UniProtKB - Q06AK7 (TOP1_STAAU)
Protein
DNA topoisomerase 1
Gene
topA
Organism
Staphylococcus aureus
Status
Functioni
Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation
Catalytic activityi
- ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation EC:5.6.2.1
Cofactori
Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 9 | Magnesium 1; catalyticUniRule annotation | 1 | |
Metal bindingi | 82 | Magnesium 1; catalyticUniRule annotation | 1 | |
Metal bindingi | 82 | Magnesium 2UniRule annotation | 1 | |
Metal bindingi | 84 | Magnesium 2UniRule annotation | 1 | |
Active sitei | 298 | O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 577 – 603 | C4-type 1Add BLAST | 27 | |
Zinc fingeri | 617 – 645 | C4-type 2Add BLAST | 29 | |
Zinc fingeri | 658 – 681 | C4-type 3Add BLAST | 24 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA topoisomerase type I (single strand cut, ATP-independent) activity Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- DNA topological change Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-binding, Isomerase, Topoisomerase |
Ligand | Magnesium, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 5.99.1.2, 3352 |
Names & Taxonomyi
Protein namesi | Recommended name: DNA topoisomerase 1UniRule annotation (EC:5.6.2.1UniRule annotation)Alternative name(s): DNA topoisomerase IUniRule annotation Omega-protein Relaxing enzyme Swivelase Untwisting enzyme |
Gene namesi | Name:topAUniRule annotation |
Organismi | Staphylococcus aureus |
Taxonomic identifieri | 1280 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000285934 | 1 – 689 | DNA topoisomerase 1Add BLAST | 689 |
Interactioni
Subunit structurei
Monomer.
UniRule annotationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 33 | Interaction with DNAUniRule annotation | 1 | |
Sitei | 139 | Interaction with DNAUniRule annotation | 1 | |
Sitei | 140 | Interaction with DNAUniRule annotation | 1 | |
Sitei | 143 | Interaction with DNAUniRule annotation | 1 | |
Sitei | 148 | Interaction with DNAUniRule annotation | 1 | |
Sitei | 155 | Interaction with DNAUniRule annotation | 1 | |
Sitei | 300 | Interaction with DNAUniRule annotation | 1 | |
Sitei | 488 | Interaction with DNAUniRule annotation | 1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 113 | ToprimUniRule annotationAdd BLAST | 111 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 163 – 168 | Interaction with DNAUniRule annotation | 6 |
Sequence similaritiesi
Belongs to the type IA topoisomerase family.UniRule annotation
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 577 – 603 | C4-type 1Add BLAST | 27 | |
Zinc fingeri | 617 – 645 | C4-type 2Add BLAST | 29 | |
Zinc fingeri | 658 – 681 | C4-type 3Add BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerFamily and domain databases
CDDi | cd00186, TOP1Ac, 1 hit cd03363, TOPRIM_TopoIA_TopoI, 1 hit |
Gene3Di | 1.10.290.10, 1 hit 1.10.460.10, 1 hit 2.70.20.10, 1 hit |
HAMAPi | MF_00952, Topoisom_1_prok, 1 hit |
InterProi | View protein in InterPro IPR000380, Topo_IA IPR003601, Topo_IA_2 IPR023406, Topo_IA_AS IPR013497, Topo_IA_cen IPR013824, Topo_IA_cen_sub1 IPR013825, Topo_IA_cen_sub2 IPR013826, Topo_IA_cen_sub3 IPR023405, Topo_IA_core_domain IPR003602, Topo_IA_DNA-bd_dom IPR013498, Topo_IA_Znf IPR005733, TopoI_bac-type IPR028612, Topoisom_1_IA IPR006171, TOPRIM_domain IPR034149, TOPRIM_TopoI |
PANTHERi | PTHR42785, PTHR42785, 1 hit |
Pfami | View protein in Pfam PF01131, Topoisom_bac, 1 hit PF01751, Toprim, 1 hit PF01396, zf-C4_Topoisom, 3 hits |
PRINTSi | PR00417, PRTPISMRASEI |
SMARTi | View protein in SMART SM00437, TOP1Ac, 1 hit SM00436, TOP1Bc, 1 hit SM00493, TOPRIM, 1 hit |
SUPFAMi | SSF56712, SSF56712, 1 hit |
TIGRFAMsi | TIGR01051, topA_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00396, TOPOISOMERASE_I_PROK, 1 hit PS50880, TOPRIM, 1 hit |
i Sequence
Sequence statusi: Complete.
