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Protein

Growth/differentiation factor 3

Gene

Gdf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Growth factor involved in early embryonic development and adipose-tissue homeostasis. During embryogenesis controls formation of anterior visceral endoderm and mesoderm and the establishment of anterior-posterior identity through a receptor complex comprising the receptor ACVR1B and the coreceptor TDGF1/Cripto (PubMed:16368929, PubMed:17936261). Regulates adipose-tissue homeostasis and energy balance under nutrient overload in part by signaling through the receptor complex based on ACVR1C and TDGF1/Cripto.3 Publications

Miscellaneous

In contrast to other members of this family, cannot be disulfide-linked due to an atypical cysteine knot configuration, where the fourth cysteine is missing. This fourth cysteine is involved in an inter-molecular bridge to stabilize the active form of homodimeric or heterodimeric signaling molecules.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine, Developmental protein, Growth factor

Names & Taxonomyi

Protein namesi
Recommended name:
Growth/differentiation factor 3
Short name:
GDF-3
Alternative name(s):
VG-1-related protein 2
Gene namesi
Name:Gdf3
Synonyms:Gdf-3, Vgr-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:95686 Gdf3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Secreted

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality in one-third of the mutant embryos because of pregastrulation developmental malformations. However, the majority of mutants survive until adulthood without any overt abnormality (PubMed:17936261, PubMed:16368929). Mutant mice shown resistance to diet-induced obesity (PubMed:18480259).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000003391023 – 252Sequence analysisAdd BLAST230
ChainiPRO_0000033911253 – 366Growth/differentiation factor 3Add BLAST114

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi113N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi266 ↔ 331By similarity
Disulfide bondi295 ↔ 363By similarity
Disulfide bondi299 ↔ 365By similarity
Glycosylationi308N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Synthesized as large precursor molecule that undergo proteolytic cleavage, releasing the pro-domain from the active, receptor binding, C-terminal region of the molecule.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ07104
PeptideAtlasiQ07104
PRIDEiQ07104

PTM databases

iPTMnetiQ07104
PhosphoSitePlusiQ07104

Expressioni

Tissue specificityi

Primarily in adult bone marrow, spleen, thymus and adipose tissue.3 Publications

Inductioni

Markedly increased in an obese-mouse model lacking adipocyte fatty acid-binding protein FABP4.1 Publication

Gene expression databases

BgeeiENSMUSG00000030117 Expressed in 44 organ(s), highest expression level in embryo
CleanExiMM_GDF3
GenevisibleiQ07104 MM

Interactioni

Subunit structurei

Homodimer. Heterodimer (Potential). But, in contrast to other members of this family, cannot be disulfide-linked.Curated

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199887, 1 interactor
STRINGi10090.ENSMUSP00000032211

Structurei

3D structure databases

ProteinModelPortaliQ07104
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3900 Eukaryota
ENOG410XT8Z LUCA
GeneTreeiENSGT00910000143982
HOGENOMiHOG000249476
HOVERGENiHBG004860
InParanoidiQ07104
KOiK22672
OMAiANYCHGD
OrthoDBiEOG091G0K7Z
TreeFamiTF351789

