UniProtKB - Q06507 (ATF4_MOUSE)
Cyclic AMP-dependent transcription factor ATF-4
Atf4
Functioni
GO - Molecular functioni
- cAMP response element binding protein binding Source: UniProtKB
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- leucine zipper domain binding Source: MGI
- protein C-terminus binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- protein kinase binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- RNA polymerase II transcription factor binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription factor binding Source: MGI
- transcription regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- bone mineralization Source: UniProtKB
- cellular calcium ion homeostasis Source: MGI
- cellular response to amino acid starvation Source: UniProtKB
- cellular response to dopamine Source: MGI
- cellular response to glucose starvation Source: MGI
- cellular response to oxidative stress Source: UniProtKB
- cellular response to oxygen-glucose deprivation Source: Ensembl
- circadian regulation of gene expression Source: UniProtKB
- circadian rhythm Source: UniProtKB
- embryonic hemopoiesis Source: UniProtKB
- endoplasmic reticulum unfolded protein response Source: UniProtKB
- gamma-aminobutyric acid signaling pathway Source: MGI
- gluconeogenesis Source: MGI
- HRI-mediated signaling Source: UniProtKB
- integrated stress response signaling Source: UniProtKB
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
- L-asparagine metabolic process Source: MGI
- lens fiber cell morphogenesis Source: UniProtKB
- mRNA transcription by RNA polymerase II Source: UniProtKB
- negative regulation of cold-induced thermogenesis Source: YuBioLab
- negative regulation of oxidative stress-induced neuron death Source: MGI
- negative regulation of potassium ion transport Source: MGI
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- neuron differentiation Source: Ensembl
- PERK-mediated unfolded protein response Source: UniProtKB
- positive regulation of apoptotic process Source: GO_Central
- positive regulation of biomineral tissue development Source: MGI
- positive regulation of gene expression Source: ParkinsonsUK-UCL
- positive regulation of neuron apoptotic process Source: UniProtKB
- positive regulation of sodium-dependent phosphate transport Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase I Source: MGI
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
- positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: MGI
- positive regulation of vascular associated smooth muscle cell apoptotic process Source: MGI
- positive regulation of vascular endothelial growth factor production Source: MGI
- regulation of osteoblast differentiation Source: UniProtKB
- regulation of synaptic plasticity Source: UniProtKB
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- response to endoplasmic reticulum stress Source: UniProtKB
- response to manganese-induced endoplasmic reticulum stress Source: Ensembl
- response to nutrient levels Source: MGI
- response to toxic substance Source: Ensembl
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Biological rhythms, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Cyclic AMP-dependent transcription factor ATF-4CuratedShort name: cAMP-dependent transcription factor ATF-4Curated Alternative name(s): Activating transcription factor 41 Publication C/EBP-related ATF1 Publication Short name: C/ATF1 Publication Cyclic AMP-responsive element-binding protein 21 Publication Short name: CREB-21 Publication Short name: cAMP-responsive element-binding protein 21 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88096, Atf4 |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
- Nucleus speckle By similarity
Plasma membrane
- Cell membrane By similarity
Cytoskeleton
- centrosome By similarity
Other locations
- Cytoplasm 1 Publication
Note: Colocalizes with GABBR1 in hippocampal neuron dendritic membranes. Colocalizes with NEK6 in the centrosome (By similarity). Recruited to nuclear speckles following interaction with EP300/p300 (By similarity).By similarity
Cytoskeleton
- microtubule organizing center Source: UniProtKB-SubCell
Nucleus
- ATF1-ATF4 transcription factor complex Source: ParkinsonsUK-UCL
- ATF4-CREB1 transcription factor complex Source: ParkinsonsUK-UCL
- nuclear periphery Source: MGI
- nuclear speck Source: UniProtKB-SubCell
- nucleus Source: UniProtKB
Plasma Membrane
- dendrite membrane Source: MGI
Other locations
- CHOP-ATF4 complex Source: MGI
- cytoplasm Source: UniProtKB
- Lewy body core Source: MGI
- neuron projection Source: MGI
- protein-containing complex Source: MGI
- RNA polymerase II transcription regulator complex Source: MGI
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 212 | T → A: Promotes stabilization due to impaired ubiquitination; when associated with A-223; A-230; A-234 and A-247. 1 Publication | 1 | |
Mutagenesisi | 218 | S → A: Promotes stabilization due to impaired ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 223 | S → A: Promotes stabilization due to impaired ubiquitination; when associated with A-212; A-230; A-234 and A-247. 1 Publication | 1 | |
Mutagenesisi | 230 | S → A: Promotes stabilization due to impaired ubiquitination; when associated with A-212; A-223; A-234 and A-247. 1 Publication | 1 | |
Mutagenesisi | 234 | S → A: Promotes stabilization due to impaired ubiquitination; when associated with A-212; A-223; A-230 and A-247. 1 Publication | 1 | |
Mutagenesisi | 247 | S → A: Promotes stabilization due to impaired ubiquitination; when associated with A-212; A-223; A-230 and A-234. Does not affect phosphorylation by RPS6KA3/RSK2. 2 Publications | 1 | |
Mutagenesisi | 251 | S → A, R or D: Abolished phosphorylation by RPS6KA3/RSK2. 1 Publication | 1 | |
Mutagenesisi | 254 | S → A: Does not affect phosphorylation by RPS6KA3/RSK2. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076585 | 1 – 349 | Cyclic AMP-dependent transcription factor ATF-4Add BLAST | 349 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 60 | 4-hydroxyproline1 Publication | 1 | |
Modified residuei | 212 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 214 | PhosphoserineBy similarity | 1 | |
Modified residuei | 218 | Phosphoserine1 Publication | 1 | |
Modified residuei | 223 | Phosphoserine1 Publication | 1 | |
Modified residuei | 230 | Phosphoserine1 Publication | 1 | |
Modified residuei | 234 | Phosphoserine1 Publication | 1 | |
Modified residuei | 235 | 4-hydroxyproline1 Publication | 1 | |
Modified residuei | 247 | Phosphoserine1 Publication | 1 | |
Modified residuei | 251 | Phosphoserine; by RPS6KA31 Publication | 1 | |
Cross-linki | 258 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 270 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 309 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Hydroxylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | Q06507 |
PRIDEi | Q06507 |
PTM databases
iPTMneti | Q06507 |
PhosphoSitePlusi | Q06507 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000042406, Expressed in retina and 348 other tissues |
ExpressionAtlasi | Q06507, baseline and differential |
Genevisiblei | Q06507, MM |
Interactioni
Subunit structurei
Binds DNA as a homodimer and as a heterodimer (PubMed:23624402). Heterodimer; heterodimerizes with CEBPB (PubMed:11018027). Heterodimer; heterodimerizes with DDIT3/CHOP (PubMed:23624402).
Interacts with CEP290 (via an N-terminal region) (By similarity).
Interacts with NEK6, DAPK2 (isoform 2) and ZIPK/DAPK3 (By similarity).
Interacts (via its leucine zipper domain) with GABBR1 and GABBR2 (via their C-termini) (By similarity).
Forms a heterodimer with TXLNG in osteoblasts (PubMed:15911876).
Interacts (via its DNA binding domain) with FOXO1 (C-terminal half); the interaction occurs in osteoblasts and regulates glucose homeostasis through suppression of beta-cell proliferation and a decrease in insulin production (PubMed:22298775).
Interacts with SATB2; the interaction results in enhanced DNA binding and transactivation by these transcription factors (PubMed:16751105).
Interacts with ABRAXAS2 (PubMed:22974638).
Interacts with TRIB3, inhibiting the transactivation activity of ATF4 (PubMed:17369260).
Interacts with DISC1; which inhibits ATF4 transcription factor activity by disrupting ATF4 dimerization and DNA-binding (PubMed:31444471).
Interacts with EP300/p300; EP300/p300 stabilizes ATF4 and increases its transcriptional activity independently of its catalytic activity by preventing its ubiquitination (By similarity).
By similarity8 PublicationsBinary interactionsi
Hide detailsQ06507
With | #Exp. | IntAct |
---|---|---|
Dapk3 [O54784] | 3 | EBI-77383,EBI-77359 |
Ddit3 [P35639] | 3 | EBI-77383,EBI-10636142 |
Trib3 [Q8K4K2] | 3 | EBI-77383,EBI-448962 |
GO - Molecular functioni
- cAMP response element binding protein binding Source: UniProtKB
- leucine zipper domain binding Source: MGI
- protein C-terminus binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- protein kinase binding Source: MGI
- RNA polymerase II transcription factor binding Source: MGI
- transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 198235, 11 interactors |
ComplexPortali | CPX-6, ATF4-CREB1 transcription factor complex CPX-7, ATF1-ATF4 transcription factor complex |
CORUMi | Q06507 |
DIPi | DIP-30969N |
IntActi | Q06507, 9 interactors |
STRINGi | 10090.ENSMUSP00000105234 |
