UniProtKB - Q06144 (ORM2_YEAST)
Protein
Protein ORM2
Gene
ORM2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Component of the SPOTS complex that acts as a negative regulator of sphingolipid synthesis. Acts by inhibiting serine palmitoyltransferases (LCB1 and LCB2) activity.1 Publication
Miscellaneous
Present with 5240 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- identical protein binding Source: IntAct
GO - Biological processi
- cellular sphingolipid homeostasis Source: UniProtKB
- ceramide metabolic process Source: GO_Central
- negative regulation of ceramide biosynthetic process Source: GO_Central
- negative regulation of sphingolipid biosynthetic process Source: UniProtKB
- response to unfolded protein Source: SGD
Enzyme and pathway databases
Reactomei | R-SCE-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: Protein ORM2 |
Gene namesi | Name:ORM2 Ordered Locus Names:YLR350W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YLR350W |
SGDi | S000004342, ORM2 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 2 Publications
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- SPOTS complex Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 78 | CytoplasmicSequence analysisAdd BLAST | 78 | |
Transmembranei | 79 – 99 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 100 – 103 | ExtracellularSequence analysis | 4 | |
Transmembranei | 104 – 124 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 125 – 148 | CytoplasmicSequence analysisAdd BLAST | 24 | |
Transmembranei | 149 – 169 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 170 – 177 | ExtracellularSequence analysis | 8 | |
Transmembranei | 178 – 198 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 199 – 216 | CytoplasmicSequence analysisAdd BLAST | 18 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 9 | S → A: Induces dysregulation of sphingolipid synthesis; when associated with A-15, A-18, A-36 and 46-A--A-48. 1 Publication | 1 | |
Mutagenesisi | 15 | S → A: Induces dysregulation of sphingolipid synthesis; when associated with A-9, A-18, A-36 and 46-A--A-48. 1 Publication | 1 | |
Mutagenesisi | 18 | T → A: Induces dysregulation of sphingolipid synthesis; when associated with A-9, A-15, A-36 and 46-A--A-48. 1 Publication | 1 | |
Mutagenesisi | 36 | T → A: Induces dysregulation of sphingolipid synthesis; when associated with A-9, A-15, A-18 and 46-A--A-48. 1 Publication | 1 | |
Mutagenesisi | 46 – 48 | SSS → AAA: Induces dysregulation of sphingolipid synthesis; when associated with A-9, A-15, A-18, and A-36. 1 Publication | 3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215646 | 1 – 216 | Protein ORM2Add BLAST | 216 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 9 | PhosphoserineCombined sources | 1 | |
Modified residuei | 15 | PhosphoserineCombined sources | 1 | |
Modified residuei | 18 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 22 | PhosphoserineCombined sources | 1 | |
Modified residuei | 29 | PhosphoserineBy similarity | 1 | |
Modified residuei | 51 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated in case of disruption of sphingolipid synthesis. Phosphorylation regulates inhibitory activity of serine palmitoyltransferases (LCB1 and LCB2).1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q06144 |
PaxDbi | Q06144 |
PRIDEi | Q06144 |
TopDownProteomicsi | Q06144 |
PTM databases
iPTMneti | Q06144 |
Interactioni
Subunit structurei
Component of the SPOTS complex, at least composed of LCB1/2 (LCB1 and/or LCB2), ORM1/2 (ORM1 and/or ORM2), SAC1 and TSC3.
1 PublicationBinary interactionsi
Hide detailsQ06144
With | #Exp. | IntAct |
---|---|---|
DFG5 [Q05031] | 3 | EBI-34916,EBI-27512 |
LCB1 [P25045] | 7 | EBI-34916,EBI-10059 |
ORM1 [P53224] | 6 | EBI-34916,EBI-12592 |
itself | 4 | EBI-34916,EBI-34916 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 31612, 554 interactors |
ComplexPortali | CPX-3158, SPOTS complex |
DIPi | DIP-5579N |
IntActi | Q06144, 45 interactors |
MINTi | Q06144 |
STRINGi | 4932.YLR350W |
Miscellaneous databases
RNActi | Q06144, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ORM family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3319, Eukaryota |
GeneTreei | ENSGT00950000183178 |
HOGENOMi | CLU_072117_2_1_1 |
InParanoidi | Q06144 |
OMAi | YKGAWII |
Family and domain databases
InterProi | View protein in InterPro IPR007203, ORMDL |
PANTHERi | PTHR12665, PTHR12665, 1 hit |
Pfami | View protein in Pfam PF04061, ORMDL, 1 hit |
PIRSFi | PIRSF018147, ORMDL, 1 hit |
i Sequence
Sequence statusi: Complete.
Q06144-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIDRTKNESP AFEESPLTPN VSNLKPFPSQ SNKISTPVTD HRRRRSSSVI
60 70 80 90 100
SHVEQETFED ENDQQMLPNM NATWVDQRGA WLIHIVVIVL LRLFYSLFGS
110 120 130 140 150
TPKWTWTLTN MTYIIGFYIM FHLVKGTPFD FNGGAYDNLT MWEQINDETL
160 170 180 190 200
YTPTRKFLLI VPIVLFLISN QYYRNDMTLF LSNLAVTVLI GVVPKLGITH
210
RLRISIPGIT GRAQIS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U19028 Genomic DNA Translation: AAB67252.1 AY692908 Genomic DNA Translation: AAT92927.1 BK006945 Genomic DNA Translation: DAA09654.1 |
PIRi | S51352 |
RefSeqi | NP_013454.1, NM_001182239.1 |
Genome annotation databases
EnsemblFungii | YLR350W_mRNA; YLR350W; YLR350W |
GeneIDi | 851064 |
KEGGi | sce:YLR350W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U19028 Genomic DNA Translation: AAB67252.1 AY692908 Genomic DNA Translation: AAT92927.1 BK006945 Genomic DNA Translation: DAA09654.1 |
PIRi | S51352 |
RefSeqi | NP_013454.1, NM_001182239.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 31612, 554 interactors |
ComplexPortali | CPX-3158, SPOTS complex |
DIPi | DIP-5579N |
IntActi | Q06144, 45 interactors |
MINTi | Q06144 |
STRINGi | 4932.YLR350W |
PTM databases
iPTMneti | Q06144 |
Proteomic databases
MaxQBi | Q06144 |
PaxDbi | Q06144 |
PRIDEi | Q06144 |
TopDownProteomicsi | Q06144 |
Genome annotation databases
EnsemblFungii | YLR350W_mRNA; YLR350W; YLR350W |
GeneIDi | 851064 |
KEGGi | sce:YLR350W |
Organism-specific databases
EuPathDBi | FungiDB:YLR350W |
SGDi | S000004342, ORM2 |
Phylogenomic databases
eggNOGi | KOG3319, Eukaryota |
GeneTreei | ENSGT00950000183178 |
HOGENOMi | CLU_072117_2_1_1 |
InParanoidi | Q06144 |
OMAi | YKGAWII |
Enzyme and pathway databases
Reactomei | R-SCE-6798695, Neutrophil degranulation |
Miscellaneous databases
PROi | PR:Q06144 |
RNActi | Q06144, protein |
Family and domain databases
InterProi | View protein in InterPro IPR007203, ORMDL |
PANTHERi | PTHR12665, PTHR12665, 1 hit |
Pfami | View protein in Pfam PF04061, ORMDL, 1 hit |
PIRSFi | PIRSF018147, ORMDL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ORM2_YEAST | |
Accessioni | Q06144Primary (citable) accession number: Q06144 Secondary accession number(s): D6VYY8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD