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Protein

Cytoskeleton-associated protein 4

Gene

CKAP4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

High-affinity epithelial cell surface receptor for APF.
Mediates the anchoring of the endoplasmic reticulum to microtubules.

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-HSA-5683826 Surfactant metabolism
R-HSA-6798695 Neutrophil degranulation
R-HSA-8957275 Post-translational protein phosphorylation

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoskeleton-associated protein 4
Alternative name(s):
63-kDa cytoskeleton-linking membrane protein
Short name:
Climp-63
Short name:
p63
Gene namesi
Name:CKAP4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000136026.13
HGNCiHGNC:16991 CKAP4
neXtProtiNX_Q07065

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 106CytoplasmicSequence analysisAdd BLAST106
Transmembranei107 – 127HelicalSequence analysisAdd BLAST21
Topological domaini128 – 602ExtracellularSequence analysisAdd BLAST475

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10970
OpenTargetsiENSG00000136026
PharmGKBiPA26527

Polymorphism and mutation databases

BioMutaiCKAP4
DMDMi74735614

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002524171 – 602Cytoskeleton-associated protein 4Add BLAST602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3Phosphoserine1 Publication1
Modified residuei17PhosphoserineCombined sources1 Publication1
Modified residuei19Phosphoserine1 Publication1
Modified residuei21N6-acetyllysineBy similarity1
Lipidationi100S-palmitoyl cysteine; by ZDHHC21 Publication1
Modified residuei232Phosphoserine; by FAM20C1 Publication1
Modified residuei312PhosphoserineCombined sources1

Post-translational modificationi

Reversibly palmitoylated. Palmitoylation at Cys-100 by DHHC2 is required for its trafficking from the ER to the plasma membrane and for its perinuclear localization.2 Publications
Increased phosphorylation during mitosis prevents binding to microtubules.1 Publication

Keywords - PTMi

Acetylation, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ07065
MaxQBiQ07065
PaxDbiQ07065
PeptideAtlasiQ07065
PRIDEiQ07065
ProteomicsDBi58501

PTM databases

iPTMnetiQ07065
PhosphoSitePlusiQ07065
SwissPalmiQ07065

Expressioni

Gene expression databases

BgeeiENSG00000136026 Expressed in 243 organ(s), highest expression level in tibia
CleanExiHS_CKAP4
ExpressionAtlasiQ07065 baseline and differential
GenevisibleiQ07065 HS

Organism-specific databases

HPAiHPA000278
HPA000792
HPA001225

Interactioni

Protein-protein interaction databases

BioGridi116167, 107 interactors
IntActiQ07065, 63 interactors
MINTiQ07065
STRINGi9606.ENSP00000367265

Structurei

3D structure databases

ProteinModelPortaliQ07065
SMRiQ07065
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili130 – 214Sequence analysisAdd BLAST85
Coiled coili256 – 460Sequence analysisAdd BLAST205
Coiled coili533 – 602Sequence analysisAdd BLAST70

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi36 – 59Pro-richAdd BLAST24
Compositional biasi71 – 77Poly-Gly7
Compositional biasi85 – 94Poly-Ala10
Compositional biasi118 – 121Poly-Ala4

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIRS Eukaryota
ENOG4111Q54 LUCA
GeneTreeiENSGT00390000015968
HOGENOMiHOG000060146
HOVERGENiHBG101940
InParanoidiQ07065
KOiK13999
OMAiHQDFSRQ
OrthoDBiEOG091G067G
PhylomeDBiQ07065
TreeFamiTF332395

Family and domain databases

InterProiView protein in InterPro
IPR028428 Ckap4
PANTHERiPTHR45161 PTHR45161, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q07065-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPSAKQRGSK GGHGAASPSE KGAHPSGGAD DVAKKPPPAP QQPPPPPAPH
60 70 80 90 100
PQQHPQQHPQ NQAHGKGGHR GGGGGGGKSS SSSSASAAAA AAAASSSASC
110 120 130 140 150
SRRLGRALNF LFYLALVAAA AFSGWCVHHV LEEVQQVRRS HQDFSRQREE
160 170 180 190 200
LGQGLQGVEQ KVQSLQATFG TFESILRSSQ HKQDLTEKAV KQGESEVSRI
210 220 230 240 250
SEVLQKLQNE ILKDLSDGIH VVKDARERDF TSLENTVEER LTELTKSIND
260 270 280 290 300
NIAIFTEVQK RSQKEINDMK AKVASLEESE GNKQDLKALK EAVKEIQTSA
310 320 330 340 350
KSREWDMEAL RSTLQTMESD IYTEVRELVS LKQEQQAFKE AADTERLALQ
360 370 380 390 400
ALTEKLLRSE ESVSRLPEEI RRLEEELRQL KSDSHGPKED GGFRHSEAFE
410 420 430 440 450
ALQQKSQGLD SRLQHVEDGV LSMQVASARQ TESLESLLSK SQEHEQRLAA
460 470 480 490 500
LQGRLEGLGS SEADQDGLAS TVRSLGETQL VLYGDVEELK RSVGELPSTV
510 520 530 540 550
ESLQKVQEQV HTLLSQDQAQ AARLPPQDFL DRLSSLDNLK ASVSQVEADL
560 570 580 590 600
KMLRTAVDSL VAYSVKIETN ENNLESAKGL LDDLRNDLDR LFVKVEKIHE

