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Protein

Transcription factor NF-E2 45 kDa subunit

Gene

Nfe2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor NF-E2 45 kDa subunit
Alternative name(s):
Leucine zipper protein NF-E2
Nuclear factor, erythroid-derived 2 45 kDa subunit
p45 NF-E2
Gene namesi
Name:Nfe2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97308 Nfe2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi63 – 65PTY → AAA: Loss of transactivation activity. No induction of histone H3 'K-4' acetylation. 1 Publication3
Mutagenesisi157S → A: Loss of MAPK8-mediated phosphorylation and no protein degradation. 1 Publication1
Mutagenesisi170S → A: Abolishes phosphorylation by PKA. No effect on ability to bind DNA nor on transactivation activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003200781 – 373Transcription factor NF-E2 45 kDa subunitAdd BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157Phosphoserine; by MAPK81 Publication1
Modified residuei170Phosphoserine; by PKA1 Publication1
Cross-linki368Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki368Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity

Post-translational modificationi

Phosphorylated on serine residues. In undifferentiated erythrocytes, phosphorylated by MAPK8 which then leads to ubiquitination and protein degradation (By similarity).By similarity
Sumoylated. Sumoylation is required for translocation to nuclear bodies PODs, anchoring to the gene loci, and transactivation of the beta-globin gene (By similarity).By similarity
Ubiquitinated mainly by 'Lys63'-linked ubiquitin (By similarity). Polyubiquitination with 'Lys63'-linked ubiquitin by ITCH retains NFE2 in the cytoplasm preventing its transactivation activity (By similarity). In undifferentiated erythrocyte, ubiquitinated after MAPK8-mediatd phosphorylation leading to protein degradation.By similarity2 Publications

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ07279
PRIDEiQ07279

PTM databases

iPTMnetiQ07279
PhosphoSitePlusiQ07279

Expressioni

Gene expression databases

CleanExiMM_NFE2

Interactioni

Subunit structurei

Homodimer; can bind DNA as a homodimer (By similarity). Erythroid transcription activator nuclear factor erythroid-derived 2 (NF-E2), composed of a heterodimer of NFE2 and MAFK, possesses transactivation activity on beta-globin. Also forms high affinity heterodimer with MAFG; the interaction promotes erythropoiesis. Interacts (via the PXY motif 1) with ITCH (via the WW 1 domain); the interaction promotes 'Lys63'-linked ubiquitination of NFE2, translocates it to the cytoplasm and inhibits its transactivation activity. Interacts with KMT2D/MLL2; the interaction promotes transactivation of the beta-globin locus. Interacts with MAPK8 (phosphorylated form); the interaction leads to phosphorylation of NFE2 in undifferentiated cells.By similarity2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201742, 69 interactors
DIPiDIP-44849N
IntActiQ07279, 4 interactors
STRINGi10090.ENSMUSP00000074684

Structurei

3D structure databases

ProteinModelPortaliQ07279
SMRiQ07279
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini266 – 329bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 206Transactivation domainBy similarityAdd BLAST206
Regioni1 – 83Required for interaction with MAPK8By similarityAdd BLAST83
Regioni268 – 287Basic motifPROSITE-ProRule annotationAdd BLAST20
Regioni291 – 298Leucine-zipperPROSITE-ProRule annotation8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi61 – 65PXY motif 15
Motifi79 – 83PXY motif 25

Domaini

The PXY motifs are required for binding WW domains. PXY1 is required to promote transactivation of beta-globin and for hyperacetylation of histone H3, but not for binding to the HS2 promoter site.

Sequence similaritiesi

Belongs to the bZIP family. CNC subfamily.Curated

Phylogenomic databases

eggNOGiENOG410ISV1 Eukaryota
ENOG410YEZR LUCA
HOGENOMiHOG000234410
HOVERGENiHBG002901
InParanoidiQ07279
KOiK09039
PhylomeDBiQ07279
TreeFamiTF326681

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR004826 bZIP_Maf
IPR029853 NF-E2_p45
IPR008917 TF_DNA-bd_sf
PANTHERiPTHR24411:SF26 PTHR24411:SF26, 2 hits
PfamiView protein in Pfam
PF03131 bZIP_Maf, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF47454 SSF47454, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All

Q07279-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPPCPPQQNR NRLSQLPVGE LGEMELTWQE IMSITELQGL NVPSETSFEP
60 70 80 90 100
QAPTPYPGPL PPPTYCPCSI HPDAGFSLPP PSYELPASTP HVPELPYSYG
110 120 130 140 150
NVAIPVSKPL TLSGLLNEPL PDHLALLDIG LPVGQPKPQE DPESDSGLSL
160 170 180 190 200
NYSDAESLEL EGMEAGRRRS EYVDMYPVEY PYSLMPNSLA HPNYTLPPTE
210 220 230 240 250
TPLALESSSG PVRAKPAVRG EAGSRDERRA LAMKIPFPTD KIVNLPVDDF
260 270 280 290 300
NELLAQYPLT ESQLALVRDI RRRGKNKVAA QNCRKRKLET IVQLERELER
310 320 330 340 350
LSSERERLLR ARGEADRTLE VMRQQLAELY HDIFQHLRDE SGNSYSPEEY
360 370
VLQQAADGAI FLVPRGTKME ATD
Length:373
Mass (Da):41,565
Last modified:November 1, 1996 - v1
Checksum:i47E505F78B6EEB65
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J0Y5A0A0R4J0Y5_MOUSE
Nuclear factor, erythroid derived 2...
Nfe2 mCG_15671
373Annotation score:
D3Z224D3Z224_MOUSE
Transcription factor NF-E2 45 kDa s...
Nfe2
396Annotation score:
D3Z6L9D3Z6L9_MOUSE
Transcription factor NF-E2 45 kDa s...
Nfe2
185Annotation score:
D3YUM3D3YUM3_MOUSE
Transcription factor NF-E2 45 kDa s...
Nfe2
202Annotation score:
D3Z706D3Z706_MOUSE
Transcription factor NF-E2 45 kDa s...
Nfe2
71Annotation score:
D3Z0J1D3Z0J1_MOUSE
Transcription factor NF-E2 45 kDa s...
Nfe2
164Annotation score:
D3Z4E8D3Z4E8_MOUSE
Transcription factor NF-E2 45 kDa s...
Nfe2
108Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173V → A in AAH62171 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09600 mRNA Translation: AAA40417.1
AK156702 mRNA Translation: BAE33812.1
BC062171 mRNA Translation: AAH62171.1
CCDSiCCDS27900.1
PIRiS32537
RefSeqiNP_001289267.1, NM_001302338.1
NP_001289268.1, NM_001302339.1
NP_001289269.1, NM_001302340.1
NP_001289270.1, NM_001302341.1
NP_001289272.1, NM_001302343.1
NP_032711.2, NM_008685.3
XP_006520636.1, XM_006520573.2
UniGeneiMm.457989
Mm.489030

Genome annotation databases

GeneIDi18022
KEGGimmu:18022

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09600 mRNA Translation: AAA40417.1
AK156702 mRNA Translation: BAE33812.1
BC062171 mRNA Translation: AAH62171.1
CCDSiCCDS27900.1
PIRiS32537
RefSeqiNP_001289267.1, NM_001302338.1
NP_001289268.1, NM_001302339.1
NP_001289269.1, NM_001302340.1
NP_001289270.1, NM_001302341.1
NP_001289272.1, NM_001302343.1
NP_032711.2, NM_008685.3
XP_006520636.1, XM_006520573.2
UniGeneiMm.457989
Mm.489030

3D structure databases

ProteinModelPortaliQ07279
SMRiQ07279
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201742, 69 interactors
DIPiDIP-44849N
IntActiQ07279, 4 interactors
STRINGi10090.ENSMUSP00000074684

PTM databases

iPTMnetiQ07279
PhosphoSitePlusiQ07279

Proteomic databases

PaxDbiQ07279
PRIDEiQ07279

Protocols and materials databases

DNASUi18022
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18022
KEGGimmu:18022

Organism-specific databases

CTDi4778
MGIiMGI:97308 Nfe2

Phylogenomic databases

eggNOGiENOG410ISV1 Eukaryota
ENOG410YEZR LUCA
HOGENOMiHOG000234410
HOVERGENiHBG002901
InParanoidiQ07279
KOiK09039
PhylomeDBiQ07279
TreeFamiTF326681

Miscellaneous databases

PROiPR:Q07279
SOURCEiSearch...

Gene expression databases

CleanExiMM_NFE2

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR004826 bZIP_Maf
IPR029853 NF-E2_p45
IPR008917 TF_DNA-bd_sf
PANTHERiPTHR24411:SF26 PTHR24411:SF26, 2 hits
PfamiView protein in Pfam
PF03131 bZIP_Maf, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF47454 SSF47454, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNFE2_MOUSE
AccessioniPrimary (citable) accession number: Q07279
Secondary accession number(s): Q6P6K5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 1, 1996
Last modified: September 12, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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