UniProtKB - Q06142 (IMB1_YEAST)
Importin subunit beta-1
KAP95
Functioni
Importin beta subunit that functions in nuclear protein import through association with the importin alpha subunit, which binds to the classical nuclear localization signal (cNLS) in cargo substrates (PubMed:7622450).
Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by importin beta through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:8521485).
At the nucleoplasmic side of the NPC, GTP-Ran binds to importin beta and the three components separate, leading to release of the cargo (PubMed:15864302).
Importin alpha and beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin beta. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11423015).
Mediates the nuclear import of histones H2A and H2B (PubMed:11309407).
Mediates the nuclear import of transcription factor GCN4 (PubMed:14648200).
1 Publication5 PublicationsMiscellaneous
GO - Molecular functioni
- disordered domain specific binding Source: CAFA
- guanyl-nucleotide exchange factor activity Source: SGD
- importin-alpha family protein binding Source: CAFA
- nuclear import signal receptor activity Source: SGD
- nuclear localization sequence binding Source: GO_Central
- protein-containing complex binding Source: SGD
- small GTPase binding Source: InterPro
GO - Biological processi
- import into nucleus Source: ComplexPortal
- mRNA transport Source: UniProtKB-KW
- NLS-bearing protein import into nucleus Source: SGD
- nuclear pore complex assembly Source: SGD
- phosphatidylcholine biosynthetic process Source: SGD
- protein import into nucleus Source: UniProtKB
- protein targeting to membrane Source: SGD
- regulation of nucleocytoplasmic transport Source: SGD
- regulation of protein desumoylation Source: SGD
Keywordsi
Biological process | mRNA transport, Protein transport, Translocation, Transport |
Enzyme and pathway databases
Reactomei | R-SCE-2995383, Initiation of Nuclear Envelope (NE) Reformation R-SCE-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: Importin subunit beta-11 PublicationAlternative name(s): Importin-95Curated Karyopherin subunit beta-11 Publication Karyopherin-951 Publication |
Gene namesi | Ordered Locus Names:YLR347CImported ORF Names:L8300.15 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004339, KAP95 |
VEuPathDBi | FungiDB:YLR347C |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
- nuclear pore complex 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Cytosol
- cytosol Source: ComplexPortal
Nucleus
- nuclear envelope Source: ComplexPortal
- nuclear pore Source: UniProtKB-SubCell
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
- NLS-dependent protein nuclear import complex Source: SGD
Keywords - Cellular componenti
Cytoplasm, Nuclear pore complex, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000120764 | 2 – 861 | Importin subunit beta-1Add BLAST | 860 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 836 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | Q06142 |
PaxDbi | Q06142 |
PRIDEi | Q06142 |
PTM databases
iPTMneti | Q06142 |
Interactioni
Subunit structurei
Forms a complex with the importin alpha subunit (SRP1/KAP60) (PubMed:7622450).
Interacts with Ran (GSP1); interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange (PubMed:8621381, PubMed:9321403).
Interacts with nucleoporin NUP1 (PubMed:8521485, PubMed:15878174).
5 PublicationsBinary interactionsi
Q06142
GO - Molecular functioni
- disordered domain specific binding Source: CAFA
- importin-alpha family protein binding Source: CAFA
- small GTPase binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 31610, 623 interactors |
ComplexPortali | CPX-1068, Importin complex, KAP60-KAP95 |
DIPi | DIP-2357N |
IntActi | Q06142, 69 interactors |
MINTi | Q06142 |
STRINGi | 4932.YLR347C |
Miscellaneous databases
RNActi | Q06142, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q06142 |
SMRi | Q06142 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q06142 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 3 – 35 | HEAT 11 PublicationAdd BLAST | 33 | |
Domaini | 25 – 106 | Importin N-terminalPROSITE-ProRule annotationAdd BLAST | 82 | |
Repeati | 37 – 66 | HEAT 21 PublicationAdd BLAST | 30 | |
Repeati | 90 – 129 | HEAT 31 PublicationAdd BLAST | 40 | |
Repeati | 134 – 164 | HEAT 41 PublicationAdd BLAST | 31 | |
Repeati | 177 – 208 | HEAT 51 PublicationAdd BLAST | 32 | |
Repeati | 219 – 255 | HEAT 61 PublicationAdd BLAST | 37 | |
Repeati | 260 – 306 | HEAT 71 PublicationAdd BLAST | 47 | |
Repeati | 317 – 362 | HEAT 81 PublicationAdd BLAST | 46 | |
Repeati | 367 – 395 | HEAT 91 PublicationAdd BLAST | 29 | |
Repeati | 402 – 442 | HEAT 101 PublicationAdd BLAST | 41 | |
Repeati | 452 – 484 | HEAT 111 PublicationAdd BLAST | 33 | |
Repeati | 496 – 530 | HEAT 121 PublicationAdd BLAST | 35 | |
Repeati | 536 – 586 | HEAT 131 PublicationAdd BLAST | 51 | |
Repeati | 592 – 629 | HEAT 141 PublicationAdd BLAST | 38 | |
Repeati | 634 – 669 | HEAT 151 PublicationAdd BLAST | 36 | |
Repeati | 675 – 713 | HEAT 161 PublicationAdd BLAST | 39 | |
Repeati | 718 – 764 | HEAT 171 PublicationAdd BLAST | 47 | |
Repeati | 773 – 812 | HEAT 181 PublicationAdd BLAST | 40 | |
Repeati | 819 – 859 | HEAT 191 PublicationAdd BLAST | 41 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1241, Eukaryota |
GeneTreei | ENSGT00550000074898 |
HOGENOMi | CLU_008296_1_0_1 |
InParanoidi | Q06142 |
OMAi | GRICDII |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR000225, Armadillo IPR021133, HEAT_type_2 IPR001494, Importin-beta_N IPR040122, Importin_beta |
PANTHERi | PTHR10527, PTHR10527, 1 hit |
Pfami | View protein in Pfam PF03810, IBN_N, 1 hit |
SMARTi | View protein in SMART SM00185, ARM, 2 hits SM00913, IBN_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50077, HEAT_REPEAT, 1 hit PS50166, IMPORTIN_B_NT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSTAEFAQLL ENSILSPDQN IRLTSETQLK KLSNDNFLQF AGLSSQVLID
60 70 80 90 100
ENTKLEGRIL AALTLKNELV SKDSVKTQQF AQRWITQVSP EAKNQIKTNA
110 120 130 140 150
LTALVSIEPR IANAAAQLIA AIADIELPHG AWPELMKIMV DNTGAEQPEN
160 170 180 190 200
VKRASLLALG YMCESADPQS QALVSSSNNI LIAIVQGAQS TETSKAVRLA
210 220 230 240 250
ALNALADSLI FIKNNMEREG ERNYLMQVVC EATQAEDIEV QAAAFGCLCK
260 270 280 290 300
IMSLYYTFMK PYMEQALYAL TIATMKSPND KVASMTVEFW STICEEEIDI
310 320 330 340 350
AYELAQFPQS PLQSYNFALS SIKDVVPNLL NLLTRQNEDP EDDDWNVSMS
360 370 380 390 400
AGACLQLFAQ NCGNHILEPV LEFVEQNITA DNWRNREAAV MAFGSIMDGP
410 420 430 440 450
DKVQRTYYVH QALPSILNLM NDQSLQVKET TAWCIGRIAD SVAESIDPQQ
460 470 480 490 500
HLPGVVQACL IGLQDHPKVA TNCSWTIINL VEQLAEATPS PIYNFYPALV
510 520 530 540 550
DGLIGAANRI DNEFNARASA FSALTTMVEY ATDTVAETSA SISTFVMDKL
560 570 580 590 600
GQTMSVDENQ LTLEDAQSLQ ELQSNILTVL AAVIRKSPSS VEPVADMLMG
610 620 630 640 650
LFFRLLEKKD SAFIEDDVFY AISALAASLG KGFEKYLETF SPYLLKALNQ
660 670 680 690 700
VDSPVSITAV GFIADISNSL EEDFRRYSDA MMNVLAQMIS NPNARRELKP
710 720 730 740 750
AVLSVFGDIA SNIGADFIPY LNDIMALCVA AQNTKPENGT LEALDYQIKV
760 770 780 790 800
LEAVLDAYVG IVAGLHDKPE ALFPYVGTIF QFIAQVAEDP QLYSEDATSR
810 820 830 840 850
AAVGLIGDIA AMFPDGSIKQ FYGQDWVIDY IKRTRSGQLF SQATKDTARW
860
AREQQKRQLS L
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U19028 Genomic DNA Translation: AAB67265.1 BK006945 Genomic DNA Translation: DAA09652.1 |
PIRi | S51350 |
RefSeqi | NP_013451.1, NM_001182236.1 |
Genome annotation databases
EnsemblFungii | YLR347C_mRNA; YLR347C; YLR347C |
GeneIDi | 851061 |
KEGGi | sce:YLR347C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U19028 Genomic DNA Translation: AAB67265.1 BK006945 Genomic DNA Translation: DAA09652.1 |
PIRi | S51350 |
RefSeqi | NP_013451.1, NM_001182236.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2BKU | X-ray | 2.70 | B/D | 1-861 | [»] | |
3EA5 | X-ray | 2.50 | B/D | 1-861 | [»] | |
3ND2 | X-ray | 2.40 | A | 1-861 | [»] | |
5OWU | X-ray | 2.00 | A | 1-861 | [»] | |
AlphaFoldDBi | Q06142 | |||||
SMRi | Q06142 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31610, 623 interactors |
ComplexPortali | CPX-1068, Importin complex, KAP60-KAP95 |
DIPi | DIP-2357N |
IntActi | Q06142, 69 interactors |
MINTi | Q06142 |
STRINGi | 4932.YLR347C |
PTM databases
iPTMneti | Q06142 |
Proteomic databases
MaxQBi | Q06142 |
PaxDbi | Q06142 |
PRIDEi | Q06142 |
Genome annotation databases
EnsemblFungii | YLR347C_mRNA; YLR347C; YLR347C |
GeneIDi | 851061 |
KEGGi | sce:YLR347C |
Organism-specific databases
SGDi | S000004339, KAP95 |
VEuPathDBi | FungiDB:YLR347C |
Phylogenomic databases
eggNOGi | KOG1241, Eukaryota |
GeneTreei | ENSGT00550000074898 |
HOGENOMi | CLU_008296_1_0_1 |
InParanoidi | Q06142 |
OMAi | GRICDII |
Enzyme and pathway databases
Reactomei | R-SCE-2995383, Initiation of Nuclear Envelope (NE) Reformation R-SCE-6798695, Neutrophil degranulation |
Miscellaneous databases
EvolutionaryTracei | Q06142 |
PROi | PR:Q06142 |
RNActi | Q06142, protein |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR000225, Armadillo IPR021133, HEAT_type_2 IPR001494, Importin-beta_N IPR040122, Importin_beta |
PANTHERi | PTHR10527, PTHR10527, 1 hit |
Pfami | View protein in Pfam PF03810, IBN_N, 1 hit |
SMARTi | View protein in SMART SM00185, ARM, 2 hits SM00913, IBN_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50077, HEAT_REPEAT, 1 hit PS50166, IMPORTIN_B_NT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IMB1_YEAST | |
Accessioni | Q06142Primary (citable) accession number: Q06142 Secondary accession number(s): D6VYY6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 196 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families