Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vacuolar protein-sorting-associated protein 36

Gene

VPS36

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ESCRT-II complex, which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Involved in the trafficking of the plasma membrane ATPase. Its ability to bind ubiquitin plays a central role in endosomal sorting of ubiquitinated cargo proteins by the ESCRT complexes.1 Publication

Miscellaneous

Present with 2470 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri114 – 151RanBP2-type 1; degenerateAdd BLAST38
Zinc fingeri177 – 205RanBP2-type 2Add BLAST29

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32478-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein-sorting-associated protein 36
Alternative name(s):
ESCRT-II complex subunit VPS36
Gene namesi
Name:VPS36
Synonyms:GRD12, VAC3, VPL11
Ordered Locus Names:YLR417W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR417W
SGDiS000004409 VPS36

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi187 – 188TF → GS: Abolishes ubiquitin-binding and vacuole sorting of ubiquitinated proteins. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002152271 – 566Vacuolar protein-sorting-associated protein 36Add BLAST566

Proteomic databases

MaxQBiQ06696
PaxDbiQ06696
PRIDEiQ06696

Interactioni

Subunit structurei

Component of the endosomal sorting required for transport complex II (ESCRT-II), which consists of 2 copies of VPS25, 1 copy of SNF8, and 1 copy of VPS36. The ESCRT-II complex interacts directly with the VPS20 subunit of the ESCRT-III complex. Binds ubiquitin.3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi31675, 151 interactors
ComplexPortaliCPX-1623 ESCRT-II complex
DIPiDIP-1744N
IntActiQ06696, 10 interactors
MINTiQ06696
STRINGi4932.YLR417W

Structurei

Secondary structure

1566
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ06696
SMRiQ06696
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06696

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 96GLUE N-terminalPROSITE-ProRule annotationAdd BLAST90
Domaini255 – 288GLUE C-terminalPROSITE-ProRule annotationAdd BLAST34

Domaini

The second RanBP2-type zinc-finger is functional and binds ubiquitin.1 Publication

Sequence similaritiesi

Belongs to the VPS36 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri114 – 151RanBP2-type 1; degenerateAdd BLAST38
Zinc fingeri177 – 205RanBP2-type 2Add BLAST29

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000067728
InParanoidiQ06696
KOiK12190
OMAiFYAGFLK
OrthoDBiEOG092C2NEC

Family and domain databases

Gene3Di1.10.10.10, 2 hits
2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR021648 GLUE_dom
IPR011993 PH-like_dom_sf
IPR037855 Vps36
IPR031558 Vps36-NZF-N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
IPR001876 Znf_RanBP2
IPR036443 Znf_RanBP2_sf
PANTHERiPTHR13128 PTHR13128, 1 hit
PfamiView protein in Pfam
PF16988 Vps36-NZF-N, 1 hit
PF11605 Vps36_ESCRT-II, 1 hit
SMARTiView protein in SMART
SM00547 ZnF_RBZ, 2 hits
SUPFAMiSSF46785 SSF46785, 1 hit
SSF90209 SSF90209, 1 hit
PROSITEiView protein in PROSITE
PS51495 GLUE, 1 hit

Sequencei

Sequence statusi: Complete.

Q06696-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEYWHYVETT SSGQPLLREG EKDIFIDQSV GLYHGKSKIL QRQRGRIFLT
60 70 80 90 100
SQRIIYIDDA KPTQNSLGLE LDDLAYVNYS SGFLTRSPRL ILFFKDPSSK
110 120 130 140 150
DELGKSAETA SADVVSTWVC PICMVSNETQ GEFTKDTLPT PICINCGVPA
160 170 180 190 200
DYELTKSSIN CSNAIDPNAN PQNQFGVNSE NICPACTFAN HPQIGNCEIC
210 220 230 240 250
GHRLPNASKV RSKLNRLNFH DSRVHIELEK NSLARNKSSH SALSSSSSTG
260 270 280 290 300
SSTEFVQLSF RKSDGVLFSQ ATERALENIL TEKNKHIFNQ NVVSVNGVDM
310 320 330 340 350
RKGASSHEYN NEVPFIETKL SRIGISSLEK SRENQLLNND ILFNNALTDL
360 370 380 390 400
NKLMSLATSI ERLYKNSNIT MKTKTLNLQD ESTVNEPKTR RPLLILDREK
410 420 430 440 450
FLNKELFLDE IAREIYEFTL SEFKDLNSDT NYMIITLVDL YAMYNKSMRI
460 470 480 490 500
GTGLISPMEM REACERFEHL GLNELKLVKV NKRILCVTSE KFDVVKEKLV
510 520 530 540 550
DLIGDNPGSD LLRLTQILSS NNSKSNWTLG ILMEVLQNCV DEGDLLIDKQ
560
LSGIYYYKNS YWPSHI
Length:566
Mass (Da):64,018
Last modified:November 1, 1996 - v1
Checksum:i60DDD4EA3A620409
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20162 Genomic DNA Translation: AAB67493.1
BK006945 Genomic DNA Translation: DAA09719.1
PIRiS59382
RefSeqiNP_013521.3, NM_001182305.3

Genome annotation databases

EnsemblFungiiYLR417W; YLR417W; YLR417W
GeneIDi851135
KEGGisce:YLR417W

Similar proteinsi

Entry informationi

Entry nameiVPS36_YEAST
AccessioniPrimary (citable) accession number: Q06696
Secondary accession number(s): D6VZ53
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 1, 1996
Last modified: September 12, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again