UniProtKB - Q06486 (KC1D_RAT)
Casein kinase I isoform delta
Csnk1d
Functioni
Essential serine/threonine-protein kinase that regulates diverse cellular growth and survival processes including Wnt signaling, DNA repair and circadian rhythms. It can phosphorylate a large number of proteins. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Phosphorylates connexin-43/GJA1, MAP1A, SNAPIN, MAPT/TAU, TOP2A, DCK, HIF1A, EIF6, p53/TP53, DVL2, DVL3, ESR1, AIB1/NCOA3, DNMT1, PKD2, YAP1, PER1 and PER2. Central component of the circadian clock. In balance with PP1, determines the circadian period length through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. YAP1 phosphorylation promotes its SCF(beta-TRCP) E3 ubiquitin ligase-mediated ubiquitination and subsequent degradation. DNMT1 phosphorylation reduces its DNA-binding activity. Phosphorylation of ESR1 and AIB1/NCOA3 stimulates their activity and coactivation. Phosphorylation of DVL2 and DVL3 regulates WNT3A signaling pathway that controls neurite outgrowth. EIF6 phosphorylation promotes its nuclear export. Triggers down-regulation of dopamine receptors in the forebrain. Activates DCK in vitro by phosphorylation. TOP2A phosphorylation favors DNA cleavable complex formation. May regulate the formation of the mitotic spindle apparatus in extravillous trophoblast. Modulates connexin-43/GJA1 gap junction assembly by phosphorylation. Probably involved in lymphocyte physiology. Regulates fast synaptic transmission mediated by glutamate (By similarity).
By similarity1 PublicationCatalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 38 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 128 | Proton acceptorPROSITE-ProRule annotation1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 15 – 23 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- kinase activity Source: RGD
- protein kinase activity Source: UniProtKB
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: RGD
- protein serine kinase activity Source: RHEA
- tau-protein kinase activity Source: UniProtKB-EC
GO - Biological processi
- cellular response to nerve growth factor stimulus Source: RGD
- circadian regulation of gene expression Source: UniProtKB
- Golgi organization Source: RGD
- microtubule nucleation Source: RGD
- non-motile cilium assembly Source: RGD
- peptidyl-serine phosphorylation Source: RGD
- positive regulation of canonical Wnt signaling pathway Source: RGD
- positive regulation of non-canonical Wnt signaling pathway Source: RGD
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
- positive regulation of protein phosphorylation Source: RGD
- positive regulation of Wnt signaling pathway Source: RGD
- protein localization to centrosome Source: RGD
- protein localization to cilium Source: RGD
- protein localization to Golgi apparatus Source: RGD
- protein phosphorylation Source: RGD
- regulation of circadian rhythm Source: UniProtKB
- spindle assembly Source: RGD
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Biological rhythms, Wnt signaling pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 5301 |
Reactomei | R-RNO-204005, COPII-mediated vesicle transport R-RNO-2565942, Regulation of PLK1 Activity at G2/M Transition R-RNO-380259, Loss of Nlp from mitotic centrosomes R-RNO-380270, Recruitment of mitotic centrosome proteins and complexes R-RNO-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-RNO-380320, Recruitment of NuMA to mitotic centrosomes R-RNO-5620912, Anchoring of the basal body to the plasma membrane R-RNO-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-RNO-8854518, AURKA Activation by TPX2 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Csnk1d Synonyms:Hckid |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 71031, Csnk1d |
Subcellular locationi
Cytoskeleton
- centrosome By similarity
