UniProtKB - Q06335 (APLP2_MOUSE)
Amyloid beta precursor like protein 2
Aplp2
Functioni
May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein (By similarity).
Modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts.
By similarity1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 163 | CopperPROSITE-ProRule annotation | 1 | |
Metal bindingi | 167 | CopperPROSITE-ProRule annotation | 1 | |
Metal bindingi | 184 | CopperPROSITE-ProRule annotation | 1 | |
Sitei | 186 | Required for Cu(2+) reductionPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- heparin binding Source: InterPro
- identical protein binding Source: MGI
- transition metal ion binding Source: InterPro
GO - Biological processi
- cellular copper ion homeostasis Source: MGI
- cholesterol metabolic process Source: MGI
- extracellular matrix organization Source: MGI
- forebrain development Source: MGI
- locomotory behavior Source: MGI
- mating behavior Source: MGI
- midbrain development Source: MGI
- neuromuscular process controlling balance Source: MGI
- regulation of epidermal growth factor-activated receptor activity Source: MGI
- regulation of protein binding Source: MGI
- suckling behavior Source: MGI
Keywordsi
Molecular function | DNA-binding |
Ligand | Copper, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-8957275, Post-translational protein phosphorylation |
Names & Taxonomyi
Protein namesi | Recommended name: Amyloid beta precursor like protein 2By similarityAlternative name(s): Amyloid beta (A4) precursor-like protein 2Imported Amyloid protein homologCurated Amyloid-like protein 2By similarity Short name: APLP-2 CDEI box-binding protein Short name: CDEBP Sperm membrane protein YWK-IIBy similarity |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88047, Aplp2 |
VEuPathDBi | HostDB:ENSMUSG00000031996 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Single-pass type I membrane protein Curated
Nucleus
- Nucleus Curated
Nucleus
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 32 – 636 | ExtracellularSequence analysisAdd BLAST | 605 | |
Transmembranei | 637 – 660 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 661 – 707 | CytoplasmicSequence analysisAdd BLAST | 47 |
Keywords - Cellular componenti
Cell membrane, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | Sequence analysisAdd BLAST | 31 | |
ChainiPRO_0000000208 | 32 – 707 | Amyloid beta precursor like protein 2Add BLAST | 676 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 56 ↔ 80 | PROSITE-ProRule annotation | ||
Disulfide bondi | 91 ↔ 133 | PROSITE-ProRule annotation | ||
Disulfide bondi | 116 ↔ 123 | PROSITE-ProRule annotation | ||
Disulfide bondi | 149 ↔ 203 | PROSITE-ProRule annotation | ||
Disulfide bondi | 160 ↔ 190 | PROSITE-ProRule annotation | ||
Disulfide bondi | 174 ↔ 202 | PROSITE-ProRule annotation | ||
Modified residuei | 216 | PhosphoserineBy similarity | 1 | |
Glycosylationi | 485 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 534 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | Q06335 |
MaxQBi | Q06335 |
PaxDbi | Q06335 |
PeptideAtlasi | Q06335 |
PRIDEi | Q06335 |
ProteomicsDBi | 296378 [Q06335-1] 296379 [Q06335-2] |
PTM databases
GlyGeni | Q06335, 1 site |
iPTMneti | Q06335 |
PhosphoSitePlusi | Q06335 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000031996, Expressed in white adipose tissue and 325 other tissues |
ExpressionAtlasi | Q06335, baseline and differential |
Genevisiblei | Q06335, MM |
Interactioni
Subunit structurei
Interacts with CPEB1.
Interacts (via NPXY motif) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif.
Interacts (via cytoplasmic domain) with APBB2/FE65L (By similarity).
Interacts (via intracellular domain) with APBB3/FE65L2 (By similarity).
