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UniProtKB - Q06892 (POS5_YEAST)
Protein
NADH kinase POS5, mitochondrial
Gene
POS5
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Phosphorylates both NADH and NAD+, with a twofold preference for NADH. Anti-oxidant factor and key source of the cellular reductant NADPH.
2 PublicationsMiscellaneous
Present with 4650 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Kineticsi
- KM=105 µM for NADH1 Publication
- KM=2.1 mM for ATP1 Publication
pH dependencei
Optimum pH is 8.5.1 Publication
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- NAD+ kinase activity Source: GO_Central
- NADH kinase activity Source: SGD
GO - Biological processi
- cellular response to oxidative stress Source: SGD
- NAD metabolic process Source: InterPro
- NADP biosynthetic process Source: SGD
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, NAD, NADP, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.1.23, 984 2.7.1.86, 984 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:POS5 Ordered Locus Names:YPL188W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006109, POS5 |
VEuPathDBi | FungiDB:YPL188W |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix 4 Publications
Mitochondrion
- mitochondrial matrix Source: SGD
- mitochondrion Source: SGD
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | MitochondrionSequence analysis | ||
ChainiPRO_0000120715 | ? – 414 | NADH kinase POS5, mitochondrial |
Proteomic databases
MaxQBi | Q06892 |
PaxDbi | Q06892 |
PRIDEi | Q06892 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 35995, 58 interactors |
DIPi | DIP-5503N |
IntActi | Q06892, 3 interactors |
STRINGi | 4932.YPL188W |
Miscellaneous databases
RNActi | Q06892, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q06892 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the NAD kinase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2178, Eukaryota |
HOGENOMi | CLU_008831_10_2_1 |
InParanoidi | Q06892 |
OMAi | PFHIDDF |
Family and domain databases
Gene3Di | 2.60.200.30, 1 hit 3.40.50.10330, 1 hit |
HAMAPi | MF_00361, NAD_kinase, 1 hit |
InterProi | View protein in InterPro IPR017438, ATP-NAD_kinase_N IPR017437, ATP-NAD_kinase_PpnK-typ_C IPR016064, NAD/diacylglycerol_kinase_sf IPR002504, NADK |
Pfami | View protein in Pfam PF01513, NAD_kinase, 1 hit |
SUPFAMi | SSF111331, SSF111331, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q06892-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFVRVKLNKP VKWYRFYSTL DSHSLKLQSG SKFVKIKPVN NLRSSSSADF
60 70 80 90 100
VSPPNSKLQS LIWQNPLQNV YITKKPWTPS TREAMVEFIT HLHESYPEVN
110 120 130 140 150
VIVQPDVAEE ISQDFKSPLE NDPNRPHILY TGPEQDIVNR TDLLVTLGGD
160 170 180 190 200
GTILHGVSMF GNTQVPPVLA FALGTLGFLS PFDFKEHKKV FQEVISSRAK
210 220 230 240 250
CLHRTRLECH LKKKDSNSSI VTHAMNDIFL HRGNSPHLTN LDIFIDGEFL
260 270 280 290 300
TRTTADGVAL ATPTGSTAYS LSAGGSIVSP LVPAILMTPI CPRSLSFRPL
310 320 330 340 350
ILPHSSHIRI KIGSKLNQKP VNSVVKLSVD GIPQQDLDVG DEIYVINEVG
360 370 380 390 400
TIYIDGTQLP TTRKTENDFN NSKKPKRSGI YCVAKTENDW IRGINELLGF
410
NSSFRLTKRQ TDND
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 37 – 38 | KP → EA in CAA59017 (Ref. 1) Curated | 2 | |
Sequence conflicti | 180 | S → L in CAA59017 (Ref. 1) Curated | 1 | |
Sequence conflicti | 329 | V → D in CAA59017 (Ref. 1) Curated | 1 | |
Sequence conflicti | 343 | I → S in CAA59017 (Ref. 1) Curated | 1 | |
Sequence conflicti | 398 – 401 | LGFN → CRIH in CAA59017 (Ref. 1) Curated | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X84260 Genomic DNA Translation: CAA59017.1 Z73544 Genomic DNA Translation: CAA97900.1 BK006949 Genomic DNA Translation: DAA11247.1 |
PIRi | S65200 |
RefSeqi | NP_015136.1, NM_001184002.1 |
Genome annotation databases
EnsemblFungii | YPL188W_mRNA; YPL188W; YPL188W |
GeneIDi | 855913 |
KEGGi | sce:YPL188W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X84260 Genomic DNA Translation: CAA59017.1 Z73544 Genomic DNA Translation: CAA97900.1 BK006949 Genomic DNA Translation: DAA11247.1 |
PIRi | S65200 |
RefSeqi | NP_015136.1, NM_001184002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3AFO | X-ray | 2.00 | A/B | 27-414 | [»] | |
SMRi | Q06892 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35995, 58 interactors |
DIPi | DIP-5503N |
IntActi | Q06892, 3 interactors |
STRINGi | 4932.YPL188W |
Proteomic databases
MaxQBi | Q06892 |
PaxDbi | Q06892 |
PRIDEi | Q06892 |
Genome annotation databases
EnsemblFungii | YPL188W_mRNA; YPL188W; YPL188W |
GeneIDi | 855913 |
KEGGi | sce:YPL188W |
Organism-specific databases
SGDi | S000006109, POS5 |
VEuPathDBi | FungiDB:YPL188W |
Phylogenomic databases
eggNOGi | KOG2178, Eukaryota |
HOGENOMi | CLU_008831_10_2_1 |
InParanoidi | Q06892 |
OMAi | PFHIDDF |
Enzyme and pathway databases
BRENDAi | 2.7.1.23, 984 2.7.1.86, 984 |
Miscellaneous databases
PROi | PR:Q06892 |
RNActi | Q06892, protein |
Family and domain databases
Gene3Di | 2.60.200.30, 1 hit 3.40.50.10330, 1 hit |
HAMAPi | MF_00361, NAD_kinase, 1 hit |
InterProi | View protein in InterPro IPR017438, ATP-NAD_kinase_N IPR017437, ATP-NAD_kinase_PpnK-typ_C IPR016064, NAD/diacylglycerol_kinase_sf IPR002504, NADK |
Pfami | View protein in Pfam PF01513, NAD_kinase, 1 hit |
SUPFAMi | SSF111331, SSF111331, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | POS5_YEAST | |
Accessioni | Q06892Primary (citable) accession number: Q06892 Secondary accession number(s): D6W3I1, Q08928 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | December 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families