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UniProtKB - Q06496 (NPT2A_RAT)
Protein
Sodium-dependent phosphate transport protein 2A
Gene
Slc34a1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Involved in actively transporting phosphate into cells via Na+ cotransport in the renal brush border membrane. Probably mediates 70-80% of the apical influx.
1 PublicationMiscellaneous
The cotransport has a Na+:Pi stoichiometry of 3:1 and is electrogenic.
PTH induces lysosomal degradation of the cotransporter.
GO - Molecular functioni
- identical protein binding Source: RGD
- PDZ domain binding Source: RGD
- protein-containing complex binding Source: RGD
- sodium:phosphate symporter activity Source: UniProtKB
GO - Biological processi
- arsenate ion transmembrane transport Source: RGD
- bone remodeling Source: RGD
- cellular phosphate ion homeostasis Source: GO_Central
- cellular response to metal ion Source: RGD
- cellular response to parathyroid hormone stimulus Source: RGD
- cellular response to staurosporine Source: RGD
- dentinogenesis Source: RGD
- gentamycin metabolic process Source: RGD
- glycoprotein metabolic process Source: RGD
- indole metabolic process Source: RGD
- kidney development Source: RGD
- ossification Source: RGD
- phosphate ion homeostasis Source: RGD
- phosphate ion transmembrane transport Source: RGD
- phosphate ion transport Source: RGD
- positive regulation of membrane potential Source: RGD
- positive regulation of phosphate transmembrane transport Source: RGD
- positive regulation of sodium-dependent phosphate transport Source: RGD
- response to cadmium ion Source: RGD
- response to estradiol Source: RGD
- response to growth hormone Source: RGD
- response to lead ion Source: RGD
- response to magnesium ion Source: RGD
- response to mercury ion Source: RGD
- response to nutrient Source: RGD
- response to parathyroid hormone Source: RGD
- response to peptide Source: RGD
- response to peptide hormone Source: RGD
- response to potassium ion Source: RGD
- response to thyroid hormone Source: RGD
- response to vitamin A Source: RGD
- response to xenobiotic stimulus Source: RGD
- sodium-dependent phosphate transport Source: RGD
- sodium ion import across plasma membrane Source: RGD
- tricarboxylic acid metabolic process Source: RGD
Keywordsi
Biological process | Ion transport, Sodium transport, Symport, Transport |
Ligand | Sodium |
Enzyme and pathway databases
Reactomei | R-RNO-427589, Type II Na+/Pi cotransporters R-RNO-5683826, Surfactant metabolism |
Protein family/group databases
TCDBi | 2.A.58.1.1, the phosphate:na(+) symporter (pnas) family |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium-dependent phosphate transport protein 2AShort name: Sodium-phosphate transport protein 2A Alternative name(s): Na(+)-dependent phosphate cotransporter 2A Sodium/phosphate cotransporter 2A Short name: Na(+)/Pi cotransporter 2A Short name: NaPi-2a Solute carrier family 34 member 1Imported |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3708, Slc34a1 |
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Cytoskeleton
- mitotic spindle Source: Ensembl
Cytosol
- cytosol Source: Ensembl
Endosome
- endosome Source: RGD
Nucleus
- nuclear speck Source: Ensembl
Plasma Membrane
- apical plasma membrane Source: RGD
- basolateral plasma membrane Source: RGD
- brush border membrane Source: RGD
- intrinsic component of plasma membrane Source: RGD
- plasma membrane Source: RGD
Other locations
- brush border Source: GO_Central
- cell surface Source: RGD
- integral component of membrane Source: UniProtKB-KW
