UniProtKB - Q06587 (RING1_HUMAN)
E3 ubiquitin-protein ligase RING1
RING1
Functioni
Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
1 PublicationMiscellaneous
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 48 – 88 | RING-typePROSITE-ProRule annotationAdd BLAST | 41 |
GO - Molecular functioni
- chromatin binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: GO_Central
- ubiquitin-protein transferase activator activity Source: Ensembl
GO - Biological processi
- anterior/posterior pattern specification Source: Ensembl
- camera-type eye morphogenesis Source: Ensembl
- chromatin organization Source: UniProtKB-KW
- histone H2A monoubiquitination Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
Keywordsi
Molecular function | Chromatin regulator, Repressor, Transferase |
Biological process | Transcription, Transcription regulation, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q06587 |
Reactomei | R-HSA-2559580, Oxidative Stress Induced Senescence R-HSA-3108214, SUMOylation of DNA damage response and repair proteins R-HSA-3899300, SUMOylation of transcription cofactors R-HSA-4551638, SUMOylation of chromatin organization proteins R-HSA-4570464, SUMOylation of RNA binding proteins R-HSA-4655427, SUMOylation of DNA methylation proteins R-HSA-8939243, RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known R-HSA-8943724, Regulation of PTEN gene transcription R-HSA-8953750, Transcriptional Regulation by E2F6 |
SignaLinki | Q06587 |
SIGNORi | Q06587 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase RING1 (EC:2.3.2.27)Alternative name(s): Polycomb complex protein RING1 RING finger protein 1 RING-type E3 ubiquitin transferase RING1Curated Really interesting new gene 1 protein |
Gene namesi | Name:RING1 Synonyms:RNF1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10018, RING1 |
MIMi | 602045, gene |
neXtProti | NX_Q06587 |
VEuPathDBi | HostDB:ENSG00000204227 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
- Nucleus speckle 1 Publication
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear speck Source: UniProtKB-SubCell
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
- PcG protein complex Source: UniProtKB
- PRC1 complex Source: UniProtKB
Other locations
- sex chromatin Source: Ensembl
- ubiquitin ligase complex Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 6015 |
OpenTargetsi | ENSG00000204227 |
PharmGKBi | PA34393 |
Miscellaneous databases
Pharosi | Q06587, Tbio |
Genetic variation databases
BioMutai | RING1 |
DMDMi | 90110053 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056384 | 1 – 406 | E3 ubiquitin-protein ligase RING1Add BLAST | 406 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 24 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 38 | PhosphoserineCombined sources | 1 | |
Modified residuei | 140 | PhosphoserineCombined sources | 1 | |
Modified residuei | 187 | PhosphoserineCombined sources | 1 | |
Modified residuei | 190 | PhosphoserineCombined sources | 1 | |
Modified residuei | 215 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 220 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 229 | PhosphoserineCombined sources | 1 | |
Modified residuei | 232 | PhosphoserineCombined sources | 1 | |
Modified residuei | 248 | PhosphoserineCombined sources | 1 | |
Modified residuei | 254 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q06587 |
jPOSTi | Q06587 |
MassIVEi | Q06587 |
MaxQBi | Q06587 |
PaxDbi | Q06587 |
PeptideAtlasi | Q06587 |
PRIDEi | Q06587 |
ProteomicsDBi | 58462 [Q06587-1] 58463 [Q06587-2] |
TopDownProteomicsi | Q06587-1 [Q06587-1] Q06587-2 [Q06587-2] |
PTM databases
GlyGeni | Q06587, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q06587 |
PhosphoSitePlusi | Q06587 |
Expressioni
Gene expression databases
Bgeei | ENSG00000204227, Expressed in olfactory bulb and 246 other tissues |
ExpressionAtlasi | Q06587, baseline and differential |
Genevisiblei | Q06587, HS |
Organism-specific databases
HPAi | ENSG00000204227, Low tissue specificity |
Interactioni
Subunit structurei
Component of chromatin-associated Polycomb (PcG) complexes.
Interacts with BMI1 (By similarity). Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2 MBLR, L3MBTL2 and YAF2.
Interacts with CBX2 and PCGF6.
Component of a PRC1-like complex.
Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RNF2/RING2.
Interacts with PCGF2, RNF2; CBX6, CBX7 and CBX8.
Interacts with PHC2 (By similarity).
Interacts with MN1 (PubMed:31839203).
By similarity1 PublicationBinary interactionsi
Q06587
Isoform 2 [Q06587-2]
With | #Exp. | IntAct |
---|---|---|
RYBP [Q8N488] | 3 | EBI-7065222,EBI-752324 |
Protein-protein interaction databases
BioGRIDi | 111947, 140 interactors |
CORUMi | Q06587 |
DIPi | DIP-42043N |
IntActi | Q06587, 115 interactors |
MINTi | Q06587 |
STRINGi | 9606.ENSP00000363787 |
Miscellaneous databases
RNActi | Q06587, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 30 – 234 | Necessary for transcriptional repressionBy similarityAdd BLAST | 205 | |
Regioni | 148 – 263 | DisorderedSequence analysisAdd BLAST | 116 | |
Regioni | 230 – 406 | Necessary for interaction with CBX2By similarityAdd BLAST | 177 | |
Regioni | 309 – 354 | DisorderedSequence analysisAdd BLAST | 46 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 201 – 204 | Nuclear localization signalSequence analysis | 4 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 48 – 88 | RING-typePROSITE-ProRule annotationAdd BLAST | 41 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0311, Eukaryota |
GeneTreei | ENSGT00940000161022 |
HOGENOMi | CLU_056557_1_0_1 |
InParanoidi | Q06587 |
OMAi | MIVGEVL |
PhylomeDBi | Q06587 |
TreeFami | TF105501 |
Family and domain databases
CDDi | cd16739, RING-HC_RING1, 1 hit |
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR032443, RAWUL IPR043540, RING1/RING2 IPR042741, RING1_RING-HC IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR46076, PTHR46076, 1 hit |
Pfami | View protein in Pfam PF16207, RAWUL, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTTPANAQNA SKTWELSLYE LHRTPQEAIM DGTEIAVSPR SLHSELMCPI
60 70 80 90 100
CLDMLKNTMT TKECLHRFCS DCIVTALRSG NKECPTCRKK LVSKRSLRPD
110 120 130 140 150
PNFDALISKI YPSREEYEAH QDRVLIRLSR LHNQQALSSS IEEGLRMQAM
160 170 180 190 200
HRAQRVRRPI PGSDQTTTMS GGEGEPGEGE GDGEDVSSDS APDSAPGPAP
210 220 230 240 250
KRPRGGGAGG SSVGTGGGGT GGVGGGAGSE DSGDRGGTLG GGTLGPPSPP
260 270 280 290 300
GAPSPPEPGG EIELVFRPHP LLVEKGEYCQ TRYVKTTGNA TVDHLSKYLA
310 320 330 340 350
LRIALERRQQ QEAGEPGGPG GGASDTGGPD GCGGEGGGAG GGDGPEEPAL
360 370 380 390 400
PSLEGVSEKQ YTIYIAPGGG AFTTLNGSLT LELVNEKFWK VSRPLELCYA
PTKDPK
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_076618 | 95 | R → Q1 PublicationCorresponds to variant dbSNP:rs1204881780Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_017694 | 1 – 29 | Missing in isoform 2. 2 PublicationsAdd BLAST | 29 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z14000 mRNA Translation: CAA78389.1 BT007352 mRNA Translation: AAP36016.1 AK289355 mRNA Translation: BAF82044.1 AL031228 Genomic DNA Translation: CAA20235.1 AL031228 Genomic DNA Translation: CAI95620.1 AL645940 Genomic DNA No translation available. AL713971 Genomic DNA No translation available. AL844527 Genomic DNA No translation available. CR547129 Genomic DNA No translation available. CR759733 Genomic DNA No translation available. CR759786 Genomic DNA No translation available. CR847841 Genomic DNA No translation available. BC002922 mRNA Translation: AAH02922.2 BC051866 mRNA Translation: AAH51866.1 |
CCDSi | CCDS34424.1 [Q06587-1] |
PIRi | A47380 |
