UniProtKB - Q07532 (IWR1_YEAST)
Protein
RNA polymerase II nuclear localization protein IWR1
Gene
IWR1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Directs RNA polymerase II nuclear import. Binds RNA polymerase II in the active center cleft between the two largest subunits in the cytoplasm. Then uses an N-terminal bipartite nuclear localization signal that may be recognized by karyopherin alpha to direct the polymerase II complex nuclear import. In the nucleus, is displaced from polymerase II complex by transcription initiation factors and nucleic acids, enabling its export and recycling.4 Publications
Miscellaneous
Present with 1080 molecules/cell in log phase SD medium.1 Publication
Caution
Due to the effects of deletion on gene expression, was originally thought to be a general transcription factor (PubMed:19679657). Further studies clearly suggest that it is involved in nuclear localization of the RNA polymerase II complex (PubMed:21504834).2 Publications
GO - Biological processi
- nucleocytoplasmic transport Source: SGD
- protein import into nucleus Source: SGD
Keywordsi
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: RNA polymerase II nuclear localization protein IWR1Alternative name(s): Interacting with RNA polymerase II protein 1 |
Gene namesi | Name:IWR1 Ordered Locus Names:YDL115C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002273, IWR1 |
VEuPathDBi | FungiDB:YDL115C |
Pathology & Biotechi
Disruption phenotypei
Leads to a K1 killer toxin hypersensitivity and pleiotropic effects on gene expression.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000255269 | 2 – 353 | RNA polymerase II nuclear localization protein IWR1Add BLAST | 352 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | Q07532 |
PaxDbi | Q07532 |
PRIDEi | Q07532 |
PTM databases
iPTMneti | Q07532 |
Interactioni
Subunit structurei
Associates with RNA polymerase II.
Part of a complex consisting of RPB1, RBP2, RPB3, RPB4, RPB5, RPB7, SPT5, DST1 and IWR1.
1 PublicationProtein-protein interaction databases
BioGRIDi | 31947, 278 interactors |
DIPi | DIP-6726N |
IntActi | Q07532, 16 interactors |
MINTi | Q07532 |
STRINGi | 4932.YDL115C |
Miscellaneous databases
RNActi | Q07532, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 10 – 43 | Bipartite nuclear localization signalAdd BLAST | 34 |
Sequence similaritiesi
Belongs to the IWR1/SLC7A6OS family.Curated
Phylogenomic databases
eggNOGi | KOG4852, Eukaryota |
HOGENOMi | CLU_044104_0_0_1 |
InParanoidi | Q07532 |
OMAi | EYPRNEF |
Family and domain databases
InterProi | View protein in InterPro IPR040150, Iwr1 IPR013883, TF_Iwr1_dom |
PANTHERi | PTHR28063, PTHR28063, 2 hits |
Pfami | View protein in Pfam PF08574, Iwr1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q07532-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTISTTTAP EFIRVKRRRD EDSVQALLID EGKRVKKQKF IFKLSKTVSS
60 70 80 90 100
ESYQSEQESS TPLLKLAHED HRHFVLEQRK KSRRDSDDEK SQQRLAAEGS
110 120 130 140 150
TVDDDGLPPE INQMVNDYLK LNKGVEKTER KKPSRKYFTG DSAKIASLPS
160 170 180 190 200
LDYVFDIYHL EKIHDDEVAR YNNEKNIGFV KIIEHIDLAL DEESDPNEAR
210 220 230 240 250
SDDEDSNDEN YYQNDYPEDE DDDRSILFGS EGEDIAALGE EIVIGVNKSR
260 270 280 290 300
FSSWNDDKIQ GSNGYHDVEE EYGDLFNRLG GKSDVLKSIN SSNFIDLDGQ
310 320 330 340 350
EGEIEISDNE DDSDEGDDIE YPRNEFFPTD VDDPLAHHRD RIFHQLQKKI
NRS
Sequence cautioni
The sequence ABM97491 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAA98683 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z74163 Genomic DNA Translation: CAA98683.1 Sequence problems. AY558163 Genomic DNA Translation: AAS56489.1 EF123147 mRNA Translation: ABM97491.1 Different initiation. BK006938 Genomic DNA Translation: DAA11745.1 |
PIRi | S67658 |
RefSeqi | NP_010168.4, NM_001180174.1 |
Genome annotation databases
EnsemblFungii | YDL115C_mRNA; YDL115C; YDL115C |
GeneIDi | 851442 |
KEGGi | sce:YDL115C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z74163 Genomic DNA Translation: CAA98683.1 Sequence problems. AY558163 Genomic DNA Translation: AAS56489.1 EF123147 mRNA Translation: ABM97491.1 Different initiation. BK006938 Genomic DNA Translation: DAA11745.1 |
PIRi | S67658 |
RefSeqi | NP_010168.4, NM_001180174.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 31947, 278 interactors |
DIPi | DIP-6726N |
IntActi | Q07532, 16 interactors |
MINTi | Q07532 |
STRINGi | 4932.YDL115C |
PTM databases
iPTMneti | Q07532 |
Proteomic databases
MaxQBi | Q07532 |
PaxDbi | Q07532 |
PRIDEi | Q07532 |
Genome annotation databases
EnsemblFungii | YDL115C_mRNA; YDL115C; YDL115C |
GeneIDi | 851442 |
KEGGi | sce:YDL115C |
Organism-specific databases
SGDi | S000002273, IWR1 |
VEuPathDBi | FungiDB:YDL115C |
Phylogenomic databases
eggNOGi | KOG4852, Eukaryota |
HOGENOMi | CLU_044104_0_0_1 |
InParanoidi | Q07532 |
OMAi | EYPRNEF |
Miscellaneous databases
PROi | PR:Q07532 |
RNActi | Q07532, protein |
Family and domain databases
InterProi | View protein in InterPro IPR040150, Iwr1 IPR013883, TF_Iwr1_dom |
PANTHERi | PTHR28063, PTHR28063, 2 hits |
Pfami | View protein in Pfam PF08574, Iwr1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IWR1_YEAST | |
Accessioni | Q07532Primary (citable) accession number: Q07532 Secondary accession number(s): A2TBP4, D6VRN5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | June 26, 2007 | |
Last modified: | February 10, 2021 | |
This is version 137 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - SIMILARITY comments
Index of protein domains and families - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names