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UniProtKB - Q06833 (NVJ2_YEAST)
Protein
Nucleus-vacuole junction protein 2
Gene
NVJ2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
During endoplasmic reticulum (ER) stress or when cellular ceramide levels increase, induces contacts between the ER and medial-Golgi complex to facilitate non-vesicular transport of ceramides from the ER to the Golgi complex where they are converted to complex sphingolipids, preventing toxic ceramide accumulation.
1 PublicationMiscellaneous
Present with 36500 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- lipid binding Source: SGD
GO - Biological processi
- ER to Golgi ceramide transport Source: UniProtKB
Keywordsi
Biological process | Lipid transport, Transport |
Ligand | Lipid-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Nucleus-vacuole junction protein 21 Publication |
Gene namesi | Ordered Locus Names:YPR091C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006295, NVJ2 |
VEuPathDBi | FungiDB:YPR091C |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Single-pass type II membrane protein Curated
Nucleus
- Nucleus membrane 1 Publication; Single-pass type II membrane protein Curated
Note: Enriched at the nucleus-vacuole junction where it becomes increasingly concentrated as cells enter into the late-logarithmic growth phase (PubMed:22250200). During endoplasmic reticulum (ER) stress, localizes to ER-Golgi contacts (PubMed:28011845).2 Publications
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Nucleus
- nuclear membrane Source: UniProtKB-SubCell
Other locations
- cell periphery Source: SGD
- integral component of membrane Source: UniProtKB-KW
- nucleus-vacuole junction Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 5 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 6 – 26 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 27 – 770 | LumenalSequence analysisAdd BLAST | 744 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 340 | L → A: Failure to mediate ceramide transport; when associated with A-472. 1 Publication | 1 | |
Mutagenesisi | 472 | I → A: Failure to mediate ceramide transport; when associated with A-340. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000257815 | 1 – 770 | Nucleus-vacuole junction protein 2Add BLAST | 770 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 228 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 263 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 279 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 300 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 391 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 528 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 529 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 595 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 620 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 640 | PhosphoserineCombined sources | 1 | |
Modified residuei | 669 | PhosphoserineCombined sources | 1 | |
Glycosylationi | 700 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 717 | PhosphoserineCombined sources | 1 | |
Glycosylationi | 718 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 720 | PhosphoserineCombined sources | 1 | |
Modified residuei | 723 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
MaxQBi | Q06833 |
PaxDbi | Q06833 |
PRIDEi | Q06833 |
PTM databases
iPTMneti | Q06833 |
Interactioni
Binary interactionsi
Protein-protein interaction databases
BioGRIDi | 36260, 80 interactors |
IntActi | Q06833, 6 interactors |
MINTi | Q06833 |
STRINGi | 4932.YPR091C |
Miscellaneous databases
RNActi | Q06833, protein |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 114 – 266 | PHAdd BLAST | 153 | |
Domaini | 304 – 504 | SMP-LTDPROSITE-ProRule annotationAdd BLAST | 201 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 541 – 566 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 578 – 600 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 615 – 770 | DisorderedSequence analysisAdd BLAST | 156 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 548 – 566 | Basic and acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 629 – 646 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 691 – 728 | Polar residuesSequence analysisAdd BLAST | 38 | |
Compositional biasi | 739 – 754 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 756 – 770 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Domaini
The SMP-LTD domain is a barrel-like domain that can bind various types of glycerophospholipids in its interior and mediate their transfer between two adjacent bilayers.PROSITE-ProRule annotation1 Publication
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2238, Eukaryota |
GeneTreei | ENSGT00940000173509 |
HOGENOMi | CLU_012852_2_0_1 |
InParanoidi | Q06833 |
OMAi | MDDIVFY |
Family and domain databases
InterProi | View protein in InterPro IPR019411, MMM1_dom IPR001849, PH_domain IPR031468, SMP_LBD |
Pfami | View protein in Pfam PF10296, MMM1, 1 hit PF00169, PH, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit |
PROSITEi | View protein in PROSITE PS51847, SMP, 1 hit |
i Sequence
Sequence statusi: Complete.
