UniProtKB - Q07104 (GDF3_MOUSE)
Protein
Growth/differentiation factor 3
Gene
Gdf3
Organism
Mus musculus (Mouse)
Status
Functioni
Growth factor involved in early embryonic development and adipose-tissue homeostasis. During embryogenesis controls formation of anterior visceral endoderm and mesoderm and the establishment of anterior-posterior identity through a receptor complex comprising the receptor ACVR1B and the coreceptor TDGF1/Cripto (PubMed:16368929, PubMed:17936261). Regulates adipose-tissue homeostasis and energy balance under nutrient overload in part by signaling through the receptor complex based on ACVR1C and TDGF1/Cripto.3 Publications
Miscellaneous
In contrast to other members of this family, cannot be disulfide-linked due to an atypical cysteine knot configuration, where the fourth cysteine is missing. This fourth cysteine is involved in an inter-molecular bridge to stabilize the active form of homodimeric or heterodimeric signaling molecules.By similarity
GO - Molecular functioni
- cytokine activity Source: GO_Central
- growth factor activity Source: UniProtKB-KW
- protein kinase binding Source: UniProtKB
GO - Biological processi
- BMP signaling pathway Source: GO_Central
- endoderm development Source: MGI
- eye development Source: UniProtKB
- formation of anatomical boundary Source: MGI
- in utero embryonic development Source: MGI
- mesoderm development Source: MGI
- negative regulation of BMP signaling pathway Source: UniProtKB
- negative regulation of epidermal cell differentiation Source: UniProtKB
- negative regulation of myoblast differentiation Source: MGI
- notochord development Source: MGI
- positive regulation of fat cell differentiation Source: BHF-UCL
- positive regulation of pathway-restricted SMAD protein phosphorylation Source: GO_Central
- primitive streak formation Source: MGI
- regulation of cell fate commitment Source: UniProtKB
- response to dietary excess Source: MGI
- signal transduction Source: MGI
- skeletal system development Source: UniProtKB
- SMAD protein signal transduction Source: GO_Central
- somite rostral/caudal axis specification Source: MGI
Keywordsi
Molecular function | Cytokine, Developmental protein, Growth factor |
Names & Taxonomyi
Protein namesi | Recommended name: Growth/differentiation factor 3Short name: GDF-3 Alternative name(s): VG-1-related protein 2 |
Gene namesi | Name:Gdf3 Synonyms:Gdf-3, Vgr-2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95686, Gdf3 |
Subcellular locationi
Extracellular region or secreted
- extracellular space Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, SecretedPathology & Biotechi
Disruption phenotypei
Embryonic lethality in one-third of the mutant embryos because of pregastrulation developmental malformations. However, the majority of mutants survive until adulthood without any overt abnormality (PubMed:17936261, PubMed:16368929). Mutant mice shown resistance to diet-induced obesity (PubMed:18480259).3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
PropeptideiPRO_0000033910 | 23 – 252 | Sequence analysisAdd BLAST | 230 | |
ChainiPRO_0000033911 | 253 – 366 | Growth/differentiation factor 3Add BLAST | 114 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 113 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 266 ↔ 331 | By similarity | ||
Disulfide bondi | 295 ↔ 363 | By similarity | ||
Disulfide bondi | 299 ↔ 365 | By similarity | ||
Glycosylationi | 308 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Synthesized as large precursor molecule that undergo proteolytic cleavage, releasing the pro-domain from the active, receptor binding, C-terminal region of the molecule.By similarity
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q07104 |
PeptideAtlasi | Q07104 |
PRIDEi | Q07104 |
PTM databases
GlyGeni | Q07104, 2 sites |
iPTMneti | Q07104 |
PhosphoSitePlusi | Q07104 |
Expressioni
Tissue specificityi
Primarily in adult bone marrow, spleen, thymus and adipose tissue.3 Publications
Inductioni
Markedly increased in an obese-mouse model lacking adipocyte fatty acid-binding protein FABP4.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000030117, Expressed in stomach and 67 other tissues |
Genevisiblei | Q07104, MM |
Interactioni
Subunit structurei
Homodimer. Heterodimer (Potential). But, in contrast to other members of this family, cannot be disulfide-linked.
