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Entry version 177 (10 Feb 2021)
Sequence version 2 (19 Jul 2004)
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Protein

A-factor-processing enzyme

Gene

STE23

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the N-terminal endoproteolytic cleavage of the P2 precursor of the a-factor mating pheromone. Capable of proteolysing the established mammalian insulin-degrading enzymes (IDEs) substrates amyloid-beta peptide and insulin B-chain.

3 Publications

Caution

It is uncertain whether Met-1 or Met-53 is the initiator. Met-53 is probably the physiologically relevant initiator methionine, as Met-1 is dispensable for the expression of functional STE23, whereas Met-53 is not.Curated

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by chelating agents like EDTA, TPEN and 1,1-phenanthroline, as well as NEM, free cysteine and DTT.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=215 µM for a synthetic a-factor analog1 Publication
  1. Vmax=0.49 µmol/min/mg enzyme towards a synthetic a-factor analog1 Publication

pH dependencei

Optimum pH is 8.1.1 Publication

Temperature dependencei

Optimum temperature is 37 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi118ZincPROSITE-ProRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei121Proton acceptorPROSITE-ProRule annotation1
Metal bindingi122ZincPROSITE-ProRule annotation1
Metal bindingi199ZincPROSITE-ProRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: SGD

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processPheromone response
LigandMetal-binding, Zinc

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-SCE-9033241, Peroxisomal protein import

Protein family/group databases

MEROPS protease database

More...
MEROPSi
M16.008

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
A-factor-processing enzyme (EC:3.4.24.-)
Alternative name(s):
Insulin-degrading enzyme homolog
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:STE23
Ordered Locus Names:YLR389C
ORF Names:L8084.12
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XII

Organism-specific databases

Saccharomyces Genome Database

More...
SGDi
S000004381, STE23

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
FungiDB:YLR389C

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000744181 – 1027A-factor-processing enzymeAdd BLAST1027

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q06010

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q06010

PRoteomics IDEntifications database

More...
PRIDEi
Q06010

Consortium for Top Down Proteomics

More...
TopDownProteomicsi
Q06010

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q06010

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Expressed in both haploid and diploid yeast cells.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
31648, 62 interactors

Database of interacting proteins

More...
DIPi
DIP-1921N

Protein interaction database and analysis system

More...
IntActi
Q06010, 7 interactors

Molecular INTeraction database

More...
MINTi
Q06010

STRING: functional protein association networks

More...
STRINGi
4932.YLR389C

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
Q06010, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q06010

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the peptidase M16 family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG0959, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000155780

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_004639_1_1_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q06010

Identification of Orthologs from Complete Genome Data

More...
OMAi
HGNLYKE

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011249, Metalloenz_LuxS/M16
IPR011765, Pept_M16_N
IPR001431, Pept_M16_Zn_BS
IPR007863, Peptidase_M16_C
IPR032632, Peptidase_M16_M

