UniProtKB - Q07181 (PGLR_GIBFU)
Protein
Polygalacturonase
Gene
PGA
Organism
Gibberella fujikuroi (Bakanae and foot rot disease fungus) (Fusarium fujikuroi)
Status
Functioni
Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.
Catalytic activityi
- (1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m). EC:3.2.1.15
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 212 | Proton donorPROSITE-ProRule annotation | 1 | |
Active sitei | 234 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- polygalacturonase activity Source: UniProtKB-EC
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- cell wall organization Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Cell wall biogenesis/degradation |
Enzyme and pathway databases
SABIO-RKi | Q07181 |
Protein family/group databases
CAZyi | GH28, Glycoside Hydrolase Family 28 |
CLAEi | PGA28A_GIBMO |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PGA |
Organismi | Gibberella fujikuroi (Bakanae and foot rot disease fungus) (Fusarium fujikuroi) |
Taxonomic identifieri | 5127 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Fusarium › Fusarium fujikuroi species complex |
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 188 | H → P: Reduces activity by 98.2%. Lowers affinity for substrate 50-fold. 1 Publication | 1 | |
Mutagenesisi | 191 | D → A: Loss of activity. No effect on Km for substrate. 1 Publication | 1 | |
Mutagenesisi | 212 – 213 | DD → EE: Loss of activity. No effect on Km for substrate. 1 Publication | 2 | |
Mutagenesisi | 212 – 213 | DD → NN: Loss of activity. No effect on Km for substrate. 1 Publication | 2 | |
Mutagenesisi | 267 | R → A: Loss of activity. Lowers affinity for substrate over 10-fold. 1 Publication | 1 | |
Mutagenesisi | 269 | K → E: Reduces activity by 99.87%. Lowers affinity for substrate over 10-fold. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
ChainiPRO_0000024786 | 25 – 373 | PolygalacturonaseAdd BLAST | 349 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 27 ↔ 42 | |||
Glycosylationi | 65 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 94 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 214 ↔ 230 | |||
Glycosylationi | 280 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 290 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 340 ↔ 345 | |||
Disulfide bondi | 364 ↔ 371 |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q07181 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q07181 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 136 – 158 | PbH1 1Add BLAST | 23 | |
Repeati | 159 – 197 | PbH1 2Add BLAST | 39 | |
Repeati | 198 – 219 | PbH1 3Add BLAST | 22 | |
Repeati | 220 – 240 | PbH1 4Add BLAST | 21 | |
Repeati | 249 – 270 | PbH1 5Add BLAST | 22 | |
Repeati | 278 – 300 | PbH1 6Add BLAST | 23 | |
Repeati | 312 – 333 | PbH1 7Add BLAST | 22 | |
Repeati | 345 – 369 | PbH1 8Add BLAST | 25 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 28 family.Curated
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502QW1P, Eukaryota |
Family and domain databases
Gene3Di | 2.160.20.10, 1 hit |
InterProi | View protein in InterPro IPR000743, Glyco_hydro_28 IPR006626, PbH1 IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence |
Pfami | View protein in Pfam PF00295, Glyco_hydro_28, 1 hit |
SMARTi | View protein in SMART SM00710, PbH1, 8 hits |
SUPFAMi | SSF51126, SSF51126, 1 hit |
PROSITEi | View protein in PROSITE PS00502, POLYGALACTURONASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q07181-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVRNIVSRLC SQLFALPSSS LQERDPCSVT EYSGLATAVS SCKNIVLNGF
60 70 80 90 100
QVPTGKQLDL SSLQNDSTVT FKGTTTFATT ADNDFNPIVI SGSNITITGA
110 120 130 140 150
SGHVIDGNGQ AYWDGKGSNS NSNQKPDHFI VVQKTTGNSK ITNLNIQNWP
160 170 180 190 200
VHCFDITGSS QLTISGLILD NRAGDKPNAK SGSLPAAHNT DGFDISSSDH
210 220 230 240 250
VTLDNNHVYN QDDCVAVTSG TNIVVSNMYC SGGHGLSIGS VGGKSDNVVD
260 270 280 290 300
GVQFLSSQVV NSQNGCRIKS NSGATGTINN VTYQNIALTN ISTYGVDVQQ
310 320 330 340 350
DYLNGGPTGK PTNGVKISNI KFIKVTGTVA SSAQDWFILC GDGSCSGFTF
360 370
SGNAITGGGK TSSCNYPTNT CPS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L02239 Genomic DNA Translation: AAA74586.1 |
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L02239 Genomic DNA Translation: AAA74586.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HG8 | X-ray | 1.73 | A | 25-373 | [»] | |
SMRi | Q07181 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 5127.CCT72952 |
Protein family/group databases
CAZyi | GH28, Glycoside Hydrolase Family 28 |
CLAEi | PGA28A_GIBMO |
Phylogenomic databases
eggNOGi | ENOG502QW1P, Eukaryota |
Enzyme and pathway databases
SABIO-RKi | Q07181 |
Miscellaneous databases
EvolutionaryTracei | Q07181 |
Family and domain databases
Gene3Di | 2.160.20.10, 1 hit |
InterProi | View protein in InterPro IPR000743, Glyco_hydro_28 IPR006626, PbH1 IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence |
Pfami | View protein in Pfam PF00295, Glyco_hydro_28, 1 hit |
SMARTi | View protein in SMART SM00710, PbH1, 8 hits |
SUPFAMi | SSF51126, SSF51126, 1 hit |
PROSITEi | View protein in PROSITE PS00502, POLYGALACTURONASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PGLR_GIBFU | |
Accessioni | Q07181Primary (citable) accession number: Q07181 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | August 12, 2020 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries