UniProtKB - Q06518 (NOS2_RAT)
Protein
Nitric oxide synthase, inducible
Gene
Nos2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.By similarity
Caution
sequence Was originally thought to originate from human but appears to be from rat.1 Publication
Catalytic activityi
- EC:1.14.13.39
Cofactori
Protein has several cofactor binding sites:- heme
- FADNote: Binds 1 FAD.
- FMNNote: Binds 1 FMN.
- (6R)-L-erythro-5,6,7,8-tetrahydrobiopterinNote: Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
Activity regulationi
Not stimulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 107 | ZincBy similarity | 1 | |
Metal bindingi | 112 | ZincBy similarity | 1 | |
Metal bindingi | 197 | Iron (heme axial ligand)By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 620 – 651 | FMNPROSITE-ProRule annotationAdd BLAST | 32 | |
Nucleotide bindingi | 764 – 775 | FADBy similarityAdd BLAST | 12 | |
Nucleotide bindingi | 900 – 910 | FADBy similarityAdd BLAST | 11 | |
Nucleotide bindingi | 975 – 993 | NADPBy similarityAdd BLAST | 19 | |
Nucleotide bindingi | 1073 – 1088 | NADPBy similarityAdd BLAST | 16 |
GO - Molecular functioni
- actin binding Source: RGD
- arginine binding Source: RGD
- beta-catenin binding Source: RGD
- cadherin binding Source: RGD
- calmodulin binding Source: UniProtKB-KW
- cAMP-dependent protein kinase regulator activity Source: RGD
- flavin adenine dinucleotide binding Source: RGD
- FMN binding Source: RGD
- heme binding Source: RGD
- Hsp90 protein binding Source: RGD
- identical protein binding Source: RGD
- metal ion binding Source: UniProtKB-KW
- NADP binding Source: InterPro
- NADPH-hemoprotein reductase activity Source: GO_Central
- nitric-oxide synthase activity Source: RGD
- nitric-oxide synthase binding Source: RGD
- oxidoreductase activity Source: GO_Central
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: GO_Central
- protein homodimerization activity Source: RGD
- protein kinase binding Source: RGD
- tetrahydrobiopterin binding Source: RGD
GO - Biological processi
- aging Source: RGD
- arginine catabolic process Source: RGD
- blood vessel remodeling Source: RGD
- cellular response to cytokine stimulus Source: MGI
- cellular response to drug Source: RGD
- cellular response to interferon-gamma Source: RGD
- cellular response to interleukin-1 Source: RGD
- cellular response to lipopolysaccharide Source: RGD
- cellular response to lithium ion Source: RGD
- cellular response to organic cyclic compound Source: MGI
- cellular response to redox state Source: RGD
- cellular response to tumor necrosis factor Source: RGD
- cellular response to vitamin D Source: RGD
- cGMP-mediated signaling Source: RGD
- circadian rhythm Source: RGD
- defense response to bacterium Source: UniProtKB
- defense response to Gram-negative bacterium Source: RGD
- endothelial cell proliferation Source: RGD
- glycoside metabolic process Source: RGD
- G protein-coupled receptor signaling pathway coupled to cGMP nucleotide second messenger Source: RGD
- inflammatory response Source: GO_Central
- interleukin-6 production Source: UniProtKB
- interleukin-8 production Source: UniProtKB
- intracellular signal transduction Source: RGD
- negative regulation of blood pressure Source: GO_Central
- negative regulation of gene expression Source: RGD
- negative regulation of protein catabolic process Source: RGD
- nitric oxide biosynthetic process Source: RGD
- nitric oxide mediated signal transduction Source: GO_Central
- peptidyl-cysteine S-nitrosylation Source: UniProtKB
- positive regulation of apoptotic process Source: RGD
- positive regulation of guanylate cyclase activity Source: GO_Central
- positive regulation of killing of cells of other organism Source: RGD
- prostaglandin secretion Source: UniProtKB
- regulation of blood pressure Source: RGD
- regulation of cell population proliferation Source: RGD
- regulation of cytokine production involved in inflammatory response Source: UniProtKB
- regulation of heart contraction Source: RGD
- regulation of insulin secretion Source: RGD
- response to activity Source: RGD
- response to bacterium Source: RGD
- response to dexamethasone Source: RGD
- response to estradiol Source: RGD
- response to hormone Source: RGD
- response to hypoxia Source: RGD
- response to immobilization stress Source: RGD
- response to lipopolysaccharide Source: RGD
- response to mechanical stimulus Source: RGD
- response to tumor necrosis factor Source: RGD
- retinoic acid receptor signaling pathway Source: RGD
- signal transduction Source: RGD
- superoxide metabolic process Source: UniProtKB
- wound healing Source: RGD
Keywordsi
Molecular function | Calmodulin-binding, Oxidoreductase |
Ligand | FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP, Zinc |
Enzyme and pathway databases
BRENDAi | 1.14.13.39, 5301 |
Reactomei | R-RNO-1222556, ROS and RNS production in phagocytes R-RNO-392154, Nitric oxide stimulates guanylate cyclase R-RNO-9033241, Peroxisomal protein import |
Names & Taxonomyi
Protein namesi | Recommended name: Nitric oxide synthase, inducible (EC:1.14.13.39)Alternative name(s): Inducible NO synthase Short name: Inducible NOS Short name: iNOS NOS type II Peptidyl-cysteine S-nitrosylase NOS2 |
Gene namesi | Name:Nos2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3185, Nos2 |
Subcellular locationi
Cytosol
- cytosol By similarity
Note: Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization.By similarity
Cytoskeleton
- cortical cytoskeleton Source: RGD
Cytosol
- cytosol Source: UniProtKB
Extracellular region or secreted
- extracellular space Source: RGD
Nucleus
- nucleus Source: BHF-UCL
Peroxisome
- peroxisome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB
- perinuclear region of cytoplasm Source: RGD
- vesicle membrane Source: RGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000170937 | 1 – 1147 | Nitric oxide synthase, inducibleAdd BLAST | 1147 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 564 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 572 | PhosphotyrosineCombined sources | 1 |
Post-translational modificationi
Polyubiquitinated; mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation.By similarity
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q06518 |
PRIDEi | Q06518 |
PTM databases
iPTMneti | Q06518 |
PhosphoSitePlusi | Q06518 |
Expressioni
Tissue specificityi
In normal kidney, expressed primarily in the medullary thick ascending limb, with minor amounts in the medullary collecting duct and vasa recta bundle.
Inductioni
By interferon gamma and lipopolysaccharides (LPS).
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with SLC9A3R1 (By similarity).
Interacts with GAPDH (By similarity).
Interacts with S100A8 and S100A9 to form the iNOS-S100A8/9 transnitrosylase complex (By similarity).
Interacts with SPSB1, SPSB2 and SPSB4 (By similarity).
Interacts with ELOC and CUL5 in the presence of SPSB1 or SPSB2 or SPSB4 (By similarity).
