UniProtKB - Q07065 (CKAP4_HUMAN)
Protein
Cytoskeleton-associated protein 4
Gene
CKAP4
Organism
Homo sapiens (Human)
Status
Functioni
Mediates the anchoring of the endoplasmic reticulum to microtubules.1 Publication
High-affinity epithelial cell surface receptor for the FZD8-related low molecular weight sialoglycopeptide APF/antiproliferative factor. Mediates the APF antiproliferative signaling within cells.2 Publications
GO - Molecular functioni
- RNA binding Source: UniProtKB
GO - Biological processi
- cellular protein metabolic process Source: Reactome
- neutrophil degranulation Source: Reactome
- post-translational protein modification Source: Reactome
Enzyme and pathway databases
PathwayCommonsi | Q07065 |
Reactomei | R-HSA-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-HSA-5683826, Surfactant metabolism R-HSA-6798695, Neutrophil degranulation R-HSA-8957275, Post-translational protein phosphorylation |
Names & Taxonomyi
Protein namesi | Recommended name: Cytoskeleton-associated protein 4Alternative name(s): 63-kDa cytoskeleton-linking membrane protein Short name: Climp-63 Short name: p63 |
Gene namesi | Name:CKAP4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16991, CKAP4 |
MIMi | 618595, gene |
neXtProti | NX_Q07065 |
VEuPathDBi | HostDB:ENSG00000136026.13 |
Subcellular locationi
Cytoskeleton
Plasma membrane
- Cell membrane 2 Publications; Single-pass type II membrane protein
Endoplasmic reticulum
- Endoplasmic reticulum membrane 2 Publications; Single-pass type II membrane protein
Other locations
Note: Translocates to the perinuclear region upon APF-stimulation.
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Cytosol
- cytosol Source: HPA
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum lumen Source: Reactome
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
- rough endoplasmic reticulum Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Lysosome
- azurophil granule membrane Source: Reactome
Nucleus
- nuclear speck Source: HPA
Plasma Membrane
- plasma membrane Source: Reactome
Other locations
- cytoplasmic ribonucleoprotein granule Source: HPA
- integral component of membrane Source: ProtInc
- lamellar body Source: Reactome
- lipid droplet Source: UniProtKB
- membrane Source: ProtInc
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- specific granule membrane Source: Reactome
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 106 | CytoplasmicSequence analysisAdd BLAST | 106 | |
Transmembranei | 107 – 127 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 128 – 602 | ExtracellularSequence analysisAdd BLAST | 475 |
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 10970 |
OpenTargetsi | ENSG00000136026 |
PharmGKBi | PA26527 |
Miscellaneous databases
Pharosi | Q07065, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4296025 |
Genetic variation databases
BioMutai | CKAP4 |
DMDMi | 74735614 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000252417 | 1 – 602 | Cytoskeleton-associated protein 4Add BLAST | 602 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 3 | Phosphoserine1 Publication | 1 | |
Modified residuei | 17 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 19 | Phosphoserine1 Publication | 1 | |
Modified residuei | 21 | N6-acetyllysineBy similarity | 1 | |
Lipidationi | 100 | S-palmitoyl cysteine; by ZDHHC22 Publications | 1 | |
Modified residuei | 232 | Phosphoserine; by FAM20C1 Publication | 1 | |
Modified residuei | 312 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Reversibly palmitoylated. Palmitoylation at Cys-100 by ZDHHC2 is required for its trafficking from the ER to the plasma membrane and for its perinuclear localization. Palmitoylation by ZDHHC2 is also required for its function in APF-mediated antiproliferative signaling (PubMed:19144824).3 Publications
Increased phosphorylation during mitosis prevents binding to microtubules.1 Publication
Keywords - PTMi
Acetylation, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
CPTACi | CPTAC-335 CPTAC-336 |
EPDi | Q07065 |
jPOSTi | Q07065 |
MassIVEi | Q07065 |
MaxQBi | Q07065 |
PaxDbi | Q07065 |
PeptideAtlasi | Q07065 |
PRIDEi | Q07065 |
ProteomicsDBi | 58501 |
PTM databases
GlyGeni | Q07065, 2 sites |
iPTMneti | Q07065 |
PhosphoSitePlusi | Q07065 |
SwissPalmi | Q07065 |
Expressioni
Gene expression databases
Bgeei | ENSG00000136026, Expressed in tibia and 254 other tissues |
ExpressionAtlasi | Q07065, baseline and differential |
Genevisiblei | Q07065, HS |
Organism-specific databases
HPAi | ENSG00000136026, Low tissue specificity |
Interactioni
Protein-protein interaction databases
BioGRIDi | 116167, 198 interactors |
IntActi | Q07065, 122 interactors |
MINTi | Q07065 |
STRINGi | 9606.ENSP00000367265 |
Miscellaneous databases
RNActi | Q07065, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 130 – 214 | Sequence analysisAdd BLAST | 85 | |
Coiled coili | 256 – 460 | Sequence analysisAdd BLAST | 205 | |
Coiled coili | 533 – 602 | Sequence analysisAdd BLAST | 70 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 36 – 59 | Pro-richAdd BLAST | 24 | |
Compositional biasi | 71 – 77 | Poly-Gly | 7 | |
Compositional biasi | 85 – 94 | Poly-Ala | 10 | |
Compositional biasi | 118 – 121 | Poly-Ala | 4 |
Keywords - Domaini
Coiled coil, Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QVCP, Eukaryota |
GeneTreei | ENSGT00390000015968 |
HOGENOMi | CLU_032481_0_0_1 |
InParanoidi | Q07065 |
OMAi | SEGYKQD |
OrthoDBi | 1255457at2759 |
PhylomeDBi | Q07065 |
TreeFami | TF332395 |
Family and domain databases
InterProi | View protein in InterPro IPR028428, Ckap4 |
PANTHERi | PTHR45161, PTHR45161, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q07065-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPSAKQRGSK GGHGAASPSE KGAHPSGGAD DVAKKPPPAP QQPPPPPAPH
60 70 80 90 100
PQQHPQQHPQ NQAHGKGGHR GGGGGGGKSS SSSSASAAAA AAAASSSASC
110 120 130 140 150
SRRLGRALNF LFYLALVAAA AFSGWCVHHV LEEVQQVRRS HQDFSRQREE
160 170 180 190 200
LGQGLQGVEQ KVQSLQATFG TFESILRSSQ HKQDLTEKAV KQGESEVSRI
210 220 230 240 250
SEVLQKLQNE ILKDLSDGIH VVKDARERDF TSLENTVEER LTELTKSIND
260 270 280 290 300
NIAIFTEVQK RSQKEINDMK AKVASLEESE GNKQDLKALK EAVKEIQTSA
310 320 330 340 350
KSREWDMEAL RSTLQTMESD IYTEVRELVS LKQEQQAFKE AADTERLALQ
360 370 380 390 400
ALTEKLLRSE ESVSRLPEEI RRLEEELRQL KSDSHGPKED GGFRHSEAFE
410 420 430 440 450
ALQQKSQGLD SRLQHVEDGV LSMQVASARQ TESLESLLSK SQEHEQRLAA
460 470 480 490 500
LQGRLEGLGS SEADQDGLAS TVRSLGETQL VLYGDVEELK RSVGELPSTV
510 520 530 540 550
ESLQKVQEQV HTLLSQDQAQ AARLPPQDFL DRLSSLDNLK ASVSQVEADL
560 570 580 590 600
KMLRTAVDSL VAYSVKIETN ENNLESAKGL LDDLRNDLDR LFVKVEKIHE
KV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF8VVU0 | F8VVU0_HUMAN | Cytoskeleton-associated protein 4 | CKAP4 | 26 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 60 | Q → R in BAD97283 (Ref. 2) Curated | 1 | |
