UniProtKB - Q06505 (IWS1_YEAST)
Protein
Transcription factor SPN1
Gene
SPN1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Transcription factor involved in RNA polymerase II transcription regulation. May function in both SPT15/TBP post-recruitment and recruitment steps of transcription.2 Publications
Miscellaneous
Present with 2830 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- RNA polymerase II complex binding Source: SGD
- RNA polymerase II transcription factor binding Source: SGD
GO - Biological processi
- chromatin maintenance Source: SGD
- poly(A)+ mRNA export from nucleus Source: SGD
- regulation of nucleosome density Source: SGD
- regulation of transcription by RNA polymerase II Source: SGD
- regulation of transcription elongation from RNA polymerase II promoter Source: SGD
Keywordsi
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-75955, RNA Polymerase II Transcription Elongation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor SPN1Alternative name(s): Interacts with SPT6 protein 1 Suppresses postrecruitment functions protein 1 |
Gene namesi | Name:SPN1 Synonyms:IWS1 Ordered Locus Names:YPR133C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006337, SPN1 |
VEuPathDBi | FungiDB:YPR133C |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Nucleus
- nucleus Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 192 | K → N: Suppresses postrecruitment-defective SPT15/TBP alleles. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083366 | 1 – 410 | Transcription factor SPN1Add BLAST | 410 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 15 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 23 | Phosphoserine; by ATM or ATRCombined sources | 1 | |
Modified residuei | 40 | PhosphoserineCombined sources | 1 | |
Modified residuei | 85 | PhosphoserineCombined sources | 1 | |
Modified residuei | 86 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 89 | PhosphoserineCombined sources1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q06505 |
PaxDbi | Q06505 |
PRIDEi | Q06505 |
PTM databases
iPTMneti | Q06505 |
Interactioni
Subunit structurei
Interacts with ABD1, RBP1, SPT5 and SPT6.
2 PublicationsBinary interactionsi
Q06505
With | #Exp. | IntAct |
---|---|---|
SPT6 [P23615] | 7 | EBI-32596,EBI-17947 |
GO - Molecular functioni
- RNA polymerase II complex binding Source: SGD
- RNA polymerase II transcription factor binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 36299, 441 interactors |
DIPi | DIP-3823N |
IntActi | Q06505, 12 interactors |
MINTi | Q06505 |
STRINGi | 4932.YPR133C |
Miscellaneous databases
RNActi | Q06505, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q06505 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q06505 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 219 – 296 | TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST | 78 |
Sequence similaritiesi
Belongs to the IWS1 family.Curated
Phylogenomic databases
eggNOGi | KOG1793, Eukaryota |
GeneTreei | ENSGT00940000169144 |
HOGENOMi | CLU_045275_0_0_1 |
InParanoidi | Q06505 |
OMAi | ERMQDDK |
Family and domain databases
Gene3Di | 1.20.930.10, 1 hit |
IDEALi | IID50050 |
InterProi | View protein in InterPro IPR035441, TFIIS/LEDGF_dom_sf IPR017923, TFIIS_N |
Pfami | View protein in Pfam PF08711, Med26, 1 hit |
PROSITEi | View protein in PROSITE PS51319, TFIIS_N, 1 hit |
i Sequence
Sequence statusi: Complete.
Q06505-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTADQEQPK VVEATPEDGT ASSQKSTINA ENENTKQNQS MEPQETSKGT
60 70 80 90 100
SNDTKDPDNG EKNEEAAIDE NSNVEAAERK RKHISTDFSD DDLEKEEHND
110 120 130 140 150
QSLQPTVENR ASKDRDSSAT PSSRQELEEK LDRILKKPKV RRTRRDEDDL
160 170 180 190 200
EQYLDEKILR LKDEMNIAAQ LDIDTLNKRI ETGDTSLIAM QKVKLLPKVV
210 220 230 240 250
SVLSKANLAD TILDNNLLQS VRIWLEPLPD GSLPSFEIQK SLFAALNDLP
260 270 280 290 300
VKTEHLKESG LGRVVIFYTK SKRVEAQLAR LAEKLIAEWT RPIIGASDNY
310 320 330 340 350
RDKRIMQLEF DSEKLRKKSV MDSAKNRKKK SKSGEDPTSR GSSVQTLYEQ
360 370 380 390 400
AAARRNRAAA PAQTTTDYKY APVSNLSAVP TNARAVGVGS TLNNSEMYKR
410
LTSRLNKKHK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U40829 Genomic DNA Translation: AAB68274.1 AY558069 Genomic DNA Translation: AAS56395.1 BK006949 Genomic DNA Translation: DAA11545.1 |
PIRi | S69023 |
RefSeqi | NP_015458.1, NM_001184230.1 |
Genome annotation databases
EnsemblFungii | YPR133C_mRNA; YPR133C; YPR133C |
GeneIDi | 856251 |
KEGGi | sce:YPR133C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U40829 Genomic DNA Translation: AAB68274.1 AY558069 Genomic DNA Translation: AAS56395.1 BK006949 Genomic DNA Translation: DAA11545.1 |
PIRi | S69023 |
RefSeqi | NP_015458.1, NM_001184230.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3NFQ | X-ray | 1.85 | A/B | 141-310 | [»] | |
3O8Z | X-ray | 2.15 | A | 148-295 | [»] | |
3OAK | X-ray | 2.15 | A/B | 148-292 | [»] | |
SMRi | Q06505 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36299, 441 interactors |
DIPi | DIP-3823N |
IntActi | Q06505, 12 interactors |
MINTi | Q06505 |
STRINGi | 4932.YPR133C |
PTM databases
iPTMneti | Q06505 |
Proteomic databases
MaxQBi | Q06505 |
PaxDbi | Q06505 |
PRIDEi | Q06505 |
Genome annotation databases
EnsemblFungii | YPR133C_mRNA; YPR133C; YPR133C |
GeneIDi | 856251 |
KEGGi | sce:YPR133C |
Organism-specific databases
SGDi | S000006337, SPN1 |
VEuPathDBi | FungiDB:YPR133C |
Phylogenomic databases
eggNOGi | KOG1793, Eukaryota |
GeneTreei | ENSGT00940000169144 |
HOGENOMi | CLU_045275_0_0_1 |
InParanoidi | Q06505 |
OMAi | ERMQDDK |
Enzyme and pathway databases
Reactomei | R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-75955, RNA Polymerase II Transcription Elongation |
Miscellaneous databases
EvolutionaryTracei | Q06505 |
PROi | PR:Q06505 |
RNActi | Q06505, protein |
Family and domain databases
Gene3Di | 1.20.930.10, 1 hit |
IDEALi | IID50050 |
InterProi | View protein in InterPro IPR035441, TFIIS/LEDGF_dom_sf IPR017923, TFIIS_N |
Pfami | View protein in Pfam PF08711, Med26, 1 hit |
PROSITEi | View protein in PROSITE PS51319, TFIIS_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IWS1_YEAST | |
Accessioni | Q06505Primary (citable) accession number: Q06505 Secondary accession number(s): D6W4C9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | November 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families