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Entry version 150 (05 Jun 2019)
Sequence version 1 (01 Nov 1996)
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Protein

Polyamine transporter 1

Gene

TPO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Catalyzes polyamine uptake at alkaline pH and excretion at acidic pH. Recognizes spermidine, spermine and putrescine, the polyamine analogs methylglyoxal bis(guanylhydrazone) (MGBG) and paraquat, the antimalarial drug quinidine, and cycloheximide. Confers resistance to the non-steroidal anti-inflammatory drug indomethacin.5 Publications

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processAntiport, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-32132-MONOMER

Protein family/group databases

Transport Classification Database

More...
TCDBi
2.A.1.2.16 the major facilitator superfamily (mfs)

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Polyamine transporter 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:TPO1
Ordered Locus Names:YLL028W
ORF Names:L0939
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XII

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YLL028W

Saccharomyces Genome Database

More...
SGDi
S000003951 TPO1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 145CytoplasmicSequence analysisAdd BLAST145
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Topological domaini167 – 181ExtracellularSequence analysisAdd BLAST15
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
Topological domaini203 – 212CytoplasmicSequence analysis10
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
Topological domaini234 – 242ExtracellularSequence analysis9
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 271CytoplasmicSequence analysis8
Transmembranei272 – 292HelicalSequence analysisAdd BLAST21
Topological domaini293 – 301ExtracellularSequence analysis9
Transmembranei302 – 322HelicalSequence analysisAdd BLAST21
Topological domaini323 – 376CytoplasmicSequence analysisAdd BLAST54
Transmembranei377 – 397HelicalSequence analysisAdd BLAST21
Topological domaini398 – 414ExtracellularSequence analysisAdd BLAST17
Transmembranei415 – 435HelicalSequence analysisAdd BLAST21
Topological domaini436 – 454CytoplasmicSequence analysisAdd BLAST19
Transmembranei455 – 475HelicalSequence analysisAdd BLAST21
Topological domaini476 – 483ExtracellularSequence analysis8
Transmembranei484 – 504HelicalSequence analysisAdd BLAST21
Topological domaini505CytoplasmicSequence analysis1
Transmembranei506 – 526HelicalSequence analysisAdd BLAST21
Topological domaini527 – 528ExtracellularSequence analysis2
Transmembranei529 – 549HelicalSequence analysisAdd BLAST21
Transmembranei550 – 570HelicalSequence analysisAdd BLAST21
Topological domaini571 – 586CytoplasmicSequence analysisAdd BLAST16

Keywords - Cellular componenti

Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi19S → A: Reduces transport activity. 1 Publication1
Mutagenesisi19S → E: Enhances transport activity. 1 Publication1
Mutagenesisi52T → A: Reduces transport activity. 1 Publication1
Mutagenesisi52T → E: Enhances transport activity. 1 Publication1
Mutagenesisi207E → Q: Loss of function. 1 Publication1
Mutagenesisi323E → Q: Loss of function; when associated with Q-324. 1 Publication1
Mutagenesisi324E → Q: Loss of function; when associated with Q-323. 1 Publication1
Mutagenesisi342S → A: Interferes with correct plasma membrane localization. 1 Publication1
Mutagenesisi342S → E: Enhances transport activity. 1 Publication1
Mutagenesisi574E → Q: Loss of function. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002627301 – 586Polyamine transporter 1Add BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei19Phosphoserine; by PKC1 Publication1
Modified residuei52Phosphothreonine; by CK11 Publication1
Modified residuei72PhosphoserineCombined sources1
Modified residuei76PhosphoserineCombined sources1
Modified residuei89PhosphothreonineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Modified residuei342Phosphoserine; by PKA1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q07824

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q07824

PRoteomics IDEntifications database

More...
PRIDEi
Q07824

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q07824

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

By transcription factor PDR1, by transcription factor HAA1 in response to acetaldehyde accumulation and by the non-steroidal anti-inflammatory drug indomethacin.3 Publications

