ARR3

Functionⁱ

Plasma membrane transporter that confers resistance to toxic metalloids by mediating extrusion of arsenite (As(III)) and antimonite (Sb(III)) out of cells. Displays low-affinity As(III)/H⁺ and Sb(III)/H⁺ exchange activity.8 Publications

Kineticsⁱ

Vmax for arsenite is approximately 3 times higher than for antimonite.1 Publication

K_M=
2 mM for arsenite export
1 Publication
Manual assertion based on experiment inⁱ
- Ref.6
  "Acr3p is a plasma membrane antiporter that catalyzes As(III)/H(+) and Sb(III)/H(+) exchange in Saccharomyces cerevisiae."
  Maciaszczyk-Dziubinska E., Migocka M., Wysocki R.
  Biochim. Biophys. Acta 1808:1855-1859(2011) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
K_M=
2 mM for antimonite export
1 Publication
Manual assertion based on experiment inⁱ
- Ref.6
  "Acr3p is a plasma membrane antiporter that catalyzes As(III)/H(+) and Sb(III)/H(+) exchange in Saccharomyces cerevisiae."
  Maciaszczyk-Dziubinska E., Migocka M., Wysocki R.
  Biochim. Biophys. Acta 1808:1855-1859(2011) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.

GO - Molecular functionⁱ

antimonite transmembrane transporter activity
Source: SGD
Inferred from mutant phenotypeⁱ
- 20655873
antiporter activity
Source: SGD
Inferred from mutant phenotypeⁱ
- 21447319
arsenite transmembrane transporter activity
Source: SGD
Inferred from direct assayⁱ
- 24291645

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

antimonite transport
Source: SGD
Inferred from mutant phenotypeⁱ
- 20655873
arsenite transport
Source: SGD
Inferred from mutant phenotypeⁱ
- 24291645
response to arsenic-containing substance Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Antiport, Arsenical resistance, Ion transport, Transport

Biological process

Antiport, Arsenical resistance, Ion transport, Transport

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-34321-MONOMER

BioCycⁱ

YEAST:G3O-34321-MONOMER

Protein family/group databases

TCDBⁱ	2.A.59.1.1 the arsenical resistance-3 (acr3) family

TCDBⁱ

2.A.59.1.1 the arsenical resistance-3 (acr3) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Arsenical-resistance protein 3Curated Alternative name(s): Arsenic compounds resistance protein 31 Publication As(III)/H(+) and Sb(III)/H(+)antiporterCurated
Gene namesⁱ	Name:ARR3Imported Synonyms:ACR31 Publication Ordered Locus Names:YPR201WImported ORF Names:P9677.2
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome XVI

Organism-specific databases

EuPathDBⁱ	FungiDB:YPR201W
Saccharomyces Genome Database More...SGDⁱ	S000006405 ARR3

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 34	Cytoplasmic1 PublicationAdd BLAST	34
Transmembraneⁱ	35 – 55	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	56 – 69	Extracellular1 PublicationAdd BLAST	14
Transmembraneⁱ	70 – 90	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	91 – 113	Cytoplasmic1 PublicationAdd BLAST	23
Transmembraneⁱ	114 – 134	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	135 – 141	Extracellular1 Publication	7
Transmembraneⁱ	142 – 162	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	163 – 169	Cytoplasmic1 Publication	7
Transmembraneⁱ	170 – 190	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	191 – 216	Extracellular1 PublicationAdd BLAST	26
Transmembraneⁱ	217 – 237	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	238 – 245	Cytoplasmic1 Publication	8
Transmembraneⁱ	246 – 266	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	267 – 280	Extracellular1 PublicationAdd BLAST	14
Transmembraneⁱ	281 – 301	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	302 – 343	Cytoplasmic1 PublicationAdd BLAST	42
Transmembraneⁱ	344 – 364	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	365 – 369	Extracellular1 Publication	5
Transmembraneⁱ	370 – 390	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	391 – 404	Cytoplasmic1 PublicationAdd BLAST	14

Keywords - Cellular componentⁱ

Cell membrane, Membrane

Pathology & Biotechⁱ

Biotechnological useⁱ

Heterologous expression endows plants with greater arsenic resistance by enhancing arsenite efflux. Reduces arsenic accumulation in rice grains (PubMed:22107880). Does not lower significantly arsenic tissue levels in Arabidopsis (PubMed:22380876).2 Publications

Disruption phenotypeⁱ

Leads to sensitivity to antimony, tellurite, cadmium, and phenylarsine oxide.1 Publication

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	90	C → A: Leads to ER retention and arsenite sensitivity. 1 Publication	1
Mutagenesisⁱ	117	N → A: Impairs cell membrane localization, and leads to arsenite sensitivity. 1 Publication	1
Mutagenesisⁱ	150	R → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication	1
Mutagenesisⁱ	151	C → V: Leads to complete loss of metalloid transport function. 1 Publication	1
Mutagenesisⁱ	158	W → A: Impairs cell membrane localization, and leads to arsenite sensitivity. 1 Publication	1
Mutagenesisⁱ	169	C → A: Greatly reduces arsenite efflux. 1 Publication	1
Mutagenesisⁱ	176	N → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication	1
Mutagenesisⁱ	192	C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication	1
Mutagenesisⁱ	230	R → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication	1
Mutagenesisⁱ	266	F → A: Impairs arsenite resistance. 1 Publication	1
Mutagenesisⁱ	283	C → A: Does not alter the arsenite/proton exchange across the plasma membrane. 1 Publication	1
Mutagenesisⁱ	290	Y → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication	1
Mutagenesisⁱ	316	C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication	1
Mutagenesisⁱ	318	C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication	1
Mutagenesisⁱ	333	C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication	1
Mutagenesisⁱ	344	C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication	1
Mutagenesisⁱ	345	F → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication	1
Mutagenesisⁱ	349	S → A: Impairs arsenite resistance. 1 Publication	1
Mutagenesisⁱ	351	N → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication	1
Mutagenesisⁱ	352	F → A: Impairs arsenite resistance. 1 Publication	1
Mutagenesisⁱ	353	E → A: Impairs arsenite resistance. 1 Publication	1
Mutagenesisⁱ	380	E → A: Impairs arsenite resistance. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000064440	1 – 404	Arsenical-resistance protein 3Add BLAST		404

