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Protein

Zinc finger protein Gfi-1

Gene

Gfi1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin-mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1-dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 promoter region. Implicated in the maintenance of inner ear hair cells (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri256 – 279C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri285 – 307C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri313 – 335C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri341 – 363C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri369 – 391C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri397 – 420C2H2-type 6PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein Gfi-1
Alternative name(s):
Growth factor independent protein 1
Gene namesi
Name:Gfi1
Synonyms:Gfi-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi2680 Gfi1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000471951 – 423Zinc finger protein Gfi-1Add BLAST423

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20PhosphoserineCombined sources1
Modified residuei57PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ07120
PRIDEiQ07120

PTM databases

iPTMnetiQ07120
PhosphoSitePlusiQ07120

Expressioni

Tissue specificityi

Restricted to lymphoid tissues and testes in adult animals.1 Publication

Developmental stagei

Expression enhanced late after interaction of IL-2 with its receptor, approximately when cells enter S phase.1 Publication

Gene expression databases

BgeeiENSRNOG00000002042 Expressed in 7 organ(s), highest expression level in colon
GenevisibleiQ07120 RN

Interactioni

Subunit structurei

Interacts with U2AF1L4. Component of RCOR-GFI-KDM1A-HDAC complexes. Interacts directly with RCOR1, KDM1A and HDAC2. Also interacts with HDAC1. regions. Interacts (via the zinc-finger domain) with ARIH2; the interaction prevents GFI1 ubiquitination and proteasomal degradation. Interacts with PIAS3; the interaction relieves the inhibitory effect of PIAS3 on STAT3-mediated transcriptional activity. Forms a complex with EHMT2 and HDAC1 to promote 'Lys-9' dimethylation of H3 (H3K9Me2) and repress expression of target genes. Interacts directly with EHMT2. Component of the GFI1-AJUBA-HDAC1 repressor complex. Interacts directly with AJUBA (via ITS LIM domains); the interaction results in the HDAC-dependent corepression of a subset of GFI1 target genes and, occurs independent of the SNAG domain. Interacts with SPI1; the interaction inhibits SPI1 transcriptional activity and represses SPI1-regulated macrophage-specific genes required for proper granulocyte development. Interacts with RUNX1T1; the interaction represses HDAC-mediated transcriptional activity. Interacts with RELA; the interaction occurs on liposaccharide (LPS) stimulation controls RELA DNA binding activity and regulates endotoxin-mediated TOLL-like receptor inflammatory response (By similarity). Interacts (via the C-terminal zinc fingers) with ZBTB17; the interaction results in the recruitment of GFI1 to the CDKN1A/p21 promoter and repression of CDKN1A/p21 transcription.By similarity2 Publications

Binary interactionsi

Protein-protein interaction databases

DIPiDIP-48689N
IntActiQ07120, 5 interactors
STRINGi10116.ENSRNOP00000002807

Structurei

Secondary structure

1423
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ07120
SMRiQ07120
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ07120

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 20SNAG domainBy similarityAdd BLAST20
Regioni141 – 258Required for interaction with RELABy similarityAdd BLAST118

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi159 – 210Ala/Gly-richAdd BLAST52

Domaini

Zinc fingers 3,4 and 5 are required for DNA binding and for interaction with SPI1.
The SNAG domain of GFIs is required for nuclear location and for interaction with some corepressors.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri256 – 279C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri285 – 307C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri313 – 335C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri341 – 363C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri369 – 391C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri397 – 420C2H2-type 6PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00740000115096
HOGENOMiHOG000067832
HOVERGENiHBG006967
InParanoidiQ07120
KOiK09223
OMAiPFSVPFK
OrthoDBiEOG091G0NDD
PhylomeDBiQ07120
TreeFamiTF350784

Family and domain databases

InterProiView protein in InterPro
IPR029840 GFI1/1B
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR44780 PTHR44780, 1 hit
PfamiView protein in Pfam
PF00096 zf-C2H2, 6 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 6 hits
SUPFAMiSSF57667 SSF57667, 3 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 6 hits
PS50157 ZINC_FINGER_C2H2_2, 6 hits

Sequencei

Sequence statusi: Complete.

Q07120-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPRSFLVKSK KAHSYHQPRS PGPDYSLRLE TVPVPGRADG GAVSAGESKM
60 70 80 90 100
EPRERLSPES QLTEAPDRAS ASPNSCEGSV CDPSSEFEDY WRPPSPSVSP
110 120 130 140 150
ASEKSLCRSL DEAQPYTLPF KPYAWSGLAG SDLRHLVQSY RQCSALERSA
160 170 180 190 200
GLSLFCERGA ESGRPAARYG SEQAAGGAGA GQPGSCGAAS GATSAGGLGL
210 220 230 240 250
YGDFAPAAAG LFERPSTAAG RLYQDRGHEL HADKSVGVKV ESELLCTRLL
260 270 280 290 300
LGGGSYKCIK CSKVFSTPHG LEVHVRRSHS GTRPFACEMC GKTFGHAVSL
310 320 330 340 350
EQHKAVHSQE RSFDCKICGK SFKRSSTLST HLLIHSDTRP YPCQYCGKRF
360 370 380 390 400
HQKSDMKKHT FIHTGEKPHK CQVCGKAFSQ SSNLITHSRK HTGFKPFGCD
410 420
LCGKGFQRKV DLRRHRETQH GLK
Length:423
Mass (Da):45,858
Last modified:October 1, 1994 - v1
Checksum:iFC39B6100D684D14
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06986 mRNA Translation: AAA41212.1
PIRiA48152
RefSeqiNP_036698.1, NM_012566.1
XP_008768139.1, XM_008769917.2
XP_008768140.1, XM_008769918.2
XP_017454563.1, XM_017599074.1
UniGeneiRn.10935

Genome annotation databases

EnsembliENSRNOT00000002807; ENSRNOP00000002807; ENSRNOG00000002042
GeneIDi24388
KEGGirno:24388
UCSCiRGD:2680 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06986 mRNA Translation: AAA41212.1
PIRiA48152
RefSeqiNP_036698.1, NM_012566.1
XP_008768139.1, XM_008769917.2
XP_008768140.1, XM_008769918.2
XP_017454563.1, XM_017599074.1
UniGeneiRn.10935

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KMKNMR-A312-393[»]
ProteinModelPortaliQ07120
SMRiQ07120
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48689N
IntActiQ07120, 5 interactors
STRINGi10116.ENSRNOP00000002807

PTM databases

iPTMnetiQ07120
PhosphoSitePlusiQ07120

Proteomic databases

PaxDbiQ07120
PRIDEiQ07120

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002807; ENSRNOP00000002807; ENSRNOG00000002042
GeneIDi24388
KEGGirno:24388
UCSCiRGD:2680 rat

Organism-specific databases

CTDi2672
RGDi2680 Gfi1

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00740000115096
HOGENOMiHOG000067832
HOVERGENiHBG006967
InParanoidiQ07120
KOiK09223
OMAiPFSVPFK
OrthoDBiEOG091G0NDD
PhylomeDBiQ07120
TreeFamiTF350784

Miscellaneous databases

EvolutionaryTraceiQ07120
PROiPR:Q07120

Gene expression databases

BgeeiENSRNOG00000002042 Expressed in 7 organ(s), highest expression level in colon
GenevisibleiQ07120 RN

Family and domain databases

InterProiView protein in InterPro
IPR029840 GFI1/1B
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR44780 PTHR44780, 1 hit
PfamiView protein in Pfam
PF00096 zf-C2H2, 6 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 6 hits
SUPFAMiSSF57667 SSF57667, 3 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 6 hits
PS50157 ZINC_FINGER_C2H2_2, 6 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGFI1_RAT
AccessioniPrimary (citable) accession number: Q07120
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 7, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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