UniProtKB - Q06830 (PRDX1_HUMAN)
Protein
Peroxiredoxin-1
Gene
PRDX1
Organism
Homo sapiens (Human)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 (PubMed:9497357). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).By similarity1 Publication
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.1 Publication
Catalytic activityi
- EC:1.11.1.242 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 52 | Cysteine sulfenic acid (-SOH) intermediate2 Publications | 1 |
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
- peroxidase activity Source: MGI
- RNA binding Source: UniProtKB
- thioredoxin peroxidase activity Source: BHF-UCL
GO - Biological processi
- cell population proliferation Source: ProtInc
- cell redox homeostasis Source: GO_Central
- cellular response to oxidative stress Source: Reactome
- hydrogen peroxide catabolic process Source: BHF-UCL
- leukocyte activation Source: GO_Central
- natural killer cell activation Source: UniProtKB
- removal of superoxide radicals Source: GO_Central
- response to oxidative stress Source: GO_Central
- retina homeostasis Source: UniProtKB
- skeletal system development Source: ProtInc
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Enzyme and pathway databases
BioCyci | MetaCyc:HS04134-MONOMER |
BRENDAi | 1.11.1.15, 2681 |
PathwayCommonsi | Q06830 |
Reactomei | R-HSA-3299685, Detoxification of Reactive Oxygen Species R-HSA-5628897, TP53 Regulates Metabolic Genes R-HSA-8862803, Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models |
SIGNORi | Q06830 |
Protein family/group databases
PeroxiBasei | 4501, Hs2CysPrx01 |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxiredoxin-1 (EC:1.11.1.242 Publications)Alternative name(s): Natural killer cell-enhancing factor A Short name: NKEF-A Proliferation-associated gene protein Short name: PAG Thioredoxin peroxidase 2 Thioredoxin-dependent peroxide reductase 2 Thioredoxin-dependent peroxiredoxin 1Curated |
Gene namesi | Name:PRDX1 Synonyms:PAGA, PAGB, TDPX2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9352, PRDX1 |
MIMi | 176763, gene |
neXtProti | NX_Q06830 |
VEuPathDBi | HostDB:ENSG00000117450.13 |
Subcellular locationi
Other locations
- Cytoplasm 2 Publications
- Melanosome 1 Publication
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Cytosol
- cytosol Source: GO_Central
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular space Source: UniProtKB
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- melanosome Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 90 | T → A: Abolishes phosphorylation by CDK1; 30% reduction in enzymatic activity. 1 Publication | 1 | |
Mutagenesisi | 90 | T → D: 87% reduction in enzymatic activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5052 |
MalaCardsi | PRDX1 |
OpenTargetsi | ENSG00000117450 |
PharmGKBi | PA33722 |
Miscellaneous databases
Pharosi | Q06830, Tbio |
Chemistry databases
ChEMBLi | CHEMBL5315 |
DrugBanki | DB11638, Artenimol DB09130, Copper DB09221, Polaprezinc DB01593, Zinc DB14487, Zinc acetate DB14533, Zinc chloride DB14548, Zinc sulfate, unspecified form |
Genetic variation databases
BioMutai | PRDX1 |
DMDMi | 548453 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000135076 | 2 – 199 | Peroxiredoxin-1Add BLAST | 198 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 7 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 7 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources | ||
Modified residuei | 16 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 27 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 32 | PhosphoserineCombined sources | 1 | |
Modified residuei | 35 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 35 | N6-succinyllysine; alternateBy similarity | 1 | |
Disulfide bondi | 52 | Interchain (with C-173); in linked formCombined sources1 Publication | ||
Modified residuei | 90 | Phosphothreonine; by CDK11 Publication | 1 | |
Cross-linki | 120 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 136 | N6-acetyllysineBy similarity | 1 | |
Disulfide bondi | 173 | Interchain (with C-52); in linked formCombined sources1 Publication | ||
Cross-linki | 185 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources |
Post-translational modificationi
Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.1 Publication
The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.6 Publications
Keywords - PTMi
Acetylation, Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q06830 |
jPOSTi | Q06830 |
MassIVEi | Q06830 |
MaxQBi | Q06830 |
PaxDbi | Q06830 |
PeptideAtlasi | Q06830 |
PRIDEi | Q06830 |
ProteomicsDBi | 12697 58486 |
TopDownProteomicsi | Q06830 |
2D gel databases
DOSAC-COBS-2DPAGEi | Q06830 |
OGPi | Q06830 |
SWISS-2DPAGEi | Q06830 |
UCD-2DPAGEi | Q06830 |
PTM databases
iPTMneti | Q06830 |
MetOSitei | Q06830 |
PhosphoSitePlusi | Q06830 |
SwissPalmi | Q06830 |
Expressioni
Inductioni
Constitutively expressed in most human cells; is induced to higher levels upon serum stimulation in untransformed and transformed cells.
Gene expression databases
Bgeei | ENSG00000117450, Expressed in thyroid gland and 241 other tissues |
ExpressionAtlasi | Q06830, baseline and differential |
Genevisiblei | Q06830, HS |
Organism-specific databases
HPAi | ENSG00000117450, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer (PubMed:18172504).
Interacts with GDPD5; forms a mixed-disulfide with GDPD5 (By similarity).
Interacts with SESN1 and SESN2 (PubMed:15105503).
Interacts with FAM107A (PubMed:21969592).
By similarity3 PublicationsBinary interactionsi
Hide detailsQ06830
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 111089, 218 interactors |
CORUMi | Q06830 |
DIPi | DIP-33152N |
IntActi | Q06830, 100 interactors |
MINTi | Q06830 |
STRINGi | 9606.ENSP00000262746 |
Miscellaneous databases
RNActi | Q06830, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q06830 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q06830 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 165 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 160 |
Sequence similaritiesi
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | KOG0852, Eukaryota |
GeneTreei | ENSGT00940000154277 |
InParanoidi | Q06830 |
OMAi | FWYPKDF |
OrthoDBi | 1326484at2759 |
PhylomeDBi | Q06830 |
TreeFami | TF105181 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q06830-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSGNAKIGH PAPNFKATAV MPDGQFKDIS LSDYKGKYVV FFFYPLDFTF
60 70 80 90 100
VCPTEIIAFS DRAEEFKKLN CQVIGASVDS HFCHLAWVNT PKKQGGLGPM
110 120 130 140 150
NIPLVSDPKR TIAQDYGVLK ADEGISFRGL FIIDDKGILR QITVNDLPVG
160 170 180 190
RSVDETLRLV QAFQFTDKHG EVCPAGWKPG SDTIKPDVQK SKEYFSKQK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MSI0 | A0A0A0MSI0_HUMAN | Peroxiredoxin-1 | PRDX1 | 171 | Annotation score: | ||
A0A0A0MRQ5 | A0A0A0MRQ5_HUMAN | Peroxiredoxin-1 | PRDX1 | 97 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 147 | L → P (PubMed:8026862).Curated | 1 | |
Sequence conflicti | 149 – 150 | VG → CC (PubMed:8026862).Curated | 2 | |
Sequence conflicti | 189 | Q → P (PubMed:8026862).Curated | 1 | |
Sequence conflicti | 191 | S → T (PubMed:8026862).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_025050 | 62 | R → G1 PublicationCorresponds to variant dbSNP:rs34034070Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67951 mRNA Translation: CAA48137.1 L19184 mRNA Translation: AAA50464.1 BT019740 mRNA Translation: AAV38545.1 CR407652 mRNA Translation: CAG28580.1 DQ297142 Genomic DNA Translation: ABB84465.1 AB451262 mRNA Translation: BAG70076.1 AB451388 mRNA Translation: BAG70202.1 AL451136 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06975.1 CH471059 Genomic DNA Translation: EAX06976.1 CH471059 Genomic DNA Translation: EAX06978.1 CH471059 Genomic DNA Translation: EAX06979.1 CH471059 Genomic DNA Translation: EAX06980.1 CH471059 Genomic DNA Translation: EAX06981.1 CH471059 Genomic DNA Translation: EAX06982.1 BC007063 mRNA Translation: AAH07063.1 BC021683 mRNA Translation: AAH21683.1 |
CCDSi | CCDS522.1 |
PIRi | A46711 |
RefSeqi | NP_001189360.1, NM_001202431.1 NP_002565.1, NM_002574.3 NP_859047.1, NM_181696.2 NP_859048.1, NM_181697.2 |
Genome annotation databases
Ensembli | ENST00000262746; ENSP00000262746; ENSG00000117450 ENST00000319248; ENSP00000361152; ENSG00000117450 |
GeneIDi | 5052 |
KEGGi | hsa:5052 |