Q06AK7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADNLVIVES PAKAKTIEKY LGKKYKVIAS MGHVRDLPRS QMGVDTEDNY
60 70 80 90 100
EPKYITIRGK GPVVKELKKH AKKAKNVFLA SDPDREGEAI AWHLSKILEL
110 120 130 140 150
EDSKENRVVF NEITKDAVKE SFKNPREIEM NLVDAQQARR ILDRLVGYNI
160 170 180 190 200
SPVLWKKVKK GLSAGRVQSV ALRLVIDREN EIRNFKPEEY WTIEGEFRYK
210 220 230 240 250
KSKFNAKFLH YKNKPFKLKT KKDVEKITTA LDGDQFEITN VTKKEKTRNP
260 270 280 290 300
ANPFTTSTLQ QEAARKLNFK ARKTMMVAQQ LYEGIDLKKQ GTIGLITYMR
310 320 330 340 350
TDSTRISDTA KAEAKQYITN KYGESYTSKR KASGKQGDQD AHEAIRPSST
360 370 380 390 400
MRTPDDMKSF LTKDQYRLYK LIWERFVASQ MAPAILDTVS LDITQGDIKF
410 420 430 440 450
RANGQTIKFK GFMTLYVETK DDSDSEKENK LPKLEQGDKV TATQIEPAQH
460 470 480 490 500
YTQPPPRYTE ARLVKTLEEL KIGRPSTYAP TIDTIQKRNY VKLESKRFVP
510 520 530 540 550
TELGEIVHEQ VKEYFPEIID VEFTVNMETL LDKIAEGDIT WRKVIDGFFS
560 570 580 590 600
SFKQDVERAE EEMEKIEIKD EPAGEDCEVC GSPMVIKMGR YGKFMACSNF
610 620 630 640 650
PDCRNTKAIV KSIGVKCPKC NDGDVVERKS KKNRVFYGCS KYPECDFISW
660 670 680
DKPIGRDCPK CNQYLVENKK GKTTQVICSN CDYKEAAQK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ923160 Genomic DNA Translation: ABI81408.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ923160 Genomic DNA Translation: ABI81408.1 |
3D structure databases
SMRi | Q06AK7 |
ModBasei | Search... |
Chemistry databases
DrugBanki | DB01051, Novobiocin DB00487, Pefloxacin |
Enzyme and pathway databases
BRENDAi | 5.99.1.2, 3352 |
Family and domain databases
CDDi | cd00186, TOP1Ac, 1 hit cd03363, TOPRIM_TopoIA_TopoI, 1 hit |
Gene3Di | 1.10.290.10, 1 hit 1.10.460.10, 1 hit 2.70.20.10, 1 hit |
HAMAPi | MF_00952, Topoisom_1_prok, 1 hit |
InterProi | View protein in InterPro IPR000380, Topo_IA IPR003601, Topo_IA_2 IPR023406, Topo_IA_AS IPR013497, Topo_IA_cen IPR013824, Topo_IA_cen_sub1 IPR013825, Topo_IA_cen_sub2 IPR013826, Topo_IA_cen_sub3 IPR023405, Topo_IA_core_domain IPR003602, Topo_IA_DNA-bd_dom IPR013498, Topo_IA_Znf IPR005733, TopoI_bac-type IPR028612, Topoisom_1_IA IPR006171, TOPRIM_domain IPR034149, TOPRIM_TopoI |
PANTHERi | PTHR42785, PTHR42785, 1 hit |
Pfami | View protein in Pfam PF01131, Topoisom_bac, 1 hit PF01751, Toprim, 1 hit PF01396, zf-C4_Topoisom, 3 hits |
PRINTSi | PR00417, PRTPISMRASEI |
SMARTi | View protein in SMART SM00437, TOP1Ac, 1 hit SM00436, TOP1Bc, 1 hit SM00493, TOPRIM, 1 hit |
SUPFAMi | SSF56712, SSF56712, 1 hit |
TIGRFAMsi | TIGR01051, topA_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00396, TOPOISOMERASE_I_PROK, 1 hit PS50880, TOPRIM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TOP1_STAAU | |
Accessioni | Q06AK7Primary (citable) accession number: Q06AK7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | October 31, 2006 | |
Last modified: | December 2, 2020 | |
This is version 77 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |