UniProtKB - Q06598 (ARR3_YEAST)
Protein
Arsenical-resistance protein 3
Gene
ARR3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Plasma membrane transporter that confers resistance to toxic metalloids by mediating extrusion of arsenite (As(III)) and antimonite (Sb(III)) out of cells. Displays low-affinity As(III)/H+ and Sb(III)/H+ exchange activity.8 Publications
Kineticsi
Vmax for arsenite is approximately 3 times higher than for antimonite.1 Publication
- KM=2 mM for arsenite export1 Publication
- KM=2 mM for antimonite export1 Publication
GO - Molecular functioni
- antimonite transmembrane transporter activity Source: SGD
- antiporter activity Source: SGD
- arsenite transmembrane transporter activity Source: SGD
GO - Biological processi
- antimonite transport Source: SGD
- arsenite transport Source: SGD
- response to arsenic-containing substance Source: UniProtKB-KW
Keywordsi
Biological process | Antiport, Arsenical resistance, Ion transport, Transport |
Protein family/group databases
TCDBi | 2.A.59.1.1, the arsenical resistance-3 (acr3) family |
Names & Taxonomyi
Protein namesi | Recommended name: Arsenical-resistance protein 3CuratedAlternative name(s): Arsenic compounds resistance protein 31 Publication As(III)/H(+) and Sb(III)/H(+)antiporterCurated |
Gene namesi | Ordered Locus Names:YPR201WImported ORF Names:P9677.2 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006405, ARR3 |
VEuPathDBi | FungiDB:YPR201W |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication4 Publications; Multi-pass membrane protein Sequence analysis1 Publication
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 34 | Cytoplasmic1 PublicationAdd BLAST | 34 | |
Transmembranei | 35 – 55 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 56 – 69 | Extracellular1 PublicationAdd BLAST | 14 | |
Transmembranei | 70 – 90 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 91 – 113 | Cytoplasmic1 PublicationAdd BLAST | 23 | |
Transmembranei | 114 – 134 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 135 – 141 | Extracellular1 Publication | 7 | |
Transmembranei | 142 – 162 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 163 – 169 | Cytoplasmic1 Publication | 7 | |
Transmembranei | 170 – 190 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 191 – 216 | Extracellular1 PublicationAdd BLAST | 26 | |
Transmembranei | 217 – 237 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 238 – 245 | Cytoplasmic1 Publication | 8 | |
Transmembranei | 246 – 266 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 267 – 280 | Extracellular1 PublicationAdd BLAST | 14 | |
Transmembranei | 281 – 301 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 302 – 343 | Cytoplasmic1 PublicationAdd BLAST | 42 | |
Transmembranei | 344 – 364 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 365 – 369 | Extracellular1 Publication | 5 | |
Transmembranei | 370 – 390 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 391 – 404 | Cytoplasmic1 PublicationAdd BLAST | 14 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Biotechnological usei
Heterologous expression endows plants with greater arsenic resistance by enhancing arsenite efflux. Reduces arsenic accumulation in rice grains (PubMed:22107880). Does not lower significantly arsenic tissue levels in Arabidopsis (PubMed:22380876).2 Publications
Disruption phenotypei
Leads to sensitivity to antimony, tellurite, cadmium, and phenylarsine oxide.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 90 | C → A: Leads to ER retention and arsenite sensitivity. 1 Publication | 1 | |
Mutagenesisi | 117 | N → A: Impairs cell membrane localization, and leads to arsenite sensitivity. 1 Publication | 1 | |
Mutagenesisi | 150 | R → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication | 1 | |
Mutagenesisi | 151 | C → V: Leads to complete loss of metalloid transport function. 1 Publication | 1 | |
Mutagenesisi | 158 | W → A: Impairs cell membrane localization, and leads to arsenite sensitivity. 1 Publication | 1 | |
Mutagenesisi | 169 | C → A: Greatly reduces arsenite efflux. 1 Publication | 1 | |
Mutagenesisi | 176 | N → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication | 1 | |
Mutagenesisi | 192 | C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication | 1 | |
Mutagenesisi | 230 | R → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication | 1 | |
Mutagenesisi | 266 | F → A: Impairs arsenite resistance. 1 Publication | 1 | |
Mutagenesisi | 283 | C → A: Does not alter the arsenite/proton exchange across the plasma membrane. 1 Publication | 1 | |
Mutagenesisi | 290 | Y → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication | 1 | |
Mutagenesisi | 316 | C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication | 1 | |
Mutagenesisi | 318 | C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication | 1 | |
Mutagenesisi | 333 | C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication | 1 | |
Mutagenesisi | 344 | C → A: Results in moderate reduction of arsenite transport capacities and sorting perturbations. 1 Publication | 1 | |
Mutagenesisi | 345 | F → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication | 1 | |
Mutagenesisi | 349 | S → A: Impairs arsenite resistance. 1 Publication | 1 | |
Mutagenesisi | 351 | N → A: Leads to ER retention, protein unstability and arsenite sensitivity. 1 Publication | 1 | |
Mutagenesisi | 352 | F → A: Impairs arsenite resistance. 1 Publication | 1 | |
Mutagenesisi | 353 | E → A: Impairs arsenite resistance. 1 Publication | 1 | |
Mutagenesisi | 380 | E → A: Impairs arsenite resistance. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064440 | 1 – 404 | Arsenical-resistance protein 3Add BLAST | 404 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 201 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 365 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q06598 |
PTM databases
iPTMneti | Q06598 |
Expressioni
Inductioni
Expression is highly induced by arsenite and antimonite.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 36371, 91 interactors |
DIPi | DIP-3797N |
IntActi | Q06598, 3 interactors |
MINTi | Q06598 |
STRINGi | 4932.YPR201W |
Miscellaneous databases
RNActi | Q06598, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QPKH, Eukaryota |
GeneTreei | ENSGT00950000182808 |
HOGENOMi | CLU_022869_0_0_1 |
InParanoidi | Q06598 |
OMAi | CTAMVLM |
Family and domain databases
InterProi | View protein in InterPro IPR004706, Arsenical-R_Acr3 IPR002657, BilAc:Na_symport/Acr3 |
PANTHERi | PTHR43057, PTHR43057, 1 hit |
Pfami | View protein in Pfam PF01758, SBF, 1 hit |
PIRSFi | PIRSF005508, Acr3, 1 hit |
TIGRFAMsi | TIGR00832, acr3, 1 hit |
i Sequence
Sequence statusi: Complete.
Q06598-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEDQKSENS VPSKVNMVNR TDILTTIKSL SWLDLMLPFT IILSIIIAVI
60 70 80 90 100
ISVYVPSSRH TFDAEGHPNL MGVSIPLTVG MIVMMIPPIC KVSWESIHKY
110 120 130 140 150
FYRSYIRKQL ALSLFLNWVI GPLLMTALAW MALFDYKEYR QGIIMIGVAR
160 170 180 190 200
CIAMVLIWNQ IAGGDNDLCV VLVITNSLLQ MVLYAPLQIF YCYVISHDHL
210 220 230 240 250
NTSNRVLFEE VAKSVGVFLG IPLGIGIIIR LGSLTIAGKS NYEKYILRFI
260 270 280 290 300
SPWAMIGFHY TLFVIFISRG YQFIHEIGSA ILCFVPLVLY FFIAWFLTFA
310 320 330 340 350
LMRYLSISRS DTQRECSCDQ ELLLKRVWGR KSCEASFSIT MTQCFTMASN
360 370 380 390 400
NFELSLAIAI SLYGNNSKQA IAATFGPLLE VPILLILAIV ARILKPYYIW
NNRN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25841 Genomic DNA Translation: AAB64629.1 BK006949 Genomic DNA Translation: DAA11615.1 |
PIRi | S58830 |
RefSeqi | NP_015527.1, NM_001184298.1 |
Genome annotation databases
EnsemblFungii | YPR201W_mRNA; YPR201W; YPR201W |
GeneIDi | 856331 |
KEGGi | sce:YPR201W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U25841 Genomic DNA Translation: AAB64629.1 BK006949 Genomic DNA Translation: DAA11615.1 |
PIRi | S58830 |
RefSeqi | NP_015527.1, NM_001184298.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 36371, 91 interactors |
DIPi | DIP-3797N |
IntActi | Q06598, 3 interactors |
MINTi | Q06598 |
STRINGi | 4932.YPR201W |
Protein family/group databases
TCDBi | 2.A.59.1.1, the arsenical resistance-3 (acr3) family |
PTM databases
iPTMneti | Q06598 |
Proteomic databases
PaxDbi | Q06598 |
Genome annotation databases
EnsemblFungii | YPR201W_mRNA; YPR201W; YPR201W |
GeneIDi | 856331 |
KEGGi | sce:YPR201W |
Organism-specific databases
SGDi | S000006405, ARR3 |
VEuPathDBi | FungiDB:YPR201W |
Phylogenomic databases
eggNOGi | ENOG502QPKH, Eukaryota |
GeneTreei | ENSGT00950000182808 |
HOGENOMi | CLU_022869_0_0_1 |
InParanoidi | Q06598 |
OMAi | CTAMVLM |
Miscellaneous databases
PROi | PR:Q06598 |
RNActi | Q06598, protein |
Family and domain databases
InterProi | View protein in InterPro IPR004706, Arsenical-R_Acr3 IPR002657, BilAc:Na_symport/Acr3 |
PANTHERi | PTHR43057, PTHR43057, 1 hit |
Pfami | View protein in Pfam PF01758, SBF, 1 hit |
PIRSFi | PIRSF005508, Acr3, 1 hit |
TIGRFAMsi | TIGR00832, acr3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARR3_YEAST | |
Accessioni | Q06598Primary (citable) accession number: Q06598 Secondary accession number(s): D6W4J9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 160 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - SIMILARITY comments
Index of protein domains and families