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Protein

Core-binding factor subunit beta

Gene

Cbfb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation (PubMed:18258917).3 Publications1 Publication

GO - Molecular functioni

  • DNA binding Source: MGI
  • transcription coactivator activity Source: GO_Central

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-8877330 RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs)
R-MMU-8878166 Transcriptional regulation by RUNX2
R-MMU-8931987 RUNX1 regulates estrogen receptor mediated transcription
R-MMU-8934593 Regulation of RUNX1 Expression and Activity
R-MMU-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-MMU-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-MMU-8939243 RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known
R-MMU-8939245 RUNX1 regulates transcription of genes involved in BCR signaling
R-MMU-8939246 RUNX1 regulates transcription of genes involved in differentiation of myeloid cells
R-MMU-8939247 RUNX1 regulates transcription of genes involved in interleukin signaling
R-MMU-8941326 RUNX2 regulates bone development
R-MMU-8941858 Regulation of RUNX3 expression and activity
R-MMU-8951936 RUNX3 regulates p14-ARF
R-MMU-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Core-binding factor subunit beta
Short name:
CBF-beta
Alternative name(s):
Polyomavirus enhancer-binding protein 2 beta subunit
Short name:
PEA2-beta
Short name:
PEBP2-beta
SL3-3 enhancer factor 1 subunit beta
SL3/AKV core-binding factor beta subunit
Gene namesi
Name:Cbfb
Synonyms:Pebp2b, Pebpb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:99851 Cbfb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi5V → A: Interferes with heterodimerization. 1 Publication1
Mutagenesisi63N → A: Interferes with heterodimerization. 1 Publication1
Mutagenesisi104N → A: Interferes with heterodimerization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000583021 – 187Core-binding factor subunit betaAdd BLAST187

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10Phosphoserine; by CK2Sequence analysis1
Modified residuei159Phosphoserine; by PKCSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ08024
MaxQBiQ08024
PaxDbiQ08024
PeptideAtlasiQ08024
PRIDEiQ08024

PTM databases

iPTMnetiQ08024
PhosphoSitePlusiQ08024

Expressioni

Tissue specificityi

Expressed in all tissues tested. Highest level in thymus, but also abundantly expressed in muscle, lung and brain.

Gene expression databases

BgeeiENSMUSG00000031885 Expressed in 317 organ(s), highest expression level in trigeminal ganglion
CleanExiMM_CBFB
GenevisibleiQ08024 MM

Interactioni

Subunit structurei

Heterodimer with RUNX1, RUNX2 and RUNX3 (Probable). Interacts with COPRS. Found in a complex with PRMT5 and RUNX1 (PubMed:22193545).1 Publication1 Publication

Protein-protein interaction databases

BioGridi198525, 7 interactors
IntActiQ08024, 3 interactors
STRINGi10090.ENSMUSP00000059382

Structurei

Secondary structure

1187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ08024
SMRiQ08024
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ08024

Family & Domainsi

Sequence similaritiesi

Belongs to the CBF-beta family.Curated

Phylogenomic databases

eggNOGiKOG4785 Eukaryota
ENOG410Z04Q LUCA
GeneTreeiENSGT00390000018132
HOGENOMiHOG000008040
HOVERGENiHBG000541
InParanoidiQ08024
OMAiPGKVHIK
OrthoDBiEOG091G0VFL
PhylomeDBiQ08024
TreeFamiTF314675

Family and domain databases

Gene3Di2.40.250.10, 1 hit
InterProiView protein in InterPro
IPR003417 CBF_beta
IPR036552 CBF_bsu_sf
PANTHERiPTHR10276 PTHR10276, 1 hit
PfamiView protein in Pfam
PF02312 CBF_beta, 1 hit
SUPFAMiSSF50723 SSF50723, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q08024-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPRVVPDQRS KFENEEFFRK LSRECEIKYT GFRDRPHEER QTRFQNACRD
60 70 80 90 100
GRSEIAFVAT GTNLSLQFFP ASWQGEQRQT PSREYVDLER EAGKVYLKAP
110 120 130 140 150
MILNGVCVIW KGWIDLHRLD GMGCLEFDEE RAQQEDALAQ QAFEEARRRT
160 170 180
REFEDRDRSH REEMEARRQQ DPSPGSNLGG GDDLKLR
Length:187
Mass (Da):22,030
Last modified:November 1, 1996 - v1
Checksum:i0B2E6101A35D0FD8
GO
Isoform 2 (identifier: Q08024-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     166-187: ARRQQDPSPGSNLGGGDDLKLR → VRVSQLLAVTGKKTARP

