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Protein

Calcium-binding protein NCS-1

Gene

FRQ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulator of phosphatidylinositol 4-kinase PIK1.1 Publication

Miscellaneous

Binds 3 calcium ions via the second, third and fourth EF-hand.
Present with 7160 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi73 – 841Add BLAST12
Calcium bindingi109 – 1202Add BLAST12
Calcium bindingi157 – 1683Add BLAST12

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • enzyme activator activity Source: SGD

GO - Biological processi

  • positive regulation of 1-phosphatidylinositol 4-kinase activity Source: SGD
  • regulation of signal transduction Source: SGD

Keywordsi

LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29923-MONOMER
ReactomeiR-SCE-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-SCE-451308 Activation of Ca-permeable Kainate Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding protein NCS-1
Gene namesi
Name:FRQ1
Synonyms:NCS1
Ordered Locus Names:YDR373W
ORF Names:D9481.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR373W
SGDiS000002781 FRQ1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: Abolishes N-myristoylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000737952 – 190Calcium-binding protein NCS-1Add BLAST189

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiQ06389
PaxDbiQ06389
PRIDEiQ06389

PTM databases

iPTMnetiQ06389

Interactioni

Subunit structurei

Binds PIK1.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi32432, 295 interactors
DIPiDIP-2733N
IntActiQ06389, 38 interactors
MINTiQ06389
STRINGi4932.YDR373W

Structurei

Secondary structure

1190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ06389
SMRiQ06389
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06389

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 59EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini60 – 95EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini96 – 131EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini144 – 179EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Belongs to the recoverin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00760000118820
HOGENOMiHOG000233019
InParanoidiQ06389
KOiK19932
OMAiKRELQQW
OrthoDBiEOG092C4XFN

Family and domain databases

CDDicd00051 EFh, 2 hits
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13499 EF-hand_7, 1 hit
PF13833 EF-hand_8, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06389-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGAKTSKLSK DDLTCLKQST YFDRREIQQW HKGFLRDCPS GQLAREDFVK
60 70 80 90 100
IYKQFFPFGS PEDFANHLFT VFDKDNNGFI HFEEFITVLS TTSRGTLEEK
110 120 130 140 150
LSWAFELYDL NHDGYITFDE MLTIVASVYK MMGSMVTLNE DEATPEMRVK
160 170 180 190
KIFKLMDKNE DGYITLDEFR EGSKVDPSII GALNLYDGLI
Length:190
Mass (Da):22,011
Last modified:January 23, 2007 - v2
Checksum:i5E9D7F48F8AADA21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28373 Genomic DNA Translation: AAB64809.1
BK006938 Genomic DNA Translation: DAA12214.1
PIRiS61168
RefSeqiNP_010661.3, NM_001180681.3

Genome annotation databases

EnsemblFungiiYDR373W; YDR373W; YDR373W
GeneIDi851979
KEGGisce:YDR373W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28373 Genomic DNA Translation: AAB64809.1
BK006938 Genomic DNA Translation: DAA12214.1
PIRiS61168
RefSeqiNP_010661.3, NM_001180681.3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FPWNMR-A1-190[»]
2JU0NMR-A1-190[»]
ProteinModelPortaliQ06389
SMRiQ06389
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32432, 295 interactors
DIPiDIP-2733N
IntActiQ06389, 38 interactors
MINTiQ06389
STRINGi4932.YDR373W

PTM databases

iPTMnetiQ06389

Proteomic databases

MaxQBiQ06389
PaxDbiQ06389
PRIDEiQ06389

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR373W; YDR373W; YDR373W
GeneIDi851979
KEGGisce:YDR373W

Organism-specific databases

EuPathDBiFungiDB:YDR373W
SGDiS000002781 FRQ1

Phylogenomic databases

GeneTreeiENSGT00760000118820
HOGENOMiHOG000233019
InParanoidiQ06389
KOiK19932
OMAiKRELQQW
OrthoDBiEOG092C4XFN

Enzyme and pathway databases

BioCyciYEAST:G3O-29923-MONOMER
ReactomeiR-SCE-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-SCE-451308 Activation of Ca-permeable Kainate Receptor

Miscellaneous databases

EvolutionaryTraceiQ06389
PROiPR:Q06389

Family and domain databases

CDDicd00051 EFh, 2 hits
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13499 EF-hand_7, 1 hit
PF13833 EF-hand_8, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNCS1_YEAST
AccessioniPrimary (citable) accession number: Q06389
Secondary accession number(s): D6VT04
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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