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
31224, 138 interactors

Database of interacting proteins

More...
DIPi
DIP-1706N

Protein interaction database and analysis system

More...
IntActi
Q07824, 29 interactors

Molecular INTeraction database

More...
MINTi
Q07824

STRING: functional protein association networks

More...
STRINGi
4932.YLL028W

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi17 – 25Poly-Ser9

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000160689

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q07824

KEGG Orthology (KO)

More...
KOi
K08157

Identification of Orthologs from Complete Genome Data

More...
OMAi
LSIYMSF

Family and domain databases

Conserved Domains Database

More...
CDDi
cd06174 MFS, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF07690 MFS_1, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF103473 SSF103473, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50850 MFS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q07824-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDHSPISNK ENHLLPSDSS RSSSSDMHST GTTGTTGVEP VDFTGEGAKY
60 70 80 90 100
TTATEGNGGA DLAIQRTTTM NSAAESEVNI TRRLTKILTG SVNEPDRVEV
110 120 130 140 150
DYTNCAPMGG DRPYPPSLPS RDLYEVTFDG PNDPLHPFNW PMKKKVLLCL
160 170 180 190 200
VLCLDSIAIA MCSSIFASAV PQICEIYHVI EVVAILGITL FVLGFAASPV
210 220 230 240 250
IYAPLSELYG RKGVLVLSAF GFALFQFAVA TAENLQTIFI CRFFGGFIGA
260 270 280 290 300
APMAVVPAAF ADMFDTNVRG KAIALFSLGV FVGPILSPVM GSYIAQRTTW
310 320 330 340 350
RWLEYVVGCF ASAVFVAIVL FFEETHHPTI LVNKAKQMRK QSNNWGIHAA
360 370 380 390 400
HEDVELSIKD IVQKTVTRPI IMLFVEPLLL FVTIYNSFVY GILYLLLEAY
410 420 430 440 450
PLVFVEGYGF TENGELPYIA LIIGMMVCAA FIWYMDNDYL KRCRAKGKLV
460 470 480 490 500
PEARLYAMVI AGTVFPIGIL WFCWTGYYPH KIHWMVPTVG GAFIGFGLMG
510 520 530 540 550
IFLPCLNYII ESYLLLAASA VAANTFMRSA FGACFPLFAG YMFRGMGIGW
560 570 580
AGLLLGLFAA AMIPVPLLFL KYGESIRKKS KYAYAA
Length:586
Mass (Da):64,272
Last modified:November 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i32DBFB795617D036
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Z73133 Genomic DNA Translation: CAA97477.1
BK006945 Genomic DNA Translation: DAA09292.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S64779

NCBI Reference Sequences

More...
RefSeqi
NP_013072.1, NM_001181848.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YLL028W_mRNA; YLL028W_mRNA; YLL028W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
850631

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YLL028W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73133 Genomic DNA Translation: CAA97477.1
BK006945 Genomic DNA Translation: DAA09292.1
PIRiS64779
RefSeqiNP_013072.1, NM_001181848.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

BioGridi31224, 138 interactors
DIPiDIP-1706N
IntActiQ07824, 29 interactors
MINTiQ07824
STRINGi4932.YLL028W

Protein family/group databases

TCDBi2.A.1.2.16 the major facilitator superfamily (mfs)

PTM databases

iPTMnetiQ07824

Proteomic databases

MaxQBiQ07824
PaxDbiQ07824
PRIDEiQ07824

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL028W_mRNA; YLL028W_mRNA; YLL028W
GeneIDi850631
KEGGisce:YLL028W

Organism-specific databases

EuPathDBiFungiDB:YLL028W
SGDiS000003951 TPO1

Phylogenomic databases

HOGENOMiHOG000160689
InParanoidiQ07824
KOiK08157
OMAiLSIYMSF

Enzyme and pathway databases

BioCyciYEAST:G3O-32132-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q07824

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTPO1_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q07824
Secondary accession number(s): D6VXX6
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: June 5, 2019
This is version 150 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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