Note: Major isoform.
Show »
Length:182
Mass (Da):21,517
Checksum:i20EC3C81F39874FA
GO
Isoform 3 (identifier: Q08024-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     134-165: Missing.

Note: Does not dimerize with the alpha subunit.
Show »
Length:155
Mass (Da):18,026
Checksum:i1E4C0F13B9B25697
GO
Isoform 4 (identifier: Q08024-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-94: Missing.

Note: Does not dimerize with the alpha subunit.
Show »
Length:148
Mass (Da):17,633
Checksum:i4265D3ADB61D1352
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2P → S in BAC40748 (PubMed:16141072).Curated1
Sequence conflicti40R → P in BAC40748 (PubMed:16141072).Curated1
Sequence conflicti171D → N in L03279 (PubMed:8497254).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00435856 – 94Missing in isoform 4. 1 PublicationAdd BLAST39
Alternative sequenceiVSP_004359134 – 165Missing in isoform 3. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_004360166 – 187ARRQQ…DLKLR → VRVSQLLAVTGKKTARP in isoform 2. 2 PublicationsAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14572 mRNA Translation: BAA03426.1
D14571 mRNA Translation: BAA03425.1
D14570 mRNA Translation: BAA03424.1
D14569 Genomic DNA Translation: BAA03423.1
L03305 mRNA No translation available.
L03306 mRNA No translation available.
L03279 mRNA No translation available.
AK089094 mRNA Translation: BAC40748.1
BC006763 mRNA Translation: AAH06763.1
BC026749 mRNA Translation: AAH26749.1
BC040752 mRNA Translation: AAH40752.2
CCDSiCCDS22592.1 [Q08024-1]
CCDS52654.1 [Q08024-2]
CCDS52655.1 [Q08024-3]
CCDS52656.1 [Q08024-4]
PIRiA46107
B48124
RefSeqiNP_001154928.1, NM_001161456.1 [Q08024-4]
NP_001154929.1, NM_001161457.1 [Q08024-3]
NP_001154930.1, NM_001161458.1 [Q08024-2]
NP_071704.3, NM_022309.4 [Q08024-1]
UniGeneiMm.2018

Genome annotation databases

EnsembliENSMUST00000052209; ENSMUSP00000059382; ENSMUSG00000031885 [Q08024-1]
ENSMUST00000109392; ENSMUSP00000105019; ENSMUSG00000031885 [Q08024-2]
ENSMUST00000109394; ENSMUSP00000105021; ENSMUSG00000031885 [Q08024-4]
ENSMUST00000109395; ENSMUSP00000105022; ENSMUSG00000031885 [Q08024-3]
GeneIDi12400
KEGGimmu:12400
UCSCiuc009nbp.2 mouse [Q08024-1]
uc009nbq.2 mouse [Q08024-2]
uc009nbr.2 mouse [Q08024-4]
uc012gjd.1 mouse [Q08024-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14572 mRNA Translation: BAA03426.1
D14571 mRNA Translation: BAA03425.1
D14570 mRNA Translation: BAA03424.1
D14569 Genomic DNA Translation: BAA03423.1
L03305 mRNA No translation available.
L03306 mRNA No translation available.
L03279 mRNA No translation available.
AK089094 mRNA Translation: BAC40748.1
BC006763 mRNA Translation: AAH06763.1
BC026749 mRNA Translation: AAH26749.1
BC040752 mRNA Translation: AAH40752.2
CCDSiCCDS22592.1 [Q08024-1]
CCDS52654.1 [Q08024-2]
CCDS52655.1 [Q08024-3]
CCDS52656.1 [Q08024-4]
PIRiA46107
B48124
RefSeqiNP_001154928.1, NM_001161456.1 [Q08024-4]
NP_001154929.1, NM_001161457.1 [Q08024-3]
NP_001154930.1, NM_001161458.1 [Q08024-2]
NP_071704.3, NM_022309.4 [Q08024-1]
UniGeneiMm.2018