By similarity2 PublicationsBinary interactionsi
Q06335
With | #Exp. | IntAct |
---|---|---|
App [P12023] | 3 | EBI-446708,EBI-78814 |
GO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 198154, 7 interactors |
IntActi | Q06335, 11 interactors |
MINTi | Q06335 |
STRINGi | 10090.ENSMUSP00000072428 |
Miscellaneous databases
RNActi | Q06335, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 46 – 205 | E1PROSITE-ProRule annotationAdd BLAST | 160 | |
Domaini | 317 – 508 | E2PROSITE-ProRule annotationAdd BLAST | 192 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 46 – 139 | GFLD subdomainPROSITE-ProRule annotationAdd BLAST | 94 | |
Regioni | 147 – 205 | CuBD subdomainPROSITE-ProRule annotationAdd BLAST | 59 | |
Regioni | 208 – 298 | DisorderedSequence analysisAdd BLAST | 91 | |
Regioni | 539 – 575 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 693 – 707 | Interaction with DAB21 PublicationAdd BLAST | 15 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 694 – 699 | NPXY motif | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 215 – 238 | Acidic residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 245 – 270 | Acidic residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 271 – 286 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3540, Eukaryota |
GeneTreei | ENSGT00530000063252 |
InParanoidi | Q06335 |
Family and domain databases
Gene3Di | 1.20.120.770, 1 hit 2.30.29.30, 1 hit 3.30.1490.140, 1 hit 3.90.570.10, 1 hit |
InterProi | View protein in InterPro IPR036669, Amyloid_Cu-bd_sf IPR008155, Amyloid_glyco IPR011178, Amyloid_glyco_Cu-bd IPR024329, Amyloid_glyco_E2_domain IPR008154, Amyloid_glyco_extra IPR015849, Amyloid_glyco_heparin-bd IPR036454, Amyloid_glyco_heparin-bd_sf IPR019745, Amyloid_glyco_intracell_CS IPR019543, APP_amyloid_C IPR019744, APP_CUBD_CS IPR036176, E2_sf IPR011993, PH-like_dom_sf |
PANTHERi | PTHR23103, PTHR23103, 2 hits |
Pfami | View protein in Pfam PF10515, APP_amyloid, 1 hit PF12924, APP_Cu_bd, 1 hit PF12925, APP_E2, 1 hit PF02177, APP_N, 1 hit |
PRINTSi | PR00203, AMYLOIDA4 |
SMARTi | View protein in SMART SM00006, A4_EXTRA, 1 hit |
SUPFAMi | SSF109843, SSF109843, 1 hit SSF56491, SSF56491, 1 hit SSF89811, SSF89811, 1 hit |
PROSITEi | View protein in PROSITE PS00319, APP_CUBD, 1 hit PS51869, APP_E1, 1 hit PS51870, APP_E2, 1 hit PS00320, APP_INTRA, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAATGTAAAA ATGKLLVLLL LGLTAPAAAL AGYIEALAAN AGTGFAVAEP
60 70 80 90 100
QIAMFCGKLN MHVNIQTGKW EPDPTGTKSC LGTKEEVLQY CQEIYPELQI
110 120 130 140 150
TNVMEANQPV NIDSWCRRDK RQCKSHIVIP FKCLVGEFVS DVLLVPDNCQ
160 170 180 190 200
FFHQERMEVC EKHQRWHTLV KEACLTEGLT LYSYGMLLPC GVDQFHGTEY
210 220 230 240 250
VCCPQTKTVD SDSTMSKEEE EEEEDEEDEE EDYDLDKSEF PTEADLEDFT
260 270 280 290 300
EAAADEEEED EEEGEEVVED RDYYYDPFKG DDYNEENPTE PSSEGTISDK
310 320 330 340 350
EIVHDVKVPP TPLPTNDVDV YFETSADDNE HARFQKAKEQ LEIRHRNRMD
360 370 380 390 400
RVKKEWEEAE LQAKNLPKTE RQTLIQHFQA MVKALEKEAA SEKQQLVETH
410 420 430 440 450
LARVEAMLND RRRIALENYL AALQSDPPRP HRILQALRRY VRAENKDRLH
460 470 480 490 500
TIRHYQHVLA VDPEKAAQMK SQVMTHLHVI EERRNQSLSL LYKVPYVAQE
510 520 530 540 550
IQEEIDELLQ EQRADMDQFT SSISENPVDV RVSSEESEEI PPFHPLHPFP
560 570 580 590 600
SLSENEDTQP ELYHPMKKGS GMAEQDGGLI GAEEKVINSK NKMDENMVID
610 620 630 640 650
ETLDVKEMIF NAERVGGLEE EPESVGPLRE DFSLSSNALI GLLVIAVAIA
660 670 680 690 700
TVIVISLVML RKRQYGTISH GIVEVDPMLT PEERHLNKMQ NHGYENPTYK
YLEQMQI
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ60709 | Q60709_MOUSE | Amyloid beta precursor-like protein... | Aplp2 APLP2 | 751 | Annotation score: | ||
A0A1L1SS44 | A0A1L1SS44_MOUSE | Amyloid beta precursor-like protein... | Aplp2 | 624 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 55 | F → L in CAA80306 (Ref. 1) Curated | 1 | |
Sequence conflicti | 185 – 189 | GMLLP → MACCC in AAA20039 (PubMed:1482349).Curated | 5 | |
Sequence conflicti | 322 | F → L in CAA80306 (Ref. 1) Curated | 1 | |
Sequence conflicti | 322 | F → L in AAA20039 (PubMed:1482349).Curated | 1 | |
