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Protein

[PSI+] inducibility protein 3

Gene

PIN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Overproduction promotes the de novo induction of the [PSI+] prion form of SUP35. The prion-inducing effect depends on the association with the actin cytoskeleton. Also implicated in prion maintenance during heat stress.1 Publication

Miscellaneous

Present with 2190 molecules/cell in log phase SD medium.1 Publication
Although this protein promotes prion formation and it has a Asn/Gln-rich prion-like domain, it does not seem to have a prion form by itself.

GO - Biological processi

  • negative regulation of Arp2/3 complex-mediated actin nucleation Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-34285-MONOMER

Protein family/group databases

MoonDBiQ06449 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
[PSI+] inducibility protein 3
Alternative name(s):
LAS seventeen-binding protein 2
Short name:
LAS17-binding protein 2
Gene namesi
Name:PIN3
Synonyms:LSB2
Ordered Locus Names:YPR154W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

SGDiS000006358 PIN3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi80K → R: Abolishes formation of ubiquitinated protein forms. 1 Publication1
Mutagenesisi91W → S: Abolishes interaction with LAS17, but not with SUP35. Blocks colocalization with actin, aggregation, and prion-inducing ability. 1 Publication1
Mutagenesisi124 – 125PP → AA: Abolishes RSP5 binding site and consequently ubiquitination. 1 Publication2
Mutagenesisi174 – 175QQ → AA: Reduces, but does not abolish the ability to promote [PSI+] induction. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002686962 – 215[PSI+] inducibility protein 3Add BLAST214

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei52PhosphoserineCombined sources1
Modified residuei55PhosphoserineCombined sources1
Cross-linki80Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Post-translational modificationi

Ubiquitinated by RSP5. Ubiquitination reduces the protein abundance and its prion-inducing ability.2 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ06449
PaxDbiQ06449
PRIDEiQ06449

PTM databases

iPTMnetiQ06449

Expressioni

Inductioni

By heat shock. Can thereby reach physiological protein levels high enough to promote prion-formation.1 Publication

Interactioni

Subunit structurei

Interacts with LAS17, RSP5 and SUP35.3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi36321, 117 interactors
DIPiDIP-6257N
IntActiQ06449, 45 interactors
MINTiQ06449
STRINGi4932.YPR154W

Structurei

Secondary structure

1215
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ06449
SMRiQ06449
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06449

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 113SH3PROSITE-ProRule annotationAdd BLAST60

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi124 – 127PY motif4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi122 – 125Poly-Pro4
Compositional biasi126 – 181Asn/Gln-richAdd BLAST56

Domaini

The PY motif is recognized directly by the WW domains of RSP5.

Sequence similaritiesi

Belongs to the LSB1 family.Curated

Keywords - Domaini

SH3 domain

Phylogenomic databases

GeneTreeiENSGT00910000146264
HOGENOMiHOG000195703
InParanoidiQ06449
KOiK20522
OMAiKPSAEWY
OrthoDBiEOG092C454D

Family and domain databases

InterProiView protein in InterPro
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00018 SH3_1, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06449-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSASLINRSL TNIRTELDFL KGSNVISNDV YDQINKSLPA KWDPANAPRN
60 70 80 90 100
ASPASLEYVE ALYQFDPQQD GDLGLKPGDK VQLLEKLSPE WYKGSCNGRT
110 120 130 140 150
GIFPANYVKP AFSGSNGPSN LPPPPQYKAQ ELQQIPTQNS AASSYQQQPF
160 170 180 190 200
PPPSTNYYQQ PQQQPQQAPP PQQQQQQQQH QSSHSHLKSF GSKLGNAAIF
210
GAGASIGSDI VNNIF
Length:215
Mass (Da):23,539
Last modified:November 1, 1996 - v1
Checksum:i37C95425B02FB284
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28371 Genomic DNA Translation: AAB68051.1
AY692989 Genomic DNA Translation: AAT93008.1
BK006949 Genomic DNA Translation: DAA11566.1
PIRiS61138
RefSeqiNP_015480.1, NM_001184251.1

Genome annotation databases

EnsemblFungiiYPR154W; YPR154W; YPR154W
GeneIDi856277
KEGGisce:YPR154W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28371 Genomic DNA Translation: AAB68051.1
AY692989 Genomic DNA Translation: AAT93008.1
BK006949 Genomic DNA Translation: DAA11566.1
PIRiS61138
RefSeqiNP_015480.1, NM_001184251.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YNZX-ray2.20A57-112[»]
1ZX6X-ray1.60A57-112[»]
ProteinModelPortaliQ06449
SMRiQ06449
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36321, 117 interactors
DIPiDIP-6257N
IntActiQ06449, 45 interactors
MINTiQ06449
STRINGi4932.YPR154W

Protein family/group databases

MoonDBiQ06449 Predicted

PTM databases

iPTMnetiQ06449

Proteomic databases

MaxQBiQ06449
PaxDbiQ06449
PRIDEiQ06449

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR154W; YPR154W; YPR154W
GeneIDi856277
KEGGisce:YPR154W

Organism-specific databases

SGDiS000006358 PIN3

Phylogenomic databases

GeneTreeiENSGT00910000146264
HOGENOMiHOG000195703
InParanoidiQ06449
KOiK20522
OMAiKPSAEWY
OrthoDBiEOG092C454D

Enzyme and pathway databases

BioCyciYEAST:G3O-34285-MONOMER

Miscellaneous databases

EvolutionaryTraceiQ06449
PROiPR:Q06449

Family and domain databases

InterProiView protein in InterPro
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00018 SH3_1, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPIN3_YEAST
AccessioniPrimary (citable) accession number: Q06449
Secondary accession number(s): D6W4F0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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