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Protein

Eukaryotic translation initiation factor 5B

Gene

Eif5b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.By similarity

Catalytic activityi

GTP + H2O = GDP + phosphate.By similarity

Cofactori

a monovalent cationBy similarityNote: Binds 1 monovalent cation per monomer in the active site. Structural cofactor that stabilizes the GTP-bound "on" state. May also act as a transition state stabilizer of the hydrolysis reaction.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi634 – 641GTPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Initiation factor
Biological processProtein biosynthesis
LigandGTP-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5B (EC:3.6.5.3)
Short name:
eIF-5B
Alternative name(s):
Translation initiation factor IF-2
Gene namesi
Name:Eif5b
Synonyms:If2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2441772 Eif5b

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003540711 – 1216Eukaryotic translation initiation factor 5BAdd BLAST1216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei66PhosphoserineBy similarity1
Modified residuei107PhosphothreonineCombined sources1
Modified residuei108PhosphoserineCombined sources1
Modified residuei114PhosphoserineCombined sources1
Modified residuei137PhosphoserineCombined sources1
Modified residuei139PhosphoserineCombined sources1
Modified residuei145PhosphoserineCombined sources1
Modified residuei165PhosphoserineCombined sources1
Modified residuei172PhosphoserineBy similarity1
Modified residuei183PhosphoserineCombined sources1
Modified residuei184PhosphoserineCombined sources1
Modified residuei187PhosphoserineCombined sources1
Modified residuei191PhosphoserineCombined sources1
Modified residuei209PhosphoserineCombined sources1
Modified residuei215PhosphoserineCombined sources1
Modified residuei223PhosphoserineCombined sources1
Modified residuei300PhosphothreonineBy similarity1
Modified residuei437PhosphoserineBy similarity1
Modified residuei497PhosphothreonineBy similarity1
Modified residuei544PhosphoserineBy similarity1
Modified residuei553PhosphoserineBy similarity1
Modified residuei556PhosphoserineBy similarity1
Modified residuei584PhosphoserineBy similarity1
Modified residuei585PhosphoserineBy similarity1
Modified residuei587PhosphoserineBy similarity1
Modified residuei591PhosphoserineBy similarity1
Modified residuei1164PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ05D44
MaxQBiQ05D44
PaxDbiQ05D44
PeptideAtlasiQ05D44
PRIDEiQ05D44

PTM databases

iPTMnetiQ05D44
PhosphoSitePlusiQ05D44
SwissPalmiQ05D44

Expressioni

Gene expression databases

BgeeiENSMUSG00000026083 Expressed in 295 organ(s), highest expression level in lens of camera-type eye
GenevisibleiQ05D44 MM

Interactioni

Subunit structurei

Interacts with ANXA5 in a calcium and phospholipid-dependent manner.By similarity

Protein-protein interaction databases

BioGridi230580, 2 interactors
STRINGi10090.ENSMUSP00000027252

Structurei

3D structure databases

ProteinModelPortaliQ05D44
SMRiQ05D44
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini625 – 842tr-type GPROSITE-ProRule annotationAdd BLAST218

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni634 – 641G1PROSITE-ProRule annotation8
Regioni659 – 663G2PROSITE-ProRule annotation5
Regioni698 – 701G3PROSITE-ProRule annotation4
Regioni752 – 755G4PROSITE-ProRule annotation4
Regioni820 – 822G5PROSITE-ProRule annotation3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi39 – 452Lys-richAdd BLAST414
Compositional biasi235 – 561Glu-richAdd BLAST327

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1144 Eukaryota
COG0532 LUCA
GeneTreeiENSGT00900000141098
HOVERGENiHBG019036
InParanoidiQ05D44
KOiK03243
OMAiRHTNVQD
OrthoDBiEOG091G114V
PhylomeDBiQ05D44
TreeFamiTF101535

Family and domain databases

Gene3Di3.40.50.10050, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR015760 TIF_IF2
IPR023115 TIF_IF2_dom3
IPR036925 TIF_IF2_dom3_sf
IPR009000 Transl_B-barrel_sf
PANTHERiPTHR43381 PTHR43381, 1 hit
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF11987 IF-2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52156 SSF52156, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q05D44-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGKKQKNKSE DSTKDDTDLG ALAAEIEGAG AAKEQEPQKS KGKKKKEKKK
60 70 80 90 100
QDFDENDILR ELEELSLEAQ GIRADRDAAA VKPTENNEEE SASKQDKKKK
110 120 130 140 150
GQKGKKTSFD ENDSEELEDK DSKSKKTARP NSEAPLSGSE DADDSNKLSK
160 170 180 190 200
KGKKAQKSTK KRDGSEEDED NSKRSKERSR VNSSGESGGE SDEFLQSRKG
210 220 230 240 250
QKKNQKNKSV PTVDSGNEDD DSSFKIKTVA QKKAEKKERE KKKRDEEKAK
260 270 280 290 300
LRKMKEKEEL EKGKKEQSKQ REPQKRPEEE VLTLRGTPDT GAASEEKGDT
310 320 330 340 350
AAALEDDNEG DKKKKDKKKK KTEKDEKEKE KKKGPSKSTV KAIQEALAKL
360 370 380 390 400
KEEEERQKRE EEERIKRLEE LEAKRKEEER LEQEKRERKK QKEKERKERL
410 420 430 440 450
KKEGKLLTKS QREARARAEV TLRHLQAQGV EVPSKDSLPK KRPVYEDKKK
460 470 480 490 500
KKTPQQLESK EVSETLEISA PVEAVDQGGP EKEETPPSVE PEEEEDTEDA
510 520 530 540 550
GLDDWEAMAS DEEREKEGNM IHIEVEENPE EEEEEEEEEE EEESEDEEEE
560 570 580 590 600
GDSEGSDGDE EDCKLSDEKD SGKAGDTKPS KDASSDSEYD SDDDRTKEER
610 620 630 640 650
AYDKAKRRIE KRRLEHGKNV NTEKLRAPII CVLGHVDTGK TKILDKLRHT
660 670 680 690 700
HVQDGEAGGI TQQIGATNVP LEAINEQTKM IKNFDRENVR IPGMLIIDTP
710 720 730 740 750
GHESFSNLRN RGSSLCDIAI LVVDIMHGLE PQTIESINIL KSKKCPFIVA
760 770 780 790 800
LNKIDRLYDW KKSPDSDVAV TLKKQKKNTK DEFEERAKAI IVEFAQQGLN
810 820 830 840 850
AALFYENKDP RTFVSLVPTS AHTGDGMGSL IYLLVELTQT MLSKRLAHCE
860 870 880 890 900
ELRAQVMEVK ALPGMGTTID VILINGRLKE GDTIIVPGVE GPIVTQIRGL
910 920 930 940 950
LLPPPMKELR VKNQYEKHKE VEAAQGVKIL GKDLEKTLAG LPLLVAYKDD
960 970 980 990 1000
EIPVLKDELI HELKQTLNAI KLEEKGVYVQ ASTLGSLEAL LEFLKTSEVP
1010 1020 1030 1040 1050
YAGINIGPVH KKDVMKASVM LEHDPQYAVI LAFDVRIERD AQEMADSLGV
1060 1070 1080 1090 1100
RIFSAEIIYH LFDAFTKYRQ DYKKQKQEEF KHIAVFPCKM KILPQYIFNS
1110 1120 1130 1140 1150
RDPIVIGVTV EAGQVKQGTP MCVPSKNFVD IGIVTSIEIN HKQVDVAKKG
1160 1170 1180 1190 1200
QEVCVKIEPI PGESPKMFGR HFEATDILVS KISRQSIDAL KDWFRDEMQK
1210
SDWQLIVELK KVFEII
Length:1,216
Mass (Da):137,616
Last modified:November 25, 2008 - v2
Checksum:i38D1C21648E4EAEC
GO

Sequence cautioni

The sequence AAH18347 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH37150 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH40746 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH60288 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121D → N in BAE26259 (PubMed:16141072).Curated1
Sequence conflicti188G → C in BAE26259 (PubMed:16141072).Curated1
Sequence conflicti254M → L in BAE26259 (PubMed:16141072).Curated1
Sequence conflicti302A → V in BAE26259 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC114583 Genomic DNA No translation available.
AK145146 mRNA Translation: BAE26259.1
AK145732 mRNA Translation: BAE26614.1
AK163527 mRNA Translation: BAE37384.1
BC018347 mRNA Translation: AAH18347.1 Sequence problems.
BC037150 mRNA Translation: AAH37150.1 Sequence problems.
BC040746 mRNA Translation: AAH40746.1 Sequence problems.
BC060288 mRNA Translation: AAH60288.1 Sequence problems.
CCDSiCCDS35544.1
RefSeqiNP_938045.2, NM_198303.2
UniGeneiMm.260943

Genome annotation databases

EnsembliENSMUST00000027252; ENSMUSP00000027252; ENSMUSG00000026083
GeneIDi226982
KEGGimmu:226982
UCSCiuc007aso.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC114583 Genomic DNA No translation available.
AK145146 mRNA Translation: BAE26259.1
AK145732 mRNA Translation: BAE26614.1
AK163527 mRNA Translation: BAE37384.1
BC018347 mRNA Translation: AAH18347.1 Sequence problems.
BC037150 mRNA Translation: AAH37150.1 Sequence problems.
BC040746 mRNA Translation: AAH40746.1 Sequence problems.
BC060288 mRNA Translation: AAH60288.1 Sequence problems.
CCDSiCCDS35544.1
RefSeqiNP_938045.2, NM_198303.2
UniGeneiMm.260943

3D structure databases

ProteinModelPortaliQ05D44
SMRiQ05D44
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230580, 2 interactors
STRINGi10090.ENSMUSP00000027252

PTM databases

iPTMnetiQ05D44
PhosphoSitePlusiQ05D44
SwissPalmiQ05D44

Proteomic databases

EPDiQ05D44
MaxQBiQ05D44
PaxDbiQ05D44
PeptideAtlasiQ05D44
PRIDEiQ05D44

Protocols and materials databases

DNASUi226982
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027252; ENSMUSP00000027252; ENSMUSG00000026083
GeneIDi226982
KEGGimmu:226982
UCSCiuc007aso.1 mouse

Organism-specific databases

CTDi9669
MGIiMGI:2441772 Eif5b

Phylogenomic databases

eggNOGiKOG1144 Eukaryota
COG0532 LUCA
GeneTreeiENSGT00900000141098
HOVERGENiHBG019036
InParanoidiQ05D44
KOiK03243
OMAiRHTNVQD
OrthoDBiEOG091G114V
PhylomeDBiQ05D44
TreeFamiTF101535

Miscellaneous databases

ChiTaRSiEif5b mouse
PROiPR:Q05D44
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026083 Expressed in 295 organ(s), highest expression level in lens of camera-type eye
GenevisibleiQ05D44 MM

Family and domain databases

Gene3Di3.40.50.10050, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR015760 TIF_IF2
IPR023115 TIF_IF2_dom3
IPR036925 TIF_IF2_dom3_sf
IPR009000 Transl_B-barrel_sf
PANTHERiPTHR43381 PTHR43381, 1 hit
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF11987 IF-2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52156 SSF52156, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51722 G_TR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIF2P_MOUSE
AccessioniPrimary (citable) accession number: Q05D44
Secondary accession number(s): Q3SYI4
, Q3TQJ8, Q3UL37, Q3UM39, Q6PAI0, Q8CFF4, Q8CGD6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: November 7, 2018
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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