RpS3

Functionⁱ

Has DNA repair activity directed towards the mutagenic lesions 8-oxoguanine and abasic sites in DNA. It can cleave DNA containing 8-oxoguanine residues efficiently. Also acts as an ap lyase, cleaving phosphodiester bonds via a beta,delta elimination reaction.

GO - Molecular functionⁱ

class I DNA-(apurinic or apyrimidinic site) endonuclease activity
Source: FlyBase
Inferred from direct assayⁱ
- "Drosophila ribosomal protein S3 contains an activity that cleaves DNA at apurinic/apyrimidinic sites."
  Wilson D.M., Deutsch W.A., Kelley M.R.
  J. Biol. Chem. 269:25359-25364(1994) [PubMed] [Europe PMC] [Abstract]
- Ref.6
  "A Drosophila ribosomal protein contains 8-oxoguanine and abasic site DNA repair activities."
  Yacoub A., Augeri L., Kelley M.R., Doetsch P.W., Deutsch W.A.
  EMBO J. 15:2306-2312(1996) [PubMed] [Europe PMC] [Abstract]
  Cited for: DNA REPAIR ACTIVITY, SUBCELLULAR LOCATION.
damaged DNA binding
Source: UniProtKB
Inferred from direct assayⁱ
- "Characterization of human ribosomal protein S3 binding to 7,8-dihydro-8-oxoguanine and abasic sites by surface plasmon resonance."
  Hegde V., Wang M., Deutsch W.A.
  DNA Repair 3:121-126(2004) [PubMed] [Europe PMC] [Abstract]
DNA-(apurinic or apyrimidinic site) endonuclease activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
  Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
  Brief. Bioinformatics 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
endonuclease activity Source: UniProtKB-KW
oxidized purine DNA binding
Source: UniProtKB
Inferred from direct assayⁱ
- "Characterization of human ribosomal protein S3 binding to 7,8-dihydro-8-oxoguanine and abasic sites by surface plasmon resonance."
  Hegde V., Wang M., Deutsch W.A.
  DNA Repair 3:121-126(2004) [PubMed] [Europe PMC] [Abstract]
oxidized purine nucleobase lesion DNA N-glycosylase activity
Source: FlyBase
Inferred from direct assayⁱ
- Ref.6
  "A Drosophila ribosomal protein contains 8-oxoguanine and abasic site DNA repair activities."
  Yacoub A., Augeri L., Kelley M.R., Doetsch P.W., Deutsch W.A.
  EMBO J. 15:2306-2312(1996) [PubMed] [Europe PMC] [Abstract]
  Cited for: DNA REPAIR ACTIVITY, SUBCELLULAR LOCATION.
RNA binding Source: UniProtKB-KW
structural constituent of ribosome
Source: FlyBase
Inferred from high throughput direct assayⁱ
- "Characterization of the Drosophila melanogaster ribosomal proteome."
  Alonso J., Santaren J.F.
  J. Proteome Res. 5:2025-2032(2006) [PubMed] [Europe PMC] [Abstract]

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Endonuclease, Hydrolase, Nuclease, Ribonucleoprotein, Ribosomal protein, RNA-binding
Biological process	DNA damage, DNA repair

Enzyme and pathway databases

Reactomeⁱ	R-DME-156827 L13a-mediated translational silencing of Ceruloplasmin expression R-DME-1799339 SRP-dependent cotranslational protein targeting to membrane R-DME-6791226 Major pathway of rRNA processing in the nucleolus and cytosol R-DME-72649 Translation initiation complex formation R-DME-72689 Formation of a pool of free 40S subunits R-DME-72695 Formation of the ternary complex, and subsequently, the 43S complex R-DME-72702 Ribosomal scanning and start codon recognition R-DME-72706 GTP hydrolysis and joining of the 60S ribosomal subunit R-DME-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-DME-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Reactomeⁱ

R-DME-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-DME-1799339 SRP-dependent cotranslational protein targeting to membrane
R-DME-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
R-DME-72649 Translation initiation complex formation
R-DME-72689 Formation of a pool of free 40S subunits
R-DME-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-DME-72702 Ribosomal scanning and start codon recognition
R-DME-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-DME-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-DME-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Protein family/group databases

MoonProtⁱ	Q06559

MoonProtⁱ

Q06559

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 40S ribosomal protein S3
Gene namesⁱ	Name:RpS3 Synonyms:M(3)95A ORF Names:CG6779
Organismⁱ	Drosophila melanogaster (Fruit fly)
Taxonomic identifierⁱ	7227 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophila › Sophophora › melanogaster group › melanogaster subgroup
Proteomesⁱ	UP000000803 Componentⁱ: Chromosome 3R

Organism-specific databases

Drosophila genome database More...FlyBaseⁱ	FBgn0002622 RpS3

FlyBaseⁱ

FBgn0002622 RpS3

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000130328	1 – 246	40S ribosomal protein S3Add BLAST		246

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	223	Phosphothreonine1 Publication	1
Modified residueⁱ	226	Phosphothreonine1 Publication	1
Modified residueⁱ	241	Phosphoserine1 Publication	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	Q06559
PRIDEⁱ	Q06559

PTM databases

iPTMnetⁱ	Q06559

iPTMnetⁱ

Q06559

Expressionⁱ

Gene expression databases

Bgeeⁱ	FBgn0002622 Expressed in 33 organ(s), highest expression level in eye disc (Drosophila)
ExpressionAtlasⁱ	Q06559 differential
Genevisibleⁱ	Q06559 DM

Interactionⁱ

Subunit structureⁱ

Interacts with LTV1; the interaction is RNA-independent.