By similarityBinary interactionsi
Q06518
With | #Exp. | IntAct |
---|---|---|
Rela [Q7TQN4] | 3 | EBI-15919967,EBI-1187180 |
GO - Molecular functioni
- actin binding Source: RGD
- beta-catenin binding Source: RGD
- cadherin binding Source: RGD
- calmodulin binding Source: UniProtKB-KW
- Hsp90 protein binding Source: RGD
- identical protein binding Source: RGD
- nitric-oxide synthase binding Source: RGD
- protein homodimerization activity Source: RGD
- protein kinase binding Source: RGD
Protein-protein interaction databases
CORUMi | Q06518 |
DIPi | DIP-59942N |
IntActi | Q06518, 1 interactor |
STRINGi | 10116.ENSRNOP00000067662 |
Chemistry databases
BindingDBi | Q06518 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 536 – 674 | Flavodoxin-likePROSITE-ProRule annotationAdd BLAST | 139 | |
Domaini | 727 – 967 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 241 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 506 – 526 | Calmodulin-bindingSequence analysisAdd BLAST | 21 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 23 – 27 | DINNN-motif; mediates interaction with SPSB1, SPSB2 and SPSB4By similarity | 5 |
Sequence similaritiesi
Belongs to the NOS family.Curated
Phylogenomic databases
eggNOGi | KOG1158, Eukaryota |
InParanoidi | Q06518 |
OrthoDBi | 90349at2759 |
PhylomeDBi | Q06518 |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR012144, NOS_euk IPR004030, NOS_N IPR036119, NOS_N_sf IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit PF02898, NO_synthase, 1 hit |
PIRSFi | PIRSF000333, NOS, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SUPFAMi | SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF56512, SSF56512, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit PS60001, NOS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q06518-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MACPWKFLFR VKSYQGDLKE EKDINNNVEK TPGAIPSPTT QDDPKSHKHQ
60 70 80 90 100
NGFPQFLTGT AQNVPESLDK LHVTPSTRPQ HVRIKNWGNG EIFHDTLHHK
110 120 130 140 150
ATSDISCKSK LCMGSIMNSK SLTRGPRDKP TPVEELLPQA IEFINQYYGS
160 170 180 190 200
FKEAKIEEHL ARLEAVTKEI ETTGTYQLTL DELIFATKMA WRNAPRCIGR
210 220 230 240 250
IQWSNLQVFD ARSCSTASEM FQHICRHILY ATNSGNIRSA ITVFPQRSDG
260 270 280 290 300
KHDFRIWNSQ LIRYAGYQMP DGTIRGDPAT LEFTQLCIDL GWKPRYGRFD
310 320 330 340 350
VLPLVLQAHG QDPEVFEIPP DLVLEVTMEH PKYEWFQELG LKWYALPAVA
360 370 380 390 400
NMLLEVGGLE FPACPFNGWY MGTEIGVRDF CDTQRYNILE EVGRRMGLET
410 420 430 440 450
HTLASLWKDR AVTEINAAVL HSFQKQNVTI MDHHTASESF MKHMQNEYRA
460 470 480 490 500
RGGCPADWIW LVPPVSGSIT PVFHQEMLNY VLSPFYYYQI EPWKTHIWQD
510 520 530 540 550
EKLRPRRREI RFTVLVKAVF FASVLMRKVM ASRVRATVLF ATETGKSEAL
560 570 580 590 600
ARDLAALFSY AFNTKVVCME QYKANTLEEE QLLLVVTSTF GNGDCPSNGQ
610 620 630 640 650
TLKKSLFMMK ELGHTFRYAV FGLGSSMYPQ FCAFAHDIDQ KLSHLGASQL
660 670 680 690 700
APTGEGDELS GQEDAFRSWA VQTFRAACET FDVRSKHCIQ IPKRYTSNAT
710 720 730 740 750
WEPEQYKLTQ SPESLDLNKA LSSIHAKNVF TMRLKSLQNL QSEKSSRTTL
760 770 780 790 800
LVQLTFEGSR GPSYLPGEHL GIFPGNQTAL VQGILERVVD CSSPDQTVCL
810 820 830 840 850
EVLDESGSYW VKDKRLPPCS LRQALTYFLD ITTPPTQLQL HKLARFATEE
860 870 880 890 900
THRQRLEALC QPSEYNDWKF SNNPTFLEVL EEFPSLRVPA AFLLSQLPIL
910 920 930 940 950
KPRYYSISSS QDHTPSEVHL TVAVVTYRTR DGQGPLHHGV CSTWINNLKP
960 970 980 990 1000
EDPVPCFVRS VSGFQLPEDP SQPCILIGPG TGIAPFRSFW QQRLHDSQHR
1010 1020 1030 1040 1050
GLKGGRMTLV FGCRHPEEDH LYQEEMQEMV RKGVLFQVHT GYSRLPGKPK
1060 1070 1080 1090 1100
VYVQDILQKE LADEVFSVLH GEQGHLYVCG DVRMARDVAT TLKKLVAAKL
1110 1120 1130 1140
NLSEEQVEDY FFQLKSQKRY HEDIFGAVFS YGAKKGNTLE EPKGTRL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM0RDG2 | M0RDG2_RAT | Nitric oxide synthase | Nos2 | 1,147 | Annotation score: | ||
F1LSH9 | F1LSH9_RAT | Nitric oxide synthase | LOC497963 Nos2 | 1,111 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 10 | R → K in BAA07994 (Ref. 7) Curated | 1 | |
Sequence conflicti | 72 | H → Y in BAA03138 (PubMed:7680561).Curated | 1 | |
Sequence conflicti | 107 | C → R in AAC13747 (PubMed:7513765).Curated | 1 | |
Sequence conflicti | 128 | D → V in BAA12035 (PubMed:8913516).Curated | 1 | |
Sequence conflicti | 130 | P → H in AAC13747 (PubMed:7513765).Curated | 1 | |
Sequence conflicti | 171 | E → G in BAA12035 (PubMed:8913516).Curated | 1 | |
Sequence conflicti | 195 | P → S in BAA12035 (PubMed:8913516).Curated | 1 | |
Sequence conflicti | 248 | S → N in AAC83553 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 248 | S → T (PubMed:7513765).Curated | 1 | |
Sequence conflicti | 248 | S → T (PubMed:7682072).Curated | 1 | |
Sequence conflicti | 264 | Y → I in AAC13747 (PubMed:7513765).Curated | 1 | |
Sequence conflicti | 271 | D → A in AAC83554 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 277 | D → E in AAC13747 (PubMed:7513765).Curated | 1 | |
Sequence conflicti | 348 | A → P in BAA03138 (PubMed:7680561).Curated | 1 | |
Sequence conflicti | 349 | V → A in CAA54208 (PubMed:7519448).Curated | 1 | |
Sequence conflicti | 380 | F → L (PubMed:7540573).Curated | 1 | |
Sequence conflicti | 380 | F → L (Ref. 7) Curated | 1 | |
Sequence conflicti | 380 | F → L (PubMed:8913516).Curated | 1 | |
Sequence conflicti | 380 | F → L in AAC83553 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 380 | F → L in AAC83554 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 395 | R → S in BAA02090 (PubMed:7693462).Curated | 1 | |
Sequence conflicti | 399 | E → G in AAC83553 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 412 | V → A in AAC13747 (PubMed:7513765).Curated | 1 | |
Sequence conflicti | 477 | M → I (Ref. 13) Curated | 1 | |
Sequence conflicti | 513 | T → R in AAB18620 (Ref. 11) Curated | 1 | |
Sequence conflicti | 515 | L → W in AAB31028 (PubMed:7516453).Curated | 1 | |
Sequence conflicti | 545 | G → R in AAB31028 (PubMed:7516453).Curated | 1 | |
Sequence conflicti | 551 | A → R in AAB18620 (Ref. 11) Curated | 1 | |
Sequence conflicti | 556 | A → S in AAB31028 (PubMed:7516453).Curated | 1 | |
Sequence conflicti | 559 | S → T in AAC83553 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 559 | S → T in AAC83554 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 564 | T → N in AAB31028 (PubMed:7516453).Curated | 1 | |
Sequence conflicti | 570 | E → D in AAB31028 (PubMed:7516453).Curated | 1 | |
Sequence conflicti | 583 | L → P (PubMed:7682072).Curated | 1 | |
Sequence conflicti | 583 | L → P (PubMed:9535415).Curated | 1 | |
Sequence conflicti | 591 | G → A in AAB31028 (PubMed:7516453).Curated | 1 | |
Sequence conflicti | 591 | G → V (PubMed:7680561).Curated | 1 | |
Sequence conflicti | 591 | G → V (PubMed:7519448).Curated | 1 | |
