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Protein

NADPH-dependent alpha-keto amide reductase

Gene

YDL124W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reduces aromatic alpha-keto amides, aliphatic and aromatic alpha-keto esters, but not beta-keto esters.1 Publication

Miscellaneous

Present with 4030 molecules/cell in log phase SD medium.1 Publication

Kineticsi

  1. KM=146 µM for o-chlorobenzoylformamide1 Publication
  2. KM=156 µM for m-chlorobenzoylformamide1 Publication
  3. KM=231 µM for p-chlorobenzoylformamide1 Publication
  4. KM=103 µM for benzoylformamide1 Publication
  5. KM=254 µM for 3-methyl-2-oxobutanoate1 Publication

    pH dependencei

    Stable from pH 6 to 9.5.1 Publication

    Temperature dependencei

    Thermostable up to 40 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei64Proton donorBy similarity1
    Sitei89Lowers pKa of active site TyrBy similarity1
    Binding sitei122SubstrateBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi208 – 268NADPBy similarityAdd BLAST61

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    LigandNADP

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29523-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH-dependent alpha-keto amide reductase (EC:1.2.1.-)
    Short name:
    AKR-E
    Alternative name(s):
    YKAR
    Gene namesi
    Ordered Locus Names:YDL124W
    ORF Names:D2240
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome IV

    Organism-specific databases

    EuPathDBiFungiDB:YDL124W
    SGDiS000002282 YDL124W

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002627551 – 312NADPH-dependent alpha-keto amide reductaseAdd BLAST312

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei123PhosphoserineCombined sources1

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ07551
    PaxDbiQ07551
    PRIDEiQ07551
    TopDownProteomicsiQ07551

    2D gel databases

    UCD-2DPAGEiQ07551

    PTM databases

    iPTMnetiQ07551

    Expressioni

    Inductioni

    Transiently induced shortly after the switch from aerobic to anaerobic growth (at protein level).1 Publication

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi31939, 77 interactors
    DIPiDIP-6607N
    IntActiQ07551, 2 interactors
    STRINGi4932.YDL124W

    Structurei

    3D structure databases

    ProteinModelPortaliQ07551
    SMRiQ07551
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldo/keto reductase family.Curated

    Phylogenomic databases

    HOGENOMiHOG000250272
    InParanoidiQ07551
    OMAiNEAWAIM
    OrthoDBiEOG092C324N

    Family and domain databases

    CDDicd06660 Aldo_ket_red, 1 hit
    Gene3Di3.20.20.100, 1 hit
    InterProiView protein in InterPro
    IPR018170 Aldo/ket_reductase_CS
    IPR020471 Aldo/keto_reductase
    IPR023210 NADP_OxRdtase_dom
    IPR036812 NADP_OxRdtase_dom_sf
    PANTHERiPTHR11732 PTHR11732, 1 hit
    PfamiView protein in Pfam
    PF00248 Aldo_ket_red, 1 hit
    PIRSFiPIRSF000097 AKR, 1 hit
    PRINTSiPR00069 ALDKETRDTASE
    SUPFAMiSSF51430 SSF51430, 1 hit
    PROSITEiView protein in PROSITE
    PS00062 ALDOKETO_REDUCTASE_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q07551-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MSFHQQFFTL NNGNKIPAIA IIGTGTRWYK NEETDATFSN SLVEQIVYAL
    60 70 80 90 100
    KLPGIIHIDA AEIYRTYPEV GKALSLTEKP RNAIFLTDKY SPQIKMSDSP
    110 120 130 140 150
    ADGLDLALKK MGTDYVDLYL LHSPFVSKEV NGLSLEEAWK DMEQLYKSGK
    160 170 180 190 200
    AKNIGVSNFA VEDLQRILKV AEVKPQVNQI EFSPFLQNQT PGIYKFCQEH
    210 220 230 240 250
    DILVEAYSPL GPLQKKTAQD DSQPFFEYVK ELSEKYIKSE AQIILRWVTK
    260 270 280 290 300
    RGVLPVTTSS KPQRISDAQN LFSFDLTAEE VDKITELGLE HEPLRLYWNK
    310
    LYGKYNYAAQ KV
    Length:312
    Mass (Da):35,561
    Last modified:November 1, 1996 - v1
    Checksum:i165ADCB5C1952AF0
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z74172 Genomic DNA Translation: CAA98692.1
    BK006938 Genomic DNA Translation: DAA11736.1
    PIRiS67667
    RefSeqiNP_010159.1, NM_001180183.1

    Genome annotation databases

    EnsemblFungiiYDL124W; YDL124W; YDL124W
    GeneIDi851433
    KEGGisce:YDL124W

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z74172 Genomic DNA Translation: CAA98692.1
    BK006938 Genomic DNA Translation: DAA11736.1
    PIRiS67667
    RefSeqiNP_010159.1, NM_001180183.1

    3D structure databases

    ProteinModelPortaliQ07551
    SMRiQ07551
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi31939, 77 interactors
    DIPiDIP-6607N
    IntActiQ07551, 2 interactors
    STRINGi4932.YDL124W

    PTM databases

    iPTMnetiQ07551

    2D gel databases

    UCD-2DPAGEiQ07551

    Proteomic databases

    MaxQBiQ07551
    PaxDbiQ07551
    PRIDEiQ07551
    TopDownProteomicsiQ07551

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYDL124W; YDL124W; YDL124W
    GeneIDi851433
    KEGGisce:YDL124W

    Organism-specific databases

    EuPathDBiFungiDB:YDL124W
    SGDiS000002282 YDL124W

    Phylogenomic databases

    HOGENOMiHOG000250272
    InParanoidiQ07551
    OMAiNEAWAIM
    OrthoDBiEOG092C324N

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29523-MONOMER

    Miscellaneous databases

    PROiPR:Q07551

    Family and domain databases

    CDDicd06660 Aldo_ket_red, 1 hit
    Gene3Di3.20.20.100, 1 hit
    InterProiView protein in InterPro
    IPR018170 Aldo/ket_reductase_CS
    IPR020471 Aldo/keto_reductase
    IPR023210 NADP_OxRdtase_dom
    IPR036812 NADP_OxRdtase_dom_sf
    PANTHERiPTHR11732 PTHR11732, 1 hit
    PfamiView protein in Pfam
    PF00248 Aldo_ket_red, 1 hit
    PIRSFiPIRSF000097 AKR, 1 hit
    PRINTSiPR00069 ALDKETRDTASE
    SUPFAMiSSF51430 SSF51430, 1 hit
    PROSITEiView protein in PROSITE
    PS00062 ALDOKETO_REDUCTASE_2, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiKAR_YEAST
    AccessioniPrimary (citable) accession number: Q07551
    Secondary accession number(s): D6VRM6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: November 1, 1996
    Last modified: September 12, 2018
    This is version 131 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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