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UniProtKB - Q06142 (IMB1_YEAST)

Protein

Importin subunit beta-1

Gene

KAP95

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Importin beta subunit that functions in nuclear protein import through association with the importin alpha subunit, which binds to the classical nuclear localization signal (cNLS) in cargo substrates (PubMed:7622450). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by importin beta through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:8521485). At the nucleoplasmic side of the NPC, GTP-Ran binds to importin beta and the three components separate, leading to release of the cargo (PubMed:15864302). Importin alpha and beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin beta. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11423015). Mediates the nuclear import of histones H2A and H2B (PubMed:11309407).1 Publication4 Publications

Miscellaneous

Binds to nucleoporin FxFG but not GLFG repeat regions. Ran-GTP can disrupt the importin alpha/beta heterodimer by binding to the beta subunit and releases both subunits from the docking site.1 Publication
The stoichiometric complex between importin beta and Ran-GTP renders the latter inaccessible to Ran-specific GTPase activating protein (Ran-GAP) thereby inhibiting GTP hydrolysis stimulated by Ran-GAP.1 Publication
Present with 51700 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • disordered domain specific binding Source: CAFA
  • importin-alpha family protein binding Source: CAFA
  • nuclear localization sequence binding Source: GO_Central
  • protein-containing complex binding Source: SGD
  • protein transporter activity Source: SGD
  • Ran guanyl-nucleotide exchange factor activity Source: SGD
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32423-MONOMER
ReactomeiR-SCE-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-11 Publication
Alternative name(s):
Importin-95
Karyopherin subunit beta-11 Publication
Karyopherin-951 Publication
Gene namesi
Name:KAP951 Publication
Ordered Locus Names:YLR347CImported
ORF Names:L8300.15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

SGDiS000004339 KAP95

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001207642 – 861Importin subunit beta-1Add BLAST860

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei836PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ06142
PaxDbiQ06142
PRIDEiQ06142

PTM databases

iPTMnetiQ06142

Interactioni

Subunit structurei

Forms a complex with the importin alpha subunit (SRP1/KAP60) (PubMed:7622450). Interacts with Ran (GSP1); interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange (PubMed:8621381, PubMed:9321403). Interacts with nucleoporin NUP1 (PubMed:8521485, PubMed:15878174).5 Publications

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi31610, 615 interactors
ComplexPortaliCPX-1068 Importin complex, KAP60-KAP95
DIPiDIP-2357N
IntActiQ06142, 69 interactors
MINTiQ06142
STRINGi4932.YLR347C

Structurei

Secondary structure

1861
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ06142
SMRiQ06142
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ06142

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati3 – 35HEAT 11 PublicationAdd BLAST33
Domaini25 – 106Importin N-terminalPROSITE-ProRule annotationAdd BLAST82
Repeati37 – 66HEAT 21 PublicationAdd BLAST30
Repeati90 – 129HEAT 31 PublicationAdd BLAST40
Repeati134 – 164HEAT 41 PublicationAdd BLAST31
Repeati177 – 208HEAT 51 PublicationAdd BLAST32
Repeati219 – 255HEAT 61 PublicationAdd BLAST37
Repeati260 – 306HEAT 71 PublicationAdd BLAST47
Repeati317 – 362HEAT 81 PublicationAdd BLAST46
Repeati367 – 395HEAT 91 PublicationAdd BLAST29
Repeati402 – 442HEAT 101 PublicationAdd BLAST41
Repeati452 – 484HEAT 111 PublicationAdd BLAST33
Repeati496 – 530HEAT 121 PublicationAdd BLAST35
Repeati536 – 586HEAT 131 PublicationAdd BLAST51
Repeati592 – 629HEAT 141 PublicationAdd BLAST38
Repeati634 – 669HEAT 151 PublicationAdd BLAST36
Repeati675 – 713HEAT 161 PublicationAdd BLAST39
Repeati718 – 764HEAT 171 PublicationAdd BLAST47
Repeati773 – 812HEAT 181 PublicationAdd BLAST40
Repeati819 – 859HEAT 191 PublicationAdd BLAST41

Sequence similaritiesi

Belongs to the importin beta family. Importin beta-1 subfamily.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074898
HOGENOMiHOG000204108
InParanoidiQ06142
KOiK14293
OMAiKNHLTSK
OrthoDBiEOG092C12XO

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR000225 Armadillo
IPR021133 HEAT_type_2
IPR001494 Importin-beta_N
PfamiView protein in Pfam
PF03810 IBN_N, 1 hit
SMARTiView protein in SMART
SM00185 ARM, 2 hits
SM00913 IBN_N, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50077 HEAT_REPEAT, 1 hit
PS50166 IMPORTIN_B_NT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q06142-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTAEFAQLL ENSILSPDQN IRLTSETQLK KLSNDNFLQF AGLSSQVLID 
        60         70         80         90        100
ENTKLEGRIL AALTLKNELV SKDSVKTQQF AQRWITQVSP EAKNQIKTNA 
       110        120        130        140        150
LTALVSIEPR IANAAAQLIA AIADIELPHG AWPELMKIMV DNTGAEQPEN 
       160        170        180        190        200
VKRASLLALG YMCESADPQS QALVSSSNNI LIAIVQGAQS TETSKAVRLA 
       210        220        230        240        250
ALNALADSLI FIKNNMEREG ERNYLMQVVC EATQAEDIEV QAAAFGCLCK 
       260        270        280        290        300
IMSLYYTFMK PYMEQALYAL TIATMKSPND KVASMTVEFW STICEEEIDI 
       310        320        330        340        350
AYELAQFPQS PLQSYNFALS SIKDVVPNLL NLLTRQNEDP EDDDWNVSMS 
       360        370        380        390        400
AGACLQLFAQ NCGNHILEPV LEFVEQNITA DNWRNREAAV MAFGSIMDGP 
       410        420        430        440        450
DKVQRTYYVH QALPSILNLM NDQSLQVKET TAWCIGRIAD SVAESIDPQQ 
       460        470        480        490        500
HLPGVVQACL IGLQDHPKVA TNCSWTIINL VEQLAEATPS PIYNFYPALV 
       510        520        530        540        550
DGLIGAANRI DNEFNARASA FSALTTMVEY ATDTVAETSA SISTFVMDKL 
       560        570        580        590        600
GQTMSVDENQ LTLEDAQSLQ ELQSNILTVL AAVIRKSPSS VEPVADMLMG 
       610        620        630        640        650
LFFRLLEKKD SAFIEDDVFY AISALAASLG KGFEKYLETF SPYLLKALNQ 
       660        670        680        690        700
VDSPVSITAV GFIADISNSL EEDFRRYSDA MMNVLAQMIS NPNARRELKP 
       710        720        730        740        750
AVLSVFGDIA SNIGADFIPY LNDIMALCVA AQNTKPENGT LEALDYQIKV 
       760        770        780        790        800
LEAVLDAYVG IVAGLHDKPE ALFPYVGTIF QFIAQVAEDP QLYSEDATSR 
       810        820        830        840        850
AAVGLIGDIA AMFPDGSIKQ FYGQDWVIDY IKRTRSGQLF SQATKDTARW 
       860 
AREQQKRQLS L
Length:861
Mass (Da):94,776
Last modified:November 1, 1996 - v1
Checksum:iED530F5313E75B06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19028 Genomic DNA Translation: AAB67265.1
BK006945 Genomic DNA Translation: DAA09652.1
PIRiS51350
RefSeqiNP_013451.1, NM_001182236.1

Genome annotation databases

EnsemblFungiiYLR347C; YLR347C; YLR347C
GeneIDi851061
KEGGisce:YLR347C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19028 Genomic DNA Translation: AAB67265.1
BK006945 Genomic DNA Translation: DAA09652.1
PIRiS51350
RefSeqiNP_013451.1, NM_001182236.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BKUX-ray2.70B/D1-861[»]
3EA5X-ray2.50B/D1-861[»]
3ND2X-ray2.40A1-861[»]
5OWUX-ray2.00A1-861[»]
ProteinModelPortaliQ06142
SMRiQ06142
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31610, 615 interactors
ComplexPortaliCPX-1068 Importin complex, KAP60-KAP95
DIPiDIP-2357N
IntActiQ06142, 69 interactors
MINTiQ06142
STRINGi4932.YLR347C

PTM databases

iPTMnetiQ06142

Proteomic databases

MaxQBiQ06142
PaxDbiQ06142
PRIDEiQ06142

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR347C; YLR347C; YLR347C
GeneIDi851061
KEGGisce:YLR347C

Organism-specific databases

SGDiS000004339 KAP95

Phylogenomic databases

GeneTreeiENSGT00550000074898
HOGENOMiHOG000204108
InParanoidiQ06142
KOiK14293
OMAiKNHLTSK
OrthoDBiEOG092C12XO

Enzyme and pathway databases

BioCyciYEAST:G3O-32423-MONOMER
ReactomeiR-SCE-6798695 Neutrophil degranulation

Miscellaneous databases

EvolutionaryTraceiQ06142
PROiPR:Q06142

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR000225 Armadillo
IPR021133 HEAT_type_2
IPR001494 Importin-beta_N
PfamiView protein in Pfam
PF03810 IBN_N, 1 hit
SMARTiView protein in SMART
SM00185 ARM, 2 hits
SM00913 IBN_N, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50077 HEAT_REPEAT, 1 hit
PS50166 IMPORTIN_B_NT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIMB1_YEAST
AccessioniPrimary (citable) accession number: Q06142
Secondary accession number(s): D6VYY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 176 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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