UniProtKB - Q07540 (FRDA_YEAST)
Protein
Frataxin homolog, mitochondrial
Gene
YFH1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Promotes the biosynthesis of heme as well as the assembly and repair of iron-sulfur clusters by delivering Fe2+ to proteins involved in these pathways. Plays a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe2+ to Fe3+. Can store large amounts of the metal in the form of a ferrihydrite mineral by oligomerization. May be involved in regulation of the mitochondrial electron transport chain.6 Publications
Miscellaneous
Present with 1560 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:1.16.3.11 Publication
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: GO_Central
- ferric iron binding Source: GO_Central
- ferrous iron binding Source: SGD
- ferroxidase activity Source: SGD
- identical protein binding Source: IntAct
- iron chaperone activity Source: SGD
GO - Biological processi
- cellular iron ion homeostasis Source: SGD
- glutathione metabolic process Source: SGD
- heme biosynthetic process Source: UniProtKB-KW
- ion transport Source: UniProtKB-KW
- iron incorporation into metallo-sulfur cluster Source: GO_Central
- iron-sulfur cluster assembly Source: SGD
- mitochondrial electron transport, succinate to ubiquinone Source: UniProtKB
- mitochondrion organization Source: GO_Central
- response to iron(II) ion Source: UniProtKB
- response to oxidative stress Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Heme biosynthesis, Ion transport, Iron storage, Iron transport, Transport |
Ligand | Iron |
Enzyme and pathway databases
BioCyci | MetaCyc:G3O-29519-MONOMER |
Reactomei | R-SCE-1268020, Mitochondrial protein import R-SCE-1362409, Mitochondrial iron-sulfur cluster biogenesis |
Protein family/group databases
TCDBi | 9.B.21.1.2, the frataxin (frataxin) family |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:YFH1 Ordered Locus Names:YDL120W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YDL120W |
SGDi | S000002278, YFH1 |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix 2 Publications
Mitochondrion
- mitochondrial inner membrane Source: Reactome
- mitochondrial intermembrane space Source: Reactome
- mitochondrial matrix Source: SGD
- mitochondrion Source: SGD
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 79 | D → A: Nearly abolishes ferroxidase activity, slows down oligomerization, impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-82. 1 Publication | 1 | |
Mutagenesisi | 82 | D → A: Nearly abolishes ferroxidase activity, slows down oligomerization, impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-79. 1 Publication | 1 | |
Mutagenesisi | 93 | E → A: Impairs oligomerization and iron mineralization. 1 Publication | 1 | |
Mutagenesisi | 93 | E → A: Impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-97 and A-103. 1 Publication | 1 | |
Mutagenesisi | 97 | D → A: Impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-93 and A-103. 1 Publication | 1 | |
Mutagenesisi | 103 | E → A: Impairs resistance to iron-catalyzed oxidative stress, no effect on Fe(2+) delivery and cell growth; when associated with A-93 and A-97. 1 Publication | 1 | |
Mutagenesisi | 122 – 124 | NKQ → ATA: Impairs cell growth, lowers activity of mitochondrial iron-sulfur cluster-containing enzymes, no effect on iron binding and oligomerization. 1 Publication | 3 | |
Mutagenesisi | 129 | Q → A: Impairs cell growth and lowers aconitase activity. 1 Publication | 1 | |
Mutagenesisi | 130 | I → A: Impairs cell growth and lowers aconitase activity. 1 Publication | 1 | |
Mutagenesisi | 131 | W → A: Impairs cell growth, lowers aconitase activity and strongly decreases interaction with ISU1. 1 Publication | 1 | |
Mutagenesisi | 131 | W → F: Lowers aconitase activity and no effexct on interaction with ISU1. 1 Publication | 1 | |
Mutagenesisi | 141 | R → A: Impairs cell growth and lowers aconitase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 21 | MitochondrionCuratedAdd BLAST | 21 | |
ChainiPRO_0000010135 | 22 – 174 | Frataxin homolog intermediate formAdd BLAST | 153 | |
ChainiPRO_0000010136 | 52 – 174 | Frataxin homolog, mitochondrialAdd BLAST | 123 |
Post-translational modificationi
Processed in two steps by mitochondrial processing peptidase (MPP). MPP first cleaves the precursor to intermediate form and subsequently converts the intermediate to mature size protein.
Proteomic databases
MaxQBi | Q07540 |
PaxDbi | Q07540 |
PRIDEi | Q07540 |
Interactioni
Subunit structurei
Monomer; increments in mitochondrial iron uptake induce stepwise assembly of species ranging from trimers to 24-mers.
Interacts with ISU1.
Interacts with YHB1, SDH1, SDH2, AIM45 and CIR1.
7 PublicationsBinary interactionsi
Hide detailsQ07540
With | #Exp. | IntAct |
---|---|---|
ISU1 [Q03020] | 4 | EBI-2206814,EBI-29901 |
itself | 2 | EBI-2206814,EBI-2206814 |
YHB1 [P39676] | 2 | EBI-2206814,EBI-6905 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 31943, 111 interactors |
DIPi | DIP-7485N |
IntActi | Q07540, 11 interactors |
MINTi | Q07540 |
STRINGi | 4932.YDL120W |
Miscellaneous databases
RNActi | Q07540, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q07540 |
SMRi | Q07540 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q07540 |
Family & Domainsi
Sequence similaritiesi
Belongs to the frataxin family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG3413, Eukaryota |
GeneTreei | ENSGT00390000005811 |
HOGENOMi | CLU_080880_2_4_1 |
InParanoidi | Q07540 |
OMAi | MTEHEFM |
Family and domain databases
Gene3Di | 3.30.920.10, 1 hit |
InterProi | View protein in InterPro IPR017789, Frataxin IPR002908, Frataxin/CyaY IPR036524, Frataxin/CyaY_sf IPR020895, Frataxin_CS |
PANTHERi | PTHR16821, PTHR16821, 1 hit |
Pfami | View protein in Pfam PF01491, Frataxin_Cyay, 1 hit |
SMARTi | View protein in SMART SM01219, Frataxin_Cyay, 1 hit |
SUPFAMi | SSF55387, SSF55387, 1 hit |
TIGRFAMsi | TIGR03421, FeS_CyaY, 1 hit TIGR03422, mito_frataxin, 1 hit |
PROSITEi | View protein in PROSITE PS01344, FRATAXIN_1, 1 hit PS50810, FRATAXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q07540-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIKRSLASLV RVSSVMGRRY MIAAAGGERA RFCPAVTNKK NHTVNTFQKR
60 70 80 90 100
FVESSTDGQV VPQEVLNLPL EKYHEEADDY LDHLLDSLEE LSEAHPDCIP
110 120 130 140 150
DVELSHGVMT LEIPAFGTYV INKQPPNKQI WLASPLSGPN RFDLLNGEWV
160 170
SLRNGTKLTD ILTEEVEKAI SKSQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z74168 Genomic DNA Translation: CAA98688.1 AY558160 Genomic DNA Translation: AAS56486.1 BK006938 Genomic DNA Translation: DAA11740.1 |
PIRi | S67663 |
RefSeqi | NP_010163.1, NM_001180179.1 |
Genome annotation databases
EnsemblFungii | YDL120W_mRNA; YDL120W; YDL120W |
GeneIDi | 851437 |
KEGGi | sce:YDL120W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z74168 Genomic DNA Translation: CAA98688.1 AY558160 Genomic DNA Translation: AAS56486.1 BK006938 Genomic DNA Translation: DAA11740.1 |
PIRi | S67663 |
RefSeqi | NP_010163.1, NM_001180179.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2FQL | X-ray | 3.01 | A | 52-174 | [»] | |
2GA5 | NMR | - | A | 53-174 | [»] | |
3OEQ | X-ray | 2.96 | A | 52-174 | [»] | |
3OER | X-ray | 3.20 | A | 52-174 | [»] | |
4EC2 | X-ray | 3.00 | A | 52-174 | [»] | |
5T0V | electron microscopy | 17.50 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X | 52-172 | [»] | |
5TRE | electron microscopy | 15.60 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X | 52-172 | [»] | |
BMRBi | Q07540 | |||||
SMRi | Q07540 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31943, 111 interactors |
DIPi | DIP-7485N |
IntActi | Q07540, 11 interactors |
MINTi | Q07540 |
STRINGi | 4932.YDL120W |
Protein family/group databases
TCDBi | 9.B.21.1.2, the frataxin (frataxin) family |
Proteomic databases
MaxQBi | Q07540 |
PaxDbi | Q07540 |
PRIDEi | Q07540 |
Genome annotation databases
EnsemblFungii | YDL120W_mRNA; YDL120W; YDL120W |
GeneIDi | 851437 |
KEGGi | sce:YDL120W |
Organism-specific databases
EuPathDBi | FungiDB:YDL120W |
SGDi | S000002278, YFH1 |
Phylogenomic databases
eggNOGi | KOG3413, Eukaryota |
GeneTreei | ENSGT00390000005811 |
HOGENOMi | CLU_080880_2_4_1 |
InParanoidi | Q07540 |
OMAi | MTEHEFM |
Enzyme and pathway databases
BioCyci | MetaCyc:G3O-29519-MONOMER |
Reactomei | R-SCE-1268020, Mitochondrial protein import R-SCE-1362409, Mitochondrial iron-sulfur cluster biogenesis |
Miscellaneous databases
EvolutionaryTracei | Q07540 |
PROi | PR:Q07540 |
RNActi | Q07540, protein |
Family and domain databases
Gene3Di | 3.30.920.10, 1 hit |
InterProi | View protein in InterPro IPR017789, Frataxin IPR002908, Frataxin/CyaY IPR036524, Frataxin/CyaY_sf IPR020895, Frataxin_CS |
PANTHERi | PTHR16821, PTHR16821, 1 hit |
Pfami | View protein in Pfam PF01491, Frataxin_Cyay, 1 hit |
SMARTi | View protein in SMART SM01219, Frataxin_Cyay, 1 hit |
SUPFAMi | SSF55387, SSF55387, 1 hit |
TIGRFAMsi | TIGR03421, FeS_CyaY, 1 hit TIGR03422, mito_frataxin, 1 hit |
PROSITEi | View protein in PROSITE PS01344, FRATAXIN_1, 1 hit PS50810, FRATAXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FRDA_YEAST | |
Accessioni | Q07540Primary (citable) accession number: Q07540 Secondary accession number(s): D6VRN0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 184 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names