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Glycosylationⁱ	201	N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation		1
Glycosylationⁱ	365	N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation		1

Keywords - PTMⁱ

Glycoprotein

Proteomic databases

PaxDbⁱ	Q06598

PaxDbⁱ

Q06598

Expressionⁱ

Inductionⁱ

Expression is highly induced by arsenite and antimonite.1 Publication

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	36371, 90 interactors
Database of interacting proteins More...DIPⁱ	DIP-3797N
IntActⁱ	Q06598, 3 interactors
Molecular INTeraction database More...MINTⁱ	Q06598
STRINGⁱ	4932.YPR201W

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q06598
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the arsenical resistance-3 (ACR3) (TC 2.A.59) family. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidⁱ	Q06598
KEGG Orthology (KO) More...KOⁱ	K03325
OMAⁱ	CTAMVLM
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C3QM8

Family and domain databases

InterProⁱ	View protein in InterPro IPR004706 Arsenical-R_Acr3 IPR002657 BilAc:Na_symport/Acr3
PANTHERⁱ	PTHR43057 PTHR43057, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01758 SBF, 1 hit
PIRSFⁱ	PIRSF005508 Acr3, 1 hit
TIGRFAMsⁱ	TIGR00832 acr3, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q06598-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSEDQKSENS VPSKVNMVNR TDILTTIKSL SWLDLMLPFT IILSIIIAVI 
        60         70         80         90        100
ISVYVPSSRH TFDAEGHPNL MGVSIPLTVG MIVMMIPPIC KVSWESIHKY 
       110        120        130        140        150
FYRSYIRKQL ALSLFLNWVI GPLLMTALAW MALFDYKEYR QGIIMIGVAR 
       160        170        180        190        200
CIAMVLIWNQ IAGGDNDLCV VLVITNSLLQ MVLYAPLQIF YCYVISHDHL 
       210        220        230        240        250
NTSNRVLFEE VAKSVGVFLG IPLGIGIIIR LGSLTIAGKS NYEKYILRFI 
       260        270        280        290        300
SPWAMIGFHY TLFVIFISRG YQFIHEIGSA ILCFVPLVLY FFIAWFLTFA 
       310        320        330        340        350
LMRYLSISRS DTQRECSCDQ ELLLKRVWGR KSCEASFSIT MTQCFTMASN 
       360        370        380        390        400
NFELSLAIAI SLYGNNSKQA IAATFGPLLE VPILLILAIV ARILKPYYIW 

NNRN

Length:404

Mass (Da):45,848

Last modified:November 1, 1996 - v1

Checksum:ⁱ1BE290E26772B4CA

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U25841 Genomic DNA Translation: AAB64629.1 BK006949 Genomic DNA Translation: DAA11615.1
PIRⁱ	S58830
NCBI Reference Sequences More...RefSeqⁱ	NP_015527.1, NM_001184298.1

Genome annotation databases

EnsemblFungiⁱ	YPR201W; YPR201W; YPR201W
GeneIDⁱ	856331
KEGGⁱ	sce:YPR201W

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q06598	Arr3p		YEAS8		404	UniRef100_Q06598
Arr3p		SACCK		404
ARR3p Plasma membrane metalloid/H+ antiporter		Saccharomyces sp. 'boulardii'		404
Arr3p		YEASC		404
Arr3p		YEASL		404
+2

Protein	Similar proteins		Species	Score	Length	Source
Q06598	Arr3p		YEAS8		404	UniRef90_Q06598
Arr3p		SACCK		404
ARR3p Plasma membrane metalloid/H+ antiporter		Saccharomyces sp. 'boulardii'		404
Arr3p		YEASC		404
Arr3p		YEASL		404
+10

Protein	Similar proteins		Species	Score	Length	Source
Q06598	Arr3p		YEAS8		404	UniRef50_Q06598
Arr3p		SACCK		404
ARR3p Plasma membrane metalloid/H+ antiporter		Saccharomyces sp. 'boulardii'		404
Arr3p		YEASC		404
Arr3p		YEASL		404
+32

Entry informationⁱ

Entry nameⁱ	ARR3_YEAST
Accessionⁱ	Q06598Primary (citable) accession number: Q06598 Secondary accession number(s): D6W4J9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	November 1, 1996
	Last modified:	September 12, 2018
	This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q06598 (ARR3_YEAST)

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Arsenical-resistance protein 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the use of a specific protein in the biotechnological industry.<p><a href='/help/biotechnological_use' target='_top'>More...</a></p>Biotechnological usei

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi