UniProtKB - Q06DK7 (TETX_SPHSM)
Flavin-dependent monooxygenase
tet(X)
Functioni
An FAD-requiring monooxygenase active on some tetracycline antibiotic derivatives, which leads to their inactivation. Hydroxylates carbon 11a of tetracycline and some analogs.
UniRule annotationThis bacteria is able to grow in the presence of moderate concentrations of tetracycline and its analogs, presumably due to the presence of this protein (PubMed:19187139).
Expression in E.coli leads to breakdown of tetracycline; the initial products include a monooxygenated compound. After 2 hours intact tetracycline is no longer detectable in the culture supernatant, while degradation intermediates remain detectable for at least 20 hours (PubMed:26038239).
By similarity2 PublicationsMiscellaneous
Catalytic activityi
- a tetracycline + H+ + NADPH + O2 = an 11a-hydroxytetracycline + H2O + NADP+UniRule annotation1 Publication
- EC:1.14.13.2311 Publication
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 54 | NADPHUniRule annotation | 1 | |
Binding sitei | 61 | FADUniRule annotation | 1 | |
Binding sitei | 117 | FADUniRule annotation | 1 | |
Binding sitei | 311 | FADUniRule annotation | 1 |
GO - Molecular functioni
- FAD binding Source: InterPro
- monooxygenase activity Source: UniProtKB-UniRule
GO - Biological processi
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Antibiotic resistance |
Ligand | FAD, Flavoprotein, NADP, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Flavin-dependent monooxygenaseUniRule annotationAlternative name(s): TetX monooxygenaseUniRule annotation (EC:1.14.13.231UniRule annotation1 Publication) Short name: TetXUniRule annotation Tetracycline resistance protein1 Publication |
Gene namesi | Name:tet(X)1 Publication |
Organismi | Sphingobacterium sp. (strain PM2-P1-29) |
Taxonomic identifieri | 403776 [NCBI] |
Taxonomic lineagei | Bacteria › Bacteroidetes › Sphingobacteriia › Sphingobacteriales › Sphingobacteriaceae › Sphingobacterium › |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000448379 | 1 – 388 | Flavin-dependent monooxygenaseAdd BLAST | 388 |
Interactioni
Subunit structurei
Monomer.
UniRule annotationFamily & Domainsi
Domaini
Sequence similaritiesi
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_00845, TetX_monooxygenase, 1 hit |
InterProi | View protein in InterPro IPR002938, FAD-bd IPR036188, FAD/NAD-bd_sf IPR043683, TetX_monooxygenase |
Pfami | View protein in Pfam PF01494, FAD_binding_3, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTMRIDTDKQ MNLLSDKNVA IIGGGPVGLT MAKLLQQNGI DVSVYERDND
60 70 80 90 100
REARIFGGTL DLHKGSGQEA MKKAGLLQTY YDLALPMGVN IADKKGNILS
110 120 130 140 150
TKNVKPENRF DNPEINRNDL RAILLNSLEN DTVIWDRKLV MLEPGKKKWT
160 170 180 190 200
LTFENKPSET ADLVILANGG MSKVRKFVTD TEVEETGTFN IQADIHQPEI
210 220 230 240 250
NCPGFFQLCN GNRLMASHQG NLLFANPNNN GALHFGISFK TPDEWKNQTQ
260 270 280 290 300
VDFQNRNSVV DFLLKEFSDW DERYKELIHT TLSFVGLATR IFPLEKPWKS
310 320 330 340 350
KRPLPITMIG DAAHLMPPFA GQGVNSGLVD ALILSDNLAD GKFNSIEEAV
360 370 380
KNYEQQMFMY GKEAQEESTQ NEIEMFKPDF TFQQLLNV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU864422 Genomic DNA Translation: ABI95380.2 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU864422 Genomic DNA Translation: ABI95380.2 |
3D structure databases
SMRi | Q06DK7 |
ModBasei | Search... |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_00845, TetX_monooxygenase, 1 hit |
InterProi | View protein in InterPro IPR002938, FAD-bd IPR036188, FAD/NAD-bd_sf IPR043683, TetX_monooxygenase |
Pfami | View protein in Pfam PF01494, FAD_binding_3, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TETX_SPHSM | |
Accessioni | Q06DK7Primary (citable) accession number: Q06DK7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 16, 2019 |
Last sequence update: | September 23, 2008 | |
Last modified: | December 2, 2020 | |
This is version 43 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Transposable elementDocuments
- SIMILARITY comments
Index of protein domains and families