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Protein

Zona pellucida sperm-binding protein 2

Gene

ZP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.By similarity

GO - Molecular functioni

  • acrosin binding Source: UniProtKB
  • coreceptor activity Source: ProtInc
  • identical protein binding Source: IntAct

GO - Biological processi

  • binding of sperm to zona pellucida Source: UniProtKB
  • prevention of polyspermy Source: UniProtKB

Keywordsi

Molecular functionReceptor
Biological processFertilization

Enzyme and pathway databases

ReactomeiR-HSA-1300644 Interaction With The Zona Pellucida

Names & Taxonomyi

Protein namesi
Recommended name:
Zona pellucida sperm-binding protein 2
Alternative name(s):
Zona pellucida glycoprotein 2
Short name:
Zp-2
Zona pellucida protein A
Cleaved into the following chain:
Gene namesi
Name:ZP2
Synonyms:ZPA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000103310.10
HGNCiHGNC:13188 ZP2
MIMi182888 gene
neXtProtiNX_Q05996

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini39 – 716ExtracellularSequence analysisAdd BLAST678
Transmembranei717 – 736HelicalSequence analysisAdd BLAST20
Topological domaini737 – 745CytoplasmicSequence analysis9

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

MalaCardsiZP2
OpenTargetsiENSG00000103310
Orphaneti404466 Female infertility due to zona pellucida defect
PharmGKBiPA37756

Polymorphism and mutation databases

BioMutaiZP2
DMDMi466206

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 38Add BLAST38
ChainiPRO_000004168939 – 640Zona pellucida sperm-binding protein 2Add BLAST602
ChainiPRO_000030455939 – ?Processed zona pellucida sperm-binding protein 2
PropeptideiPRO_0000041690641 – 745Removed in mature formBy similarityAdd BLAST105

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi55 ↔ 138By similarity
Disulfide bondi88 ↔ 106By similarity
Glycosylationi105N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi122N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi372 ↔ 465By similarity
Disulfide bondi403 ↔ 424By similarity
Glycosylationi462O-linked (GalNAc...) threonineBy similarity1
Disulfide bondi545 ↔ 615By similarity
Disulfide bondi566 ↔ 634By similarity
Disulfide bondi620 ↔ 630By similarity

Post-translational modificationi

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.By similarity
Proteolytically cleaved in the N-terminal part by the metalloendopeptidase ASTL exocytosed from cortical granules after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy. Additional proteolytically cleavage of the N-terminal peptide of 30 kDa occurs in one-cell and two-cell embryos.By similarity
N-glycosylated.By similarity
O-glycosylated; contains sulfate-substituted glycans.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei172 – 173Cleavage; by ASTLBy similarity2
Sitei640 – 641CleavageBy similarity2

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ05996
PaxDbiQ05996
PeptideAtlasiQ05996
PRIDEiQ05996
ProteomicsDBi58364

PTM databases

iPTMnetiQ05996
PhosphoSitePlusiQ05996

Expressioni

Gene expression databases

BgeeiENSG00000103310 Expressed in 43 organ(s), highest expression level in oocyte
CleanExiHS_ZP2
GenevisibleiQ05996 HS

Organism-specific databases

HPAiHPA011296

Interactioni

Subunit structurei

Can form homopolymers that assemble into long fibers (in vitro). Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers (By similarity). Interacts with ZP3 (PubMed:28886344).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-1755919,EBI-1755919

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113564, 8 interactors
IntActiQ05996, 2 interactors
MINTiQ05996
STRINGi9606.ENSP00000219593

Structurei

3D structure databases

ProteinModelPortaliQ05996
SMRiQ05996
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini371 – 637ZPPROSITE-ProRule annotationAdd BLAST267

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni469 – 491DisorderedBy similarityAdd BLAST23

Domaini

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.By similarity

Sequence similaritiesi

Belongs to the ZP domain family. ZPA subfamily.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHBM Eukaryota
ENOG4110GZY LUCA
GeneTreeiENSGT00530000063096
HOGENOMiHOG000059563
HOVERGENiHBG004379
InParanoidiQ05996
KOiK19927
OMAiEFPGKLK
OrthoDBiEOG091G02TE
PhylomeDBiQ05996
TreeFamiTF332794

Family and domain databases

InterProiView protein in InterPro
IPR001507 ZP_dom
IPR017977 ZP_dom_CS
PfamiView protein in Pfam
PF00100 Zona_pellucida, 1 hit
PRINTSiPR00023 ZPELLUCIDA
SMARTiView protein in SMART
SM00241 ZP, 1 hit
PROSITEiView protein in PROSITE
PS00682 ZP_1, 1 hit
PS51034 ZP_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q05996-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MACRQRGGSW SPSGWFNAGW STYRSISLFF ALVTSGNSID VSQLVNPAFP
60 70 80 90 100
GTVTCDEREI TVEFPSSPGT KKWHASVVDP LGLDMPNCTY ILDPEKLTLR
110 120 130 140 150
ATYDNCTRRV HGGHQMTIRV MNNSAALRHG AVMYQFFCPA MQVEETQGLS
160 170 180 190 200
ASTICQKDFM SFSLPRVFSG LADDSKGTKV QMGWSIEVGD GARAKTLTLP
210 220 230 240 250
EAMKEGFSLL IDNHRMTFHV PFNATGVTHY VQGNSHLYMV SLKLTFISPG
260 270 280 290 300
QKVIFSSQAI CAPDPVTCNA THMTLTIPEF PGKLKSVSFE NQNIDVSQLH
310 320 330 340 350
DNGIDLEATN GMKLHFSKTL LKTKLSEKCL LHQFYLASLK LTFLLRPETV
360 370 380 390 400
SMVIYPECLC ESPVSIVTGE LCTQDGFMDV EVYSYQTQPA LDLGTLRVGN
410 420 430 440 450
SSCQPVFEAQ SQGLVRFHIP LNGCGTRYKF EDDKVVYENE IHALWTDFPP
460 470 480 490 500
SKISRDSEFR MTVKCSYSRN DMLLNINVES LTPPVASVKL GPFTLILQSY
510 520 530 540 550
PDNSYQQPYG ENEYPLVRFL RQPIYMEVRV LNRDDPNIKL VLDDCWATST
560 570 580 590 600
MDPDSFPQWN VVVDGCAYDL DNYQTTFHPV GSSVTHPDHY QRFDMKAFAF
610 620 630 640 650
VSEAHVLSSL VYFHCSALIC NRLSPDSPLC SVTCPVSSRH RRATGATEAE
660 670 680 690 700
KMTVSLPGPI LLLSDDSSFR GVGSSDLKAS GSSGEKSRSE TGEEVGSRGA
710 720 730 740
MDTKGHKTAG DVGSKAVAAV AAFAGVVATL GFIYYLYEKR TVSNH
Length:745
Mass (Da):82,357
Last modified:February 1, 1994 - v1
Checksum:i2614DA79738F2CDD
GO
Isoform 2 (identifier: Q05996-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     460-468: Missing.

Note: No experimental confirmation available.
Show »
Length:736
Mass (Da):81,300
Checksum:i0FA63E0D52271077
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02470536G → V1 PublicationCorresponds to variant dbSNP:rs2075520Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054508460 – 468Missing in isoform 2. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90366 mRNA Translation: AAA61335.1
AK313003 mRNA Translation: BAG35839.1
AF001550 Genomic DNA Translation: AAB67599.1
CH471228 Genomic DNA Translation: EAW66859.1
BC096304 mRNA Translation: AAH96304.1
BC096305 mRNA Translation: AAH96305.1
BC096306 mRNA Translation: AAH96306.1
BC096307 mRNA Translation: AAH96307.1
CCDSiCCDS10596.1 [Q05996-1]
CCDS73843.1 [Q05996-2]
PIRiA48833
RefSeqiNP_001277033.1, NM_001290104.1 [Q05996-2]
NP_003451.1, NM_003460.2 [Q05996-1]
UniGeneiHs.73982

Genome annotation databases

EnsembliENST00000574002; ENSP00000460971; ENSG00000103310 [Q05996-1]
ENST00000574091; ENSP00000458991; ENSG00000103310 [Q05996-2]
ENST00000638300; ENSP00000492703; ENSG00000284588 [Q05996-1]
ENST00000640487; ENSP00000491583; ENSG00000284588 [Q05996-2]
GeneIDi7783
KEGGihsa:7783
UCSCiuc002dii.3 human [Q05996-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90366 mRNA Translation: AAA61335.1
AK313003 mRNA Translation: BAG35839.1
AF001550 Genomic DNA Translation: AAB67599.1
CH471228 Genomic DNA Translation: EAW66859.1
BC096304 mRNA Translation: AAH96304.1
BC096305 mRNA Translation: AAH96305.1
BC096306 mRNA Translation: AAH96306.1
BC096307 mRNA Translation: AAH96307.1
CCDSiCCDS10596.1 [Q05996-1]
CCDS73843.1 [Q05996-2]
PIRiA48833
RefSeqiNP_001277033.1, NM_001290104.1 [Q05996-2]
NP_003451.1, NM_003460.2 [Q05996-1]
UniGeneiHs.73982

3D structure databases

ProteinModelPortaliQ05996
SMRiQ05996
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113564, 8 interactors
IntActiQ05996, 2 interactors
MINTiQ05996
STRINGi9606.ENSP00000219593

PTM databases

iPTMnetiQ05996
PhosphoSitePlusiQ05996

Polymorphism and mutation databases

BioMutaiZP2
DMDMi466206

Proteomic databases

MaxQBiQ05996
PaxDbiQ05996
PeptideAtlasiQ05996
PRIDEiQ05996
ProteomicsDBi58364

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000574002; ENSP00000460971; ENSG00000103310 [Q05996-1]
ENST00000574091; ENSP00000458991; ENSG00000103310 [Q05996-2]
ENST00000638300; ENSP00000492703; ENSG00000284588 [Q05996-1]
ENST00000640487; ENSP00000491583; ENSG00000284588 [Q05996-2]
GeneIDi7783
KEGGihsa:7783
UCSCiuc002dii.3 human [Q05996-1]

Organism-specific databases

CTDi7783
EuPathDBiHostDB:ENSG00000103310.10
GeneCardsiZP2
HGNCiHGNC:13188 ZP2
HPAiHPA011296
MalaCardsiZP2
MIMi182888 gene
neXtProtiNX_Q05996
OpenTargetsiENSG00000103310
Orphaneti404466 Female infertility due to zona pellucida defect
PharmGKBiPA37756
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHBM Eukaryota
ENOG4110GZY LUCA
GeneTreeiENSGT00530000063096
HOGENOMiHOG000059563
HOVERGENiHBG004379
InParanoidiQ05996
KOiK19927
OMAiEFPGKLK
OrthoDBiEOG091G02TE
PhylomeDBiQ05996
TreeFamiTF332794

Enzyme and pathway databases

ReactomeiR-HSA-1300644 Interaction With The Zona Pellucida

Miscellaneous databases

GeneWikiiZP2
GenomeRNAii7783
PROiPR:Q05996
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103310 Expressed in 43 organ(s), highest expression level in oocyte
CleanExiHS_ZP2
GenevisibleiQ05996 HS

Family and domain databases

InterProiView protein in InterPro
IPR001507 ZP_dom
IPR017977 ZP_dom_CS
PfamiView protein in Pfam
PF00100 Zona_pellucida, 1 hit
PRINTSiPR00023 ZPELLUCIDA
SMARTiView protein in SMART
SM00241 ZP, 1 hit
PROSITEiView protein in PROSITE
PS00682 ZP_1, 1 hit
PS51034 ZP_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiZP2_HUMAN
AccessioniPrimary (citable) accession number: Q05996
Secondary accession number(s): B2R7J2
, Q4VAN9, Q4VAP0, Q4VAP1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 7, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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