CuratedGO - Molecular functioni
- cytokine activity Source: GO_Central
- growth factor activity Source: UniProtKB-KW
- protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 199887, 1 interactor |
STRINGi | 10090.ENSMUSP00000032211 |
Miscellaneous databases
RNActi | Q07104, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the TGF-beta family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3900, Eukaryota |
GeneTreei | ENSGT00940000162534 |
HOGENOMi | CLU_020515_4_0_1 |
InParanoidi | Q07104 |
OMAi | QKFQPVP |
OrthoDBi | 919690at2759 |
TreeFami | TF351789 |
Family and domain databases
Gene3Di | 2.10.90.10, 1 hit |
InterProi | View protein in InterPro IPR029034, Cystine-knot_cytokine IPR001839, TGF-b_C IPR015615, TGF-beta-rel IPR017948, TGFb_CS |
PANTHERi | PTHR11848, PTHR11848, 1 hit |
Pfami | View protein in Pfam PF00019, TGF_beta, 1 hit |
SMARTi | View protein in SMART SM00204, TGFB, 1 hit |
SUPFAMi | SSF57501, SSF57501, 1 hit |
PROSITEi | View protein in PROSITE PS00250, TGF_BETA_1, 1 hit PS51362, TGF_BETA_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q07104-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQPYQRLLAL GFLLLTLPWG QTSEFQDSDL LQFLGLEKAP SPHRFQPVPR
60 70 80 90 100
VLRKIIRARE AAAASGASQD LCYVKELGVR GNLLQLLPDQ GFFLNTQKPF
110 120 130 140 150
QDGSCLQKVL YFNLSAIKEK AKLTMAQLTL DLGPRSYYNL RPELVVALSV
160 170 180 190 200
VQDRGVWGRS HPKVGRLLFL RSVPGPQGQL QFNLQGALKD WSSNRLKNLD
210 220 230 240 250
LHLEILVKED RYSRVTVQPE NPCDRLLRSL HASLLVVTLN PKHCHPSSRK
260 270 280 290 300
RRAAISVPKG FCRNFCHRHQ LFINFQDLGW HKWVIAPKGF MANYCHGECP
310 320 330 340 350
FSMTTYLNSS NYAFMQALMH MADPKVPKAV CVPTKLSPIS MLYQDSDKNV
360
ILRHYEDMVV DECGCG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 165 | G → R in AAB24876 (PubMed:1480182).Curated | 1 | |
Sequence conflicti | 167 – 168 | LL → FV in AAB24876 (PubMed:1480182).Curated | 2 | |
Sequence conflicti | 225 | R → P in AAA53034 (PubMed:8429021).Curated | 1 | |
Sequence conflicti | 225 | R → P in AAB24876 (PubMed:1480182).Curated | 1 | |
Sequence conflicti | 313 | A → R in AAB24876 (PubMed:1480182).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06443 mRNA Translation: AAA53034.1 S52658 mRNA Translation: AAB24876.1 AK152457 mRNA Translation: BAE31235.1 AK160533 mRNA Translation: BAE35850.1 |
CCDSi | CCDS20499.1 |
PIRi | A45402 A46607 |
RefSeqi | NP_032134.2, NM_008108.5 |
Genome annotation databases
Ensembli | ENSMUST00000032211; ENSMUSP00000032211; ENSMUSG00000030117 |
GeneIDi | 14562 |
KEGGi | mmu:14562 |
UCSCi | uc009dpm.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06443 mRNA Translation: AAA53034.1 S52658 mRNA Translation: AAB24876.1 AK152457 mRNA Translation: BAE31235.1 AK160533 mRNA Translation: BAE35850.1 |
CCDSi | CCDS20499.1 |
PIRi | A45402 A46607 |
RefSeqi | NP_032134.2, NM_008108.5 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 199887, 1 interactor |
STRINGi | 10090.ENSMUSP00000032211 |
PTM databases
GlyGeni | Q07104, 2 sites |
iPTMneti | Q07104 |
PhosphoSitePlusi | Q07104 |
Proteomic databases
PaxDbi | Q07104 |
PeptideAtlasi | Q07104 |
PRIDEi | Q07104 |
Protocols and materials databases
Antibodypediai | 11440, 407 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000032211; ENSMUSP00000032211; ENSMUSG00000030117 |
GeneIDi | 14562 |
KEGGi | mmu:14562 |
UCSCi | uc009dpm.1, mouse |
Organism-specific databases
CTDi | 9573 |
MGIi | MGI:95686, Gdf3 |
Phylogenomic databases
eggNOGi | KOG3900, Eukaryota |
GeneTreei | ENSGT00940000162534 |
HOGENOMi | CLU_020515_4_0_1 |
InParanoidi | Q07104 |
OMAi | QKFQPVP |
OrthoDBi | 919690at2759 |
TreeFami | TF351789 |
Miscellaneous databases
BioGRID-ORCSi | 14562, 0 hits in 17 CRISPR screens |
PROi | PR:Q07104 |
RNActi | Q07104, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030117, Expressed in stomach and 67 other tissues |
Genevisiblei | Q07104, MM |
Family and domain databases
Gene3Di | 2.10.90.10, 1 hit |
InterProi | View protein in InterPro IPR029034, Cystine-knot_cytokine IPR001839, TGF-b_C IPR015615, TGF-beta-rel IPR017948, TGFb_CS |
PANTHERi | PTHR11848, PTHR11848, 1 hit |
Pfami | View protein in Pfam PF00019, TGF_beta, 1 hit |
SMARTi | View protein in SMART SM00204, TGFB, 1 hit |
SUPFAMi | SSF57501, SSF57501, 1 hit |
PROSITEi | View protein in PROSITE PS00250, TGF_BETA_1, 1 hit PS51362, TGF_BETA_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GDF3_MOUSE | |
Accessioni | Q07104Primary (citable) accession number: Q07104 Secondary accession number(s): Q3TUX1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families