Sequence conflicti | 362 | S → P in AAH94824 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_027853 | 348 | A → T. Corresponds to variant dbSNP:rs3088113Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69910 mRNA Translation: CAA49535.2 AK223563 mRNA Translation: BAD97283.1 AC079174 Genomic DNA No translation available. BC082972 mRNA Translation: AAH82972.1 BC094824 mRNA Translation: AAH94824.1 Sequence problems. |
CCDSi | CCDS9103.1 |
PIRi | S33377 |
RefSeqi | NP_006816.2, NM_006825.3 |
Genome annotation databases
Ensembli | ENST00000378026; ENSP00000367265; ENSG00000136026 |
GeneIDi | 10970 |
KEGGi | hsa:10970 |
UCSCi | uc001tlk.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69910 mRNA Translation: CAA49535.2 AK223563 mRNA Translation: BAD97283.1 AC079174 Genomic DNA No translation available. BC082972 mRNA Translation: AAH82972.1 BC094824 mRNA Translation: AAH94824.1 Sequence problems. |
CCDSi | CCDS9103.1 |
PIRi | S33377 |
RefSeqi | NP_006816.2, NM_006825.3 |
3D structure databases
SMRi | Q07065 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 116167, 198 interactors |
IntActi | Q07065, 122 interactors |
MINTi | Q07065 |
STRINGi | 9606.ENSP00000367265 |
Chemistry databases
ChEMBLi | CHEMBL4296025 |
PTM databases
GlyGeni | Q07065, 2 sites |
iPTMneti | Q07065 |
PhosphoSitePlusi | Q07065 |
SwissPalmi | Q07065 |
Genetic variation databases
BioMutai | CKAP4 |
DMDMi | 74735614 |
Proteomic databases
CPTACi | CPTAC-335 CPTAC-336 |
EPDi | Q07065 |
jPOSTi | Q07065 |
MassIVEi | Q07065 |
MaxQBi | Q07065 |
PaxDbi | Q07065 |
PeptideAtlasi | Q07065 |
PRIDEi | Q07065 |
ProteomicsDBi | 58501 |
Protocols and materials databases
Antibodypediai | 613, 274 antibodies |
Genome annotation databases
Ensembli | ENST00000378026; ENSP00000367265; ENSG00000136026 |
GeneIDi | 10970 |
KEGGi | hsa:10970 |
UCSCi | uc001tlk.4, human |
Organism-specific databases
CTDi | 10970 |
DisGeNETi | 10970 |
GeneCardsi | CKAP4 |
HGNCi | HGNC:16991, CKAP4 |
HPAi | ENSG00000136026, Low tissue specificity |
MIMi | 618595, gene |
neXtProti | NX_Q07065 |
OpenTargetsi | ENSG00000136026 |
PharmGKBi | PA26527 |
VEuPathDBi | HostDB:ENSG00000136026.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QVCP, Eukaryota |
GeneTreei | ENSGT00390000015968 |
HOGENOMi | CLU_032481_0_0_1 |
InParanoidi | Q07065 |
OMAi | SEGYKQD |
OrthoDBi | 1255457at2759 |
PhylomeDBi | Q07065 |
TreeFami | TF332395 |
Enzyme and pathway databases
PathwayCommonsi | Q07065 |
Reactomei | R-HSA-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-HSA-5683826, Surfactant metabolism R-HSA-6798695, Neutrophil degranulation R-HSA-8957275, Post-translational protein phosphorylation |
Miscellaneous databases
BioGRID-ORCSi | 10970, 4 hits in 877 CRISPR screens |
ChiTaRSi | CKAP4, human |
GeneWikii | CKAP4 |
GenomeRNAii | 10970 |
Pharosi | Q07065, Tbio |
PROi | PR:Q07065 |
RNActi | Q07065, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000136026, Expressed in tibia and 254 other tissues |
ExpressionAtlasi | Q07065, baseline and differential |
Genevisiblei | Q07065, HS |
Family and domain databases
InterProi | View protein in InterPro IPR028428, Ckap4 |
PANTHERi | PTHR45161, PTHR45161, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CKAP4_HUMAN | |
Accessioni | Q07065Primary (citable) accession number: Q07065 Secondary accession number(s): Q504S5, Q53ES6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | June 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot