UniProtKB - Q07084 (SSK1_YEAST)
Protein
Osmolarity two-component system protein SSK1
Gene
SSK1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Final receptor of the SLN1-YPD1-SSK1 two-component regulatory system, which controls activity of the HOG1 pathway in response to changes in the osmolarity of the extracellular environment. Under normal osmotic conditions, maintained in a phosphorylated and inactive state by the phosphorelay intermediate protein YPD1. Under conditions of high osmolarity, the histidine kinase SLN1 is no longer active and the unphosphorylated form of SSK1 interacts with and activates SSK2 and SSK22, two MAPKKKs that further stimulate the PBS2-HOG1 MAPKK-MAPK cascade. Unphosphorylated SSK1 is subsequently degraded by the UBC7-dependent ubiquitin-proteasome system to down-regulate the HOG1 pathway after completion of the osmotic adaptation.3 Publications
Miscellaneous
Present with 1200 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- mitogen-activated protein kinase kinase kinase binding Source: SGD
- phosphorelay response regulator activity Source: SGD
- protein kinase activator activity Source: SGD
GO - Biological processi
- activation of MAPKKK activity Source: SGD
- osmosensory signaling via phosphorelay pathway Source: SGD
- positive regulation of actin cytoskeleton reorganization Source: SGD
- positive regulation of protein autophosphorylation Source: SGD
Keywordsi
Biological process | Two-component regulatory system |
Names & Taxonomyi
Protein namesi | Recommended name: Osmolarity two-component system protein SSK1 |
Gene namesi | Name:SSK1 Ordered Locus Names:YLR006C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003996, SSK1 |
VEuPathDBi | FungiDB:YLR006C |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: SGD
- Mcs4 RR-MAPKKK complex Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 554 | D → N: Activates. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081406 | 1 – 712 | Osmolarity two-component system protein SSK1Add BLAST | 712 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 110 | PhosphoserineCombined sources | 1 | |
Modified residuei | 195 | PhosphoserineCombined sources | 1 | |
Modified residuei | 327 | PhosphoserineCombined sources | 1 | |
Modified residuei | 351 | PhosphoserineCombined sources | 1 | |
Modified residuei | 368 | PhosphoserineCombined sources | 1 | |
Modified residuei | 380 | PhosphoserineCombined sources | 1 | |
Modified residuei | 554 | 4-aspartylphosphatePROSITE-ProRule annotation1 Publication | 1 | |
Modified residuei | 673 | PhosphoserineCombined sources | 1 | |
Modified residuei | 693 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 703 | PhosphoserineCombined sources | 1 | |
Modified residuei | 706 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
The phosphorelay mechanism involves the sequential transfer of a phosphate group from 'His-576' (H1) to 'Asp-1144' (D1) of SLN1, then to 'His-64' (H2) of YPD1 and finally to Asp-554 (D2) of SSK1.
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q07084 |
PaxDbi | Q07084 |
PRIDEi | Q07084 |
PTM databases
iPTMneti | Q07084 |
Interactioni
Subunit structurei
Interacts with SSK2, SSK22 and YPD1.
1 PublicationBinary interactionsi
Hide detailsQ07084
With | #Exp. | IntAct |
---|---|---|
SSK2 [P53599] | 7 | EBI-18184,EBI-18191 |
SSK22 [P25390] | 5 | EBI-18184,EBI-18129 |
YPD1 [Q07688] | 3 | EBI-18184,EBI-34423 |
GO - Molecular functioni
Protein-protein interaction databases
BioGRIDi | 31279, 135 interactors |
DIPi | DIP-2437N |
IntActi | Q07084, 42 interactors |
MINTi | Q07084 |
STRINGi | 4932.YLR006C |
Miscellaneous databases
RNActi | Q07084, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q07084 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 505 – 647 | Response regulatoryPROSITE-ProRule annotationAdd BLAST | 143 |
Phylogenomic databases
eggNOGi | KOG0519, Eukaryota |
HOGENOMi | CLU_008307_3_0_1 |
InParanoidi | Q07084 |
OMAi | NEDTITH |
Family and domain databases
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR001789, Sig_transdc_resp-reg_receiver |
Pfami | View protein in Pfam PF00072, Response_reg, 1 hit |
SMARTi | View protein in SMART SM00448, REC, 1 hit |
SUPFAMi | SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS50110, RESPONSE_REGULATORY, 1 hit |
i Sequence
Sequence statusi: Complete.
Q07084-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLNSALLWKV WLRIDNSTDE VNQPIAVQFD EIDTVDDLKS RFFQKLSSTR
60 70 80 90 100
WREINDNASI AIGLYAPKFD NQADNTSSNN TNDNSCRSKS NGAGSGANLS
110 120 130 140 150
VNSNTKSSVS PTAGSFGLSK DLAKDRNVLQ HPKPTQKRGA LYDAFAAVPT
160 170 180 190 200
VAATTNVDFP PNEAPMLSPQ RPYSTSPKQF PATTKSPLLR FASVSPYPKF
210 220 230 240 250
HSDNQIMASA GLTYVSPHNK NKYTRPLIRK GLNFTTESVN DCTYKIIFEP
260 270 280 290 300
DELAINIYKE LFGTMGSQPA SQPLLIFSNV NLRQDVPPLD ILNVVDYVPT
310 320 330 340 350
NEEISQQKTQ PTDHGAVGVF HLDDHISPGE QGLKQTIGDK ADLKGKDGNS
360 370 380 390 400
SPQEFKLITD EEQLRRASQE LKDEEKDAES PWQAILLLPK GYKGGVDFRN
410 420 430 440 450
KPVAHTDSSF NNEDTITHSE LEVNTGSPSQ ESGSLNEAGI GITQPMSEVQ
460 470 480 490 500
RRKEDVTPAS PILTSSQTPH YSNSLYNAPF AVSSPPDPLP NLFTTTSEKV
510 520 530 540 550
FPKINVLIVE DNVINQAILG SFLRKHKISY KLAKNGQEAV NIWKEGGLHL
560 570 580 590 600
IFMDLQLPVL SGIEAAKQIR DFEKQNGIGI QKSLNNSHSN LEKGTSKRFS
610 620 630 640 650
QAPVIIVALT ASNSQMDKRK ALLSGCNDYL TKPVNLHWLS KKITEWGCMQ
660 670 680 690 700
ALIDFDSWKQ GESRMTDSVL VKSPQKPIAP SNPHSFKQAT SMTPTHSPVR
710
KNSNLSPTQI EL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 181 | P → S in AAA35100 (PubMed:8183345).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26523 Genomic DNA Translation: AAA35100.1 Z73178 Genomic DNA Translation: CAA97528.1 BK006945 Genomic DNA Translation: DAA09324.1 |
PIRi | S64828 |
RefSeqi | NP_013106.1, NM_001181893.1 |
Genome annotation databases
EnsemblFungii | YLR006C_mRNA; YLR006C; YLR006C |
GeneIDi | 850692 |
KEGGi | sce:YLR006C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L26523 Genomic DNA Translation: AAA35100.1 Z73178 Genomic DNA Translation: CAA97528.1 BK006945 Genomic DNA Translation: DAA09324.1 |
PIRi | S64828 |
RefSeqi | NP_013106.1, NM_001181893.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5KBX | X-ray | 2.80 | B | 495-712 | [»] | |
SMRi | Q07084 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31279, 135 interactors |
DIPi | DIP-2437N |
IntActi | Q07084, 42 interactors |
MINTi | Q07084 |
STRINGi | 4932.YLR006C |
PTM databases
iPTMneti | Q07084 |
Proteomic databases
MaxQBi | Q07084 |
PaxDbi | Q07084 |
PRIDEi | Q07084 |
Genome annotation databases
EnsemblFungii | YLR006C_mRNA; YLR006C; YLR006C |
GeneIDi | 850692 |
KEGGi | sce:YLR006C |
Organism-specific databases
SGDi | S000003996, SSK1 |
VEuPathDBi | FungiDB:YLR006C |
Phylogenomic databases
eggNOGi | KOG0519, Eukaryota |
HOGENOMi | CLU_008307_3_0_1 |
InParanoidi | Q07084 |
OMAi | NEDTITH |
Miscellaneous databases
PROi | PR:Q07084 |
RNActi | Q07084, protein |
Family and domain databases
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR001789, Sig_transdc_resp-reg_receiver |
Pfami | View protein in Pfam PF00072, Response_reg, 1 hit |
SMARTi | View protein in SMART SM00448, REC, 1 hit |
SUPFAMi | SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS50110, RESPONSE_REGULATORY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SSK1_YEAST | |
Accessioni | Q07084Primary (citable) accession number: Q07084 Secondary accession number(s): D6VY08, Q07909 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references