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Protein

Neuronal acetylcholine receptor subunit alpha-7

Gene

Chrna7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-7
Gene namesi
Name:Chrna7
Synonyms:Acra7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2348 Chrna7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 230ExtracellularSequence analysisAdd BLAST208
Transmembranei231 – 255HelicalSequence analysisAdd BLAST25
Transmembranei262 – 280HelicalSequence analysisAdd BLAST19
Transmembranei296 – 317HelicalSequence analysisAdd BLAST22
Topological domaini318 – 469CytoplasmicSequence analysisAdd BLAST152
Transmembranei470 – 490HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi81S → T: 10-fold more potently inhibited by the alpha-conotoxin RgIA. 1 Publication1
Mutagenesisi141L → D: No change in inhibition by the alpha-conotoxin RgIA. 1 Publication1
Mutagenesisi433L → A: Abolishes dependency on RIC3 for functional expression. 1 Publication1
Mutagenesisi435K → A: No effect on dependency on RIC3 for functional expression. 1 Publication1
Mutagenesisi440V → A: Impairs dependency on RIC3 for functional expression. 1 Publication1
Mutagenesisi445N → A: Slightly impairs dependency on RIC3 for functional expression. 1 Publication1
Mutagenesisi446R → A: Impairs dependency on RIC3 for functional expression. 1 Publication1
Mutagenesisi447F → A: Impairs dependency on RIC3 for functional expression. 1 Publication1
Mutagenesisi448R → A: Impairs dependency on RIC3 for functional expression. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL4980
GuidetoPHARMACOLOGYi468

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000000036923 – 502Neuronal acetylcholine receptor subunit alpha-7Add BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi46N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi90N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi133N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi150 ↔ 164By similarity
Disulfide bondi212 ↔ 213Associated with receptor activationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ05941
PRIDEiQ05941

PTM databases

iPTMnetiQ05941
PhosphoSitePlusiQ05941
SwissPalmiQ05941

Interactioni

Subunit structurei

Homopentamer (By similarity). Interacts with RIC3; which is required for proper folding and assembly (PubMed:15927954, PubMed:16120769). Interacts with LYPD6 (By similarity). Interacts with the alpha-conotoxin RgIA (PubMed:25740413). Interacts with alpha-conotoxins ImI and ImII (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P606154EBI-79422,EBI-7516391From Bungarus multicinctus.

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-233 Neuronal nicotinic acetylcholine receptor complex, alpha7
CPX-237 Neuronal nicotinic acetylcholine receptor complex, alpha7-beta2
IntActiQ05941, 3 interactors
STRINGi10116.ENSRNOP00000045255

Chemistry databases

BindingDBiQ05941

Structurei

3D structure databases

ProteinModelPortaliQ05941
SMRiQ05941
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
HOGENOMiHOG000006756
HOVERGENiHBG003756
InParanoidiQ05941
KOiK04809
OrthoDBiEOG091G0R20
PhylomeDBiQ05941

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q05941-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCGGRGGIWL ALAAALLHVS LQGEFQRRLY KELVKNYNPL ERPVANDSQP
60 70 80 90 100
LTVYFSLSLL QIMDVDEKNQ VLTTNIWLQM SWTDHYLQWN MSEYPGVKNV
110 120 130 140 150
RFPDGQIWKP DILLYNSADE RFDATFHTNV LVNASGHCQY LPPGIFKSSC
160 170 180 190 200
YIDVRWFPFD VQQCKLKFGS WSYGGWSLDL QMQEADISSY IPNGEWDLMG
210 220 230 240 250
IPGKRNEKFY ECCKEPYPDV TYTVTMRRRT LYYGLNLLIP CVLISALALL
260 270 280 290 300
VFLLPADSGE KISLGITVLL SLTVFMLLVA EIMPATSDSV PLIAQYFAST
310 320 330 340 350
MIIVGLSVVV TVIVLRYHHH DPDGGKMPKW TRIILLNWCA WFLRMKRPGE
360 370 380 390 400
DKVRPACQHK PRRCSLASVE LSAGAGPPTS NGNLLYIGFR GLEGMHCAPT
410 420 430 440 450
PDSGVVCGRL ACSPTHDEHL MHGAHPSDGD PDLAKILEEV RYIANRFRCQ
460 470 480 490 500
DESEVICSEW KFAACVVDRL CLMAFSVFTI ICTIGILMSA PNFVEAVSKD

FA
Length:502
Mass (Da):56,503
Last modified:May 1, 2007 - v2
Checksum:i289A30498C7B9A58
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5UMH9Q5UMH9_RAT
Neuronal acetylcholine receptor sub...
Chrna7
531Annotation score:
A0A0G2K5T5A0A0G2K5T5_RAT
Neuronal acetylcholine receptor sub...
Chrna7
440Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti447F → N in AAB25224 (PubMed:7678857).Curated1
Sequence conflicti469R → P in AAB25224 (PubMed:7678857).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S53987 mRNA Translation: AAB25224.2
L31619 mRNA Translation: AAC33136.1
AY574256 mRNA Translation: AAS90352.1
PIRiT01378
RefSeqiNP_036964.3, NM_012832.3
UniGeneiRn.9698

Genome annotation databases

GeneIDi25302
KEGGirno:25302
UCSCiRGD:2348 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S53987 mRNA Translation: AAB25224.2
L31619 mRNA Translation: AAC33136.1
AY574256 mRNA Translation: AAS90352.1
PIRiT01378
RefSeqiNP_036964.3, NM_012832.3
UniGeneiRn.9698

3D structure databases

ProteinModelPortaliQ05941
SMRiQ05941
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-233 Neuronal nicotinic acetylcholine receptor complex, alpha7
CPX-237 Neuronal nicotinic acetylcholine receptor complex, alpha7-beta2
IntActiQ05941, 3 interactors
STRINGi10116.ENSRNOP00000045255

Chemistry databases

BindingDBiQ05941
ChEMBLiCHEMBL4980
GuidetoPHARMACOLOGYi468

PTM databases

iPTMnetiQ05941
PhosphoSitePlusiQ05941
SwissPalmiQ05941

Proteomic databases

PaxDbiQ05941
PRIDEiQ05941

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25302
KEGGirno:25302
UCSCiRGD:2348 rat

Organism-specific databases

CTDi1139
RGDi2348 Chrna7

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
HOGENOMiHOG000006756
HOVERGENiHBG003756
InParanoidiQ05941
KOiK04809
OrthoDBiEOG091G0R20
PhylomeDBiQ05941

Miscellaneous databases

PROiPR:Q05941

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACHA7_RAT
AccessioniPrimary (citable) accession number: Q05941
Secondary accession number(s): Q53YK2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 1, 2007
Last modified: November 7, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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