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UniProtKB - Q06893 (VANB_ENTFA)
Protein
Vancomycin B-type resistance protein VanB
Gene
vanB
Organism
Enterococcus faecalis (strain ATCC 700802 / V583)
Status
Functioni
Required for high-level resistance to glycopeptides antibiotics. D-Ala--D-Ala ligase of altered specificity which catalyzes ester bond formation between D-Ala and various D-hydroxy acids; producing a peptidoglycan which does not terminate in D-alanine but in D-lactate, thus preventing vancomycin binding.
Catalytic activityi
- EC:6.1.2.11 Publication
Cofactori
Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 132 | ATPBy similarity | 1 | |
Binding sitei | 240 | ATP; via amide nitrogenBy similarity | 1 | |
Binding sitei | 243 | SubstrateBy similarity | 1 | |
Metal bindingi | 304 | Magnesium 1By similarity | 1 | |
Metal bindingi | 304 | Magnesium 2By similarity | 1 | |
Metal bindingi | 306 | Magnesium 2By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 168 – 170 | ATPBy similarity | 3 | |
Nucleotide bindingi | 176 – 177 | ATPBy similarity | 2 | |
Nucleotide bindingi | 206 – 213 | ATPBy similarity | 8 | |
Nucleotide bindingi | 303 – 304 | ATPBy similarity | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- D-alanine-D-alanine ligase activity Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: UniProtKB-UniRule
- regulation of cell shape Source: UniProtKB-KW
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EFAE226185:G1G0C-2250-MONOMER |
SABIO-RKi | Q06893 |
Names & Taxonomyi
Protein namesi | Recommended name: Vancomycin B-type resistance protein VanB (EC:6.1.2.1)Alternative name(s): D-alanine--D-lactate ligase VanB ligase |
Gene namesi | Name:vanB Synonyms:vanB2 Ordered Locus Names:EF_2294 |
Organismi | Enterococcus faecalis (strain ATCC 700802 / V583) |
Taxonomic identifieri | 226185 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Lactobacillales › Enterococcaceae › Enterococcus › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-UniRule
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000177918 | 1 – 342 | Vancomycin B-type resistance protein VanBAdd BLAST | 342 |
Expressioni
Inductioni
By vancomycin.
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 136 – 337 | ATP-graspAdd BLAST | 202 |
Sequence similaritiesi
Belongs to the D-alanine--D-alanine ligase family.Curated
Phylogenomic databases
eggNOGi | COG1181, Bacteria |
HOGENOMi | CLU_039268_0_0_9 |
OMAi | IPYVGCD |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
HAMAPi | MF_00047, Dala_Dala_lig, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR000291, D-Ala_lig_Van_CS IPR005905, D_ala_D_ala IPR011095, Dala_Dala_lig_C IPR011127, Dala_Dala_lig_N IPR016185, PreATP-grasp_dom_sf |
Pfami | View protein in Pfam PF07478, Dala_Dala_lig_C, 1 hit PF01820, Dala_Dala_lig_N, 1 hit |
PIRSFi | PIRSF039102, Ddl/VanB, 1 hit |
SUPFAMi | SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01205, D_ala_D_alaTIGR, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS00843, DALA_DALA_LIGASE_1, 1 hit PS00844, DALA_DALA_LIGASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q06893-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNKIKVAIIF GGCSEEHDVS VKSAIEIAAN INTEKFDPHY IGITKNGVWK
60 70 80 90 100
LCKKPCTEWE ADSLPAIFSP DRKTHGLLVM KEREYETRRI DVAFPVLHGK
110 120 130 140 150
CGEDGAIQGL FELSGIPYVG CDIQSSAACM DKSLAYILTK NAGIAVPEFQ
160 170 180 190 200
MIEKGDKPEA RTLTYPVFVK PARSGSSFGV TKVNSTEELN AAIEAAGQYD
210 220 230 240 250
GKILIEQAIS GCEVGCAVMG NEDDLIVGEV DQIRLSHGIF RIHQENEPEK
260 270 280 290 300
GSENAMIIVP ADIPVEERNR VQETAKKVYR VLGCRGLARV DLFLQEDGGI
310 320 330 340
VLNEVNTLPG FTSYSRYPRM AAAAGITLPA LIDSLITLAI ER
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 106 | A → C in strain: SF300. | 1 | |
Natural varianti | 153 | E → D in strain: SF300. | 1 | |
Natural varianti | 161 – 162 | RT → GA in strain: SF300. | 2 | |
Natural varianti | 185 | S → G in strain: SF300. | 1 | |
Natural varianti | 258 | I → T in strain: SF300. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00456 Unassigned DNA Translation: AAC43217.1 U35369 Genomic DNA Translation: AAB05627.1 AE016830 Genomic DNA Translation: AAO82021.1 L06138 Genomic DNA No translation available. L15304 Genomic DNA Translation: AAA24785.1 |
PIRi | PN0478 |
RefSeqi | NP_815951.1, NC_004668.1 WP_002368691.1, NZ_KE136528.1 |
Genome annotation databases
EnsemblBacteriai | AAO82021; AAO82021; EF_2294 |
KEGGi | efa:EF2294 |
PATRICi | fig|226185.45.peg.1238 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00456 Unassigned DNA Translation: AAC43217.1 U35369 Genomic DNA Translation: AAB05627.1 AE016830 Genomic DNA Translation: AAO82021.1 L06138 Genomic DNA No translation available. L15304 Genomic DNA Translation: AAA24785.1 |
PIRi | PN0478 |
RefSeqi | NP_815951.1, NC_004668.1 WP_002368691.1, NZ_KE136528.1 |
3D structure databases
SMRi | Q06893 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 226185.EF_2294 |
Genome annotation databases
EnsemblBacteriai | AAO82021; AAO82021; EF_2294 |
KEGGi | efa:EF2294 |
PATRICi | fig|226185.45.peg.1238 |
Phylogenomic databases
eggNOGi | COG1181, Bacteria |
HOGENOMi | CLU_039268_0_0_9 |
OMAi | IPYVGCD |
Enzyme and pathway databases
BioCyci | EFAE226185:G1G0C-2250-MONOMER |
SABIO-RKi | Q06893 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
HAMAPi | MF_00047, Dala_Dala_lig, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR000291, D-Ala_lig_Van_CS IPR005905, D_ala_D_ala IPR011095, Dala_Dala_lig_C IPR011127, Dala_Dala_lig_N IPR016185, PreATP-grasp_dom_sf |
Pfami | View protein in Pfam PF07478, Dala_Dala_lig_C, 1 hit PF01820, Dala_Dala_lig_N, 1 hit |
PIRSFi | PIRSF039102, Ddl/VanB, 1 hit |
SUPFAMi | SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01205, D_ala_D_alaTIGR, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS00843, DALA_DALA_LIGASE_1, 1 hit PS00844, DALA_DALA_LIGASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VANB_ENTFA | |
Accessioni | Q06893Primary (citable) accession number: Q06893 Secondary accession number(s): Q47821, Q57112 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families