPAR NKDLKDYEAW
560 570 580 590 600
LGIHDVHERG EEKRKQILNI SQLVYGPEGS DLVLLKLARP AILDNFVSTI
610 620 630 640 650
DLPSYGCTIP EKTTCSIYGW GYTGLINADG LLRVAHLYIM GNEKCSQHHQ
660 670 680 690 700
GKVTLNESEL CAGAEKIGSG PCEGDYGGPL ICEQHKMRMV LGVIVPGRGC
710 720
AIPNRPGIFV RVAYYAKWIH KVILTYKL
Length:728
Mass (Da):82,945
Last modified:November 1, 1995 - v1
Checksum:iA0381FC497534328
GO
Isoform Short (identifier: Q08048-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     163-167: Missing.

Show »
Length:723
Mass (Da):82,413
Checksum:i7BC19A16C399EDD2
GO
Isoform NK1 (identifier: Q08048-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     210-211: VE → GK
     212-728: Missing.

Show »
Length:211
Mass (Da):24,308
Checksum:i758BD0687A835F48
GO

Sequence cautioni

The sequence CAA51054 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti189W → K AA sequence (PubMed:2144630).Curated1
Sequence conflicti344N → K in AAB31855 (PubMed:8081873).Curated1
Sequence conflicti378C → E AA sequence (PubMed:2144630).Curated1
Sequence conflicti380Q → E AA sequence (PubMed:2144630).Curated1
Sequence conflicti479V → L in AAB31855 (PubMed:8081873).Curated1
Sequence conflicti513K → L AA sequence (PubMed:2142751).Curated1
Sequence conflicti518H → T AA sequence (PubMed:1831975).Curated1
Sequence conflicti564R → H in CAA51054 (PubMed:8241272).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005408163 – 167Missing in isoform Short. 1 Publication5
Alternative sequenceiVSP_044345210 – 211VE → GK in isoform NK1. 1 Publication2
Alternative sequenceiVSP_044346212 – 728Missing in isoform NK1. 1 PublicationAdd BLAST517

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10212 mRNA Translation: BAA01064.1
D10213 mRNA Translation: BAA01065.1
S71816 mRNA Translation: AAB31855.1
X72307 mRNA Translation: CAA51054.1 Different initiation.
AF042856 mRNA Translation: AAC40051.1
X84046 mRNA Translation: CAA58865.1
CH466586 Genomic DNA Translation: EDL03238.1
BC119228 mRNA Translation: AAI19229.1
X81630 Genomic DNA Translation: CAA57286.1
CCDSiCCDS19097.1 [Q08048-1]
CCDS80213.1 [Q08048-2]
PIRiJC2117 A60185
RefSeqiNP_001276387.1, NM_001289458.1 [Q08048-1]
NP_001276388.1, NM_001289459.1 [Q08048-1]
NP_001276389.1, NM_001289460.2 [Q08048-3]
NP_001276390.1, NM_001289461.1 [Q08048-2]
NP_034557.3, NM_010427.5 [Q08048-1]
UniGeneiMm.267078

Genome annotation databases

EnsembliENSMUST00000030683; ENSMUSP00000030683; ENSMUSG00000028864 [Q08048-1]
ENSMUST00000196645; ENSMUSP00000142517; ENSMUSG00000028864 [Q08048-2]
ENSMUST00000199581; ENSMUSP00000143424; ENSMUSG00000028864 [Q08048-1]
GeneIDi15234
KEGGimmu:15234
UCSCiuc008wne.2 mouse [Q08048-3]
uc008wnf.2 mouse [Q08048-1]
uc008wnj.1 mouse [Q08048-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHGF_MOUSE
AccessioniPrimary (citable) accession number: Q08048
Secondary accession number(s): O55027
, Q53WS5, Q61662, Q64007, Q6LBE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 20, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

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