- spindle By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Plasma membrane
- Cell membrane By similarity
Golgi apparatus
- Golgi apparatus By similarity
Nucleus
- Nucleus By similarity
Other locations
- perinuclear region By similarity
Note: Localized at mitotic spindle microtubules, and at the centrosomes and interphase in interphase cells. Recruited to the spindle apparatus and the centrosomes in response to DNA-damage. Correct subcellular localization requires kinase activity (By similarity).By similarity
Cytoskeleton
- centrosome Source: RGD
- ciliary basal body Source: RGD
- spindle Source: RGD
- spindle microtubule Source: RGD
Golgi apparatus
- Golgi apparatus Source: RGD
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: RGD
Other locations
- cytoplasm Source: GO_Central
- neuron projection Source: RGD
- perinuclear region of cytoplasm Source: RGD
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000192836 | 1 – 415 | Casein kinase I isoform deltaAdd BLAST | 415 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 328 | PhosphoserineBy similarity | 1 | |
Modified residuei | 331 | PhosphoserineBy similarity | 1 | |
Modified residuei | 370 | Phosphoserine; by PKA1 Publication | 1 | |
Modified residuei | 375 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 382 | PhosphoserineCombined sources | 1 | |
Modified residuei | 383 | PhosphoserineBy similarity | 1 | |
Modified residuei | 384 | PhosphoserineBy similarity | 1 | |
Modified residuei | 407 | PhosphoserineBy similarity | 1 | |
Modified residuei | 411 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
jPOSTi | Q06486 |
PaxDbi | Q06486 |
PTM databases
iPTMneti | Q06486 |
PhosphoSitePlusi | Q06486 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000036676, Expressed in testis and 21 other tissues |
Genevisiblei | Q06486, RN |
Interactioni
Subunit structurei
Monomer (By similarity).
Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, ARTNL/BMAL1 or ARTNL2/BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins (By similarity).
Interacts with DNMT1 (By similarity).
Interacts directly with PER1 and PER2 which may lead to their degradation (By similarity).
Interacts with MAPT/TAU, SNAPIN, DBNDD2, AIB1/NCOA3 and ESR1 (By similarity).
Interacts with AKAP9/AKAP450; this interaction promotes centrosomal subcellular location (By similarity). Binds to tubulins in mitotic cells upon DNA damage (By similarity).
Interacts with GJA1 (By similarity).
Interacts with MAP1A (PubMed:15961172).
Interacts with DDX3X; this interaction enhances CSNK1D kinase activity in vitro, but it is unclear whether this interaction is physiologically relevant (By similarity).
By similarity1 PublicationBinary interactionsi
Q06486
With | #Exp. | IntAct |
---|---|---|
MDM2 [Q00987] from Homo sapiens. | 2 | EBI-2910316,EBI-389668 |
Isoform 2 [Q06486-2]
With | #Exp. | IntAct |
---|---|---|
Snapin [Q9Z266] from Mus musculus. | 4 | EBI-7088890,EBI-6170320 |
Protein-protein interaction databases
BioGRIDi | 249077, 14 interactors |
IntActi | Q06486, 12 interactors |
MINTi | Q06486 |
STRINGi | 10116.ENSRNOP00000015178 |
Chemistry databases
BindingDBi | Q06486 |
Structurei
Secondary structure
3D structure databases
SMRi | Q06486 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q06486 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 277 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 269 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 278 – 364 | Centrosomal localization signal (CLS)By similarityAdd BLAST | 87 | |
Regioni | 301 – 415 | DisorderedSequence analysisAdd BLAST | 115 | |
Regioni | 317 – 342 | AutoinhibitoryAdd BLAST | 26 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 301 – 320 | Basic and acidic residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 342 – 359 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 380 – 415 | Polar residuesSequence analysisAdd BLAST | 36 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1164, Eukaryota |
