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UniProtKB - Q06651 (PIB1_YEAST)
Protein
E3 ubiquitin-protein ligase PIB1
Gene
PIB1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Functions as an E3 ubiquitin-protein ligase. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate.
2 PublicationsMiscellaneous
Present with 195 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 23 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 26 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 39 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 42 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 47 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 50 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 80 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 83 | Zinc 2PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 17 – 88 | FYVE-typePROSITE-ProRule annotationAdd BLAST | 72 | |
Zinc fingeri | 225 – 283 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 59 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- phosphatidylinositol-3-phosphate binding Source: SGD
- ubiquitin-protein transferase activity Source: SGD
GO - Biological processi
- negative regulation of gluconeogenesis Source: SGD
- protein ubiquitination Source: SGD
- regulation of protein catabolic process Source: SGD
- regulation of protein stability Source: SGD
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase PIB1 (EC:2.3.2.27)Alternative name(s): Phosphatidylinositol 3-phosphate-binding protein 1 RING-type E3 ubiquitin transferase PIB1Curated |
Gene namesi | Name:PIB1 Ordered Locus Names:YDR313C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002721, PIB1 |
VEuPathDBi | FungiDB:YDR313C |
Subcellular locationi
Vacuole
Endosome
Endosome
- endosome membrane Source: UniProtKB-SubCell
- late endosome Source: SGD
Vacuole
- fungal-type vacuole membrane Source: SGD
Keywords - Cellular componenti
Endosome, Membrane, VacuolePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 23 | C → S: Abolishes endosomal targeting. 1 Publication | 1 | |
Mutagenesisi | 225 | C → S: Abolishes E3 activity, strongly reduces zinc binding and destabilizes the protein, but no effect on subcellular location. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245839 | 1 – 286 | E3 ubiquitin-protein ligase PIB1Add BLAST | 286 |
Proteomic databases
MaxQBi | Q06651 |
PaxDbi | Q06651 |
PRIDEi | Q06651 |
PTM databases
iPTMneti | Q06651 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 32366, 94 interactors |
DIPi | DIP-1514N |
IntActi | Q06651, 3 interactors |
MINTi | Q06651 |
STRINGi | 4932.YDR313C |
Miscellaneous databases
RNActi | Q06651, protein |
Structurei
3D structure databases
AlphaFoldDBi | Q06651 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domaini
The FYVE domain mediates phosphatidylinositol 3-phosphate binding and is necessary and sufficient for targeting to endosome and vacuole membranes.
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 17 – 88 | FYVE-typePROSITE-ProRule annotationAdd BLAST | 72 | |
Zinc fingeri | 225 – 283 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 59 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1729, Eukaryota |
HOGENOMi | CLU_069851_0_0_1 |
InParanoidi | Q06651 |
OMAi | FCPLHDA |
Family and domain databases
Gene3Di | 3.30.40.10, 2 hits |
InterProi | View protein in InterPro IPR000306, Znf_FYVE IPR017455, Znf_FYVE-rel IPR011011, Znf_FYVE_PHD IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF01363, FYVE, 1 hit PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00064, FYVE, 1 hit SM00184, RING, 2 hits |
SUPFAMi | SSF57903, SSF57903, 1 hit |
PROSITEi | View protein in PROSITE PS50178, ZF_FYVE, 1 hit PS50089, ZF_RING_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q06651-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVIKEDCINN LARWQADEEA HSCFQCKTNF SFLVRRHHCR CCGRIFCSSC
60 70 80 90 100
TENFVNYNKK RVHALQKKNS DVESPPYRTC NECYDNLLHL NLLVSSTNRD
110 120 130 140 150
VRLSQTSVPP NALALSAPDS NTDEDAEILE DSVDQSGTAC RSEESSQNEE
160 170 180 190 200
DHFCPICNSD LTQFPDEEET RKHVEDCIQR AENAQQHTNT SDAADDSVKE
210 220 230 240 250
SPAFQNRMLV YKISPNTTDN AIKECPICFE NMEPGEKVGR LECLCVFHYK
260 270 280
CIKNWFHKRA QMTAAQKGNG HAFVKRNFCP FHDAVF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 166 | D → G in AAS56068 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28374 Genomic DNA Translation: AAB64749.1 AY557742 Genomic DNA Translation: AAS56068.1 BK006938 Genomic DNA Translation: DAA12152.1 |
PIRi | S61199 |
RefSeqi | NP_010599.1, NM_001180621.1 |
Genome annotation databases
EnsemblFungii | YDR313C_mRNA; YDR313C; YDR313C |
GeneIDi | 851908 |
KEGGi | sce:YDR313C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28374 Genomic DNA Translation: AAB64749.1 AY557742 Genomic DNA Translation: AAS56068.1 BK006938 Genomic DNA Translation: DAA12152.1 |
PIRi | S61199 |
RefSeqi | NP_010599.1, NM_001180621.1 |
3D structure databases
AlphaFoldDBi | Q06651 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 32366, 94 interactors |
DIPi | DIP-1514N |
IntActi | Q06651, 3 interactors |
MINTi | Q06651 |
STRINGi | 4932.YDR313C |
PTM databases
iPTMneti | Q06651 |
Proteomic databases
MaxQBi | Q06651 |
PaxDbi | Q06651 |
PRIDEi | Q06651 |
Genome annotation databases
EnsemblFungii | YDR313C_mRNA; YDR313C; YDR313C |
GeneIDi | 851908 |
KEGGi | sce:YDR313C |
Organism-specific databases
SGDi | S000002721, PIB1 |
VEuPathDBi | FungiDB:YDR313C |
Phylogenomic databases
eggNOGi | KOG1729, Eukaryota |
HOGENOMi | CLU_069851_0_0_1 |
InParanoidi | Q06651 |
OMAi | FCPLHDA |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
PROi | PR:Q06651 |
RNActi | Q06651, protein |
Family and domain databases
Gene3Di | 3.30.40.10, 2 hits |
InterProi | View protein in InterPro IPR000306, Znf_FYVE IPR017455, Znf_FYVE-rel IPR011011, Znf_FYVE_PHD IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF01363, FYVE, 1 hit PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00064, FYVE, 1 hit SM00184, RING, 2 hits |
SUPFAMi | SSF57903, SSF57903, 1 hit |
PROSITEi | View protein in PROSITE PS50178, ZF_FYVE, 1 hit PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PIB1_YEAST | |
Accessioni | Q06651Primary (citable) accession number: Q06651 Secondary accession number(s): D6VSU2, Q6Q5S5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways