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Entry version 160 (10 Feb 2021)
Sequence version 1 (01 Nov 1996)
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Protein

Glycine--tRNA ligase 2

Gene

GRS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PubMed:23816885).

Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis (By similarity).

By similarity1 Publication

Miscellaneous

Present with 2290 molecules/cell in log phase SD medium.1 Publication

Caution

GRS1, which appears to be a duplication of GRS2, is necessary and sufficient for both mitochondrial and cytoplasmic glycyl-tRNA synthetase activities, suggesting that GRS2 may not be essential.Curated

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 0.10 sec(-1) for the aminoacylation reaction (at 25 degrees Celsius).1 Publication
  1. KM=145 µM for glycine (at 25 degrees Celsius)1 Publication
  2. KM=0.53 µM for tRNA(Gly) (at 25 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei187GlycineBy similarity1
Binding sitei238GlycineBy similarity1
Binding sitei473ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi219 – 221ATPBy similarity3
Nucleotide bindingi230 – 231ATPBy similarity2
Nucleotide bindingi347 – 348ATPBy similarity2

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, Transferase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
6.1.1.14, 984

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Glycine--tRNA ligase 2 (EC:6.1.1.141 Publication)
Alternative name(s):
Diadenosine tetraphosphate synthetase (EC:2.7.7.-By similarity)
Short name:
Ap4A synthetase
Glycyl-tRNA synthetase 2
Short name:
GlyRS 2
Short name:
GlyRS2
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:GRS2
Ordered Locus Names:YPR081C
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XVI

Organism-specific databases

Saccharomyces Genome Database

More...
SGDi
S000006285, GRS2

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
FungiDB:YPR081C

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000730061 – 618Glycine--tRNA ligase 2Add BLAST618

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q06817

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q06817

PRoteomics IDEntifications database

More...
PRIDEi
Q06817

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

By similarity

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
36252, 58 interactors

Protein interaction database and analysis system

More...
IntActi
Q06817, 6 interactors

Molecular INTeraction database

More...
MINTi
Q06817

STRING: functional protein association networks

More...
STRINGi
4932.YPR081C

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
Q06817, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q06817

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni466 – 468Glycine bindingBy similarity3

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG2298, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000153759

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_015515_1_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q06817

Identification of Orthologs from Complete Genome Data

More...
OMAi
NDGFPAI

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00774, GlyRS-like_core, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.50.800, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002314, aa-tRNA-synt_IIb
IPR006195, aa-tRNA-synth_II
IPR004154, Anticodon-bd
IPR036621, Anticodon-bd_dom_sf
IPR027031, Gly-tRNA_synthase/POLG2
IPR033731, GlyRS-like_core
IPR002315, tRNA-synt_gly

The PANTHER Classification System

More...
PANTHERi
PTHR10745, PTHR10745, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03129, HGTP_anticodon, 1 hit
PF00587, tRNA-synt_2b, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR01043, TRNASYNTHGLY

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00389, glyS_dimeric, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50862, AA_TRNA_LIGASE_II, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q06817-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPLMSNSERD KLESTLRRRF FYTPSFEIYG GVSGLFDLGP PGCQLQNNLI
60 70 80 90 100
RLWREHFIME ENMLQVDGPM LTPYDVLKTS GHVDKFTDWM CRNPKTGEYY
110 120 130 140 150
RADHLIEQTL KKRLLDKDVN PQDMKNMEKI LTTIDGFSGP ELNLVMQEYN
160 170 180 190 200
INDPVTNDVL DALTSFNLMF ETKIGASGQL KAFLRPETAQ GQFLNFNKLL
210 220 230 240 250
EINQGKIPFA SASIGKSFRN EISPRSGLLR VREFLMAEIE HFVDPLNKSH
260 270 280 290 300
AKFNEVLNEE IPLLSRRLQE SGEVQLPVKM TIGEAVNSGM VENETLGYFM
310 320 330 340 350
ARVHQFLLNI GINKDKFRFR QHLKNEMAHY ATDCWDGEIL TSYGWIECVG
360 370 380 390 400
CADRAAFDLT VHSKKTGRSL TVKQKLDTPK ERTEWVVEVN KKFFGSKFKQ
410 420 430 440 450
KAKLIESVLS KFSQDELIRR HEELEKNGEF TCQVNGQIVK LDSSLVTIKM
460 470 480 490 500
KTTLQHIREY IPNVIEPSFG LGRIIYCIFD HCFQVRVDSE SRGFFSFPLQ
510 520 530 540 550
IAPIKVFVTT ISNNDGFPAI LKRISQALRK REIYFKIDDS NTSIGKKYAR
560 570 580 590 600
NDELGTPFGI TIDFETIKDQ TVTLRERNSM RQVRGTITDV ISTIDKMLHN
610
PDESDWDKST FGLSPVKI
Length:618
Mass (Da):71,018
Last modified:November 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i91D36F04D1AF7F38
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U51033 Genomic DNA Translation: AAB68130.1
BK006949 Genomic DNA Translation: DAA11499.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S69067

NCBI Reference Sequences

More...
RefSeqi
NP_015406.1, NM_001184178.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YPR081C_mRNA; YPR081C; YPR081C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
856196

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YPR081C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51033 Genomic DNA Translation: AAB68130.1
BK006949 Genomic DNA Translation: DAA11499.1
PIRiS69067
RefSeqiNP_015406.1, NM_001184178.1

3D structure databases

SMRiQ06817
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi36252, 58 interactors
IntActiQ06817, 6 interactors
MINTiQ06817
STRINGi4932.YPR081C

Proteomic databases

MaxQBiQ06817
PaxDbiQ06817
PRIDEiQ06817

Genome annotation databases

EnsemblFungiiYPR081C_mRNA; YPR081C; YPR081C
GeneIDi856196
KEGGisce:YPR081C

Organism-specific databases

SGDiS000006285, GRS2
VEuPathDBiFungiDB:YPR081C

Phylogenomic databases

eggNOGiKOG2298, Eukaryota
GeneTreeiENSGT00940000153759
HOGENOMiCLU_015515_1_0_1
InParanoidiQ06817
OMAiNDGFPAI

Enzyme and pathway databases

BRENDAi6.1.1.14, 984

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q06817
RNActiQ06817, protein

Family and domain databases

CDDicd00774, GlyRS-like_core, 1 hit
Gene3Di3.40.50.800, 1 hit
InterProiView protein in InterPro
IPR002314, aa-tRNA-synt_IIb
IPR006195, aa-tRNA-synth_II
IPR004154, Anticodon-bd
IPR036621, Anticodon-bd_dom_sf
IPR027031, Gly-tRNA_synthase/POLG2
IPR033731, GlyRS-like_core
IPR002315, tRNA-synt_gly
PANTHERiPTHR10745, PTHR10745, 1 hit
PfamiView protein in Pfam
PF03129, HGTP_anticodon, 1 hit
PF00587, tRNA-synt_2b, 1 hit
PRINTSiPR01043, TRNASYNTHGLY
TIGRFAMsiTIGR00389, glyS_dimeric, 1 hit
PROSITEiView protein in PROSITE
PS50862, AA_TRNA_LIGASE_II, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSYG2_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q06817
Secondary accession number(s): D6W483
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: February 10, 2021
This is version 160 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
  3. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  4. SIMILARITY comments
    Index of protein domains and families
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