- membrane raft Source: RGD
- perinuclear region of cytoplasm Source: RGD
- vesicle Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 103 | CytoplasmicSequence analysisAdd BLAST | 103 | |
Transmembranei | 104 – 125 | Helical; Name=M1Sequence analysisAdd BLAST | 22 | |
Topological domaini | 126 – 145 | ExtracellularSequence analysisAdd BLAST | 20 | |
Transmembranei | 146 – 163 | Helical; Name=M2Sequence analysisAdd BLAST | 18 | |
Topological domaini | 164 – 216 | CytoplasmicSequence analysisAdd BLAST | 53 | |
Transmembranei | 217 – 236 | Helical; Name=M3Sequence analysisAdd BLAST | 20 | |
Topological domaini | 237 – 345 | ExtracellularSequence analysisAdd BLAST | 109 | |
Transmembranei | 346 – 368 | Helical; Name=M4Sequence analysisAdd BLAST | 23 | |
Topological domaini | 369 – 410 | CytoplasmicSequence analysisAdd BLAST | 42 | |
Transmembranei | 411 – 434 | Helical; Name=M5Sequence analysisAdd BLAST | 24 | |
Topological domaini | 435 – 464 | ExtracellularSequence analysisAdd BLAST | 30 | |
Transmembranei | 465 – 485 | Helical; Name=M6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 486 – 511 | CytoplasmicSequence analysisAdd BLAST | 26 | |
Transmembranei | 512 – 532 | Helical; Name=M7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 533 – 537 | ExtracellularSequence analysis | 5 | |
Transmembranei | 538 – 559 | Helical; Name=M8Sequence analysisAdd BLAST | 22 | |
Topological domaini | 560 – 637 | CytoplasmicSequence analysisAdd BLAST | 78 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068610 | 1 – 637 | Sodium-dependent phosphate transport protein 2AAdd BLAST | 637 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 14 | PhosphoserineCombined sources | 1 | |
Modified residuei | 34 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 225 ↔ 520 | Sequence analysis | ||
Glycosylationi | 298 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 306 ↔ 334 | 1 Publication | ||
Glycosylationi | 328 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 506 | Phosphothreonine; by PKCSequence analysis | 1 | |
Modified residuei | 605 | PhosphoserineCombined sources | 1 | |
Modified residuei | 621 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 623 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q06496 |
PTM databases
GlyGeni | Q06496, 2 sites |
iPTMneti | Q06496 |
PhosphoSitePlusi | Q06496 |
Expressioni
Tissue specificityi
Kidney.
Inductioni
By low-phosphate diet.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000015262, Expressed in adult mammalian kidney and 15 other tissues |
Genevisiblei | Q06496, RN |
Interactioni
Subunit structurei
Interacts via its C-terminal region with PDZK2.
Interacts with SLC9A3R1.
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
- PDZ domain binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000031764 |
Chemistry databases
BindingDBi | Q06496 |
Structurei
Family & Domainsi
Sequence similaritiesi
Belongs to the SLC34A transporter family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QQ3I, Eukaryota |
GeneTreei | ENSGT00950000183177 |
HOGENOMi | CLU_025063_0_0_1 |
InParanoidi | Q06496 |
OMAi | ISMAGWR |
OrthoDBi | 976094at2759 |
PhylomeDBi | Q06496 |
TreeFami | TF313981 |
Family and domain databases
InterProi | View protein in InterPro IPR003841, Na/Pi_transpt IPR029848, Na/Pi_transpt_2A |
PANTHERi | PTHR10010, PTHR10010, 1 hit PTHR10010:SF21, PTHR10010:SF21, 1 hit |
Pfami | View protein in Pfam PF02690, Na_Pi_cotrans, 2 hits |
TIGRFAMsi | TIGR01013, 2a58, 1 hit |
i Sequence
Sequence statusi: Complete.