GeneTreei | ENSGT00940000153536 |
HOGENOMi | CLU_019279_2_2_1 |
InParanoidi | Q06486 |
OMAi | IYFCRGS |
OrthoDBi | 1097975at2759 |
PhylomeDBi | Q06486 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MELRVGNRYR LGRKIGSGSF GDIYLGTDIA AGEEVAIKLE CVKTKHPQLH
60 70 80 90 100
IESKIYKMMQ GGVGIPTIRW CGAEGDYNVM VMELLGPSLE DLFNFCSRKF
110 120 130 140 150
SLKTVLLLAD QMISRIEYIH SKNFIHRDVK PDNFLMGLGK KGNLVYIIDF
160 170 180 190 200
GLAKKYRDAR THQHIPYREN KNLTGTARYA SINTHLGIEQ SRRDDLESLG
210 220 230 240 250
YVLMYFNLGS LPWQGLKAAT KRQKYERISE KKMSTPIEVL CKGYPSEFAT
260 270 280 290 300
YLNFCRSLRF DDKPDYSYLR QLFRNLFHRQ GFSYDYVFDW NMLKFGASRA
310 320 330 340 350
ADDAERERRD REERLRHSRN PATRGLPSTA SGRLRGTQEV APPTPLTPTS
360 370 380 390 400
HTANTSPRPV SGMERERKVS MRLHRGAPVN VSSSDLTGRQ DTSRMSTSQI
410
PGRVASSGLQ SVVHR
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010255 | 400 – 415 | IPGRV…SVVHR → RSRDMASLRLHAARQGARCR PQRPRRTTY in isoform 2. 1 PublicationAdd BLAST | 16 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07578 mRNA Translation: AAA40934.1 AB063114 mRNA Translation: BAB60852.1 |
PIRi | A46002 |
RefSeqi | NP_620691.2, NM_139060.3 [Q06486-1] |
Genome annotation databases
Ensembli | ENSRNOT00000015178; ENSRNOP00000015178; ENSRNOG00000036676 [Q06486-2] ENSRNOT00000054936; ENSRNOP00000051819; ENSRNOG00000036676 [Q06486-1] |
GeneIDi | 64462 |
KEGGi | rno:64462 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07578 mRNA Translation: AAA40934.1 AB063114 mRNA Translation: BAB60852.1 |
PIRi | A46002 |
RefSeqi | NP_620691.2, NM_139060.3 [Q06486-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CKI | X-ray | 2.30 | A/B | 1-317 | [»] | |
1CKJ | X-ray | 2.46 | A/B | 1-317 | [»] | |
SMRi | Q06486 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 249077, 14 interactors |
IntActi | Q06486, 12 interactors |
MINTi | Q06486 |
STRINGi | 10116.ENSRNOP00000015178 |
Chemistry databases
BindingDBi | Q06486 |
ChEMBLi | CHEMBL4484 |
PTM databases
iPTMneti | Q06486 |
PhosphoSitePlusi | Q06486 |
Proteomic databases
jPOSTi | Q06486 |
PaxDbi | Q06486 |
Genome annotation databases
Ensembli | ENSRNOT00000015178; ENSRNOP00000015178; ENSRNOG00000036676 [Q06486-2] ENSRNOT00000054936; ENSRNOP00000051819; ENSRNOG00000036676 [Q06486-1] |
GeneIDi | 64462 |
KEGGi | rno:64462 |
Organism-specific databases
CTDi | 1453 |
RGDi | 71031, Csnk1d |
Phylogenomic databases
eggNOGi | KOG1164, Eukaryota |
GeneTreei | ENSGT00940000153536 |
HOGENOMi | CLU_019279_2_2_1 |
InParanoidi | Q06486 |
OMAi | IYFCRGS |
OrthoDBi | 1097975at2759 |
PhylomeDBi | Q06486 |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 5301 |
Reactomei | R-RNO-204005, COPII-mediated vesicle transport R-RNO-2565942, Regulation of PLK1 Activity at G2/M Transition R-RNO-380259, Loss of Nlp from mitotic centrosomes R-RNO-380270, Recruitment of mitotic centrosome proteins and complexes R-RNO-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-RNO-380320, Recruitment of NuMA to mitotic centrosomes R-RNO-5620912, Anchoring of the basal body to the plasma membrane R-RNO-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-RNO-8854518, AURKA Activation by TPX2 |
Miscellaneous databases
EvolutionaryTracei | Q06486 |
PROi | PR:Q06486 |
Gene expression databases
Bgeei | ENSRNOG00000036676, Expressed in testis and 21 other tissues |
Genevisiblei | Q06486, RN |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KC1D_RAT | |
Accessioni | Q06486Primary (citable) accession number: Q06486 Secondary accession number(s): Q99KK4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | May 10, 2004 | |
Last modified: | February 23, 2022 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families