Sequence conflicti | 619 | A → R in AAA85861 (PubMed:7540573).Curated | 1 | |
Sequence conflicti | 640 | Q → P in AAC83553 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 664 | D → G in AAB18620 (Ref. 11) Curated | 1 | |
Sequence conflicti | 679 – 680 | ET → VP in BAA03138 (PubMed:7680561).Curated | 2 | |
Sequence conflicti | 690 | Q → P in AAB18620 (Ref. 11) Curated | 1 | |
Sequence conflicti | 711 | S → N in AAB18620 (Ref. 11) Curated | 1 | |
Sequence conflicti | 714 – 715 | SL → TR in AAB18620 (Ref. 11) Curated | 2 | |
Sequence conflicti | 714 | S → P (PubMed:7540573).Curated | 1 | |
Sequence conflicti | 714 | S → P (Ref. 7) Curated | 1 | |
Sequence conflicti | 714 | S → P (PubMed:8913516).Curated | 1 | |
Sequence conflicti | 714 | S → P in AAC83553 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 714 | S → P in AAC83554 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 714 | S → P in AAC16401 (PubMed:9535415).Curated | 1 | |
Sequence conflicti | 719 | K → R in AAB18620 (Ref. 11) Curated | 1 | |
Sequence conflicti | 721 | L → P (PubMed:7519448).Curated | 1 | |
Sequence conflicti | 722 | S → R (PubMed:7680561).Curated | 1 | |
Sequence conflicti | 722 | S → R (PubMed:7519448).Curated | 1 | |
Sequence conflicti | 722 | S → R (Ref. 11) Curated | 1 | |
Sequence conflicti | 724 – 726 | IHA → FLN (Ref. 11) Curated | 3 | |
Sequence conflicti | 730 | F → I in AAB18620 (Ref. 11) Curated | 1 | |
Sequence conflicti | 731 | T → A in AAC83553 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 740 | L → P in CAA54208 (PubMed:7519448).Curated | 1 | |
Sequence conflicti | 779 | A → G in AAC13747 (PubMed:7513765).Curated | 1 | |
Sequence conflicti | 834 | P → S in BAA12035 (PubMed:8913516).Curated | 1 | |
Sequence conflicti | 844 | A → G in BAA03138 (PubMed:7680561).Curated | 1 | |
Sequence conflicti | 895 | S → L in BAA02090 (PubMed:7693462).Curated | 1 | |
Sequence conflicti | 911 | Q → L in AAC13747 (PubMed:7513765).Curated | 1 | |
Sequence conflicti | 925 | V → D in BAA12035 (PubMed:8913516).Curated | 1 | |
Sequence conflicti | 937 | H → N in AAC83554 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 999 | H → R (PubMed:7540573).Curated | 1 | |
Sequence conflicti | 999 | H → R (Ref. 7) Curated | 1 | |
Sequence conflicti | 999 | H → R in AAC83553 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 999 | H → R in AAC83554 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 1008 – 1009 | TL → NF in AAC83554 (PubMed:9851365).Curated | 2 | |
Sequence conflicti | 1016 | P → R (PubMed:7682072).Curated | 1 | |
Sequence conflicti | 1016 | P → S (PubMed:8913516).Curated | 1 | |
Sequence conflicti | 1017 | E → R (PubMed:7682072).Curated | 1 | |
Sequence conflicti | 1017 | E → R (PubMed:8913516).Curated | 1 | |
Sequence conflicti | 1024 | E → K in AAC83554 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 1076 | L → I in AAC83553 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 1084 | M → I in CAA54208 (PubMed:7519448).Curated | 1 | |
Sequence conflicti | 1129 | F → V in AAC83554 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 1133 | A → V (PubMed:7540573).Curated | 1 | |
Sequence conflicti | 1133 | A → V (Ref. 7) Curated | 1 | |
Sequence conflicti | 1133 | A → V in AAC83553 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 1133 | A → V in AAC83554 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 1138 | T → A (PubMed:7540573).Curated | 1 | |
Sequence conflicti | 1138 | T → A (Ref. 7) Curated | 1 | |
Sequence conflicti | 1138 | T → A in AAC83553 (PubMed:9851365).Curated | 1 | |
Sequence conflicti | 1138 | T → A in AAC83554 (PubMed:9851365).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D14051 mRNA Translation: BAA03138.1 U26686 mRNA Translation: AAA85861.1 U03699 mRNA Translation: AAC13747.1 D12520 mRNA Translation: BAA02090.1 L12562 mRNA Translation: AAA41720.1 X76881 mRNA Translation: CAA54208.1 D44591 mRNA Translation: BAA07994.1 D83661 mRNA Translation: BAA12035.1 AF049656 mRNA Translation: AAC83553.1 AF051164 mRNA Translation: AAC83554.1 AF006619 mRNA Translation: AAC16401.1 AF006620 mRNA Translation: AAC16402.1 U48829 mRNA Translation: AAB18620.1 S71597 mRNA Translation: AAB31028.2 L36063 mRNA Translation: AAC02242.1 |
PIRi | I53165 I56575 JC5027 S38253 S47647 |
RefSeqi | NP_036743.3, NM_012611.3 |
Genome annotation databases
GeneIDi | 24599 |
KEGGi | rno:24599 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D14051 mRNA Translation: BAA03138.1 U26686 mRNA Translation: AAA85861.1 U03699 mRNA Translation: AAC13747.1 D12520 mRNA Translation: BAA02090.1 L12562 mRNA Translation: AAA41720.1 X76881 mRNA Translation: CAA54208.1 D44591 mRNA Translation: BAA07994.1 D83661 mRNA Translation: BAA12035.1 AF049656 mRNA Translation: AAC83553.1 AF051164 mRNA Translation: AAC83554.1 AF006619 mRNA Translation: AAC16401.1 AF006620 mRNA Translation: AAC16402.1 U48829 mRNA Translation: AAB18620.1 S71597 mRNA Translation: AAB31028.2 L36063 mRNA Translation: AAC02242.1 |
PIRi | I53165 I56575 JC5027 S38253 S47647 |
RefSeqi | NP_036743.3, NM_012611.3 |
3D structure databases
SMRi | Q06518 |
ModBasei | Search... |
Protein-protein interaction databases
CORUMi | Q06518 |
DIPi | DIP-59942N |
IntActi | Q06518, 1 interactor |
STRINGi | 10116.ENSRNOP00000067662 |
Chemistry databases
BindingDBi | Q06518 |
ChEMBLi | CHEMBL3051 |
PTM databases
iPTMneti | Q06518 |
PhosphoSitePlusi | Q06518 |
Proteomic databases
PaxDbi | Q06518 |
PRIDEi | Q06518 |
Genome annotation databases
GeneIDi | 24599 |
KEGGi | rno:24599 |
Organism-specific databases
CTDi | 4843 |
RGDi | 3185, Nos2 |
Phylogenomic databases
eggNOGi | KOG1158, Eukaryota |
InParanoidi | Q06518 |
OrthoDBi | 90349at2759 |
PhylomeDBi | Q06518 |
Enzyme and pathway databases
BRENDAi | 1.14.13.39, 5301 |
Reactomei | R-RNO-1222556, ROS and RNS production in phagocytes R-RNO-392154, Nitric oxide stimulates guanylate cyclase R-RNO-9033241, Peroxisomal protein import |
Miscellaneous databases
PROi | PR:Q06518 |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR012144, NOS_euk IPR004030, NOS_N IPR036119, NOS_N_sf IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit PF02898, NO_synthase, 1 hit |
PIRSFi | PIRSF000333, NOS, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SUPFAMi | SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF56512, SSF56512, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit PS60001, NOS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NOS2_RAT | |
Accessioni | Q06518Primary (citable) accession number: Q06518 Secondary accession number(s): O35765 Q64558 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 200 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families