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Protein

Seventh homolog of septin 1

Gene

SHS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Septins are GTPases involved in cytokinesis that assemble early in the cell cycle as a patch at the incipient bud site and form a ring approximate 15 min before bud emergence, which transforms into an hour-glass shaped collar of cortical filaments that spans both sides of the mother-bud neck. This collar persists until just before cytokinesis, when it splits into two rings that occupy opposite sides of the neck. The septins at the bud neck serve as a structural scaffold that recruits different components involved in diverse processes at specific stages during the cell cycle. Many proteins bind asymmetrically to the septin collar. The septin assembly is regulated by protein kinases GIN4 and/or CLA4. May act by recruiting MYO1 and HOF1, a protein involved in septation, to the site of cleavage. Septins are also involved in cell morphogenesis, bud site selection, chitin deposition, cell cycle regulation, cell compartmentalization and spore wall formation.1 Publication

Miscellaneous

Present with 5620 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei138GTP; via amide nitrogenBy similarity1
Binding sitei288GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 37GTPBy similarity8
Nucleotide bindingi218 – 226GTPBy similarity9

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: SGD
  • protein-containing complex scaffold activity Source: GO_Central

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29605-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Seventh homolog of septin 1
Alternative name(s):
Septation protein 7
Gene namesi
Name:SHS1
Synonyms:SEP7
Ordered Locus Names:YDL225W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

SGDiS000002384 SHS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi426K → R: Abolishes sumoylation in vitro; when associated with R-437. 1 Publication1
Mutagenesisi437K → R: Abolishes sumoylation in vitro; when associated with R-426. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001735452 – 551Seventh homolog of septin 1Add BLAST550

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei400PhosphotyrosineCombined sources1
Modified residuei408PhosphoserineCombined sources1
Modified residuei416PhosphoserineCombined sources1
Cross-linki426Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-linki437Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Modified residuei447PhosphoserineCombined sources1
Modified residuei460PhosphoserineCombined sources1
Modified residuei519PhosphoserineCombined sources1
Modified residuei520PhosphoserineCombined sources1
Modified residuei522PhosphoserineCombined sources1
Modified residuei525PhosphoserineCombined sources1
Modified residuei539PhosphothreonineCombined sources1
Modified residuei545PhosphoserineCombined sources1
Modified residuei548PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by GIN4 and CLA4. Phosphorylation state is essential for septin ring dynamics during telophase.2 Publications
Sumoylated during mitosis on the mother cell side of the bud neck. Sumoylation probably plays a central role in regulating septin ring disassembly during the cell cycle.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ07657
PaxDbiQ07657
PRIDEiQ07657

PTM databases

iPTMnetiQ07657

Interactioni

Subunit structurei

Component of the septin complex which consists of CDC3, CDC10, CDC11, CDC12 and probably SHS1 and rearranges to a cortical collar of highly ordered filaments at the mother-bud-neck. A complex formed by CDC3, CDC10, CDC11 and CDC12 is capable of forming long filaments in vitro and the components seem to be present in a 2:2:2:2 arrangement in vivo. The filaments are proposed to be formed by the end-to-end polymerization of CDC3-CDC12-CDC11 complexes with CDC10 serving as a bridge to bundle the polymers into paired filaments. Component of the GIN4 complex composed of at least BNI5, CDC3, CDC10, CDC11, CDC12, GIN4, NAP1 and SHS1. Self-associates. Interacts with CDC11 and SPA2.4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi31885, 272 interactors
ComplexPortaliCPX-1675 Septin complex
CPX-1712 Gin4 complex
DIPiDIP-5596N
ELMiQ07657
IntActiQ07657, 54 interactors
MINTiQ07657
STRINGi4932.YDL225W

Structurei

3D structure databases

ProteinModelPortaliQ07657
SMRiQ07657
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 339Septin-type GPROSITE-ProRule annotationAdd BLAST320

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni30 – 37G1 motifPROSITE-ProRule annotation8
Regioni135 – 138G3 motifPROSITE-ProRule annotation4
Regioni217 – 220G4 motifPROSITE-ProRule annotation4

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili418 – 518Sequence analysisAdd BLAST101

Sequence similaritiesi

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00880000138554
HOGENOMiHOG000233586
InParanoidiQ07657
KOiK16946
OMAiYVLRHER
OrthoDBiEOG092C3HL6

Family and domain databases

CDDicd01850 CDC_Septin, 1 hit
InterProiView protein in InterPro
IPR030379 G_SEPTIN_dom
IPR027417 P-loop_NTPase
IPR016491 Septin
PfamiView protein in Pfam
PF00735 Septin, 1 hit
PIRSFiPIRSF006698 Septin, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51719 G_SEPTIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q07657-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTASTPPIN LFRRKKEHKR GITYTMLLCG PAGTGKTAFA NNLLETKIFP
60 70 80 90 100
HKYQYGKSNA SISSNPEVKV IAPTKVVSFN SKNGIPSYVS EFDPMRANLE
110 120 130 140 150
PGITITSTSL ELGGNKDQGK PEMNEDDTVF FNLIMTHGIG ENLDDSLCSE
160 170 180 190 200
EVMSYLEQQF DIVLAEETRI KRNPRFEDTR VHVALYFIEP TGHGLREVDV
210 220 230 240 250
ELMKSISKYT NVLPIITRAD SFTKEELTQF RKNIMFDVER YNVPIYKFEV
260 270 280 290 300
DPEDDDLESM EENQALASLQ PFAIITSDTR DSEGRYVREY PWGIISIDDD
310 320 330 340 350
KISDLKVLKN VLFGSHLQEF KDTTQNLLYE NYRSEKLSSV ANAEEIGPNS
360 370 380 390 400
TKRQSNAPSL SNFASLISTG QFNSSQTLAN NLRADTPRNQ VSGNFKENEY
410 420 430 440 450
EDNGEHDSAE NEQEMSPVRQ LGREIKQENE NLIRSIKTES SPKFLNSPDL
460 470 480 490 500
PERTKLRNIS ETVPYVLRHE RILARQQKLE ELEAQSAKEL QKRIQELERK
510 520 530 540 550
AHELKLREKL INQNKLNGSS SSINSLQQST RSQIKKNDTY TDLASIASGR

D
Length:551
Mass (Da):62,630
Last modified:November 1, 1997 - v1
Checksum:iBDA2631061A278F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74273 Genomic DNA Translation: CAA98804.1
BK006938 Genomic DNA Translation: DAA11640.1
PIRiS67788
RefSeqiNP_010056.1, NM_001180285.1

Genome annotation databases

EnsemblFungiiYDL225W; YDL225W; YDL225W
GeneIDi851373
KEGGisce:YDL225W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74273 Genomic DNA Translation: CAA98804.1
BK006938 Genomic DNA Translation: DAA11640.1
PIRiS67788
RefSeqiNP_010056.1, NM_001180285.1

3D structure databases

ProteinModelPortaliQ07657
SMRiQ07657
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31885, 272 interactors
ComplexPortaliCPX-1675 Septin complex
CPX-1712 Gin4 complex
DIPiDIP-5596N
ELMiQ07657
IntActiQ07657, 54 interactors
MINTiQ07657
STRINGi4932.YDL225W

PTM databases

iPTMnetiQ07657

Proteomic databases

MaxQBiQ07657
PaxDbiQ07657
PRIDEiQ07657

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL225W; YDL225W; YDL225W
GeneIDi851373
KEGGisce:YDL225W

Organism-specific databases

SGDiS000002384 SHS1

Phylogenomic databases

GeneTreeiENSGT00880000138554
HOGENOMiHOG000233586
InParanoidiQ07657
KOiK16946
OMAiYVLRHER
OrthoDBiEOG092C3HL6

Enzyme and pathway databases

BioCyciYEAST:G3O-29605-MONOMER

Miscellaneous databases

PROiPR:Q07657

Family and domain databases

CDDicd01850 CDC_Septin, 1 hit
InterProiView protein in InterPro
IPR030379 G_SEPTIN_dom
IPR027417 P-loop_NTPase
IPR016491 Septin
PfamiView protein in Pfam
PF00735 Septin, 1 hit
PIRSFiPIRSF006698 Septin, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51719 G_SEPTIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSHS1_YEAST
AccessioniPrimary (citable) accession number: Q07657
Secondary accession number(s): D6VRD0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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Main funding by: National Institutes of Health

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