Miscellaneous databases
RNActi | Q06507, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q06507 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 276 – 339 | bZIPPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 278 – 298 | Basic motifPROSITE-ProRule annotationAdd BLAST | 21 | |
Regioni | 303 – 339 | Interaction with GABBR1By similarityAdd BLAST | 37 | |
Regioni | 304 – 332 | Leucine-zipperPROSITE-ProRule annotationAdd BLAST | 29 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 214 – 223 | BetaTrCP degron motif1 Publication | 10 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4571, Eukaryota |
GeneTreei | ENSGT00530000063801 |
HOGENOMi | CLU_055748_1_0_1 |
InParanoidi | Q06507 |
OMAi | IVNPIGH |
OrthoDBi | 1524842at2759 |
TreeFami | TF316136 |
Family and domain databases
InterProi | View protein in InterPro IPR029811, ATF4 IPR004827, bZIP |
PANTHERi | PTHR13044:SF32, PTHR13044:SF32, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MTEMSFLNSE VLAGDLMSPF DQSGLGAEES LGLLDDYLEV AKHLKPHGFS
60 70 80 90 100
SDKAGSSEWP AMDDGLASAS DTGKEDAFSG TDWMLEKMDL KEFDFDALFR
110 120 130 140 150
MDDLETMPDE LLTTLDDTCD LFAPLVQETN KEPPQTVNPI GHLPESLIKV
160 170 180 190 200
DQVAPFTFLQ PFPCSPGVLS STPEHSFSLE LGSEVDISEG DRKPDSAAYI
210 220 230 240 250
TLIPPCVKEE DTPSDNDSGI CMSPESYLGS PQHSPSTSRA PPDNLPSPGG
260 270 280 290 300
SRGSPRPKPY DPPGVSLTAK VKTEKLDKKL KKMEQNKTAA TRYRQKKRAE
310 320 330 340
QEALTGECKE LEKKNEALKE KADSLAKEIQ YLKDLIEEVR KARGKKRVP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A2R8VI82 | A0A2R8VI82_MOUSE | Cyclic AMP-dependent transcription ... | Atf4 | 116 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 345 | K → Q in AAA40476 (PubMed:8506317).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13791 mRNA Translation: AAA40476.1 M94087 mRNA Translation: AAA53043.1 Different initiation. AK138657 mRNA Translation: BAE23736.1 AK144777 mRNA Translation: BAE26060.1 AK146193 mRNA Translation: BAE26967.1 AK156298 mRNA Translation: BAE33662.1 CH466550 Genomic DNA Translation: EDL04604.1 CH466550 Genomic DNA Translation: EDL04605.1 BC085169 mRNA Translation: AAH85169.1 |
CCDSi | CCDS37145.1 |
RefSeqi | NP_001274109.1, NM_001287180.1 NP_033846.2, NM_009716.3 |
Genome annotation databases
Ensembli | ENSMUST00000109605; ENSMUSP00000105234; ENSMUSG00000042406 |
GeneIDi | 11911 |
KEGGi | mmu:11911 |
UCSCi | uc007wvl.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13791 mRNA Translation: AAA40476.1 M94087 mRNA Translation: AAA53043.1 Different initiation. AK138657 mRNA Translation: BAE23736.1 AK144777 mRNA Translation: BAE26060.1 AK146193 mRNA Translation: BAE26967.1 AK156298 mRNA Translation: BAE33662.1 CH466550 Genomic DNA Translation: EDL04604.1 CH466550 Genomic DNA Translation: EDL04605.1 BC085169 mRNA Translation: AAH85169.1 |
CCDSi | CCDS37145.1 |
RefSeqi | NP_001274109.1, NM_001287180.1 NP_033846.2, NM_009716.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6IRR | NMR | - | A | 314-349 | [»] | |
SMRi | Q06507 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198235, 11 interactors |
ComplexPortali | CPX-6, ATF4-CREB1 transcription factor complex CPX-7, ATF1-ATF4 transcription factor complex |
CORUMi | Q06507 |
DIPi | DIP-30969N |
IntActi | Q06507, 9 interactors |
STRINGi | 10090.ENSMUSP00000105234 |
PTM databases
iPTMneti | Q06507 |
PhosphoSitePlusi | Q06507 |
Proteomic databases
PaxDbi | Q06507 |
PRIDEi | Q06507 |
Protocols and materials databases
Antibodypediai | 12687, 1054 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000109605; ENSMUSP00000105234; ENSMUSG00000042406 |
GeneIDi | 11911 |
KEGGi | mmu:11911 |
UCSCi | uc007wvl.2, mouse |
Organism-specific databases
CTDi | 468 |
MGIi | MGI:88096, Atf4 |
Phylogenomic databases
eggNOGi | KOG4571, Eukaryota |
GeneTreei | ENSGT00530000063801 |
HOGENOMi | CLU_055748_1_0_1 |
InParanoidi | Q06507 |
OMAi | IVNPIGH |
OrthoDBi | 1524842at2759 |
TreeFami | TF316136 |
Miscellaneous databases
BioGRID-ORCSi | 11911, 10 hits in 21 CRISPR screens |
ChiTaRSi | Atf4, mouse |
PROi | PR:Q06507 |
RNActi | Q06507, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000042406, Expressed in retina and 348 other tissues |
ExpressionAtlasi | Q06507, baseline and differential |
Genevisiblei | Q06507, MM |
Family and domain databases
InterProi | View protein in InterPro IPR029811, ATF4 IPR004827, bZIP |
PANTHERi | PTHR13044:SF32, PTHR13044:SF32, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ATF4_MOUSE | |
Accessioni | Q06507Primary (citable) accession number: Q06507 Secondary accession number(s): Q5U4B2, Q61906 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 161 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families