1 Publication

Protein-protein interaction databases

BioGridⁱ	67703, 179 interactors
Database of interacting proteins More...DIPⁱ	DIP-17168N
IntActⁱ	Q06559, 3 interactors
Molecular INTeraction database More...MINTⁱ	Q06559
STRINGⁱ	7227.FBpp0083802

Structureⁱ

3D structure databases

SMRⁱ	Q06559
ModBaseⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	23 – 94	KH type-2PROSITE-ProRule annotationAdd BLAST		72

Sequence similaritiesⁱ

Belongs to the universal ribosomal protein uS3 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG3181 Eukaryota COG0092 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000008610
InParanoidⁱ	Q06559
KEGG Orthology (KO) More...KOⁱ	K02985
OMAⁱ	MLPHDPE
Database of Orthologous Groups More...OrthoDBⁱ	1135751at2759
PhylomeDBⁱ	Q06559

Family and domain databases

Gene3Dⁱ	3.30.1140.32, 1 hit 3.30.300.20, 1 hit
InterProⁱ	View protein in InterPro IPR015946 KH_dom-like_a/b IPR004044 KH_dom_type_2 IPR009019 KH_sf_prok-type IPR001351 Ribosomal_S3_C IPR036419 Ribosomal_S3_C_sf IPR018280 Ribosomal_S3_CS IPR005703 Ribosomal_S3_euk/arc
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07650 KH_2, 1 hit PF00189 Ribosomal_S3_C, 1 hit
SUPFAMⁱ	SSF54814 SSF54814, 1 hit SSF54821 SSF54821, 1 hit
TIGRFAMsⁱ	TIGR01008 uS3_euk_arch, 1 hit
PROSITEⁱ	View protein in PROSITE PS50823 KH_TYPE_2, 1 hit PS00548 RIBOSOMAL_S3, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q06559-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNANLPISKK RKFVSDGIFK AELNEFLTRE LAEDGYSGVE VRVTPSRTEI 
        60         70         80         90        100
IIMATKTQQV LGEKGRRIRE LTAMVQKRFN FETGRIELYA EKVAARGLCA 
       110        120        130        140        150
IAQAESLRYK LTGGLAVRRA CYGVLRYIME SGAKGCEVVV SGKLRGQRAK 
       160        170        180        190        200
SMKFVDGLMI HSGDPCNDYV ETATRHVLLR QGVLGIKVKV MLPYDPKNKI 
       210        220        230        240 
GPKKPLPDNV SVVEPKEEKI YETPETEYKI PPPSKPLDDL SEAKVL

Length:246

Mass (Da):27,471

Last modified:June 1, 1994 - v1

Checksum:ⁱ122A6E775339E673

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	G3M3A2	G3M3A2_DROME		Ribosomal protein S3, isoform B Ribosomal protein S3, isoform B, EC 3.2.2.23, EC 4.2.99.18 (RpS3-PA)	RpS3 6779, chr3R:19185518..19185701, Dmel\CG6779, DmRpS3, dS3	246	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	46 – 47	SR → FG in CAA51425 (PubMed:8070662).Curated	2
Sequence conflictⁱ	62	G → A in CAA51425 (PubMed:8070662).Curated	1
Sequence conflictⁱ	114	G → E in CAA51425 (PubMed:8070662).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L13690 mRNA Translation: AAA28875.1 X72921 Genomic DNA Translation: CAA51425.1 AE014297 Genomic DNA Translation: AAF56129.1 AY118971 mRNA Translation: AAM50831.1
PIRⁱ	S35620
NCBI Reference Sequences More...RefSeqⁱ	NP_001287481.1, NM_001300552.1 NP_476632.1, NM_057284.6

Genome annotation databases

EnsemblMetazoaⁱ	FBtr0084410; FBpp0083802; FBgn0002622 FBtr0346349; FBpp0312066; FBgn0002622
GeneIDⁱ	42761
KEGGⁱ	dme:Dmel_CG6779

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q06559	IP15838p (Fragment)		DROME		253	UniRef100_Q06559
Ribosomal protein S3, isoform B		DROME		246

Protein	Similar proteins		Species	Score	Length	Source
Q06559	IP15838p (Fragment)		DROME		253	UniRef90_Q06559
Uncharacterized protein (Fragment)		DROPS		206
Ribosomal protein S3, isoform B		DROME		246

Protein	Similar proteins		Species	Score	Length	Source
Q06559	IP15838p (Fragment)		DROME		253	UniRef50_Q06559
Uncharacterized protein (Fragment)		DROPS		206
Ribosomal protein S3		Kockovaella imperatae		253
Uncharacterized protein		RAMVA		258
Uncharacterized protein		VITBC		262
+62

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L13690 mRNA Translation: AAA28875.1 X72921 Genomic DNA Translation: CAA51425.1 AE014297 Genomic DNA Translation: AAF56129.1 AY118971 mRNA Translation: AAM50831.1
PIRⁱ	S35620
RefSeqⁱ	NP_001287481.1, NM_001300552.1 NP_476632.1, NM_057284.6

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4V6W	electron microscopy	6.00	AD	1-246	[»]
SMRⁱ	Q06559
ModBaseⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	67703, 179 interactors
DIPⁱ	DIP-17168N
IntActⁱ	Q06559, 3 interactors
MINTⁱ	Q06559
STRINGⁱ	7227.FBpp0083802

Protein family/group databases

MoonProtⁱ	Q06559

MoonProtⁱ

Q06559

PTM databases

iPTMnetⁱ	Q06559

iPTMnetⁱ

Q06559

Proteomic databases

PaxDbⁱ	Q06559
PRIDEⁱ	Q06559

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblMetazoaⁱ	FBtr0084410; FBpp0083802; FBgn0002622 FBtr0346349; FBpp0312066; FBgn0002622
GeneIDⁱ	42761
KEGGⁱ	dme:Dmel_CG6779

Organism-specific databases

CTDⁱ	6188
FlyBaseⁱ	FBgn0002622 RpS3

Phylogenomic databases

eggNOGⁱ	KOG3181 Eukaryota COG0092 LUCA
GeneTreeⁱ	ENSGT00390000008610
InParanoidⁱ	Q06559
KOⁱ	K02985
OMAⁱ	MLPHDPE
OrthoDBⁱ	1135751at2759
PhylomeDBⁱ	Q06559

Enzyme and pathway databases

Reactomeⁱ	R-DME-156827 L13a-mediated translational silencing of Ceruloplasmin expression R-DME-1799339 SRP-dependent cotranslational protein targeting to membrane R-DME-6791226 Major pathway of rRNA processing in the nucleolus and cytosol R-DME-72649 Translation initiation complex formation R-DME-72689 Formation of a pool of free 40S subunits R-DME-72695 Formation of the ternary complex, and subsequently, the 43S complex R-DME-72702 Ribosomal scanning and start codon recognition R-DME-72706 GTP hydrolysis and joining of the 60S ribosomal subunit R-DME-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-DME-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Reactomeⁱ

R-DME-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-DME-1799339 SRP-dependent cotranslational protein targeting to membrane
R-DME-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
R-DME-72649 Translation initiation complex formation
R-DME-72689 Formation of a pool of free 40S subunits
R-DME-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-DME-72702 Ribosomal scanning and start codon recognition
R-DME-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-DME-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-DME-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Miscellaneous databases

ChiTaRSⁱ	RpS3 fly
GenomeRNAiⁱ	42761
Protein Ontology More...PROⁱ	PR:Q06559

Gene expression databases

Bgeeⁱ	FBgn0002622 Expressed in 33 organ(s), highest expression level in eye disc (Drosophila)
ExpressionAtlasⁱ	Q06559 differential
Genevisibleⁱ	Q06559 DM

Family and domain databases

Gene3Dⁱ	3.30.1140.32, 1 hit 3.30.300.20, 1 hit
InterProⁱ	View protein in InterPro IPR015946 KH_dom-like_a/b IPR004044 KH_dom_type_2 IPR009019 KH_sf_prok-type IPR001351 Ribosomal_S3_C IPR036419 Ribosomal_S3_C_sf IPR018280 Ribosomal_S3_CS IPR005703 Ribosomal_S3_euk/arc
Pfamⁱ	View protein in Pfam PF07650 KH_2, 1 hit PF00189 Ribosomal_S3_C, 1 hit
SUPFAMⁱ	SSF54814 SSF54814, 1 hit SSF54821 SSF54821, 1 hit
TIGRFAMsⁱ	TIGR01008 uS3_euk_arch, 1 hit
PROSITEⁱ	View protein in PROSITE PS50823 KH_TYPE_2, 1 hit PS00548 RIBOSOMAL_S3, 1 hit
ProtoNetⁱ	Search...
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RS3_DROME
Accessionⁱ	Q06559Primary (citable) accession number: Q06559 Secondary accession number(s): Q9VCM9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
	Last sequence update:	June 1, 1994
	Last modified:	July 3, 2019
	This is version 182 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Drosophila annotation project

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UniProtKB - Q06559 (RS3_DROME)

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40S ribosomal protein S3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cytosol

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