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UniProtKB - Q06756 (ISPF_BACSU)
Protein
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Gene
ispF
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).
UniRule annotation1 PublicationCatalytic activityi
- 4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMPUniRule annotationEC:4.6.1.12UniRule annotation
Cofactori
a divalent metal cationUniRule annotationNote: Binds 1 divalent metal cation per subunit.UniRule annotation
: isopentenyl diphosphate biosynthesis via DXP pathway Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 9 | Divalent metal cationUniRule annotation | 1 | |
Metal bindingi | 11 | Divalent metal cation; via tele nitrogenUniRule annotation | 1 | |
Sitei | 35 | Transition state stabilizerUniRule annotation | 1 | |
Metal bindingi | 43 | Divalent metal cation; via pros nitrogenUniRule annotation | 1 | |
Sitei | 134 | Transition state stabilizerUniRule annotation | 1 | |
Binding sitei | 140 | 4-CDP-2-C-methyl-D-erythritol 2-phosphate; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 143 | 4-CDP-2-C-methyl-D-erythritol 2-phosphateUniRule annotation | 1 |
GO - Molecular functioni
- 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniPathway
- terpenoid biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Lyase |
Biological process | Isoprene biosynthesis |
Ligand | Metal-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU00910-MONOMER |
BRENDAi | 4.6.1.12, 658 |
UniPathwayi | UPA00056;UER00095 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthaseUniRule annotation (EC:4.6.1.12UniRule annotation)Short name: MECDP-synthaseUniRule annotation Short name: MECPP-synthaseUniRule annotation Short name: MECPSUniRule annotation |
Gene namesi | Name:ispFUniRule annotation Synonyms:yacN Ordered Locus Names:BSU00910 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene reveal a loss of rod shape, irregular septation, multicompartmentalized cells, and thickened cell walls. It also induces a decrease in isoprene production.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000189441 | 1 – 158 | 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthaseAdd BLAST | 158 |
Proteomic databases
PaxDbi | Q06756 |
PRIDEi | Q06756 |
Interactioni
Subunit structurei
Homotrimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 224308.BSU00910 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q06756 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 9 – 11 | 4-CDP-2-C-methyl-D-erythritol 2-phosphate bindingUniRule annotation | 3 | |
Regioni | 35 – 36 | 4-CDP-2-C-methyl-D-erythritol 2-phosphate bindingUniRule annotation | 2 | |
Regioni | 57 – 59 | 4-CDP-2-C-methyl-D-erythritol 2-phosphate bindingUniRule annotation | 3 | |
Regioni | 62 – 66 | 4-CDP-2-C-methyl-D-erythritol 2-phosphate bindingUniRule annotation | 5 | |
Regioni | 101 – 107 | 4-CDP-2-C-methyl-D-erythritol 2-phosphate bindingUniRule annotation | 7 | |
Regioni | 133 – 136 | 4-CDP-2-C-methyl-D-erythritol 2-phosphate bindingUniRule annotation | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0245, Bacteria |
InParanoidi | Q06756 |
OMAi | QHFGTGR |
PhylomeDBi | Q06756 |
Family and domain databases
CDDi | cd00554, MECDP_synthase, 1 hit |
Gene3Di | 3.30.1330.50, 1 hit |
HAMAPi | MF_00107, IspF, 1 hit |
InterProi | View protein in InterPro IPR003526, MECDP_synthase IPR020555, MECDP_synthase_CS IPR036571, MECDP_synthase_sf |
PANTHERi | PTHR43181, PTHR43181, 1 hit |
Pfami | View protein in Pfam PF02542, YgbB, 1 hit |
SUPFAMi | SSF69765, SSF69765, 1 hit |
TIGRFAMsi | TIGR00151, ispF, 1 hit |
PROSITEi | View protein in PROSITE PS01350, ISPF, 1 hit |
i Sequence
Sequence statusi: Complete.
Q06756-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFRIGQGFDV HQLVEGRPLI IGGIEIPYEK GLLGHSDADV LLHTVADACL
60 70 80 90 100
GAVGEGDIGK HFPDTDPEFK DADSFKLLQH VWGIVKQKGY VLGNIDCTII
110 120 130 140 150
AQKPKMLPYI EDMRKRIAEG LEADVSQVNV KATTTEKLGF TGRAEGIAAQ
ATVLIQKG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L14580 Genomic DNA Translation: AAA21795.1 D26185 Genomic DNA Translation: BAA05325.1 AL009126 Genomic DNA Translation: CAB11867.1 |
PIRi | F69741 |
RefSeqi | NP_387972.1, NC_000964.3 WP_003225745.1, NZ_JNCM01000029.1 |
Genome annotation databases
EnsemblBacteriai | CAB11867; CAB11867; BSU_00910 |
GeneIDi | 64301929 936634 |
KEGGi | bsu:BSU00910 |
PATRICi | fig|224308.179.peg.92 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L14580 Genomic DNA Translation: AAA21795.1 D26185 Genomic DNA Translation: BAA05325.1 AL009126 Genomic DNA Translation: CAB11867.1 |
PIRi | F69741 |
RefSeqi | NP_387972.1, NC_000964.3 WP_003225745.1, NZ_JNCM01000029.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5IWX | X-ray | 1.99 | A/B/C/D/E/F | 1-158 | [»] | |
5IWY | X-ray | 1.99 | A/B/C/D/E/F | 1-158 | [»] | |
SMRi | Q06756 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU00910 |
Proteomic databases
PaxDbi | Q06756 |
PRIDEi | Q06756 |
Genome annotation databases
EnsemblBacteriai | CAB11867; CAB11867; BSU_00910 |
GeneIDi | 64301929 936634 |
KEGGi | bsu:BSU00910 |
PATRICi | fig|224308.179.peg.92 |
Phylogenomic databases
eggNOGi | COG0245, Bacteria |
InParanoidi | Q06756 |
OMAi | QHFGTGR |
PhylomeDBi | Q06756 |
Enzyme and pathway databases
UniPathwayi | UPA00056;UER00095 |
BioCyci | BSUB:BSU00910-MONOMER |
BRENDAi | 4.6.1.12, 658 |
Family and domain databases
CDDi | cd00554, MECDP_synthase, 1 hit |
Gene3Di | 3.30.1330.50, 1 hit |
HAMAPi | MF_00107, IspF, 1 hit |
InterProi | View protein in InterPro IPR003526, MECDP_synthase IPR020555, MECDP_synthase_CS IPR036571, MECDP_synthase_sf |
PANTHERi | PTHR43181, PTHR43181, 1 hit |
Pfami | View protein in Pfam PF02542, YgbB, 1 hit |
SUPFAMi | SSF69765, SSF69765, 1 hit |
TIGRFAMsi | TIGR00151, ispF, 1 hit |
PROSITEi | View protein in PROSITE PS01350, ISPF, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ISPF_BACSU | |
Accessioni | Q06756Primary (citable) accession number: Q06756 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | September 29, 2021 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families