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UniProtKB - Q07868 (PBP2B_BACSU)
Protein
Penicillin-binding protein 2B
Gene
pbpB
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active site of these proteins, thereby interrupting normal cell wall synthesis (Probable). This protein itself, but not its transpeptidase activity, is required for cell division (PubMed:28792086).
Curated1 PublicationCatalytic activityi
- Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.1 Publication EC:3.4.16.4
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 309 | Acyl-ester intermediate1 PublicationBy similarity | 1 |
GO - Molecular functioni
- penicillin binding Source: GO_Central
- serine-type D-Ala-D-Ala carboxypeptidase activity Source: UniProtKB-EC
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cell wall organization Source: GO_Central
- peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
- regulation of cell shape Source: UniProtKB-KW
- response to antibiotic Source: UniProtKB-KW
- sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Protease |
Biological process | Antibiotic resistance, Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis, Sporulation |
Enzyme and pathway databases
BioCyci | BSUB:BSU15160-MONOMER |
UniPathwayi | UPA00219 |
Names & Taxonomyi
Protein namesi | Recommended name: Penicillin-binding protein 2B1 Publication (EC:3.4.16.41 Publication)Short name: PBP-2B Alternative name(s): Penicillin-binding protein B Penicillin-sensitive peptidoglycan D,D-transpeptidaseCurated |
Gene namesi | Name:pbpB1 Publication Ordered Locus Names:BSU15160 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication2 Publications; Single-pass membrane protein Sequence analysis
Note: Localizes at mid cell, to cell division sites; localization requires FtsZ.1 Publication
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 10 | Cytoplasmic1 Publication | 10 | |
Transmembranei | 11 – 28 | Helical1 PublicationSequence analysisAdd BLAST | 18 | |
Topological domaini | 29 – 309 | Extracellular1 PublicationAdd BLAST | 281 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Essential, it cannot be deleted (PubMed:8244929, PubMed:8636036). Depletion leads to an arrest of cell division after 80 minutes at 37 degrees Celsius and 40 minutes at 48 degrees Celsius with a concomitant reduction in FtsL and to a lesser extent DivIC levels; cells lengthen after arrest and eventually lyse (PubMed:16936019, PubMed:28792086).4 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 309 | S → A: Bacteria grow as well as wild-type, are slightly longer, protein no longer binds bocillin-FL (a penicillin analog). Increased sensitivity to cefoxitin, very slight increase in resistance to penicillin G and cephalexin. 1 Publication | 1 | |
Mutagenesisi | 630 – 713 | AVKEQ…FKNPD → SFCSL: Cells are viable and motile, however they are filamentous and only about 20% of cells produce spores. Less protein is made, still binds penicillin. 1 PublicationAdd BLAST | 84 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000195463 | 1 – 713 | Penicillin-binding protein 2BAdd BLAST | 713 |
Proteomic databases
PaxDbi | Q07868 |
PRIDEi | Q07868 |
Expressioni
Developmental stagei
Synthesized throughout vegetative growth. Synthesis is enhanced during stage II of sporulation and in stage IV mother cells. Undetectable in stage IV forespores. Present in the inner forespore membrane of the dormant spore.1 Publication
Inductioni
Transcribed at a low constant level in all growth phases. Part of the mraZ-rsmH-ftsL-pbpB operon.1 Publication
Interactioni
Binary interactionsi
Q07868
With | #Exp. | IntAct |
---|---|---|
divIB [P16655] | 3 | EBI-5243272,EBI-5243256 |
Protein-protein interaction databases
IntActi | Q07868, 14 interactors |
STRINGi | 224308.BSU15160 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 595 – 654 | PASTA 1PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 655 – 711 | PASTA 2PROSITE-ProRule annotationAdd BLAST | 57 |
Sequence similaritiesi
Belongs to the transpeptidase family.Curated
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0768, Bacteria |
InParanoidi | Q07868 |
PhylomeDBi | Q07868 |
Family and domain databases
Gene3Di | 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR005543, PASTA_dom IPR005311, PBP_dimer IPR036138, PBP_dimer_sf IPR001460, PCN-bd_Tpept |
Pfami | View protein in Pfam PF03793, PASTA, 2 hits PF03717, PBP_dimer, 1 hit PF00905, Transpeptidase, 1 hit |
SMARTi | View protein in SMART SM00740, PASTA, 2 hits |
SUPFAMi | SSF56519, SSF56519, 1 hit SSF56601, SSF56601, 1 hit |
PROSITEi | View protein in PROSITE PS51178, PASTA, 2 hits |
i Sequence
Sequence statusi: Complete.