Q06833-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASLKVFLAV YLLGGITFLP LVLFTLYKIH LLYSNLKSAS KKELDHDTAD
60 70 80 90 100
EIDEKTRLLA RDIDPEFKAR KLEEQLGVKV FNKGWITVTK QYYYHSSEVA
110 120 130 140 150
VILKNSNNNK DSDTALQEQI LQRTDLKKKQ RFFAVLRHGN LFLYKDDSQN
160 170 180 190 200
ANLVHAISLQ NRFITIWPRF DELGKEELPD ASLFTKRTCI AIFKNDLVSI
210 220 230 240 250
DSKNHNVILP HFDPLTSAES NNGDISTNDT THEYQSQFHS SNQFFLYFDN
260 270 280 290 300
NMDKEDWYYQ LINASKNSNS LSTGLLDPNV SANAAHLKTK DMLQLIQDIN
310 320 330 340 350
STENQLTTKW LNALLGRLFL SLQQTDTLNK FIHEKICKKL NKIKTPGFLD
360 370 380 390 400
DLVVEKVDVG DSAPLFTSPE LLELSPEGST KIAIDVQYRG NLTIIIATKA
410 420 430 440 450
SINLGSRFKQ REVSLQLSIK IKEFSGPLLF LIKPPPSNRI WYAFRTEPIM
460 470 480 490 500
DFEIEPIVSS SKLSYNVVTN AIKSKFAEAV KESLVVPFMD DIVFYPTPNE
510 520 530 540 550
VYRGGIWEEQ DPEAAARART AAAASDMNNT SAKEHLEALQ EGGMKTQSRI
560 570 580 590 600
KKALRPERKK ENLKDLVDAS GVATKTTTQT TVTTATNDDV SSSENSTKSR
610 620 630 640 650
KYFKNSIKKI GRWYKDNVGN SSDTEDMDEI DVQDKKNDDS ADERESDNPI
660 670 680 690 700
LTSNPKMISN RRPVPRRPSQ PLNTLSPKLE GRKEKDTENF PVPPSASNMN
710 720 730 740 750
ASKMFANKEN RKFSVSSNDS QNSLKNGDPH VKASKLESSQ AFVKKTSQNR
760 770
FNDGFFKQDL EFEEQREPKL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U51033 Genomic DNA Translation: AAB68138.1 BK006949 Genomic DNA Translation: DAA11507.1 |
PIRi | S69075 |
RefSeqi | NP_015416.1, NM_001184188.1 |
Genome annotation databases
EnsemblFungii | YPR091C_mRNA; YPR091C; YPR091C |
GeneIDi | 856207 |
KEGGi | sce:YPR091C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U51033 Genomic DNA Translation: AAB68138.1 BK006949 Genomic DNA Translation: DAA11507.1 |
PIRi | S69075 |
RefSeqi | NP_015416.1, NM_001184188.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 36260, 80 interactors |
IntActi | Q06833, 6 interactors |
MINTi | Q06833 |
STRINGi | 4932.YPR091C |
PTM databases
iPTMneti | Q06833 |
Proteomic databases
MaxQBi | Q06833 |
PaxDbi | Q06833 |
PRIDEi | Q06833 |
Genome annotation databases
EnsemblFungii | YPR091C_mRNA; YPR091C; YPR091C |
GeneIDi | 856207 |
KEGGi | sce:YPR091C |
Organism-specific databases
SGDi | S000006295, NVJ2 |
VEuPathDBi | FungiDB:YPR091C |
Phylogenomic databases
eggNOGi | KOG2238, Eukaryota |
GeneTreei | ENSGT00940000173509 |
HOGENOMi | CLU_012852_2_0_1 |
InParanoidi | Q06833 |
OMAi | MDDIVFY |
Miscellaneous databases
PROi | PR:Q06833 |
RNActi | Q06833, protein |
Family and domain databases
InterProi | View protein in InterPro IPR019411, MMM1_dom IPR001849, PH_domain IPR031468, SMP_LBD |
Pfami | View protein in Pfam PF10296, MMM1, 1 hit PF00169, PH, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit |
PROSITEi | View protein in PROSITE PS51847, SMP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NVJ2_YEAST | |
Accessioni | Q06833Primary (citable) accession number: Q06833 Secondary accession number(s): D6W491 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names