Sequence conflicti | 489 | S → T in CAA80306 (Ref. 1) Curated | 1 | |
Sequence conflicti | 489 | S → T in AAA20039 (PubMed:1482349).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041386 | 557 – 568 | Missing in isoform 2. 2 PublicationsAdd BLAST | 12 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z22592 mRNA Translation: CAA80306.1 BC051999 mRNA Translation: AAH51999.1 M97216 mRNA Translation: AAA20039.1 U34291 Genomic DNA Translation: AAC52318.1 |
CCDSi | CCDS52748.1 [Q06335-1] CCDS90528.1 [Q06335-2] |
PIRi | JC1404 S38344 |
RefSeqi | NP_001095926.1, NM_001102456.1 [Q06335-1] |
Genome annotation databases
Ensembli | ENSMUST00000079758; ENSMUSP00000078694; ENSMUSG00000031996 [Q06335-1] ENSMUST00000217641; ENSMUSP00000149023; ENSMUSG00000031996 [Q06335-2] |
GeneIDi | 11804 |
KEGGi | mmu:11804 |
UCSCi | uc009orl.1, mouse [Q06335-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z22592 mRNA Translation: CAA80306.1 BC051999 mRNA Translation: AAH51999.1 M97216 mRNA Translation: AAA20039.1 U34291 Genomic DNA Translation: AAC52318.1 |
CCDSi | CCDS52748.1 [Q06335-1] CCDS90528.1 [Q06335-2] |
PIRi | JC1404 S38344 |
RefSeqi | NP_001095926.1, NM_001102456.1 [Q06335-1] |
3D structure databases
SMRi | Q06335 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198154, 7 interactors |
IntActi | Q06335, 11 interactors |
MINTi | Q06335 |
STRINGi | 10090.ENSMUSP00000072428 |
PTM databases
GlyGeni | Q06335, 1 site |
iPTMneti | Q06335 |
PhosphoSitePlusi | Q06335 |
Proteomic databases
jPOSTi | Q06335 |
MaxQBi | Q06335 |
PaxDbi | Q06335 |
PeptideAtlasi | Q06335 |
PRIDEi | Q06335 |
ProteomicsDBi | 296378 [Q06335-1] 296379 [Q06335-2] |
Protocols and materials databases
Antibodypediai | 33074, 379 antibodies from 35 providers |
DNASUi | 11804 |
Genome annotation databases
Ensembli | ENSMUST00000079758; ENSMUSP00000078694; ENSMUSG00000031996 [Q06335-1] ENSMUST00000217641; ENSMUSP00000149023; ENSMUSG00000031996 [Q06335-2] |
GeneIDi | 11804 |
KEGGi | mmu:11804 |
UCSCi | uc009orl.1, mouse [Q06335-1] |
Organism-specific databases
CTDi | 334 |
MGIi | MGI:88047, Aplp2 |
VEuPathDBi | HostDB:ENSMUSG00000031996 |
Phylogenomic databases
eggNOGi | KOG3540, Eukaryota |
GeneTreei | ENSGT00530000063252 |
InParanoidi | Q06335 |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-8957275, Post-translational protein phosphorylation |
Miscellaneous databases
BioGRID-ORCSi | 11804, 2 hits in 64 CRISPR screens |
ChiTaRSi | Aplp2, mouse |
PROi | PR:Q06335 |
RNActi | Q06335, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031996, Expressed in white adipose tissue and 325 other tissues |
ExpressionAtlasi | Q06335, baseline and differential |
Genevisiblei | Q06335, MM |
Family and domain databases
Gene3Di | 1.20.120.770, 1 hit 2.30.29.30, 1 hit 3.30.1490.140, 1 hit 3.90.570.10, 1 hit |
InterProi | View protein in InterPro IPR036669, Amyloid_Cu-bd_sf IPR008155, Amyloid_glyco IPR011178, Amyloid_glyco_Cu-bd IPR024329, Amyloid_glyco_E2_domain IPR008154, Amyloid_glyco_extra IPR015849, Amyloid_glyco_heparin-bd IPR036454, Amyloid_glyco_heparin-bd_sf IPR019745, Amyloid_glyco_intracell_CS IPR019543, APP_amyloid_C IPR019744, APP_CUBD_CS IPR036176, E2_sf IPR011993, PH-like_dom_sf |
PANTHERi | PTHR23103, PTHR23103, 2 hits |
Pfami | View protein in Pfam PF10515, APP_amyloid, 1 hit PF12924, APP_Cu_bd, 1 hit PF12925, APP_E2, 1 hit PF02177, APP_N, 1 hit |
PRINTSi | PR00203, AMYLOIDA4 |
SMARTi | View protein in SMART SM00006, A4_EXTRA, 1 hit |
SUPFAMi | SSF109843, SSF109843, 1 hit SSF56491, SSF56491, 1 hit SSF89811, SSF89811, 1 hit |
PROSITEi | View protein in PROSITE PS00319, APP_CUBD, 1 hit PS51869, APP_E1, 1 hit PS51870, APP_E2, 1 hit PS00320, APP_INTRA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | APLP2_MOUSE | |
Accessioni | Q06335Primary (citable) accession number: Q06335 Secondary accession number(s): Q80US7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 28, 2011 | |
Last modified: | February 23, 2022 | |
This is version 174 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families