UniProtKB - Q06559 (RS3_DROME)
Protein
40S ribosomal protein S3
Gene
RpS3
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Has DNA repair activity directed towards the mutagenic lesions 8-oxoguanine and abasic sites in DNA. It can cleave DNA containing 8-oxoguanine residues efficiently. Also acts as an ap lyase, cleaving phosphodiester bonds via a beta,delta elimination reaction.
GO - Molecular functioni
- class I DNA-(apurinic or apyrimidinic site) endonuclease activity Source: FlyBase
- damaged DNA binding Source: UniProtKB
- endonuclease activity Source: UniProtKB-KW
- oxidized purine DNA binding Source: UniProtKB
- oxidized purine nucleobase lesion DNA N-glycosylase activity Source: FlyBase
- RNA binding Source: UniProtKB-KW
- structural constituent of ribosome Source: FlyBase
GO - Biological processi
- cytoplasmic translation Source: FlyBase
- DNA repair Source: FlyBase
- mitotic cell cycle Source: FlyBase
- negative regulation of neuron apoptotic process Source: FlyBase
- positive regulation of apoptotic signaling pathway Source: GO_Central
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease, Ribonucleoprotein, Ribosomal protein, RNA-binding |
Biological process | DNA damage, DNA repair |
Enzyme and pathway databases
Reactomei | R-DME-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-DME-1799339, SRP-dependent cotranslational protein targeting to membrane R-DME-72649, Translation initiation complex formation R-DME-72689, Formation of a pool of free 40S subunits R-DME-72695, Formation of the ternary complex, and subsequently, the 43S complex R-DME-72702, Ribosomal scanning and start codon recognition R-DME-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-DME-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-DME-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Protein family/group databases
MoonProti | Q06559 |
Names & Taxonomyi
Protein namesi | Recommended name: 40S ribosomal protein S3 |
Gene namesi | Name:RpS3 Synonyms:M(3)95A ORF Names:CG6779 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0002622, RpS3 |
Subcellular locationi
Cytosol
- cytosolic ribosome Source: FlyBase
- cytosolic small ribosomal subunit Source: GO_Central
Nucleus
- nuclear matrix Source: FlyBase
- nucleus Source: FlyBase
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000130328 | 1 – 246 | 40S ribosomal protein S3Add BLAST | 246 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 223 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 226 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 241 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q06559 |
PRIDEi | Q06559 |
PTM databases
iPTMneti | Q06559 |
Expressioni
Gene expression databases
Bgeei | FBgn0002622, Expressed in eye disc (Drosophila) and 40 other tissues |
ExpressionAtlasi | Q06559, baseline and differential |
Genevisiblei | Q06559, DM |
Interactioni
Subunit structurei
Interacts with LTV1; the interaction is RNA-independent.
1 PublicationProtein-protein interaction databases
BioGRIDi | 67703, 179 interactors |
DIPi | DIP-17168N |
IntActi | Q06559, 3 interactors |
MINTi | Q06559 |
STRINGi | 7227.FBpp0083802 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 23 – 94 | KH type-2PROSITE-ProRule annotationAdd BLAST | 72 |
Sequence similaritiesi
Belongs to the universal ribosomal protein uS3 family.Curated
Phylogenomic databases
eggNOGi | KOG3181, Eukaryota |
GeneTreei | ENSGT00390000008610 |
HOGENOMi | CLU_058591_2_1_1 |
InParanoidi | Q06559 |
OMAi | MLPHDPE |
OrthoDBi | 1135751at2759 |
PhylomeDBi | Q06559 |
Family and domain databases
Gene3Di | 3.30.1140.32, 1 hit 3.30.300.20, 1 hit |
InterProi | View protein in InterPro IPR015946, KH_dom-like_a/b IPR004044, KH_dom_type_2 IPR009019, KH_sf_prok-type IPR001351, Ribosomal_S3_C IPR036419, Ribosomal_S3_C_sf IPR018280, Ribosomal_S3_CS IPR005703, Ribosomal_S3_euk/arc |
Pfami | View protein in Pfam PF07650, KH_2, 1 hit PF00189, Ribosomal_S3_C, 1 hit |
SUPFAMi | SSF54814, SSF54814, 1 hit SSF54821, SSF54821, 1 hit |
TIGRFAMsi | TIGR01008, uS3_euk_arch, 1 hit |
PROSITEi | View protein in PROSITE PS50823, KH_TYPE_2, 1 hit PS00548, RIBOSOMAL_S3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q06559-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNANLPISKK RKFVSDGIFK AELNEFLTRE LAEDGYSGVE VRVTPSRTEI
60 70 80 90 100
IIMATKTQQV LGEKGRRIRE LTAMVQKRFN FETGRIELYA EKVAARGLCA
110 120 130 140 150
IAQAESLRYK LTGGLAVRRA CYGVLRYIME SGAKGCEVVV SGKLRGQRAK
160 170 180 190 200
SMKFVDGLMI HSGDPCNDYV ETATRHVLLR QGVLGIKVKV MLPYDPKNKI
210 220 230 240
GPKKPLPDNV SVVEPKEEKI YETPETEYKI PPPSKPLDDL SEAKVL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3M3A2 | G3M3A2_DROME | Ribosomal protein S3, isoform B | RpS3 6779, chr3R:19185518..19185701, Dmel\CG6779, DmRpS3, dS3 | 246 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 46 – 47 | SR → FG in CAA51425 (PubMed:8070662).Curated | 2 | |
Sequence conflicti | 62 | G → A in CAA51425 (PubMed:8070662).Curated | 1 | |
Sequence conflicti | 114 | G → E in CAA51425 (PubMed:8070662).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13690 mRNA Translation: AAA28875.1 X72921 Genomic DNA Translation: CAA51425.1 AE014297 Genomic DNA Translation: AAF56129.1 AY118971 mRNA Translation: AAM50831.1 |
PIRi | S35620 |
RefSeqi | NP_001287481.1, NM_001300552.1 NP_476632.1, NM_057284.6 |
Genome annotation databases
EnsemblMetazoai | FBtr0084410; FBpp0083802; FBgn0002622 FBtr0346349; FBpp0312066; FBgn0002622 |
GeneIDi | 42761 |
KEGGi | dme:Dmel_CG6779 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13690 mRNA Translation: AAA28875.1 X72921 Genomic DNA Translation: CAA51425.1 AE014297 Genomic DNA Translation: AAF56129.1 AY118971 mRNA Translation: AAM50831.1 |
PIRi | S35620 |
RefSeqi | NP_001287481.1, NM_001300552.1 NP_476632.1, NM_057284.6 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4V6W | electron microscopy | 6.00 | AD | 1-246 | [»] | |
SMRi | Q06559 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 67703, 179 interactors |
DIPi | DIP-17168N |
IntActi | Q06559, 3 interactors |
MINTi | Q06559 |
STRINGi | 7227.FBpp0083802 |
Protein family/group databases
MoonProti | Q06559 |
PTM databases
iPTMneti | Q06559 |
Proteomic databases
PaxDbi | Q06559 |
PRIDEi | Q06559 |
Genome annotation databases
EnsemblMetazoai | FBtr0084410; FBpp0083802; FBgn0002622 FBtr0346349; FBpp0312066; FBgn0002622 |
GeneIDi | 42761 |
KEGGi | dme:Dmel_CG6779 |
Organism-specific databases
CTDi | 6188 |
FlyBasei | FBgn0002622, RpS3 |
Phylogenomic databases
eggNOGi | KOG3181, Eukaryota |
GeneTreei | ENSGT00390000008610 |
HOGENOMi | CLU_058591_2_1_1 |
InParanoidi | Q06559 |
OMAi | MLPHDPE |
OrthoDBi | 1135751at2759 |
PhylomeDBi | Q06559 |
Enzyme and pathway databases
Reactomei | R-DME-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-DME-1799339, SRP-dependent cotranslational protein targeting to membrane R-DME-72649, Translation initiation complex formation R-DME-72689, Formation of a pool of free 40S subunits R-DME-72695, Formation of the ternary complex, and subsequently, the 43S complex R-DME-72702, Ribosomal scanning and start codon recognition R-DME-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-DME-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-DME-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
BioGRID-ORCSi | 42761, 0 hits in 1 CRISPR screen |
ChiTaRSi | RpS3, fly |
GenomeRNAii | 42761 |
PROi | PR:Q06559 |
Gene expression databases
Bgeei | FBgn0002622, Expressed in eye disc (Drosophila) and 40 other tissues |
ExpressionAtlasi | Q06559, baseline and differential |
Genevisiblei | Q06559, DM |
Family and domain databases
Gene3Di | 3.30.1140.32, 1 hit 3.30.300.20, 1 hit |
InterProi | View protein in InterPro IPR015946, KH_dom-like_a/b IPR004044, KH_dom_type_2 IPR009019, KH_sf_prok-type IPR001351, Ribosomal_S3_C IPR036419, Ribosomal_S3_C_sf IPR018280, Ribosomal_S3_CS IPR005703, Ribosomal_S3_euk/arc |
Pfami | View protein in Pfam PF07650, KH_2, 1 hit PF00189, Ribosomal_S3_C, 1 hit |
SUPFAMi | SSF54814, SSF54814, 1 hit SSF54821, SSF54821, 1 hit |
TIGRFAMsi | TIGR01008, uS3_euk_arch, 1 hit |
PROSITEi | View protein in PROSITE PS50823, KH_TYPE_2, 1 hit PS00548, RIBOSOMAL_S3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RS3_DROME | |
Accessioni | Q06559Primary (citable) accession number: Q06559 Secondary accession number(s): Q9VCM9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 190 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Ribosomal proteins
Ribosomal proteins families and list of entries - Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families