RefSeqi | NP_002922.2, NM_002931.3 [Q06587-1] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z14000 mRNA Translation: CAA78389.1 BT007352 mRNA Translation: AAP36016.1 AK289355 mRNA Translation: BAF82044.1 AL031228 Genomic DNA Translation: CAA20235.1 AL031228 Genomic DNA Translation: CAI95620.1 AL645940 Genomic DNA No translation available. AL713971 Genomic DNA No translation available. AL844527 Genomic DNA No translation available. CR547129 Genomic DNA No translation available. CR759733 Genomic DNA No translation available. CR759786 Genomic DNA No translation available. CR847841 Genomic DNA No translation available. BC002922 mRNA Translation: AAH02922.2 BC051866 mRNA Translation: AAH51866.1 |
CCDSi | CCDS34424.1 [Q06587-1] |
PIRi | A47380 |
RefSeqi | NP_002922.2, NM_002931.3 [Q06587-1] |
3D structure databases
AlphaFoldDBi | Q06587 |
SMRi | Q06587 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 111947, 140 interactors |
CORUMi | Q06587 |
DIPi | DIP-42043N |
IntActi | Q06587, 115 interactors |
MINTi | Q06587 |
STRINGi | 9606.ENSP00000363787 |
PTM databases
GlyGeni | Q06587, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q06587 |
PhosphoSitePlusi | Q06587 |
Genetic variation databases
BioMutai | RING1 |
DMDMi | 90110053 |
Proteomic databases
EPDi | Q06587 |
jPOSTi | Q06587 |
MassIVEi | Q06587 |
MaxQBi | Q06587 |
PaxDbi | Q06587 |
PeptideAtlasi | Q06587 |
PRIDEi | Q06587 |
ProteomicsDBi | 58462 [Q06587-1] 58463 [Q06587-2] |
TopDownProteomicsi | Q06587-1 [Q06587-1] Q06587-2 [Q06587-2] |
Protocols and materials databases
Antibodypediai | 1772, 564 antibodies from 36 providers |
DNASUi | 6015 |
Genome annotation databases
Organism-specific databases
CTDi | 6015 |
DisGeNETi | 6015 |
GeneCardsi | RING1 |
HGNCi | HGNC:10018, RING1 |
HPAi | ENSG00000204227, Low tissue specificity |
MIMi | 602045, gene |
neXtProti | NX_Q06587 |
OpenTargetsi | ENSG00000204227 |
PharmGKBi | PA34393 |
VEuPathDBi | HostDB:ENSG00000204227 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0311, Eukaryota |
GeneTreei | ENSGT00940000161022 |
HOGENOMi | CLU_056557_1_0_1 |
InParanoidi | Q06587 |
OMAi | MIVGEVL |
PhylomeDBi | Q06587 |
TreeFami | TF105501 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q06587 |
Reactomei | R-HSA-2559580, Oxidative Stress Induced Senescence R-HSA-3108214, SUMOylation of DNA damage response and repair proteins R-HSA-3899300, SUMOylation of transcription cofactors R-HSA-4551638, SUMOylation of chromatin organization proteins R-HSA-4570464, SUMOylation of RNA binding proteins R-HSA-4655427, SUMOylation of DNA methylation proteins R-HSA-8939243, RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known R-HSA-8943724, Regulation of PTEN gene transcription R-HSA-8953750, Transcriptional Regulation by E2F6 |
SignaLinki | Q06587 |
SIGNORi | Q06587 |
Miscellaneous databases
BioGRID-ORCSi | 6015, 25 hits in 1130 CRISPR screens |
ChiTaRSi | RING1, human |
GeneWikii | RING1 |
GenomeRNAii | 6015 |
Pharosi | Q06587, Tbio |
PROi | PR:Q06587 |
RNActi | Q06587, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000204227, Expressed in olfactory bulb and 246 other tissues |
ExpressionAtlasi | Q06587, baseline and differential |
Genevisiblei | Q06587, HS |
Family and domain databases
CDDi | cd16739, RING-HC_RING1, 1 hit |
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR032443, RAWUL IPR043540, RING1/RING2 IPR042741, RING1_RING-HC IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR46076, PTHR46076, 1 hit |
Pfami | View protein in Pfam PF16207, RAWUL, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RING1_HUMAN | |
Accessioni | Q06587Primary (citable) accession number: Q06587 Secondary accession number(s): A8JZZ0 Q86V19 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | March 21, 2006 | |
Last modified: | May 25, 2022 | |
This is version 208 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways