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UniProtKB - Q06755 (ISPD_BACSU)
Protein
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Gene
ispD
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).
By similarityCatalytic activityi
- EC:2.7.7.60
: isopentenyl diphosphate biosynthesis via DXP pathway Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate. This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 15 | Transition state stabilizerBy similarity | 1 | |
Sitei | 22 | Transition state stabilizerBy similarity | 1 | |
Sitei | 152 | Positions MEP for the nucleophilic attackBy similarity | 1 | |
Sitei | 209 | Positions MEP for the nucleophilic attackBy similarity | 1 |
GO - Molecular functioni
- 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Source: GO_Central
GO - Biological processi
- isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniPathway
- terpenoid biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Isoprene biosynthesis |
Enzyme and pathway databases
BioCyci | BSUB:BSU00900-MONOMER |
UniPathwayi | UPA00056;UER00093 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC:2.7.7.60)Alternative name(s): 4-diphosphocytidyl-2C-methyl-D-erythritol synthase MEP cytidylyltransferase Short name: MCT |
Gene namesi | Name:ispD Synonyms:yacM Ordered Locus Names:BSU00900 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075551 | 1 – 232 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferaseAdd BLAST | 232 |
Proteomic databases
PaxDbi | Q06755 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q06755 |
SMRi | Q06755 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1211, Bacteria |
InParanoidi | Q06755 |
OMAi | ERQHSVY |
PhylomeDBi | Q06755 |
Family and domain databases
CDDi | cd02516, CDP-ME_synthetase, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
HAMAPi | MF_00108, IspD, 1 hit |
InterProi | View protein in InterPro IPR001228, IspD IPR034683, IspD/TarI IPR018294, ISPD_synthase_CS IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF01128, IspD, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
TIGRFAMsi | TIGR00453, ispD, 1 hit |
PROSITEi | View protein in PROSITE PS01295, ISPD, 1 hit |
i Sequence
Sequence statusi: Complete.
Q06755-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSYDVVIPAA GQGKRMKAGR NKLFIELKGD PVIIHTLRVF DSHRQCDKII
60 70 80 90 100
LVINEQEREH FQQLLSDYPF QTSIELVAGG DERQHSVYKG LKAVKQEKIV
110 120 130 140 150
LVHDGARPFI KHEQIDELIA EAEQTGAAIL AVPVKDTIKR VQDLQVSETI
160 170 180 190 200
ERSSLWAVQT PQAFRLSLLM KAHAEAERKG FLGTDDASLV EQMEGGSVRV
210 220 230
VEGSYTNIKL TTPDDLTSAE AIMESESGNK HV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 57 | E → D in AAA21794 (PubMed:7510287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L14580 Genomic DNA Translation: AAA21794.1 D26185 Genomic DNA Translation: BAA05324.1 AL009126 Genomic DNA Translation: CAB11866.1 |
PIRi | S66119 |
RefSeqi | NP_387971.1, NC_000964.3 WP_003235019.1, NZ_JNCM01000029.1 |
Genome annotation databases
EnsemblBacteriai | CAB11866; CAB11866; BSU_00900 |
GeneIDi | 936855 |
KEGGi | bsu:BSU00900 |
PATRICi | fig|224308.179.peg.91 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L14580 Genomic DNA Translation: AAA21794.1 D26185 Genomic DNA Translation: BAA05324.1 AL009126 Genomic DNA Translation: CAB11866.1 |
PIRi | S66119 |
RefSeqi | NP_387971.1, NC_000964.3 WP_003235019.1, NZ_JNCM01000029.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5DDT | X-ray | 1.80 | A/B | 1-232 | [»] | |
5DDV | X-ray | 2.30 | A | 1-232 | [»] | |
5HS2 | X-ray | 1.90 | A/B | 1-232 | [»] | |
AlphaFoldDBi | Q06755 | |||||
SMRi | Q06755 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU00900 |
Proteomic databases
PaxDbi | Q06755 |
Genome annotation databases
EnsemblBacteriai | CAB11866; CAB11866; BSU_00900 |
GeneIDi | 936855 |
KEGGi | bsu:BSU00900 |
PATRICi | fig|224308.179.peg.91 |
Phylogenomic databases
eggNOGi | COG1211, Bacteria |
InParanoidi | Q06755 |
OMAi | ERQHSVY |
PhylomeDBi | Q06755 |
Enzyme and pathway databases
UniPathwayi | UPA00056;UER00093 |
BioCyci | BSUB:BSU00900-MONOMER |
Family and domain databases
CDDi | cd02516, CDP-ME_synthetase, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
HAMAPi | MF_00108, IspD, 1 hit |
InterProi | View protein in InterPro IPR001228, IspD IPR034683, IspD/TarI IPR018294, ISPD_synthase_CS IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF01128, IspD, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
TIGRFAMsi | TIGR00453, ispD, 1 hit |
PROSITEi | View protein in PROSITE PS01295, ISPD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ISPD_BACSU | |
Accessioni | Q06755Primary (citable) accession number: Q06755 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | August 4, 2003 | |
Last modified: | May 25, 2022 | |
This is version 140 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families