UniProtKB - Q06385 (VPS74_YEAST)
Vacuolar protein sorting-associated protein 74
VPS74
Functioni
Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in vacuolar protein sorting.
3 PublicationsMiscellaneous
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 88 | PtdIns4PCurated | 1 | |
Binding sitei | 97 | PtdIns4PCurated | 1 | |
Binding sitei | 178 | PtdIns4PCurated | 1 | |
Binding sitei | 181 | PtdIns4PCurated | 1 |
GO - Molecular functioni
- enzyme binding Source: SGD
- phosphatidylinositol-4-phosphate binding Source: UniProtKB
GO - Biological processi
- endoplasmic reticulum unfolded protein response Source: SGD
- Golgi organization Source: GO_Central
- Golgi to plasma membrane protein transport Source: GO_Central
- Golgi vesicle budding Source: GO_Central
- protein N-linked glycosylation Source: CACAO
- protein O-linked mannosylation Source: CACAO
- protein retention in Golgi apparatus Source: SGD
- regulation of phosphatidylinositol dephosphorylation Source: SGD
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: SGD
Keywordsi
Biological process | Protein transport, Transport |
Ligand | Lipid-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein sorting-associated protein 74 |
Gene namesi | Name:VPS74 Ordered Locus Names:YDR372C ORF Names:D9481.15 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002780, VPS74 |
VEuPathDBi | FungiDB:YDR372C |
Subcellular locationi
Golgi apparatus
- Golgi stack membrane 3 Publications; Peripheral membrane protein 3 Publications; Cytoplasmic side 3 Publications
Note: Phosphatidylinositol 4-phosphate-binding and oligomerization participate in the recruitment onto Golgi membranes.
Cytosol
- cytosol Source: SGD
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi cisterna Source: GO_Central
- Golgi cisterna membrane Source: UniProtKB-SubCell
- Golgi medial cisterna Source: SGD
- Golgi membrane Source: GOC
- trans-Golgi network Source: CACAO
Nucleus
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
- extrinsic component of membrane Source: SGD
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 6 – 8 | RRR → AAA: Loss of coatomer-binding. 1 Publication | 3 | |
Mutagenesisi | 19 | S → A: Alters phosphorylation but has not effect on Golgi enzymes localization. | 1 | |
Mutagenesisi | 88 | W → A: Abolishes phosphoinositide binding and Golgi localization; when associated with A-97. 1 Publication | 1 | |
Mutagenesisi | 97 | R → A: Abolishes phosphoinositide binding and Golgi localization; when associated with A-88. 1 Publication | 1 | |
Mutagenesisi | 178 | K → A: Abolishes phosphoinositide binding and Golgi localization; when associated with A-181. 1 Publication | 1 | |
Mutagenesisi | 181 | R → A: Abolishes phosphoinositide binding and Golgi localization; when associated with A-178. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000123823 | 1 – 345 | Vacuolar protein sorting-associated protein 74Add BLAST | 345 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 14 | PhosphoserineCombined sources | 1 | |
Modified residuei | 19 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q06385 |
PaxDbi | Q06385 |
PRIDEi | Q06385 |
PTM databases
iPTMneti | Q06385 |
Interactioni
Subunit structurei
Homotetramer.
Interacts with coatomer complex. May interact with ARF1.
1 PublicationGO - Molecular functioni
- enzyme binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 32431, 224 interactors |
DIPi | DIP-1768N |
IntActi | Q06385, 9 interactors |
MINTi | Q06385 |
STRINGi | 4932.YDR372C |
Miscellaneous databases
RNActi | Q06385, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q06385 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q06385 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 26 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 197 – 208 | Beta-hairpin required for oligomerizationAdd BLAST | 12 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3983, Eukaryota |
GeneTreei | ENSGT00390000007153 |
HOGENOMi | CLU_036311_1_0_1 |
InParanoidi | Q06385 |
OMAi | LMKINYQ |
Family and domain databases
Gene3Di | 1.10.3630.10, 1 hit |
InterProi | View protein in InterPro IPR008628, GPP34-like IPR038261, GPP34-like_sf |
PANTHERi | PTHR12704, PTHR12704, 1 hit |
Pfami | View protein in Pfam PF05719, GPP34, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSTLQRRRVN RADSGDTSSI HSSANNTKGD KIANIAVDGD DDNGTNKKIA
60 70 80 90 100
YDPEESKLRD NINIPTLTLM EEVLLMGLRD REGYLSFWND SISYALRGCI
110 120 130 140 150
IIELALRGKI RILDDSARKR FDLSERLIEV IDSSKTGEVL LDETLQLMKN
160 170 180 190 200
DEPLSISNWI DLLSGETWNL LKINYQLKQV RERLAKGLVD KGVLRTEMKN
210 220 230 240 250
FFLFDMATHP IADASCKEAI KRRVLSVLVS RNMELSYNEY FPETTSFKII
260 270 280 290 300
RTLALICGSY GANVLENVLT TLEYEKRDKA ISRAEEIMAQ FSQYPFDLEK
310 320 330 340
ETELGVSVNL NKEVKEEIEN NPGHDLQLEV IAGVFEVFSR MDMLL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28373 Genomic DNA Translation: AAB64808.1 BK006938 Genomic DNA Translation: DAA12213.1 |
PIRi | S61167 |
RefSeqi | NP_010660.1, NM_001180680.1 |
Genome annotation databases
EnsemblFungii | YDR372C_mRNA; YDR372C; YDR372C |
GeneIDi | 851978 |
KEGGi | sce:YDR372C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28373 Genomic DNA Translation: AAB64808.1 BK006938 Genomic DNA Translation: DAA12213.1 |
PIRi | S61167 |
RefSeqi | NP_010660.1, NM_001180680.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ZIH | X-ray | 2.80 | A/B/C/D | 1-345 | [»] | |
2ZII | X-ray | 3.05 | A/B/C/D | 60-345 | [»] | |
4TU3 | X-ray | 3.19 | A | 1-345 | [»] | |
SMRi | Q06385 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32431, 224 interactors |
DIPi | DIP-1768N |
IntActi | Q06385, 9 interactors |
MINTi | Q06385 |
STRINGi | 4932.YDR372C |
PTM databases
iPTMneti | Q06385 |
Proteomic databases
MaxQBi | Q06385 |
PaxDbi | Q06385 |
PRIDEi | Q06385 |
Genome annotation databases
EnsemblFungii | YDR372C_mRNA; YDR372C; YDR372C |
GeneIDi | 851978 |
KEGGi | sce:YDR372C |
Organism-specific databases
SGDi | S000002780, VPS74 |
VEuPathDBi | FungiDB:YDR372C |
Phylogenomic databases
eggNOGi | KOG3983, Eukaryota |
GeneTreei | ENSGT00390000007153 |
HOGENOMi | CLU_036311_1_0_1 |
InParanoidi | Q06385 |
OMAi | LMKINYQ |
Miscellaneous databases
EvolutionaryTracei | Q06385 |
PROi | PR:Q06385 |
RNActi | Q06385, protein |
Family and domain databases
Gene3Di | 1.10.3630.10, 1 hit |
InterProi | View protein in InterPro IPR008628, GPP34-like IPR038261, GPP34-like_sf |
PANTHERi | PTHR12704, PTHR12704, 1 hit |
Pfami | View protein in Pfam PF05719, GPP34, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VPS74_YEAST | |
Accessioni | Q06385Primary (citable) accession number: Q06385 Secondary accession number(s): D6VT03 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families