UniProtKB - Q07666 (KHDR1_HUMAN)
KH domain-containing, RNA-binding, signal transduction-associated protein 1
KHDRBS1
Functioni
GO - Molecular functioni
- DNA binding Source: ProtInc
- identical protein binding Source: UniProtKB
- mRNA binding Source: GO_Central
- poly(A) binding Source: MGI
- poly(U) RNA binding Source: MGI
- protein-containing complex binding Source: Ensembl
- protein domain specific binding Source: UniProtKB
- protein tyrosine kinase binding Source: UniProtKB
- RNA binding Source: UniProtKB
- SH2 domain binding Source: UniProtKB
- SH3 domain binding Source: UniProtKB
- signaling adaptor activity Source: UniProtKB
GO - Biological processi
- G1/S transition of mitotic cell cycle Source: GO_Central
- G2/M transition of mitotic cell cycle Source: UniProtKB
- mRNA processing Source: ProtInc
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: Ensembl
- positive regulation of RNA export from nucleus Source: UniProtKB
- positive regulation of translational initiation Source: UniProtKB
- regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
- regulation of mRNA splicing, via spliceosome Source: GO_Central
- regulation of RNA export from nucleus Source: UniProtKB
- spermatogenesis Source: Ensembl
- T cell receptor signaling pathway Source: UniProtKB
Keywordsi
Molecular function | RNA-binding |
Biological process | Cell cycle, mRNA processing, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q07666 |
Reactomei | R-HSA-8849468, PTK6 Regulates Proteins Involved in RNA Processing |
SignaLinki | Q07666 |
SIGNORi | Q07666 |
Names & Taxonomyi
Protein namesi | Recommended name: KH domain-containing, RNA-binding, signal transduction-associated protein 1Alternative name(s): GAP-associated tyrosine phosphoprotein p62 Src-associated in mitosis 68 kDa protein Short name: Sam68 p21 Ras GTPase-activating protein-associated p62 p68 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18116, KHDRBS1 |
MIMi | 602489, gene |
neXtProti | NX_Q07666 |
VEuPathDBi | HostDB:ENSG00000121774.17 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Plasma Membrane
- Grb2-Sos complex Source: Ensembl
Other locations
- cytoplasm Source: UniProtKB
- membrane Source: UniProtKB
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 103 | Y → S: Impairs homodimerization. 1 Publication | 1 | |
Mutagenesisi | 110 | E → A: Impairs homodimerization. 1 Publication | 1 | |
Mutagenesisi | 118 | F → S: Disrupts homodimerization, impairs influence on alternative splicing. 1 Publication | 1 | |
Mutagenesisi | 229 | V → F: Disrupts binding to poly(A). Decreased binding to the BCL2L1 mRNA. Loss of function in BCL2L1 splicing. Changed nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 241 | Y → E: Fails to influence alternative splicing of CD44, NRXN2 and NRXN3. 1 Publication | 1 | |
Mutagenesisi | 381 | E → K: Disrupts interaction with APC. 1 Publication | 1 | |
Mutagenesisi | 383 | Y → K: Impairs interaction with APC. 1 Publication | 1 | |
Mutagenesisi | 384 | E → K: Disrupts interaction with APC. 1 Publication | 1 | |
Mutagenesisi | 435 | Y → F: No effect on the nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 440 | Y → F: Completely blocks nuclear localization. 1 Publication | 1 | |
Mutagenesisi | 443 | Y → F: No effect on the nuclear localization. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10657 |
OpenTargetsi | ENSG00000121774 |
PharmGKBi | PA30092 |
Miscellaneous databases
Pharosi | Q07666, Tbio |
Genetic variation databases
BioMutai | KHDRBS1 |
DMDMi | 62511098 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050124 | 1 – 443 | KH domain-containing, RNA-binding, signal transduction-associated protein 1Add BLAST | 443 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 18 | PhosphoserineCombined sources | 1 | |
Modified residuei | 20 | PhosphoserineCombined sources | 1 | |
Modified residuei | 29 | PhosphoserineCombined sources | 1 | |
Modified residuei | 33 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 45 | Asymmetric dimethylarginine; by PRMT11 Publication | 1 | |
