UniProtKB - Q06902 (PEPA_ASPOR)
Aspergillopepsin-1
pepA
Functioni
Secreted aspartic endopeptidase that allows assimilation of proteinaceous substrates. The scissile peptide bond is attacked by a nucleophilic water molecule activated by two aspartic residues in the active site. Shows a broad primary substrate specificity. Favors hydrophobic residues at the P1 and P1' positions, but also accepts a lysine residue in the P1 position, leading to the activation of trypsinogen and chymotrypsinogen A. Hydrolyzes bovine chymotrysinogen A between positions 'Arg-15' and 'Ile-16'.
1 PublicationCatalytic activityi
- Hydrolysis of proteins with broad specificity. Generally favors hydrophobic residues in P1 and P1', but also accepts Lys in P1, which leads to activation of trypsinogen. Does not clot milk.1 Publication EC:3.4.23.18
Activity regulationi
Kineticsi
- KM=0.064 mM for Z-His-Phe-Phe-OEt1 Publication
- KM=0.037 mM for Z-Ala-Ala-Phe-Phe-OPy4Pr1 Publication
- KM=0.1 mM for bovine trypsinogen1 Publication
- KM=0.18 mM for bovine chymotrysinogen A1 Publication
pH dependencei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 111 | PROSITE-ProRule annotation | 1 | |
Active sitei | 293 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- aspartic-type endopeptidase activity Source: UniProtKB-KW
Keywordsi
Molecular function | Aspartyl protease, Hydrolase, Protease |
Enzyme and pathway databases
BRENDAi | 3.4.23.18, 522 |
Protein family/group databases
MEROPSi | A01.026 |
Names & Taxonomyi
Protein namesi | Recommended name: Aspergillopepsin-1Curated (EC:3.4.23.181 Publication)Alternative name(s): Acid protease A21 Publication Aspartic protease pepA Aspergillopepsin I Aspergillopepsin O1 Publication Short name: PEPO Aspergillopeptidase A Short name: PEPA |
Gene namesi | Name:pepA1 Publication Synonyms:pepO1 Publication ORF Names:AO090120000474 |
Organismi | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
Taxonomic identifieri | 510516 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Circumdati › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:AO090120000474 |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: AspGD
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
PropeptideiPRO_0000025922 | 21 – 77 | Activation peptideBy similarityAdd BLAST | 57 | |
ChainiPRO_0000025923 | 78 – 404 | Aspergillopepsin-1Add BLAST | 327 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 140 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 329 ↔ 364 | PROSITE-ProRule annotation |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 95 – 401 | Peptidase A1PROSITE-ProRule annotationAdd BLAST | 307 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
HOGENOMi | CLU_013253_0_1_1 |
OMAi | PSSHCRF |
Family and domain databases
CDDi | cd06097, Aspergillopepsin_like, 1 hit |
Gene3Di | 2.40.70.10, 2 hits |
InterProi | View protein in InterPro IPR001461, Aspartic_peptidase_A1 IPR001969, Aspartic_peptidase_AS IPR034163, Aspergillopepsin-like_cat_dom IPR033121, PEPTIDASE_A1 IPR021109, Peptidase_aspartic_dom_sf |
PANTHERi | PTHR47966, PTHR47966, 1 hit |
Pfami | View protein in Pfam PF00026, Asp, 1 hit |
PRINTSi | PR00792, PEPSIN |
SUPFAMi | SSF50630, SSF50630, 1 hit |
PROSITEi | View protein in PROSITE PS00141, ASP_PROTEASE, 2 hits PS51767, PEPTIDASE_A1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MVILSKVAAV AVGLSTVASA LPTGPSHSPH ARRGFTINQI TRQTARVGPK
60 70 80 90 100
TASFPAIYSR ALAKYGGTVP AHLKSAVASG HGTVVTSPEP NDIEYLTPVN
110 120 130 140 150
IGGTTLNLDF DTGSADLWVF SEELPKSEQT GHDVYKPSGN ASKIAGASWD
160 170 180 190 200
ISYGDGSSAS GDVYQDTVTV GGVTAQGQAV EAASKISDQF VQDKNNDGLL
210 220 230 240 250
GLAFSSINTV KPKPQTTFFD TVKDQLDAPL FAVTLKYHAP GSYDFGFIDK
260 270 280 290 300
SKFTGELAYA DVDDSQGFWQ FTADGYSVGK GDAQKAPITG IADTGTTLVM
310 320 330 340 350
LDDEIVDAYY KQVQGAKNDA SAGGYVFPCE TELPEFTVVI GSYNAVIPGK
360 370 380 390 400
HINYAPLQEG SSTCVGGIQS NSGLGLSILG DVFLKSQYVV FDSQGPRLGF
AAQA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 289 | T → S in BAA02994 (PubMed:7763981).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13894 Genomic DNA Translation: BAA02994.1 M92927 Genomic DNA Translation: AAA32704.1 AP007166 Genomic DNA Translation: BAE63042.1 |
PIRi | JN0630 |
RefSeqi | XP_001824175.1, XM_001824123.2 |
Genome annotation databases
EnsemblFungii | BAE63042; BAE63042; AO090120000474 |
GeneIDi | 5996434 |
KEGGi | aor:AO090120000474 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13894 Genomic DNA Translation: BAA02994.1 M92927 Genomic DNA Translation: AAA32704.1 AP007166 Genomic DNA Translation: BAE63042.1 |
PIRi | JN0630 |
RefSeqi | XP_001824175.1, XM_001824123.2 |
3D structure databases
SMRi | Q06902 |
ModBasei | Search... |
Protein family/group databases
MEROPSi | A01.026 |
Genome annotation databases
EnsemblFungii | BAE63042; BAE63042; AO090120000474 |
GeneIDi | 5996434 |
KEGGi | aor:AO090120000474 |
Organism-specific databases
VEuPathDBi | FungiDB:AO090120000474 |
Phylogenomic databases
HOGENOMi | CLU_013253_0_1_1 |
OMAi | PSSHCRF |
Enzyme and pathway databases
BRENDAi | 3.4.23.18, 522 |
Family and domain databases
CDDi | cd06097, Aspergillopepsin_like, 1 hit |
Gene3Di | 2.40.70.10, 2 hits |
InterProi | View protein in InterPro IPR001461, Aspartic_peptidase_A1 IPR001969, Aspartic_peptidase_AS IPR034163, Aspergillopepsin-like_cat_dom IPR033121, PEPTIDASE_A1 IPR021109, Peptidase_aspartic_dom_sf |
PANTHERi | PTHR47966, PTHR47966, 1 hit |
Pfami | View protein in Pfam PF00026, Asp, 1 hit |
PRINTSi | PR00792, PEPSIN |
SUPFAMi | SSF50630, SSF50630, 1 hit |
PROSITEi | View protein in PROSITE PS00141, ASP_PROTEASE, 2 hits PS51767, PEPTIDASE_A1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PEPA_ASPOR | |
Accessioni | Q06902Primary (citable) accession number: Q06902 Secondary accession number(s): Q00249, Q2U5X3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | May 2, 2006 | |
Last modified: | September 29, 2021 | |
This is version 137 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families