Family and domain databases

Gene3Di2.10.90.10, 1 hit
InterProiView protein in InterPro
IPR029034 Cystine-knot_cytokine
IPR001839 TGF-b_C
IPR001111 TGF-b_propeptide
IPR015615 TGF-beta-rel
IPR017948 TGFb_CS
PANTHERiPTHR11848 PTHR11848, 1 hit
PfamiView protein in Pfam
PF00019 TGF_beta, 1 hit
PF00688 TGFb_propeptide, 1 hit
SMARTiView protein in SMART
SM00204 TGFB, 1 hit
SUPFAMiSSF57501 SSF57501, 1 hit
PROSITEiView protein in PROSITE
PS00250 TGF_BETA_1, 1 hit
PS51362 TGF_BETA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q07104-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQPYQRLLAL GFLLLTLPWG QTSEFQDSDL LQFLGLEKAP SPHRFQPVPR
60 70 80 90 100
VLRKIIRARE AAAASGASQD LCYVKELGVR GNLLQLLPDQ GFFLNTQKPF
110 120 130 140 150
QDGSCLQKVL YFNLSAIKEK AKLTMAQLTL DLGPRSYYNL RPELVVALSV
160 170 180 190 200
VQDRGVWGRS HPKVGRLLFL RSVPGPQGQL QFNLQGALKD WSSNRLKNLD
210 220 230 240 250
LHLEILVKED RYSRVTVQPE NPCDRLLRSL HASLLVVTLN PKHCHPSSRK
260 270 280 290 300
RRAAISVPKG FCRNFCHRHQ LFINFQDLGW HKWVIAPKGF MANYCHGECP
310 320 330 340 350
FSMTTYLNSS NYAFMQALMH MADPKVPKAV CVPTKLSPIS MLYQDSDKNV
360
ILRHYEDMVV DECGCG
Length:366
Mass (Da):41,586
Last modified:July 27, 2011 - v2
Checksum:i3DCD7CB8F9470293
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti165G → R in AAB24876 (PubMed:1480182).Curated1
Sequence conflicti167 – 168LL → FV in AAB24876 (PubMed:1480182).Curated2
Sequence conflicti225R → P in AAA53034 (PubMed:8429021).Curated1
Sequence conflicti225R → P in AAB24876 (PubMed:1480182).Curated1
Sequence conflicti313A → R in AAB24876 (PubMed:1480182).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06443 mRNA Translation: AAA53034.1
S52658 mRNA Translation: AAB24876.1
AK152457 mRNA Translation: BAE31235.1
AK160533 mRNA Translation: BAE35850.1
CCDSiCCDS20499.1
PIRiA45402
A46607
RefSeqiNP_032134.2, NM_008108.5
UniGeneiMm.299742

Genome annotation databases

EnsembliENSMUST00000032211; ENSMUSP00000032211; ENSMUSG00000030117
GeneIDi14562
KEGGimmu:14562
UCSCiuc009dpm.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06443 mRNA Translation: AAA53034.1
S52658 mRNA Translation: AAB24876.1
AK152457 mRNA Translation: BAE31235.1
AK160533 mRNA Translation: BAE35850.1
CCDSiCCDS20499.1
PIRiA45402
A46607
RefSeqiNP_032134.2, NM_008108.5
UniGeneiMm.299742

3D structure databases

ProteinModelPortaliQ07104
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199887, 1 interactor
STRINGi10090.ENSMUSP00000032211

PTM databases

iPTMnetiQ07104
PhosphoSitePlusiQ07104

Proteomic databases

PaxDbiQ07104
PeptideAtlasiQ07104
PRIDEiQ07104

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032211; ENSMUSP00000032211; ENSMUSG00000030117
GeneIDi14562
KEGGimmu:14562
UCSCiuc009dpm.1 mouse

Organism-specific databases

CTDi9573
MGIiMGI:95686 Gdf3

Phylogenomic databases

eggNOGiKOG3900 Eukaryota
ENOG410XT8Z LUCA
GeneTreeiENSGT00910000143982
HOGENOMiHOG000249476
HOVERGENiHBG004860
InParanoidiQ07104
KOiK22672
OMAiANYCHGD
OrthoDBiEOG091G0K7Z
TreeFamiTF351789

Miscellaneous databases

PROiPR:Q07104
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030117 Expressed in 44 organ(s), highest expression level in embryo
CleanExiMM_GDF3
GenevisibleiQ07104 MM

Family and domain databases

Gene3Di2.10.90.10, 1 hit
InterProiView protein in InterPro
IPR029034 Cystine-knot_cytokine
IPR001839 TGF-b_C
IPR001111 TGF-b_propeptide
IPR015615 TGF-beta-rel
IPR017948 TGFb_CS
PANTHERiPTHR11848 PTHR11848, 1 hit
PfamiView protein in Pfam
PF00019 TGF_beta, 1 hit
PF00688 TGFb_propeptide, 1 hit
SMARTiView protein in SMART
SM00204 TGFB, 1 hit
SUPFAMiSSF57501 SSF57501, 1 hit
PROSITEiView protein in PROSITE
PS00250 TGF_BETA_1, 1 hit
PS51362 TGF_BETA_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGDF3_MOUSE
AccessioniPrimary (citable) accession number: Q07104
Secondary accession number(s): Q3TUX1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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