KV
Length:602
Mass (Da):66,022
Last modified:June 1, 2002 - v2
Checksum:i216E6A92ED5E058A
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VVU0F8VVU0_HUMAN
Cytoskeleton-associated protein 4
CKAP4
26Annotation score:

Sequence cautioni

The sequence AAH94824 differs from that shown. Aberrant splicing.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60Q → R in BAD97283 (Ref. 2) Curated1
Sequence conflicti362S → P in AAH94824 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027853348A → T. Corresponds to variant dbSNP:rs3088113Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69910 mRNA Translation: CAA49535.2
AK223563 mRNA Translation: BAD97283.1
AC079174 Genomic DNA No translation available.
BC082972 mRNA Translation: AAH82972.1
BC094824 mRNA Translation: AAH94824.1 Sequence problems.
CCDSiCCDS9103.1
PIRiS33377
RefSeqiNP_006816.2, NM_006825.3
UniGeneiHs.74368

Genome annotation databases

EnsembliENST00000378026; ENSP00000367265; ENSG00000136026
GeneIDi10970
KEGGihsa:10970
UCSCiuc001tlk.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69910 mRNA Translation: CAA49535.2
AK223563 mRNA Translation: BAD97283.1
AC079174 Genomic DNA No translation available.
BC082972 mRNA Translation: AAH82972.1
BC094824 mRNA Translation: AAH94824.1 Sequence problems.
CCDSiCCDS9103.1
PIRiS33377
RefSeqiNP_006816.2, NM_006825.3
UniGeneiHs.74368

3D structure databases

ProteinModelPortaliQ07065
SMRiQ07065
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116167, 107 interactors
IntActiQ07065, 63 interactors
MINTiQ07065
STRINGi9606.ENSP00000367265

PTM databases

iPTMnetiQ07065
PhosphoSitePlusiQ07065
SwissPalmiQ07065

Polymorphism and mutation databases

BioMutaiCKAP4
DMDMi74735614

Proteomic databases

EPDiQ07065
MaxQBiQ07065
PaxDbiQ07065
PeptideAtlasiQ07065
PRIDEiQ07065
ProteomicsDBi58501

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000378026; ENSP00000367265; ENSG00000136026
GeneIDi10970
KEGGihsa:10970
UCSCiuc001tlk.4 human

Organism-specific databases

CTDi10970
DisGeNETi10970
EuPathDBiHostDB:ENSG00000136026.13
GeneCardsiCKAP4
HGNCiHGNC:16991 CKAP4
HPAiHPA000278
HPA000792
HPA001225
neXtProtiNX_Q07065
OpenTargetsiENSG00000136026
PharmGKBiPA26527
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IIRS Eukaryota
ENOG4111Q54 LUCA
GeneTreeiENSGT00390000015968
HOGENOMiHOG000060146
HOVERGENiHBG101940
InParanoidiQ07065
KOiK13999
OMAiHQDFSRQ
OrthoDBiEOG091G067G
PhylomeDBiQ07065
TreeFamiTF332395

Enzyme and pathway databases

ReactomeiR-HSA-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-HSA-5683826 Surfactant metabolism
R-HSA-6798695 Neutrophil degranulation
R-HSA-8957275 Post-translational protein phosphorylation

Miscellaneous databases

ChiTaRSiCKAP4 human
GeneWikiiCKAP4
GenomeRNAii10970
PROiPR:Q07065

Gene expression databases

BgeeiENSG00000136026 Expressed in 243 organ(s), highest expression level in tibia
CleanExiHS_CKAP4
ExpressionAtlasiQ07065 baseline and differential
GenevisibleiQ07065 HS

Family and domain databases

InterProiView protein in InterPro
IPR028428 Ckap4
PANTHERiPTHR45161 PTHR45161, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCKAP4_HUMAN
AccessioniPrimary (citable) accession number: Q07065
Secondary accession number(s): Q504S5, Q53ES6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: June 1, 2002
Last modified: November 7, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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