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Protein

Mitochondrial import inner membrane translocase subunit TIM14

Gene

PAM18

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to stimulate activity of mtHSP70 (SSC1).3 Publications

GO - Molecular functioni

  • ATPase activator activity Source: SGD

GO - Biological processi

  • protein import into mitochondrial matrix Source: SGD

Keywordsi

Molecular functionChaperone
Biological processProtein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32169-MONOMER

Protein family/group databases

TCDBi3.A.8.1.1 the mitochondrial protein translocase (mpt) family

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM14
Alternative name(s):
Presequence translocated-associated motor subunit PAM18
Gene namesi
Name:PAM18
Synonyms:TIM14
Ordered Locus Names:YLR008C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR008C
SGDiS000003998 PAM18

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 65Mitochondrial intermembraneSequence analysisAdd BLAST65
Transmembranei66 – 83HelicalSequence analysisAdd BLAST18
Topological domaini84 – 168Mitochondrial matrixSequence analysisAdd BLAST85

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi141 – 143HPD → AAA: Induces lethality. 1 Publication3
Mutagenesisi141H → Q: Induces lethality. 2 Publications1
Mutagenesisi142P → A: Induces a strong reduction in ability to stimulate mtHSP70 activity but does not affect import of proteins into mitochondrial matrix. 1 Publication1
Mutagenesisi143D → N: Induces lethality. 1 Publication1
Mutagenesisi144K → Q: Induces a strong reduction in ability to stimulate mtHSP70 activity but does not affect import of proteins into mitochondrial matrix. 1 Publication1
Mutagenesisi150L → W: Temperature-sensitive mutant that impairs heterodimerization with PAM16 and import of proteins into mitochondrial matrix when incubated at 37 degrees Celsius. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000711181 – 168Mitochondrial import inner membrane translocase subunit TIM14Add BLAST168

Proteomic databases

MaxQBiQ07914
PaxDbiQ07914
PRIDEiQ07914

Interactioni

Subunit structurei

Homodimer and heterodimer with PAM16/TIM16. Homodimerization may not be relevant in vivo, while heterodimerization is essential for activity regulation of mtHSP70. Component of the PAM complex, at least composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18/TIM14. Interacts directly with mtHsp70. Interacts directly with TIM17 subunit of the TIM23 complex.6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31281, 202 interactors
ComplexPortaliCPX-539 Mitochondrial inner membrane pre-sequence translocase complex
DIPiDIP-2125N
IntActiQ07914, 30 interactors
MINTiQ07914
STRINGi4932.YLR008C

Structurei

Secondary structure

1168
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi110 – 116Combined sources7
Turni121 – 123Combined sources3
Helixi126 – 140Combined sources15
Helixi142 – 144Combined sources3
Helixi148 – 164Combined sources17

3D structure databases

ProteinModelPortaliQ07914
SMRiQ07914
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ07914

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini112 – 168JPROSITE-ProRule annotationAdd BLAST57

Domaini

The J domain is essential for co-chaperone activity and mediates the heterodimerization with the J-like domain of PAM16.1 Publication

Sequence similaritiesi

Belongs to the TIM14 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000017303
HOGENOMiHOG000115841
InParanoidiQ07914
KOiK09539
OMAiMANHPDK
OrthoDBiEOG092C5SDV

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR036869 J_dom_sf
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
PROSITEiView protein in PROSITE
PS50076 DNAJ_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q07914-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSQSNTGNS IEAPQLPIPG QTNGSANVTV DGAGVNVGIQ NGSQGQKTGM
60 70 80 90 100
DLYFDQALNY MGEHPVITGF GAFLTLYFTA GAYKSISKGL NGGKSTTAFL
110 120 130 140 150
KGGFDPKMNS KEALQILNLT ENTLTKKKLK EVHRKIMLAN HPDKGGSPFL
160
ATKINEAKDF LEKRGISK
Length:168
Mass (Da):17,910
Last modified:November 1, 1996 - v1
Checksum:i9CBF71CA50A0F341
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73180 Genomic DNA Translation: CAA97530.1
AY558196 Genomic DNA Translation: AAS56522.1
BK006945 Genomic DNA Translation: DAA09326.1
PIRiS64830
RefSeqiNP_013108.1, NM_001181895.1

Genome annotation databases

EnsemblFungiiYLR008C; YLR008C; YLR008C
GeneIDi850694
KEGGisce:YLR008C

Similar proteinsi

Entry informationi

Entry nameiTIM14_YEAST
AccessioniPrimary (citable) accession number: Q07914
Secondary accession number(s): D6VY10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 1, 1996
Last modified: July 18, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

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