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Protein

Auxilin-like clathrin uncoating factor SWA2

Gene

SWA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cofactor for the uncoating of clathrin-coated vesicles (CCVs) by Hsp70-type chaperones (SSA1/2/3 and SSB1/2). Coat disassembly is important for fusion of vesicles with target membranes and for recycling components of clathrin coats to the cytoplasm for further rounds of vesicle formation. Binds to assembled clathrin and recruits the ATP-activated chaperone to CCVs. Stimulates the ATPase activity of the clathrin-associated Hsp70-type chaperone SSA1, which then disrupts clathrin-clathrin interactions, leading to release of the clathrin coat. In addition, prevents unproductive clathrin assembly in the cell. Also required for cortical endoplasmic reticulum inheritance.3 Publications

Miscellaneous

Present with 768 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • clathrin binding Source: SGD
  • ubiquitin binding Source: SGD

GO - Biological processi

  • clathrin coat disassembly Source: SGD
  • endoplasmic reticulum inheritance Source: SGD
  • positive regulation of ATPase activity Source: SGD
  • receptor-mediated endocytosis Source: GO_Central

Enzyme and pathway databases

BioCyciYEAST:G3O-29878-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Auxilin-like clathrin uncoating factor SWA2
Alternative name(s):
Bud site selection protein 24
DnaJ-related protein SWA2
Short name:
J protein SWA2
Synthetic lethal with ARF1 protein 2
Gene namesi
Name:SWA2
Synonyms:AUX1, BUD24
Ordered Locus Names:YDR320C
ORF Names:D9798.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR320C
SGDiS000002728 SWA2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi388G → R in SWA2-1/SWA2-TPR; partial loss of clathrin disassembly function. In SWA2-TPR-J; complete loss of clathrin disassembly function; when associated with 631-AAA-633. 2 Publications1
Mutagenesisi631 – 633HPD → AAA in SWA2-J; abolishes ATPase stimulation activity. In SWA2-TPR-J; complete loss of clathrin disassembly function; when associated with R-388. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002706211 – 668Auxilin-like clathrin uncoating factor SWA2Add BLAST668

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphoserineCombined sources1
Modified residuei64PhosphoserineCombined sources1
Modified residuei264PhosphoserineCombined sources1
Modified residuei308PhosphoserineCombined sources1
Modified residuei312PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ06677
PaxDbiQ06677
PRIDEiQ06677

PTM databases

iPTMnetiQ06677

Interactioni

Subunit structurei

Interacts with the clathrin light and heavy chains CLC1 and CHC1, respectively. Binds to clathrin with its N-terminal domain containing 3 clathrin-binding (CB) motifs. Association with clathrin is transient. Binds to polyubiquitin and ubiquitinated proteins.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • clathrin binding Source: SGD
  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi32376, 380 interactors
DIPiDIP-2847N
IntActiQ06677, 321 interactors
MINTiQ06677
STRINGi4932.YDR320C

Structurei

Secondary structure

1668
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi140 – 153Combined sources14
Helixi158 – 167Combined sources10
Helixi171 – 179Combined sources9

3D structure databases

ProteinModelPortaliQ06677
SMRiQ06677
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06677

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini140 – 180UBAAdd BLAST41
Repeati374 – 407TPR 1Add BLAST34
Repeati412 – 445TPR 2Add BLAST34
Repeati467 – 500TPR 3Add BLAST34
Domaini603 – 668JAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 100CB1Add BLAST100
Regioni238 – 302CB2Add BLAST65
Regioni303 – 362CB3Add BLAST60

Domaini

The TPR repeats and the J domain are required for interaction with Hsp70-type chaperones. The J domain is responsible for stimulating the ATPase activity of the chaperone.1 Publication

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

HOGENOMiHOG000001055
InParanoidiQ06677
OMAiSGYNEFK
OrthoDBiEOG092C1MUD

Family and domain databases

CDDicd14329 UBA_SWA2p_like, 1 hit
Gene3Di1.10.287.110, 1 hit
1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR036869 J_dom_sf
IPR015228 SWA2_UBA
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
IPR009060 UBA-like_sf
PfamiView protein in Pfam
PF09145 Ubiq-assoc, 1 hit
SMARTiView protein in SMART
SM00028 TPR, 3 hits
SUPFAMiSSF46565 SSF46565, 1 hit
SSF46934 SSF46934, 1 hit
SSF48452 SSF48452, 1 hit
PROSITEiView protein in PROSITE
PS50293 TPR_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q06677-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDPFAHLLT SLKNKDSASA SKETTPQSSN SPSITGSAVA DVARTDKSPN
60 70 80 90 100
DSLHSISAPP LIPSPKVDFS APPLVPTNST TKSNTANNTP PSALANTDDD
110 120 130 140 150
FNQLFGMGTV TTTDTIQKPD EDYYGSKEDH LYNGDDALVD EVKDMEIARL
160 170 180 190 200
MSLGLSIEEA TEFYENDVTY ERYLEILKSK QKERNDLAIR KKESGIKMEK
210 220 230 240 250
SGLSNIVGTD SNNLFSMATD FFNKGKKLVD QWTSFPPEAN DRLNNYSKTH
260 270 280 290 300
DKVEDYDLPQ VNDSPNRILF EDNEVVENLP PADNPDQDLL TDFETKIDIT
310 320 330 340 350
KRTAPDVSHS SSPTSGILIE ENSRRNEPLI EDSLLDFSEG NLTNSKSNED
360 370 380 390 400
STLFNENSNT DSTIPISDIE LSGYNEFKAK GTSLFKNGDY INSLQEYEKS
410 420 430 440 450
LNTLPLNHPL RIIALSNIIA SQLKIGEYSK SIENSSMALE LFPSSKAKWK
460 470 480 490 500
NKISNSDPER SFNDIWPKIM IRRAESFEHL ESFKKALETY QELIKKNFFD
510 520 530 540 550
DKIMQGKRRC QDFINPPPVK KSMPVKKKTT TTSPATKKQN LTASSSNSPI
560 570 580 590 600
SVDSTSEIKK RELENAKLAL YDKVFEKISS WKDGKDDDIR HLLANLSSLL
610 620 630 640 650
TWCNWKDVSM QDLVMPKRVK ITYMKAVAKT HPDKIPESLS LENKMIAENI
660
FSTLSIAWDK FKLQNDIN
Length:668
Mass (Da):75,020
Last modified:November 1, 1996 - v1
Checksum:iCCDF1F78315E3D44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32517 Genomic DNA Translation: AAB64756.1
BK006938 Genomic DNA Translation: DAA12161.1
PIRiS59786
RefSeqiNP_010606.1, NM_001180628.1

Genome annotation databases

EnsemblFungiiYDR320C; YDR320C; YDR320C
GeneIDi851918
KEGGisce:YDR320C

Similar proteinsi

Entry informationi

Entry nameiSWA2_YEAST
AccessioniPrimary (citable) accession number: Q06677
Secondary accession number(s): D6VSV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: July 18, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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