UCSCi | uc001coa.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67951 mRNA Translation: CAA48137.1 L19184 mRNA Translation: AAA50464.1 BT019740 mRNA Translation: AAV38545.1 CR407652 mRNA Translation: CAG28580.1 DQ297142 Genomic DNA Translation: ABB84465.1 AB451262 mRNA Translation: BAG70076.1 AB451388 mRNA Translation: BAG70202.1 AL451136 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06975.1 CH471059 Genomic DNA Translation: EAX06976.1 CH471059 Genomic DNA Translation: EAX06978.1 CH471059 Genomic DNA Translation: EAX06979.1 CH471059 Genomic DNA Translation: EAX06980.1 CH471059 Genomic DNA Translation: EAX06981.1 CH471059 Genomic DNA Translation: EAX06982.1 BC007063 mRNA Translation: AAH07063.1 BC021683 mRNA Translation: AAH21683.1 |
CCDSi | CCDS522.1 |
PIRi | A46711 |
RefSeqi | NP_001189360.1, NM_001202431.1 NP_002565.1, NM_002574.3 NP_859047.1, NM_181696.2 NP_859048.1, NM_181697.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2RII | X-ray | 2.60 | A/B | 1-199 | [»] | |
3HY2 | X-ray | 2.10 | A/B | 1-199 | [»] | |
4XCS | X-ray | 2.10 | A/B/C/D/E/F | 1-199 | [»] | |
SMRi | Q06830 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111089, 218 interactors |
CORUMi | Q06830 |
DIPi | DIP-33152N |
IntActi | Q06830, 100 interactors |
MINTi | Q06830 |
STRINGi | 9606.ENSP00000262746 |
Chemistry databases
ChEMBLi | CHEMBL5315 |
DrugBanki | DB11638, Artenimol DB09130, Copper DB09221, Polaprezinc DB01593, Zinc DB14487, Zinc acetate DB14533, Zinc chloride DB14548, Zinc sulfate, unspecified form |
Protein family/group databases
PeroxiBasei | 4501, Hs2CysPrx01 |
PTM databases
iPTMneti | Q06830 |
MetOSitei | Q06830 |
PhosphoSitePlusi | Q06830 |
SwissPalmi | Q06830 |
Genetic variation databases
BioMutai | PRDX1 |
DMDMi | 548453 |
2D gel databases
DOSAC-COBS-2DPAGEi | Q06830 |
OGPi | Q06830 |
SWISS-2DPAGEi | Q06830 |
UCD-2DPAGEi | Q06830 |
Proteomic databases
EPDi | Q06830 |
jPOSTi | Q06830 |
MassIVEi | Q06830 |
MaxQBi | Q06830 |
PaxDbi | Q06830 |
PeptideAtlasi | Q06830 |
PRIDEi | Q06830 |
ProteomicsDBi | 12697 58486 |
TopDownProteomicsi | Q06830 |
Protocols and materials databases
Antibodypediai | 1574, 666 antibodies |
DNASUi | 5052 |
Genome annotation databases
Ensembli | ENST00000262746; ENSP00000262746; ENSG00000117450 ENST00000319248; ENSP00000361152; ENSG00000117450 |
GeneIDi | 5052 |
KEGGi | hsa:5052 |
UCSCi | uc001coa.4, human |
Organism-specific databases
CTDi | 5052 |
DisGeNETi | 5052 |
GeneCardsi | PRDX1 |
HGNCi | HGNC:9352, PRDX1 |
HPAi | ENSG00000117450, Low tissue specificity |
MalaCardsi | PRDX1 |
MIMi | 176763, gene |
neXtProti | NX_Q06830 |
OpenTargetsi | ENSG00000117450 |
PharmGKBi | PA33722 |
VEuPathDBi | HostDB:ENSG00000117450.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0852, Eukaryota |
GeneTreei | ENSGT00940000154277 |
InParanoidi | Q06830 |
OMAi | FWYPKDF |
OrthoDBi | 1326484at2759 |
PhylomeDBi | Q06830 |
TreeFami | TF105181 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS04134-MONOMER |
BRENDAi | 1.11.1.15, 2681 |
PathwayCommonsi | Q06830 |
Reactomei | R-HSA-3299685, Detoxification of Reactive Oxygen Species R-HSA-5628897, TP53 Regulates Metabolic Genes R-HSA-8862803, Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models |
SIGNORi | Q06830 |
Miscellaneous databases
BioGRID-ORCSi | 5052, 50 hits in 890 CRISPR screens |
ChiTaRSi | PRDX1, human |
EvolutionaryTracei | Q06830 |
GeneWikii | Peroxiredoxin_1 |
GenomeRNAii | 5052 |
Pharosi | Q06830, Tbio |
PROi | PR:Q06830 |
RNActi | Q06830, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000117450, Expressed in thyroid gland and 241 other tissues |
ExpressionAtlasi | Q06830, baseline and differential |
Genevisiblei | Q06830, HS |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRDX1_HUMAN | |
Accessioni | Q06830Primary (citable) accession number: Q06830 Secondary accession number(s): B5BU26 Q5T155 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | February 10, 2021 | |
This is version 226 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families