Q06496-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMSYSERLGG PAVSPLPVRG RHMVHGAAFA YVPSPQVLHR IPGTTTYAIS
60 70 80 90 100
SLSPVALTEH SCPYGEVLEC HDPLPAKLAQ EEEQKPEPRL SQKLAQVGTK
110 120 130 140 150
LLKVPLMLGF LYLFVCSLDV LSSAFQLAGG KVAGDIFKDN AILSNPVAGL
160 170 180 190 200
VVGILVTVLV QSSSTSTSII VSMVSSGLLE VSSAIPIIMG SNIGTSVTNT
210 220 230 240 250
IVALMQAGDR TDFRRAFAGA TVHDCFNWLS VLVLLPLEAA TGYLHHVTGL
260 270 280 290 300
VVASFNIRGG RDAPDLLKVI TEPFTKLIIQ LDKSVITSIA VGDESLRNHS
310 320 330 340 350
LIRIWCQPET KEASTSMSRV EAIGSLANTT MEKCNHIFVD TGLPDLAVGL
360 370 380 390 400
ILLAGSLVVL CTCLILLVKM LNSLLKGQVA NVIQKVINTD FPAPFTWVTG
410 420 430 440 450
YFAMVVGASM TFVVQSSSVF TSAITPLIGL GVISIERAYP LTLGSNIGTT
460 470 480 490 500
TTAILAALAS PREKLSSSFQ IALCHFFFNI SGILLWYPLP CTRLPIRMAK
510 520 530 540 550
ALGKRTAKYR WFAVLYLLVC FLLLPSLVFG ISMAGWQAMV GVGTPFGALL
560 570 580 590 600
AFVVLVNVLQ SRSPGHLPKW LQTWDFLPRW MHSLQPLDGL ITRATLCYAR
610 620 630
PEPRSPQLPP RVFLEELPPA TPSPRLALPA HHNATRL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13257 mRNA Translation: AAC37608.1 BC078876 mRNA Translation: AAH78876.1 |
PIRi | A48189 |
RefSeqi | NP_037162.1, NM_013030.1 |
Genome annotation databases
Ensembli | ENSRNOT00000033749; ENSRNOP00000031764; ENSRNOG00000015262 |
GeneIDi | 25548 |
KEGGi | rno:25548 |
UCSCi | RGD:3708, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13257 mRNA Translation: AAC37608.1 BC078876 mRNA Translation: AAH78876.1 |
PIRi | A48189 |
RefSeqi | NP_037162.1, NM_013030.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000031764 |
Chemistry databases
BindingDBi | Q06496 |
ChEMBLi | CHEMBL4296024 |
Protein family/group databases
TCDBi | 2.A.58.1.1, the phosphate:na(+) symporter (pnas) family |
PTM databases
GlyGeni | Q06496, 2 sites |
iPTMneti | Q06496 |
PhosphoSitePlusi | Q06496 |
Proteomic databases
PaxDbi | Q06496 |
Genome annotation databases
Ensembli | ENSRNOT00000033749; ENSRNOP00000031764; ENSRNOG00000015262 |
GeneIDi | 25548 |
KEGGi | rno:25548 |
UCSCi | RGD:3708, rat |
Organism-specific databases
CTDi | 6569 |
RGDi | 3708, Slc34a1 |
Phylogenomic databases
eggNOGi | ENOG502QQ3I, Eukaryota |
GeneTreei | ENSGT00950000183177 |
HOGENOMi | CLU_025063_0_0_1 |
InParanoidi | Q06496 |
OMAi | ISMAGWR |
OrthoDBi | 976094at2759 |
PhylomeDBi | Q06496 |
TreeFami | TF313981 |
Enzyme and pathway databases
Reactomei | R-RNO-427589, Type II Na+/Pi cotransporters R-RNO-5683826, Surfactant metabolism |
Miscellaneous databases
PROi | PR:Q06496 |
Gene expression databases
Bgeei | ENSRNOG00000015262, Expressed in adult mammalian kidney and 15 other tissues |
Genevisiblei | Q06496, RN |
Family and domain databases
InterProi | View protein in InterPro IPR003841, Na/Pi_transpt IPR029848, Na/Pi_transpt_2A |
PANTHERi | PTHR10010, PTHR10010, 1 hit PTHR10010:SF21, PTHR10010:SF21, 1 hit |
Pfami | View protein in Pfam PF02690, Na_Pi_cotrans, 2 hits |
TIGRFAMsi | TIGR01013, 2a58, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NPT2A_RAT | |
Accessioni | Q06496Primary (citable) accession number: Q06496 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families