UniProtKB - Q07417 (ACADS_MOUSE)
Protein
Short-chain specific acyl-CoA dehydrogenase, mitochondrial
Gene
Acads
Organism
Mus musculus (Mouse)
Status
Functioni
Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains.By similarity
Catalytic activityi
- a short-chain 2,3-saturated fatty acyl-CoA + H+ + oxidized [electron-transfer flavoprotein] = a short-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]By similarityEC:1.3.8.1By similarityThis reaction proceeds in the forwardBy similarity direction.
- butanoyl-CoA + H+ + oxidized [electron-transfer flavoprotein] = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]By similarityEC:1.3.8.1By similarityThis reaction proceeds in the forwardBy similarity direction.
- H+ + oxidized [electron-transfer flavoprotein] + pentanoyl-CoA = (2E)-pentenoyl-CoA + reduced [electron-transfer flavoprotein]By similarityThis reaction proceeds in the forwardBy similarity direction.
- H+ + hexanoyl-CoA + oxidized [electron-transfer flavoprotein] = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
FADBy similarityNote: Binds 1 FAD per subunit.By similarity
: mitochondrial fatty acid beta-oxidation Pathwayi
This protein is involved in the pathway mitochondrial fatty acid beta-oxidation, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway mitochondrial fatty acid beta-oxidation and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 161 | Substrate; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 297 | FADBy similarity | 1 | |
Binding sitei | 297 | FAD; shared with dimeric partnerBy similarity | 1 | |
Binding sitei | 308 | FADBy similarity | 1 | |
Active sitei | 392 | Proton acceptorBy similarity | 1 | |
Binding sitei | 393 | Substrate; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 152 – 161 | FADBy similarity | 10 | |
Nucleotide bindingi | 185 – 187 | FADBy similarity | 3 | |
Nucleotide bindingi | 365 – 369 | FADBy similarity | 5 | |
Nucleotide bindingi | 394 – 396 | FADBy similarity | 3 |
GO - Molecular functioni
- acyl-CoA dehydrogenase activity Source: UniProtKB
- butyryl-CoA dehydrogenase activity Source: UniProtKB
- fatty-acyl-CoA binding Source: MGI
- flavin adenine dinucleotide binding Source: MGI
- identical protein binding Source: MGI
GO - Biological processi
- butyrate catabolic process Source: MGI
- fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: MGI
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
Reactomei | R-MMU-77350, Beta oxidation of hexanoyl-CoA to butanoyl-CoA R-MMU-77352, Beta oxidation of butanoyl-CoA to acetyl-CoA |
UniPathwayi | UPA00660 |
Names & Taxonomyi
Protein namesi | Recommended name: Short-chain specific acyl-CoA dehydrogenase, mitochondrial (EC:1.3.8.1By similarity)Short name: SCAD Alternative name(s): Butyryl-CoA dehydrogenase |
Gene namesi | Name:Acads |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:87868, Acads |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix By similarity
Cytoskeleton
- centrosome Source: MGI
Mitochondrion
- mitochondrial matrix Source: UniProtKB
- mitochondrial membrane Source: MGI
- mitochondrion Source: MGI
Nucleus
- nucleoplasm Source: MGI
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 24 | MitochondrionBy similarityAdd BLAST | 24 | |
ChainiPRO_0000000499 | 25 – 412 | Short-chain specific acyl-CoA dehydrogenase, mitochondrialAdd BLAST | 388 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 27 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 51 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 51 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 72 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 129 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 129 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 208 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 262 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 262 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 292 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 306 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 306 | N6-succinyllysine; alternateCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q07417 |
jPOSTi | Q07417 |
MaxQBi | Q07417 |
PaxDbi | Q07417 |
PeptideAtlasi | Q07417 |
PRIDEi | Q07417 |
2D gel databases
REPRODUCTION-2DPAGEi | Q07417 |
PTM databases
iPTMneti | Q07417 |
PhosphoSitePlusi | Q07417 |
SwissPalmi | Q07417 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029545, Expressed in brown adipose tissue and 241 other tissues |
Genevisiblei | Q07417, MM |
Interactioni
Subunit structurei
Homotetramer.
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 197915, 15 interactors |
IntActi | Q07417, 6 interactors |
MINTi | Q07417 |
STRINGi | 10090.ENSMUSP00000031524 |
Miscellaneous databases
RNActi | Q07417, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 269 – 272 | Substrate bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the acyl-CoA dehydrogenase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0139, Eukaryota |
GeneTreei | ENSGT00940000158866 |
HOGENOMi | CLU_018204_0_2_1 |
InParanoidi | Q07417 |
OMAi | LVPEQWG |
OrthoDBi | 589058at2759 |
TreeFami | TF105019 |
Family and domain databases
Gene3Di | 1.10.540.10, 1 hit |
InterProi | View protein in InterPro IPR006089, Acyl-CoA_DH_CS IPR006091, Acyl-CoA_Oxase/DH_cen-dom IPR036250, AcylCo_DH-like_C IPR009075, AcylCo_DH/oxidase_C IPR013786, AcylCoA_DH/ox_N IPR037069, AcylCoA_DH/ox_N_sf IPR009100, AcylCoA_DH/oxidase_NM_dom |
Pfami | View protein in Pfam PF00441, Acyl-CoA_dh_1, 1 hit PF02770, Acyl-CoA_dh_M, 1 hit PF02771, Acyl-CoA_dh_N, 1 hit |
SUPFAMi | SSF47203, SSF47203, 1 hit SSF56645, SSF56645, 1 hit |
PROSITEi | View protein in PROSITE PS00072, ACYL_COA_DH_1, 1 hit PS00073, ACYL_COA_DH_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q07417-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAALLARAR GPLRRALGVR DWRRLHTVYQ SVELPETHQM LRQTCRDFAE
60 70 80 90 100
KELVPIAAQL DREHLFPTAQ VKKMGELGLL AMDVPEELSG AGLDYLAYSI
110 120 130 140 150
ALEEISRACA STGVIMSVNN SLYLGPILKF GSAQQKQQWI TPFTNGDKIG
160 170 180 190 200
CFALSEPGNG SDAGAASTTA REEGDSWVLN GTKAWITNSW EASATVVFAS
210 220 230 240 250
TDRSRQNKGI SAFLVPMPTP GLTLGKKEDK LGIRASSTAN LIFEDCRIPK
260 270 280 290 300
ENLLGEPGMG FKIAMQTLDM GRIGIASQAL GIAQASLDCA VKYAENRNAF
310 320 330 340 350
GAPLTKLQNI QFKLADMALA LESARLLTWR AAMLKDNKKP FTKESAMAKL
360 370 380 390 400
AASEAATAIS HQAIQILGGM GYVTEMPAER YYRDARITEI YEGTSEIQRL
410
VIAGHLLRSY RS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 94 | D → G in AAA16714 (PubMed:8276399).Curated | 1 | |
Sequence conflicti | 348 | A → R in AAA16714 (PubMed:8276399).Curated | 1 | |
Sequence conflicti | 369 | G → S in AAA16714 (PubMed:8276399).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L11163 mRNA Translation: AAA16714.1 AK155361 mRNA Translation: BAE33217.1 AK169428 mRNA Translation: BAE41170.1 CH466529 Genomic DNA Translation: EDL19883.1 BC016259 mRNA Translation: AAH16259.1 |
CCDSi | CCDS19579.1 |
PIRi | I49605 |
RefSeqi | NP_031409.2, NM_007383.3 |
Genome annotation databases
Ensembli | ENSMUST00000031524; ENSMUSP00000031524; ENSMUSG00000029545 |
GeneIDi | 11409 |
KEGGi | mmu:11409 |
UCSCi | uc008zdb.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L11163 mRNA Translation: AAA16714.1 AK155361 mRNA Translation: BAE33217.1 AK169428 mRNA Translation: BAE41170.1 CH466529 Genomic DNA Translation: EDL19883.1 BC016259 mRNA Translation: AAH16259.1 |
CCDSi | CCDS19579.1 |
PIRi | I49605 |
RefSeqi | NP_031409.2, NM_007383.3 |
3D structure databases
SMRi | Q07417 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 197915, 15 interactors |
IntActi | Q07417, 6 interactors |
MINTi | Q07417 |
STRINGi | 10090.ENSMUSP00000031524 |
PTM databases
iPTMneti | Q07417 |
PhosphoSitePlusi | Q07417 |
SwissPalmi | Q07417 |
2D gel databases
REPRODUCTION-2DPAGEi | Q07417 |
Proteomic databases
EPDi | Q07417 |
jPOSTi | Q07417 |
MaxQBi | Q07417 |
PaxDbi | Q07417 |
PeptideAtlasi | Q07417 |
PRIDEi | Q07417 |
Protocols and materials databases
Antibodypediai | 1579, 261 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000031524; ENSMUSP00000031524; ENSMUSG00000029545 |
GeneIDi | 11409 |
KEGGi | mmu:11409 |
UCSCi | uc008zdb.2, mouse |
Organism-specific databases
CTDi | 35 |
MGIi | MGI:87868, Acads |
Phylogenomic databases
eggNOGi | KOG0139, Eukaryota |
GeneTreei | ENSGT00940000158866 |
HOGENOMi | CLU_018204_0_2_1 |
InParanoidi | Q07417 |
OMAi | LVPEQWG |
OrthoDBi | 589058at2759 |
TreeFami | TF105019 |
Enzyme and pathway databases
UniPathwayi | UPA00660 |
Reactomei | R-MMU-77350, Beta oxidation of hexanoyl-CoA to butanoyl-CoA R-MMU-77352, Beta oxidation of butanoyl-CoA to acetyl-CoA |
Miscellaneous databases
BioGRID-ORCSi | 11409, 1 hit in 18 CRISPR screens |
PROi | PR:Q07417 |
RNActi | Q07417, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029545, Expressed in brown adipose tissue and 241 other tissues |
Genevisiblei | Q07417, MM |
Family and domain databases
Gene3Di | 1.10.540.10, 1 hit |
InterProi | View protein in InterPro IPR006089, Acyl-CoA_DH_CS IPR006091, Acyl-CoA_Oxase/DH_cen-dom IPR036250, AcylCo_DH-like_C IPR009075, AcylCo_DH/oxidase_C IPR013786, AcylCoA_DH/ox_N IPR037069, AcylCoA_DH/ox_N_sf IPR009100, AcylCoA_DH/oxidase_NM_dom |
Pfami | View protein in Pfam PF00441, Acyl-CoA_dh_1, 1 hit PF02770, Acyl-CoA_dh_M, 1 hit PF02771, Acyl-CoA_dh_N, 1 hit |
SUPFAMi | SSF47203, SSF47203, 1 hit SSF56645, SSF56645, 1 hit |
PROSITEi | View protein in PROSITE PS00072, ACYL_COA_DH_1, 1 hit PS00073, ACYL_COA_DH_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACADS_MOUSE | |
Accessioni | Q07417Primary (citable) accession number: Q07417 Secondary accession number(s): Q91W85 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families