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00675, Peptidase_M16, 1 hit
PF05193, Peptidase_M16_C, 2 hits
PF16187, Peptidase_M16_M, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF63411, SSF63411, 4 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00143, INSULINASE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q06010-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGVSLLASSS AFVTKPLLTQ LVHLSPISLN FTVRRFKPFT CLSRYYTTNP
60 70 80 90 100
YNMTSNFKTF NLDFLKPDLD ERSYRFIELP NKLKALLIQD PKADKAAASL
110 120 130 140 150
DVNIGAFEDP KNLPGLAHFC EHLLFMGSEK FPDENEYSSY LSKHGGSSNA
160 170 180 190 200
YTASQNTNYF FEVNHQHLFG ALDRFSGFFS CPLFNKDSTD KEINAVNSEN
210 220 230 240 250
KKNLQNDIWR IYQLDKSLTN TKHPYHKFST GNIETLGTLP KENGLNVRDE
260 270 280 290 300
LLKFHKNFYS ANLMKLCILG REDLDTLSDW TYDLFKDVAN NGREVPLYAE
310 320 330 340 350
PIMQPEHLQK IIQVRPVKDL KKLEISFTVP DMEEHWESKP PRILSHLIGH
360 370 380 390 400
EGSGSLLAHL KKLGWANELS AGGHTVSKGN AFFAVDIDLT DNGLTHYRDV
410 420 430 440 450
IVLIFQYIEM LKNSLPQKWI FNELQDISNA TFKFKQAGSP SSTVSSLAKC
460 470 480 490 500
LEKDYIPVSR ILAMGLLTKY EPDLLTQYTD ALVPENSRVT LISRSLETDS
510 520 530 540 550
AEKWYGTAYK VVDYPADLIK NMKSPGLNPA LTLPRPNEFV STNFKVDKID
560 570 580 590 600
GIKPLDEPVL LLSDDVSKLW YKKDDRFWQP RGYIYLSFKL PHTHASIINS
610 620 630 640 650
MLSTLYTQLA NDALKDVQYD AACADLRISF NKTNQGLAIT ASGFNEKLII
660 670 680 690 700
LLTRFLQGVN SFEPKKDRFE ILKDKTIRHL KNLLYEVPYS QMSNYYNAII
710 720 730 740 750
NERSWSTAEK LQVFEKLTFE QLINFIPTIY EGVYFETLIH GNIKHEEALE
760 770 780 790 800
VDSLIKSLIP NNIHNLQVSN NRLRSYLLPK GKTFRYETAL KDSQNVNSCI
810 820 830 840 850
QHVTQLDVYS EDLSALSGLF AQLIHEPCFD TLRTKEQLGY VVFSSSLNNH
860 870 880 890 900
GTANIRILIQ SEHTTPYLEW RINNFYETFG QVLRDMPEED FEKHKEALCN
910 920 930 940 950
SLLQKFKNMA EESARYTAAI YLGDYNFTHR QKKAKLVANI TKQQMIDFYE
960 970 980 990 1000
NYIMSENASK LILHLKSQVE NKELNENELD TAKYPTGQLI EDVGAFKSTL
1010 1020
FVAPVRQPMK DFEISAPPKL NNSSESE
Length:1,027
Mass (Da):117,579
Last modified:July 19, 2004 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC07B7F7EDA5785F3
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U19729 Genomic DNA Translation: AAB82351.2
AY260885 Genomic DNA Translation: AAP21753.1
BK006945 Genomic DNA Translation: DAA09690.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S55945

NCBI Reference Sequences

More...
RefSeqi
NP_013493.2, NM_001182278.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YLR389C_mRNA; YLR389C; YLR389C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
851105

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YLR389C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19729 Genomic DNA Translation: AAB82351.2
AY260885 Genomic DNA Translation: AAP21753.1
BK006945 Genomic DNA Translation: DAA09690.1
PIRiS55945
RefSeqiNP_013493.2, NM_001182278.1

3D structure databases

SMRiQ06010
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi31648, 62 interactors
DIPiDIP-1921N
IntActiQ06010, 7 interactors
MINTiQ06010
STRINGi4932.YLR389C

Protein family/group databases

MEROPSiM16.008

PTM databases

iPTMnetiQ06010

Proteomic databases

MaxQBiQ06010
PaxDbiQ06010
PRIDEiQ06010
TopDownProteomicsiQ06010

Genome annotation databases

EnsemblFungiiYLR389C_mRNA; YLR389C; YLR389C
GeneIDi851105
KEGGisce:YLR389C

Organism-specific databases

SGDiS000004381, STE23
VEuPathDBiFungiDB:YLR389C

Phylogenomic databases

eggNOGiKOG0959, Eukaryota
GeneTreeiENSGT00940000155780
HOGENOMiCLU_004639_1_1_1
InParanoidiQ06010
OMAiHGNLYKE

Enzyme and pathway databases

ReactomeiR-SCE-9033241, Peroxisomal protein import

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q06010
RNActiQ06010, protein

Family and domain databases

InterProiView protein in InterPro
IPR011249, Metalloenz_LuxS/M16
IPR011765, Pept_M16_N
IPR001431, Pept_M16_Zn_BS
IPR007863, Peptidase_M16_C
IPR032632, Peptidase_M16_M
PfamiView protein in Pfam
PF00675, Peptidase_M16, 1 hit
PF05193, Peptidase_M16_C, 2 hits
PF16187, Peptidase_M16_M, 1 hit
SUPFAMiSSF63411, SSF63411, 4 hits
PROSITEiView protein in PROSITE
PS00143, INSULINASE, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSTE23_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q06010
Secondary accession number(s): D6VZ24, E9P8R3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 19, 2004
Last modified: February 10, 2021
This is version 177 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families
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