Modified residuei | 52 | Asymmetric dimethylarginine; partial; by PRMT11 Publication | 1 | |
Modified residuei | 58 | PhosphoserineCombined sources | 1 | |
Modified residuei | 84 | Phosphothreonine; by MAPK1By similarity | 1 | |
Cross-linki | 96 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 102 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 113 | PhosphoserineBy similarity | 1 | |
Cross-linki | 139 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 150 | PhosphoserineCombined sources | 1 | |
Modified residuei | 175 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 175 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources | ||
Modified residuei | 183 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 282 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 284 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 291 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 304 | Asymmetric dimethylarginine; by PRMT11 Publication | 1 | |
Modified residuei | 310 | Omega-N-methylarginine; by PRMT11 Publication | 1 | |
Modified residuei | 315 | Omega-N-methylarginine; by PRMT11 Publication | 1 | |
Modified residuei | 320 | Dimethylated arginine; in A2780 ovarian carcinoma cell line1 Publication | 1 | |
Modified residuei | 320 | Omega-N-methylarginine; by PRMT11 Publication | 1 | |
Modified residuei | 325 | Omega-N-methylarginine; by PRMT11 Publication | 1 | |
Modified residuei | 331 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 331 | Dimethylated arginine; in A2780 ovarian carcinoma cell line1 Publication | 1 | |
Modified residuei | 331 | Omega-N-methylarginine; by PRMT1; alternateCombined sources1 Publication | 1 | |
Modified residuei | 340 | Dimethylated arginine; in A2780 ovarian carcinoma cell line1 Publication | 1 | |
Modified residuei | 340 | Omega-N-methylarginine; by PRMT1Combined sources | 1 | |
Modified residuei | 387 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 390 | PhosphoserineCombined sources | 1 | |
Cross-linki | 432 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 435 | Phosphotyrosine; by PTK61 Publication | 1 | |
Modified residuei | 440 | Phosphotyrosine; by PTK61 Publication | 1 | |
Modified residuei | 443 | Phosphotyrosine; by PTK61 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q07666 |
jPOSTi | Q07666 |
MassIVEi | Q07666 |
MaxQBi | Q07666 |
PaxDbi | Q07666 |
PeptideAtlasi | Q07666 |
PRIDEi | Q07666 |
ProteomicsDBi | 58523 [Q07666-1] 58524 [Q07666-2] 58525 [Q07666-3] |
TopDownProteomicsi | Q07666-1 [Q07666-1] Q07666-3 [Q07666-3] |
PTM databases
iPTMneti | Q07666 |
MetOSitei | Q07666 |
PhosphoSitePlusi | Q07666 |
SwissPalmi | Q07666 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSG00000121774, Expressed in forebrain and 250 other tissues |
Genevisiblei | Q07666, HS |
Organism-specific databases
HPAi | ENSG00000121774, Low tissue specificity |
Interactioni
Subunit structurei
Self-associates to form homooligomers when bound to RNA, oligomerization appears to be limited when binding to proteins; dimerization increases RNA affinity (PubMed:26758068, PubMed:20610388).
Interacts with KHDRBS3/SLIM-2 (PubMed:10332027).
Interacts with KHDRBS2/SLIM-1; heterooligomer formation of KHDRBS family proteins may modulate RNA substrate specificity (By similarity).
Interacts with RASA1, LCK, FYN, PTPN6, PLCG1, GRB2, CBL, JAK3, PIK3R, STAT3, APC, HNRNPA1 (PubMed:1374686, PubMed:9045636, PubMed:10332027, PubMed:11585385, PubMed:17371836, PubMed:22000517).
Interacts with PTK6 (via SH3 and SH2 domains) (PubMed:10913193).
Forms a complex with ILF2, ILF3, YLPM1, RBMX, NCOA5 and PPP1CA (PubMed:17890166). Does not interact with TPR (PubMed:22253824).
Interacts with RBMY1A1, PRMT1 (By similarity). Binds WBP4/FBP21 (via WW domains), FNBP4/FBP30 (via WW domains).
Interacts (via Arg/Gly-rich-flanked Pro-rich regions) with FYN (via the SH3 domain) (By similarity).
Interacts with the non-receptor tyrosine kinase SRMS; the interaction leads to phosphorylation of KHDRBS1 (PubMed:29496907).
Interacts with ZBTB7A; negatively regulates KHDRBS1 splicing activity toward BCL2L1 (PubMed:24514149).
By similarity10 PublicationsBinary interactionsi
Hide detailsQ07666
GO - Molecular functioni
- identical protein binding Source: UniProtKB
- protein domain specific binding Source: UniProtKB
- protein tyrosine kinase binding Source: UniProtKB
- SH2 domain binding Source: UniProtKB
- SH3 domain binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 115900, 253 interactors |
CORUMi | Q07666 |
DIPi | DIP-29007N |
IntActi | Q07666, 132 interactors |
MINTi | Q07666 |
STRINGi | 9606.ENSP00000313829 |
Miscellaneous databases
RNActi | Q07666, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q07666 |
SMRi | Q07666 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q07666 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 171 – 197 | KHPROSITE-ProRule annotationAdd BLAST | 27 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 100 – 260 | Involved in homodimerization1 PublicationAdd BLAST | 161 | |
Regioni | 351 – 443 | Interaction with HNRNPA11 PublicationAdd BLAST | 93 | |
Regioni | 400 – 420 | Interaction with ZBTB7A1 PublicationAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 34 – 41 | Pro-richSequence analysis | 8 | |
Compositional biasi | 44 – 55 | DMA/Gly-richSequence analysisAdd BLAST | 12 | |
Compositional biasi | 59 – 89 | Pro-richSequence analysisAdd BLAST | 31 | |
Compositional biasi | 282 – 292 | DMA/Gly-richSequence analysisAdd BLAST | 11 | |
Compositional biasi | 295 – 301 | Pro-richSequence analysis | 7 | |
Compositional biasi | 302 – 332 | Arg/Gly-richSequence analysisAdd BLAST | 31 | |
Compositional biasi | 334 – 363 | Pro-richSequence analysisAdd BLAST | 30 |
Domaini
Sequence similaritiesi
Keywords - Domaini
SH3-bindingPhylogenomic databases
eggNOGi | KOG1588, Eukaryota |
GeneTreei | ENSGT00940000155718 |
HOGENOMi | CLU_034976_0_0_1 |
InParanoidi | Q07666 |
OMAi | GQDHGAR |
OrthoDBi | 1012406at2759 |
PhylomeDBi | Q07666 |
TreeFami | TF314878 |
Family and domain databases
DisProti | DP01705 |
Gene3Di | 3.30.1370.10, 1 hit |
IDEALi | IID00619 |
InterProi | View protein in InterPro IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR032571, Qua1_dom IPR032335, Sam68-YY |
Pfami | View protein in Pfam PF00013, KH_1, 1 hit PF16274, Qua1, 1 hit PF16568, Sam68-YY, 1 hit |
SMARTi | View protein in SMART SM00322, KH, 1 hit |
SUPFAMi | SSF54791, SSF54791, 1 hit |
PROSITEi | View protein in PROSITE PS50084, KH_TYPE_1, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQRRDDPAAR MSRSSGRSGS MDPSGAHPSV RQTPSRQPPL PHRSRGGGGG
60 70 80 90 100
SRGGARASPA TQPPPLLPPS ATGPDATVGG PAPTPLLPPS ATASVKMEPE
110 120 130 140 150
NKYLPELMAE KDSLDPSFTH AMQLLTAEIE KIQKGDSKKD DEENYLDLFS
160 170 180 190 200
HKNMKLKERV LIPVKQYPKF NFVGKILGPQ GNTIKRLQEE TGAKISVLGK
210 220 230 240 250
GSMRDKAKEE ELRKGGDPKY AHLNMDLHVF IEVFGPPCEA YALMAHAMEE
260 270 280 290 300
VKKFLVPDMM DDICQEQFLE LSYLNGVPEP SRGRGVPVRG RGAAPPPPPV
310 320 330 340 350
PRGRGVGPPR GALVRGTPVR GAITRGATVT RGVPPPPTVR GAPAPRARTA
360 370 380 390 400
GIQRIPLPPP PAPETYEEYG YDDTYAEQSY EGYEGYYSQS QGDSEYYDYG
410 420 430 440
HGEVQDSYEA YGQDDWNGTR PSLKAPPARP VKGAYREHPY GRY
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_051719 | 37 – 61 | Missing in isoform 2. 1 PublicationAdd BLAST | 25 | |
Alternative sequenceiVSP_051720 | 169 – 207 | Missing in isoform 3. 1 PublicationAdd BLAST | 39 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88108 mRNA Translation: AAA59990.1 U78971 mRNA Translation: AAB47504.1 AK091346 mRNA Translation: BAC03643.1 AL139249 Genomic DNA No translation available. AL445248 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX07576.1 CH471059 Genomic DNA Translation: EAX07577.1 BC000717 mRNA Translation: AAH00717.1 BC010132 mRNA Translation: AAH10132.1 Sequence problems. BC019109 mRNA Translation: AAH19109.1 |
CCDSi | CCDS350.1 [Q07666-1] CCDS60067.1 [Q07666-3] |
PIRi | A38219 |
RefSeqi | NP_001258807.1, NM_001271878.1 [Q07666-3] NP_006550.1, NM_006559.2 [Q07666-1] |
Genome annotation databases
Ensembli | ENST00000327300; ENSP00000313829; ENSG00000121774 [Q07666-1] ENST00000492989; ENSP00000417731; ENSG00000121774 [Q07666-3] |
GeneIDi | 10657 |
KEGGi | hsa:10657 |
UCSCi | uc001bua.3, human [Q07666-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88108 mRNA Translation: AAA59990.1 U78971 mRNA Translation: AAB47504.1 AK091346 mRNA Translation: BAC03643.1 AL139249 Genomic DNA No translation available. AL445248 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX07576.1 CH471059 Genomic DNA Translation: EAX07577.1 BC000717 mRNA Translation: AAH00717.1 BC010132 mRNA Translation: AAH10132.1 Sequence problems. BC019109 mRNA Translation: AAH19109.1 |
CCDSi | CCDS350.1 [Q07666-1] CCDS60067.1 [Q07666-3] |
PIRi | A38219 |
RefSeqi | NP_001258807.1, NM_001271878.1 [Q07666-3] NP_006550.1, NM_006559.2 [Q07666-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2XA6 | NMR | - | A/B | 97-135 | [»] | |
3QHE | X-ray | 2.40 | B/D | 365-419 | [»] | |
BMRBi | Q07666 | |||||
SMRi | Q07666 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115900, 253 interactors |
CORUMi | Q07666 |
DIPi | DIP-29007N |
IntActi | Q07666, 132 interactors |
MINTi | Q07666 |
STRINGi | 9606.ENSP00000313829 |
PTM databases
iPTMneti | Q07666 |
MetOSitei | Q07666 |
PhosphoSitePlusi | Q07666 |
SwissPalmi | Q07666 |
Genetic variation databases
BioMutai | KHDRBS1 |
DMDMi | 62511098 |
Proteomic databases
EPDi | Q07666 |
jPOSTi | Q07666 |
MassIVEi | Q07666 |
MaxQBi | Q07666 |
PaxDbi | Q07666 |
PeptideAtlasi | Q07666 |
PRIDEi | Q07666 |
ProteomicsDBi | 58523 [Q07666-1] 58524 [Q07666-2] 58525 [Q07666-3] |
TopDownProteomicsi | Q07666-1 [Q07666-1] Q07666-3 [Q07666-3] |
Protocols and materials databases
Antibodypediai | 3944, 320 antibodies |
DNASUi | 10657 |
Genome annotation databases
Ensembli | ENST00000327300; ENSP00000313829; ENSG00000121774 [Q07666-1] ENST00000492989; ENSP00000417731; ENSG00000121774 [Q07666-3] |
GeneIDi | 10657 |
KEGGi | hsa:10657 |
UCSCi | uc001bua.3, human [Q07666-1] |
Organism-specific databases
CTDi | 10657 |
DisGeNETi | 10657 |
GeneCardsi | KHDRBS1 |
HGNCi | HGNC:18116, KHDRBS1 |
HPAi | ENSG00000121774, Low tissue specificity |
MIMi | 602489, gene |
neXtProti | NX_Q07666 |
OpenTargetsi | ENSG00000121774 |
PharmGKBi | PA30092 |
VEuPathDBi | HostDB:ENSG00000121774.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1588, Eukaryota |
GeneTreei | ENSGT00940000155718 |
HOGENOMi | CLU_034976_0_0_1 |
InParanoidi | Q07666 |
OMAi | GQDHGAR |
OrthoDBi | 1012406at2759 |
PhylomeDBi | Q07666 |
TreeFami | TF314878 |
Enzyme and pathway databases
PathwayCommonsi | Q07666 |
Reactomei | R-HSA-8849468, PTK6 Regulates Proteins Involved in RNA Processing |
SignaLinki | Q07666 |
SIGNORi | Q07666 |
Miscellaneous databases
BioGRID-ORCSi | 10657, 44 hits in 1002 CRISPR screens |
ChiTaRSi | KHDRBS1, human |
EvolutionaryTracei | Q07666 |
GeneWikii | KHDRBS1 |
GenomeRNAii | 10657 |
Pharosi | Q07666, Tbio |
PROi | PR:Q07666 |
RNActi | Q07666, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000121774, Expressed in forebrain and 250 other tissues |
Genevisiblei | Q07666, HS |
Family and domain databases
DisProti | DP01705 |
Gene3Di | 3.30.1370.10, 1 hit |
IDEALi | IID00619 |
InterProi | View protein in InterPro IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR032571, Qua1_dom IPR032335, Sam68-YY |
Pfami | View protein in Pfam PF00013, KH_1, 1 hit PF16274, Qua1, 1 hit PF16568, Sam68-YY, 1 hit |
SMARTi | View protein in SMART SM00322, KH, 1 hit |
SUPFAMi | SSF54791, SSF54791, 1 hit |
PROSITEi | View protein in PROSITE PS50084, KH_TYPE_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KHDR1_HUMAN | |
Accessioni | Q07666Primary (citable) accession number: Q07666 Secondary accession number(s): D3DPP3 Q99760 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 12, 2005 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 208 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families