Q07868-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPKKNKFMNR GAAILSICFA LFFFVILGRM AYIQITGKAN GEVLATKATE
60 70 80 90 100
QHEKKRTIEA SRGSILDRKG KVIAEDTATY KLIAILDKKM TTDVKHPQHV
110 120 130 140 150
VNKEKTAEAL SKVINLDKAD ILDILNKDAK QVEFGSAGRD ITYSQKQKIE
160 170 180 190 200
KMKLPGISFL RDTKRYYPNG VFASNLIGYA EVDEETNEIS GAMGLEKVLD
210 220 230 240 250
KYLKERDGYV TYESDKSGWE LPNSKNKITA PKNGDNVYLT IDQKIQTFLE
260 270 280 290 300
DSMTKVAQKY NPKKIMAAVV DPKTGKVLAM GQRPSFDPNK RDVTNYYNDL
310 320 330 340 350
ISYAYEPGST MKIFTLAAAM QENVFNANEK YKSGTFEVGG APVKDHNNGV
360 370 380 390 400
GWGPTTYHDG VLRSSNVAFA KLAKEKLGYD RLNQYLHKFN FYQKTGIDLP
410 420 430 440 450
GEVSSKINFK YEFDKASTAY GQASAVTPIQ QIQAATAIAN DGKMMKPYVI
460 470 480 490 500
DHIVDPDKDK TIYQNKPESA GTPISASTAK KVRDILGEVV TSKIGTGQAY
510 520 530 540 550
KIEGFDVAGK TGTAQIAGKG GYLDGTDNYI FSFMGMAPKD DPELLIYVAV
560 570 580 590 600
QQPQLKAGQS SSDPVSEIFN PTMKNSLHYL NIEPTEKSDS DKEETKAQTM
610 620 630 640 650
PDLTDQTVAA AQKKAKEENL TPIVIGSDVA VKEQYPKADE EVLTNQKVFL
660 670 680 690 700
KTGGKIKMPD MTGWSRREVL QYGELAGIHI EVSGQGYAVS QSVKKDKEIK
710
DKTVIKVKFK NPD
Sequence cautioni
The sequence AAC36837 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAA81084 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAA92527 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAB13389 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 147 | Q → L in AAC36837 (PubMed:8244929).Curated | 1 | |
Sequence conflicti | 147 | Q → L in CAA92527 (PubMed:8636036).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L09703 Unassigned DNA Translation: AAC36837.1 Different initiation. Z68230 Genomic DNA Translation: CAA92527.1 Different initiation. AL009126 Genomic DNA Translation: CAB13389.2 Different initiation. Z25865 Genomic DNA Translation: CAA81084.1 Different initiation. |
PIRi | C53292 |
RefSeqi | NP_389399.2, NC_000964.3 |
Genome annotation databases
EnsemblBacteriai | CAB13389; CAB13389; BSU_15160 |
GeneIDi | 939847 |
KEGGi | bsu:BSU15160 |
PATRICi | fig|224308.43.peg.1610 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L09703 Unassigned DNA Translation: AAC36837.1 Different initiation. Z68230 Genomic DNA Translation: CAA92527.1 Different initiation. AL009126 Genomic DNA Translation: CAB13389.2 Different initiation. Z25865 Genomic DNA Translation: CAA81084.1 Different initiation. |
PIRi | C53292 |
RefSeqi | NP_389399.2, NC_000964.3 |
3D structure databases
AlphaFoldDBi | Q07868 |
SMRi | Q07868 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q07868, 14 interactors |
STRINGi | 224308.BSU15160 |
Proteomic databases
PaxDbi | Q07868 |
PRIDEi | Q07868 |
Genome annotation databases
EnsemblBacteriai | CAB13389; CAB13389; BSU_15160 |
GeneIDi | 939847 |
KEGGi | bsu:BSU15160 |
PATRICi | fig|224308.43.peg.1610 |
Phylogenomic databases
eggNOGi | COG0768, Bacteria |
InParanoidi | Q07868 |
PhylomeDBi | Q07868 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | BSUB:BSU15160-MONOMER |
Family and domain databases
Gene3Di | 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR005543, PASTA_dom IPR005311, PBP_dimer IPR036138, PBP_dimer_sf IPR001460, PCN-bd_Tpept |
Pfami | View protein in Pfam PF03793, PASTA, 2 hits PF03717, PBP_dimer, 1 hit PF00905, Transpeptidase, 1 hit |
SMARTi | View protein in SMART SM00740, PASTA, 2 hits |
SUPFAMi | SSF56519, SSF56519, 1 hit SSF56601, SSF56601, 1 hit |
PROSITEi | View protein in PROSITE PS51178, PASTA, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PBP2B_BACSU | |
Accessioni | Q07868Primary (citable) accession number: Q07868 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | August 12, 2020 | |
Last modified: | May 25, 2022 | |
This is version 148 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families