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ILFNMR-A1-141[»]
1IO4X-ray3.00D1-141[»]
2JHBNMR-A1-141[»]
3WTSX-ray2.35B/G1-142[»]
3WTTX-ray2.35B/G1-142[»]
3WTUX-ray2.70B/G1-142[»]
3WTVX-ray2.70B/G1-142[»]
3WTWX-ray2.90B/G1-142[»]
3WTXX-ray2.80B/G1-142[»]
3WTYX-ray2.70B/G1-142[»]
ProteinModelPortaliQ08024
SMRiQ08024
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198525, 7 interactors
IntActiQ08024, 3 interactors
STRINGi10090.ENSMUSP00000059382

PTM databases

iPTMnetiQ08024
PhosphoSitePlusiQ08024

Proteomic databases

EPDiQ08024
MaxQBiQ08024
PaxDbiQ08024
PeptideAtlasiQ08024
PRIDEiQ08024

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052209; ENSMUSP00000059382; ENSMUSG00000031885 [Q08024-1]
ENSMUST00000109392; ENSMUSP00000105019; ENSMUSG00000031885 [Q08024-2]
ENSMUST00000109394; ENSMUSP00000105021; ENSMUSG00000031885 [Q08024-4]
ENSMUST00000109395; ENSMUSP00000105022; ENSMUSG00000031885 [Q08024-3]
GeneIDi12400
KEGGimmu:12400
UCSCiuc009nbp.2 mouse [Q08024-1]
uc009nbq.2 mouse [Q08024-2]
uc009nbr.2 mouse [Q08024-4]
uc012gjd.1 mouse [Q08024-3]

Organism-specific databases

CTDi865
MGIiMGI:99851 Cbfb

Phylogenomic databases

eggNOGiKOG4785 Eukaryota
ENOG410Z04Q LUCA
GeneTreeiENSGT00390000018132
HOGENOMiHOG000008040
HOVERGENiHBG000541
InParanoidiQ08024
OMAiPGKVHIK
OrthoDBiEOG091G0VFL
PhylomeDBiQ08024
TreeFamiTF314675

Enzyme and pathway databases

ReactomeiR-MMU-8877330 RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs)
R-MMU-8878166 Transcriptional regulation by RUNX2
R-MMU-8931987 RUNX1 regulates estrogen receptor mediated transcription
R-MMU-8934593 Regulation of RUNX1 Expression and Activity
R-MMU-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-MMU-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-MMU-8939243 RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known
R-MMU-8939245 RUNX1 regulates transcription of genes involved in BCR signaling
R-MMU-8939246 RUNX1 regulates transcription of genes involved in differentiation of myeloid cells
R-MMU-8939247 RUNX1 regulates transcription of genes involved in interleukin signaling
R-MMU-8941326 RUNX2 regulates bone development
R-MMU-8941858 Regulation of RUNX3 expression and activity
R-MMU-8951936 RUNX3 regulates p14-ARF
R-MMU-9018519 Estrogen-dependent gene expression

Miscellaneous databases

ChiTaRSiCbfb mouse
EvolutionaryTraceiQ08024
PROiPR:Q08024
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031885 Expressed in 317 organ(s), highest expression level in trigeminal ganglion
CleanExiMM_CBFB
GenevisibleiQ08024 MM

Family and domain databases

Gene3Di2.40.250.10, 1 hit
InterProiView protein in InterPro
IPR003417 CBF_beta
IPR036552 CBF_bsu_sf
PANTHERiPTHR10276 PTHR10276, 1 hit
PfamiView protein in Pfam
PF02312 CBF_beta, 1 hit
SUPFAMiSSF50723 SSF50723, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPEBB_MOUSE
AccessioniPrimary (citable) accession number: Q08024
Secondary accession number(s): Q08025
, Q62050, Q62051, Q8C282, Q8CGD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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